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Gastrotomy closure with a new tissue anchoring device:A porcine survival study

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World Journal of Gastroenterology 2011年第13期17卷 1732-1738页

作者： Carlos Guarner-Argente Henry Córdova Graciela Martínez-Pallí Ricard Navarro-Ripoll Antonio Rodríguez-d’Jesús cristina Rodríguez de miguel Mireia Beltrán Gloria Fernández-Esparrach Endoscopy Unit Hospital Clínic CIBERehd Barcelona 08026 Spain Anesthesiology department Hospital Clínic CIBERehd Barcelona 08026 Spain

AIM: To evaluate the feasibility, reproducibility and efficacy of a new tissue anchoring device in a porcine survival model. METHODS: Gastrotomies were performed using a needle-knife and balloon dilator in 10 female Yorkshire pigs weighing 30-35 kg. Gastric closure was attempted using a new tissue anchoring device. The tightness of the closure was confirmed by means of air insufflation and the ability to maintain gastric distension with stability in peritoneal pressure measured with a Veress needle. All animals were monitored daily for signs of peritonitis and sepsis over 14 d. During necropsy, the peritoneal cavity and the gastric access site were ***: Transgastric access, closure and 14 d survival was achieved in all pigs. The mean closure time was 18.1 ± 19.2 min and a mean of 2.1 ± 1 devices were used. Supplementary clips were necessary in 2 cases. The closure time was progressively reduced (24.8 ± 13.9 min in the first 5 pigs vs 11.4 ± 5.9 min in the last 5, P = NS). At necropsy, the gastric access site was correctly closed in all cases with all brace-bars present. One device was misplaced in the mesocolon. Minimal adhesions were observed in 3 pigs and signs of mild peritonitis and adhesions in one. CONCLUSIONS: The use of this new tissue anchoring device in porcine stomachs is feasible, reproducible and effective and requires a short learning curve.

关键词： Gastrotomy Closure Suture Survival Porcine model Notes

Three-dimensional trees for virtual globes

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International Journal of Digital Earth 2014年第10期7卷 789-810页

作者： cristina Rebollo Inmaculada Remolar Jesus Gumbau miguel Chover Institute of New Imaging Technologies Universitat Jaume ICastellonSpain

One of the problems in virtual globes technologies is the real-time representation of vegetal *** forest or garden representations,the low detailed plants produce a lack of *** techniques are required to achieve accurate interactive visualisation due to the great number of polygons the vegetal species *** article presents a multi-resolution model based on a geometric representation of vegetal species that allows the application to perform the progressive transmission of the model,that is,the transmission of a simple representation followed by successive refinements of *** has a hardware-oriented design in order to obtain interactive frame *** geometric data of the objects are stored in the graphics processing unit and,moreover,the change from one approximation to another is obtained by performing mathematical calcula-tions in this graphics *** multi-resolution model presented here enables instancing:as many vegetal species as desired can be rendered with different levels of detail,while all of them are accessing the same geometric *** model has been used to build a real-time representation of a not imaginary scenario.

关键词： virtual globes real-time rendering vegetal species level of detail graphics hardware progressive transmission

Stepwise magnetization reversal of geometrically tuned in diameter Ni and FeCo bi-segmented nanowire arrays

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Nano Research 2019年第7期12卷 1547-1553页

作者： Ester M. Palmero miguel Mendez Silvia Gonzalez cristina Bran Victor Vega Manuel Vazquez Victor M. Prida Institute of Materials Science of Madrid (ICMM-CSIC) Madrid 28049Spain department of Physics University of OviedoFederico Garda Lorca 18Oviedo 33007Spain Division of Permanent Magnets and Applications IMDEA NanoscienceMadrid 28049Spain

Magnetization reversal processes of hexagonal dense arrays of bi-segmented Ni and Fe50Co50 nanowires consisting of two well defined diameters (45 and 80 nm) have been *** nanowires were grown inside of tailored pores of anodic alumina templates by combined anodization,atomic layer deposition (ALD) and electrodeposition *** experiments have allowed to identify their two-step magnetization reversal process ascribed to the respective segments of different *** is concluded from the differential susceptibility observed in the hysteresis loops,contrary to those for nanowires with homogeneous *** results are also confirmed by the first-order reversal curve (FORC) distribution diagrams,where an elongation parallel to the interaction axis around two coercive field values is obtained,which is correlated to the difference in diameter of the two *** well-defined two-step magnetization reversal process through the nanowire diameter design is thought to be very useful for the advanced control of the remagnetization in arrays of magnetic multidomain systems.

关键词： Bi-segmented nanowires nickel iron cobalt alloy stepwise magnetization reversal first-order reversal curve (FORC)

The Strawberry Fruit Fra a Allergen Functions in Flavonoid Biosynthesis

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Molecular Plant 2010年第1期3卷 113-124页

作者： cristina Munoz Thomas Hoffmann Nieves Medina Escobar Felix Ludemann miguel A. Botella Victoriano Valpuesta Wilfried Schwab departamento de Biologia Molecular y Bioquimica Universidad de Malaga 29071 Malaga Spain Biomolecular Food Technology Technische Universit~t M0nchen Hochfeldweg 1 85354 Freising Germany

The strawberry Fra a I allergen is a homolog of the major birch pollen allergen Bet v 1. It is synthesized by red ripe fruits of Fragaria x ananassa while white fruits of a mutant genotype, which is known to be tolerated by individuals affected by allergy, are devoid of it. Proteomic analyses have shown that Fra a 1 is down-regulated in the tolerated white- fruited genotype along with enzymes of the anthocyanin pigment pathway. In this study, we report the spatial and tem- poral expression of three Fra a genes that encode different isoforms, and the transient RNAi-mediated silencing of the Fra a genes in strawberry fruits of the red-fruited cultivar Elsanta with an ihpRNA construct. As a consequence of reduced levels of Fra a mRNAs, fruits were obtained that produced significantly decreased levels of anthocyanins and upstream metabolites. This effect is consistent with the parallel down-regulation of the phenylalanine ammonia lyase （FaPAL） and to a lesser extent of the chalcone synthase （FaCHS） transcript levels also found in these fruits. In naturally occurring white- fruited genotypes of F. chiloensis and F. vesca, Fra a transcript levels are higher than those of the red-fruited varieties, likely to compensate for the low expression levels of FaPAL and FaCHS in these mutant genotypes. The results demonstrate that Fra a expression is directly linked to flavonoid biosynthesis and show that the Fra a allergen has an essential biological function in pigment formation in strawberry fruit.

关键词： Fragaria flavonoid anthocyanin phenylpropanoid allergen pathogenesis-related proteins.

First Serological Evidence on Endemicity of HEV Infection in Wild Boar(Sus scrofa) Populations from Portugal

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Virologica Sinica 2018年第2期33卷 197-200页

作者： Daniel Goncalves Joao Pereira-Vaz Vitor Duque Victor Bandeira Carlos Fonseca Ana Donato cristina Luxo Ana miguel Matos Laboratory of Virology Faculty of Pharmacy University of Coimbra 3000-548 Coimbra Portugal Laboratory of Molecular Biology Clinical Pathology UnitHospital and University Centre of Coimbra3000-602 Coimbra Portugal Infectious Diseases department Hospital and University Centre of Coimbra 3000-075 Coimbra Portugal Biology department and CESAM University of Aveiro3810-193 Aveiro Portugal Clinical Laboratory Faculty of Pharmacy University of Coimbra 3000-548 Coimbra Portugal Research Centre on Chemical Processes Engineering and Forest Products (CIEPQF) Faculty of Pharmacy University of Coimbra 3030-790 Coimbra Portugal

Increasing number of hepatitis E virus（HEV）infection has been described in industrialized countries,with infection occurring as a sporadic disease,usually associated with travel to endemic regions or small outbreaks... 详细信息

关键词：感染 HEV 血清学葡萄牙野猪证据特性人口

Intestinal preparation prior to capsule endoscopy administration

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World Journal of Gastroenterology 2008年第37期14卷 5773-5775页

作者： Vicente Pons Beltrán cristina Carretero Begoa Gonzalez-Suárez Iaqui Fernández-Urien miguel Muoz-Navas Gastroenterology department Hospital La FeValencia 46009 Spain Gastroenterology department University of NavarraPamplona 31008 Spain Gastroenterology department Hospital del MarBarcelona 08036 Spain

In order to have an adequate view of the whole small intestine during capsule endoscopy, the preparation recommended consists of a clear liquid diet and an overnight fast. However, visualization of the small bowel during video capsule endoscopy can be impaired by intestinal contents. To improve mucosal visualization, some authors have evaluated different regimens of preparation. There is no consensus about the necessity of intestinal preparation for capsule endoscopy and it should be interesting to develop adequate guidelines to improve its efficacy and tolerability. Moreover, the effect of preparation type (purgative) on intestinal transit time is not clear. Since a bowel preparation cannot defini-tively improve its visibility (and theoretically the yield of the test), it is not routinely recommended.

关键词： Capsule endoscopy Intestinal preparation Polyethylene glycol Aqueous sodium phosphate

Performance Indicators for Sun-Tracking Systems: A Case Study in Spain

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Energy and Power Engineering 2014年第9期6卷 292-302页

作者： miguel de Simón-Martín cristina Alonso-Tristán Montserrat Díez-Mediavilla Solar and Wind Feasibility Technologies Research Group University of Burgos Burgos Spain Solar and Wind Feasibility Technologies Research Group University of Burgos Burgos Spain

Current PV cells technology takes advantage mainly from beam radiation. In this context the sun-trackers are such devices for efficiency improvement. On the other hand, projected shadows between devices make mandatory to increase the distances between mounting systems. Thus, the land’s capacity for mounting PV panels can be dramatically decreased. This fact conducts us to wonder where the optimum efficient point is. First, in this paper different types of sun-tracking systems are classified according to the movement they perform (cinematic classification). Further, three real PV installations-fixed, horizontal-axis tracking and dual-axis mount tracking—located in the same geographical area in Spain (they are approximately under the same weather conditions) are analyzed. These installations have been studied in order to establish which one is the most efficient and affordable—Specific Energy Production (SEP) and Performance Ratio (PR) analysis. PVGIS solar radiation estimate tool has been used for comparing the theoretical radiation potential on each plant. The land requirements have been considered in the analysis of the Ground Cover Ratio (GCR) and the Surface Performance Ratio (SPR). Moreover, comparing three main financial indicators let us carry out a financial study: Payback Time (PBT), Net Present Value (NPV) and Internal Rate of Return (IRR). In the case study, final annual energetic results demonstrate that the dual-axis plant shows a relevant SEP advantage, but if we take into account the land occupied for this sort of devices we find much more profitable the horizontal-axis sun-tracking system, with a SPR value 4.24% higher than the fixed system we have studied. Its PBT is also a 22% lower than the dual-axis tracking installation.

关键词： Sun-Tracker Cinematic Classification PVGIS GCR SPR PBT NPV IRR

The cold-induced factor CBF3 mediates root stem cell activity,regeneration,and developmental responses to cold

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Plant Communications 2023年第6期4卷 197-211页

作者： Pablo Perez-Garcia Ornella Pucciariello Alvaro Sanchez-Corrionero Javier Cabrera cristina del Barrio Juan Carlos del Pozo Mariano Perales Krzysztof Wabnik miguel A.Moreno-Risueno Centro de Biotecnologíay Genómica de Plantas(Universidad Politécnica de Madrid(UPM)–Instituto Nacional de Investigacióny Tecnología Agraria y Alimentaria–CSIC(INIA-CSIC)) MadridSpain

Plant growth and development involve the specification and regeneration of stem cell niches(SCNs).Although plants are exposed to disparate environmental conditions,how environmental cues affect developmental programs and stem cells is not well *** stem cells are accommodated in meristems in SCNs around the quiescent center(QC),which maintains their *** a combination of genetics and confocal microscopy to trace morphological defects and correlate them with changes in gene expression and protein levels,we show that the cold-induced transcription factor(TF)C-REPEAT BINDING FACTOR 3(CBF3),which has previously been associated with cold acclimation,regulates root development,stem cell activity,and ***3 is integrated into the SHORT-ROOT(SHR)regulatory network,forming a feedback loop that maintains SHR ***3 is primarily expressed in the root endodermis,whereas the CBF3 protein is localized to other meristematic tissues,including root *** of cbf3-1 using a wild-type CBF3 gene and a CBF3 fusion with reduced mobility show that CBF3 movement capacity is required for SCN patterning and regulates root ***,cold induces CBF3,affecting QC ***,exposure to moderate cold around 10℃–12℃promotes root regeneration and QC respecification in a CBF3-dependent manner during the recuperation *** contrast,CBF3 does not appear to regulate stem cell survival,which has been associated with recuperation from more acute cold(-4℃).We propose a role for CBF3 in mediating the molecular interrelationships among the cold response,stem cell activity,and development.

关键词： stem cells regeneration organ patterning protein movement cell fate low temperature

Esophageal capsule endoscopy

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World Journal of Gastroenterology 2008年第34期14卷 5254-5260页

作者： Ignacio Fernandez-Urien cristina Carretero Raul Armendariz miguel Muoz-Navas department of Gastroenterology University of Navarra

Capsule endoscopy is now considered as the first imaging tool for small bowel examination. Recently, new capsule endoscopy applications have been developed, such as esophageal capsule endoscopy and colon capsule endoscopy. Esophageal capsule endoscopy in patients with suspected esophageal disorders is feasible and safe, and could be also an alternative procedure in those patients refusing upper endoscopy. Although large-scale studies are needed to confirm its utility in GERD and cirrhotic patients, current results are encouraging and open a new era in esophageal examination.

关键词： Capsule endoscopy Esophagus Gastroe-sophageal reflux disease Varices Esophagoscopy PillCam ESO