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Hydrodynamic characteristics and particle tracking of 90° lateral intakes at an inclined river slope

Water Science and Engineering 2024年第2期17卷 197-208页

作者： Wei He Si-yuan Feng Jian Zhang Hong-wu Tang Yang Xiao sheng Chen chun-sheng liu College of Water Conservancy and Hydropower Engineering Hohai UniversityNanjing 210098China State Key Laboratory of Hydrology Water Resources and Hydraulic Engineering Hohai UniversityNanjing 210098China Collaborative Innovation Center on Water Safety and Water Science Hohai UniversityNanjing 210098China

Lateral intakes are common in *** pump effciency and sediment deposition are determined by the local hydrodynamic characteristics and mainstream division *** hydraulic characteristics of lateral withdrawal from inclined river slopes at different intake elevations should be ***,the division width exhibits significant vertical non-uniformity at an inclined river slope,which should be ***,a three-dimensional(3-D)hydrodynamic and particle-tracking model was developed with the Open Source Field Operation and Manipulation(Open FOAM),and the model was validated with physical model tests for 90°lateral withdrawal from an inclined side *** flow fields,withdrawal sources,and division widths were investigated with different intake bottom elevations,withdrawal discharges,and main channel *** study showed that under inclined side bank conditions,water entered the intake at an oblique angle,causing significant 3-D spiral flows in the intake rather than two-dimensional closed recirculation.A lower withdrawal discharge,a lower bottom elevation of the intake,or a higher main channel velocity could further strengthen this *** average division width and turbulent kinetic energy were smaller under inclined side bank conditions than under vertical bank *** a low intake bottom elevation,a low withdrawal discharge,or a high main channel velocity,the sources of lateral withdrawal were in similar ranges near the local inclined bank in the vertical *** inclined slope conditions,sediment deposition near the intake entrance could be reduced,compared to that under vertical slope *** results provide hydrodynamic and sediment references for engineering designs for natural rivers with inclined terrains.

关键词： Lateral withdrawal Hydrodynamic characteristics Particle tracking Inclined river slope Bottom elevation of intake OpenFOAM

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Polyphenols from Securidaca inappendiculata alleviated acute lung injury in rats by inhibiting oxidative stress sensitive pathways

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Chinese Herbal Medicines 2021年第3期13卷 381-388页

作者： Cong-lan Ji sheng Dai Hong liu Ji-yang Dong chun-sheng liu Jian Zuo Department of Pharmacy Anhui College of Traditional Chinese MedicineWuhu 241000China Department of Electronic Science Xiamen UniversityXiamen 361005China Yijishan Hospital Wannan Medical CollegeWuhu 241000China Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institution(Wannan Medical College) Wuhu 241000China Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicines in Southern Anhui(Wannan Medical College) Wuhu 241000China

Objective:Securidaca inappendiculata is a medicinal plant frequently used in the treatment of inflammatory diseases in south *** this study,we aimed to explore its bioactive constituent which contributes to the anti-inflammatory ***:Polyphenol-enriched and polyphenol-deprived fractions(PRF and PDF,respectively)were separated from the ethanolic extract by HPD300 macroporous resin-based method,and their antiinflammatory activities were investigated on a lipopolysaccharide(LPS)-induced acute lung injury(ALI)model in *** possible mechanism of action in alleviating acute inflammation was studied using RAW264.7 ***:Both Folin-Ciocalteu and^(1)H nuclear magnetic resonance(NMR)analyses showed that polyphenolic content in PRF was approximately 10 times higher than that of PDF,and this observation reflected in their antioxidative *** but not PDF significantly decreased the level of malondialdehyde,suppressed the expression of nicotinamide phosphoribosyltransferase(NAMPT)protein,and improved the severity of ALI in *** at 10μg/mL effectively downregulated the expression of proteins NAMPT,HMGB1,TLR4,and p-p65,and scavenged the intracellular reactive oxygen species(ROS)in LPS-primed RAW264.7 cells.N-acetyl-L-cysteine exhibited similar inhibitory effects on ROS production and NAMPT-mediated TLR4/NF-κB activation in vitro,whereas nicotinamide mononucleotide antagonized all the changes induced by PRF during ***:As an antioxidant,PRF exhibited potent anti-inflammatory activity under both in vivo and in vitro conditions by downregulating NAMPT and TLR4/NF-κ***,polyphenols were identified as important bioactive constituents in *** targeting oxidative stress-sensitive proinflammatory pathways.

关键词： antioxidant polyphenols reactive oxygen species(ROS) Securidaca inappendiculata Hassk TLR4/NF-κB

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Effect of RNA interference on WD101 gene of Schistosoma japonicum

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Asian Pacific Journal of Tropical Medicine 2018年第2期11卷 136-140页

作者： Peng Zhang Gang Feng Wei-Na Zhang Ying-Ying Zhang chun-sheng liu Department of Clinical Laboratory Yijishan Hospital of Wanan Medical College

Objective: To study the effect of RNA interference(RNAi) on WD101 gene and its effect on the expression of WD101 mRNA and protein in Schistosoma ***: Doublestranded RNA(dsRNA) WD101 gene and control gene(lacZ) were generated by in vitrotranscription and transfected into mechanically transformed *** total RNA and protein were isolated simultaneously using TRIzol *** expression levels of mRNA and the protein were determined by quantitative real-time PCR(qPCR) and Western blotting, *** injected dsRNA-electroporated schistosomula into BALB/c mouse six weeks, the male and female reproductive organs were observed and measured under the confocal laser scanning ***: After 1, 3 and 5 d of RNAi, WD101 mRNA level was decreased by 15%, 39%, and 58% in experiment group compared to that in control group; meanwhile, WD101 protein level was decreased by 11%, 28%, and 43% in experiment group compared to that in control *** were significantly more sperms in testicular lobes in experiment group than that in control group, while there were no significant differences in terms of ovary and vitelline glands between two ***: The ds WD101-RNAi can effectively induce suppression of WD101 gene expression at both mRNA and protein ***101 gene might be a reproduction-related gene in Schistosoma japonicum.

关键词： Schistosoma japonicum RNA interference WD101

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Enzymatic synthesis of myricetin 3-O-galactoside through a whole-cell biocatalyst

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Chinese Herbal Medicines 2020年第4期12卷 384-389页

作者： Zhen Xu Wei-qing He chun-sheng liu Jian-qiang Kong State Key Laboratory of Bioactive Substance and Function of Natural Medicines&NHC Key Laboratory of Biosynthesis of Natural Products Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing 102488China NHC Key Laboratory of Biotechnology of Antibiotics Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China

Objective:Myricetin 3-O-galactoside is an active compound with pharmaceutical *** insufficient supply of this compound becomes a bottleneck in the druggability study of myricetin ***,it is necessary to develop a biosynthetic process for myricetin 3-O-galactoside through metabolic ***:Two genes OcSUS1 and OcUGE1 encoding sucrose synthase and UDP-glucose 4-epimerase were introduced into BL21(DE3)to reconstruct a UDP-D-galactose(UDP-Gal)biosynthetic pathway in Escherichia *** resultant chassis strain was able to produce ***,a flavonol 3-O-galactosyltransferase DkFGT gene was transformed into the chassis strain producing *** artificial pathway for myricetin 3-O-galactoside biosynthesis was thus constructed in ***:The obtained engineered strain was demonstrated to be capable of producing myricetin 3-Ogalactoside,reaching 29.7 mg/***:Biosynthesis of myricetin 3-O-galactoside through engineered *** could be *** result lays the foundation for the large-scale preparation of myricetin 3-O-galactoside.

关键词： engineered Escherichia coli flavonol 3-O-galactosyltransferase galactosyltransferase myricetin 3-O-galactoside

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CCT4 suppression inhibits tumor growth in hepatocellular carcinoma by interacting with Cdc20

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Chinese Medical Journal 2021年第22期134卷 2721-2729页

作者： Feng Li chun-sheng liu Ping Wu An-sheng Ling Qi Pan Xiao-Ning Li Department of Gastroenterology The First People’s Hospital of AnqingAnqingAnhui 246000China Department of Laboratory Medicine Yijishan Hospital of Wannan Medical CollegeWuhuAnhui 241001China Department of Rehabilitation Medicine The Sixth People’s Hospital of AnqingAnqingAnhui 246000China

Background:The chaperonin containing t-complex(CCT)proteins play an important role in cell cycle-related protein degradation in yeast and *** role of the chaperonin containing t-complex 4(CCT4),one subtype of CCT proteins,in the progress of hepatocellular carcinoma(HCC)was not fully ***,we aimed to explore the mechanisms of CCT4 in ***:In this study,we used the UALCAN platform to analyze the relationship between CCT4 and HCC,and the association of CCT4 with the overall survival(OS)of HCC patients was also ***4 expression in HCC tumor tissues and normal tissues was also determined by western blot(WB)*** vector was used to knock down the CCT4 expression,and quantitative polymerase chain reaction and WB were used to determine the level of CCT4 in HCC cell *** counting kit-8(CCK-8)and 5-ethynyl-20-deoxyuridine(EdU)assays were used to detect the cell proliferation,and flow cytometry(FCM)was performed to evaluate the effect of CCT4 on the apoptosis of HCC ***-immunoprecipitation(co-IP)assay and WB were used to explore the mechanisms of CCT4 regulating the growth of *** were calculated from at least three replicate experiments and expressed as mean±standard ***’s t test,paired t test,and Kaplan–Meier analysis were used to compare across different ***:We found CCT4 was upregulated in HCC tissues compared with normal tissues,and its high expression was associated with poor prognosis(P<0.001).CCT4 was significantly increased in HCC tumor tissues compared with normal tissues(0.98±0.12 vs.0.23±0.05,t=7.73,P<0.001).After being transfected with CCT4 short-hairpin RNA(shRNA),CCT4 was decreased in mRNA level and protein level in both Huh7(mRNA level:0.41±0.07 vs.1.01±0.11,t=8.09,P=0.001;protein level:0.61±0.03 vs.0.93±0.07,t=7.19,P=0.002)and Hep3b cells(mRNA level:0.55±0.11 vs.1.04±0.15,t=4.51,P=0.011;protein level:0.64±0.10 vs.0.95±0.08,t=4.32,P=0.012).CCK8 assay indicated that CCT4 knockdo

关键词： Hepatocellular carcinoma Chaperonin containing t-complex 4(CCT4) Cdc20 Securin Bim

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HMGR, SQS, β-AS, and Cytochrome P450 Monooxygenase Genes in Glycyrrhiza uralensis

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Chinese Herbal Medicines 2015年第4期7卷 290-295页

作者： Rui Yang Bo-chuan Yuan Yong-sheng Ma Li-qiang Wang chun-sheng liu Ying liu School of Chinese Pharmacy Beijing University of Chinese Medicine

Glycyrrhiza uralensis is frequently used in traditional Chinese medicine. This plant contains a large amount of effective constituents, including triterpenoids and flavonoids. Among them, glycyrrhizin is believed to be the marker compound to evaluate the quality of G. uralensis based on Chinese Pharmacopoeia. Many studies showed that glycyrrhizin possesses various pharmacological activities, such as antibacterial, antiviral, antitumor, anti-inflammatory, and immune-stimulating activities. In this paper, we summarized the cloning, characterization, expression, and polymorphism analysis of several functional genes involved in glycyrrhizin biosynthesis in G. uralensis.

关键词： β-AS cytochrome P450 monooxygenase functional genes glycyrrhizin HMGR SQS

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Effects of antisense oligonucleotides of PKC-α on proliferation and apoptosis of HepG₂ in vitro

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Hepatobiliary & Pancreatic Diseases International 2005年第1期4卷 75-79页

作者： Bao-Hua Zhu Zhen-Xiang Yao Shao-Jun Luo Li-Ming Jiang Jiang-Wei Xiao sheng-chun liu Jin-Biao liu Jian-Ming Sun Zhi-Yuan Pei Department of Hepatobiliary Surgery First Affiliated Hospital Chongqing University of Medical Sciences Chongqing 400016 China. baohuazhu@***

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignancies in China. The long-term survival rate of patients with HCC after prevention and management remains unsatisfactory. In order to provide a novel strategy to cure HCC, we investigated the effects of antisense oligonucleotides of PKC-alpha on proliferation and apoptosis of human hepatoma cell line HepG2 in vitro.METHODS: The human hepatocellular carcinoma cell line HepG2 was cultured and subcultured in RPMI1640 medium in vitro. PKC-alpha antisense oligonucleotides(asODN) of different concentrations with a random sequence as a control were transfected into HepG2 cells by lipofectin(LP). The cell growth index (GI) and the clone formation rate of HepG2 were detected by MTT colorimetric assay and soft agar assay, respectively. The apoptosis rate of HepG2 treated with PKC-alpha asODN was assayed by flow cytometry(FCM). The results were analyzed by SPSS 10.0 software.RESULTS: The GI of HepG2 transfected by PKC-alpha asODN with concentrations ranging from 0.10 micromol to 1.00 micromol were lower significantly than those of control groups (P<0.05). The clone formation rates of HepG2 transfected by PKC-alpha asODN from 0.05 micromol to 1.00 micromol were lower significantly than those of the control groups (P<0.01), and there was a dose-dependent relationship among them. The apoptosis rates of HepG2 treated with PKC-alpha asODN from 0.50 micromol to 1.00 micromol were significantly higher than those of the control groups.CONCLUSION: PKC-alpha asODN could inhibit the growth and proliferation of HepG2 and induce its apoptosis by blocking the cell signal transduction related to PKC-alpha in vitro, and may be potentially used in the prevention and management of recurrent and metastatic HCC.

关键词： hepatocellular carcinoma HepG2 PKC-α asODN

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Isolation and Complete Nucleotide Sequence of the Measles Virus IMB-1 Strain in China

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Virologica Sinica 2010年第6期25卷 381-389页

作者： Shao-hui MA Li-chun WANG Jian-sheng liu Hai-jing SHI Long-ding liu Qi-han LI Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical College Kunming 650118 China

The complete nucleotide sequence of the measles virus strain IMB-1,which was isolated in China,was *** in other measles viruses,its genome is 15,894 nucleotides in length and encodes six *** full-length nucleotide sequence of the IMB-1 isolate differed from vaccine strains (including wild-type Edmonston strain) by 4%-5% at the nucleotide sequence *** isolate has amino acid variations over the full genome,including in the hemagglutinin and fusion *** report is the first to describe the full-length genome of a genotype H1 strain and provide an overview of the diversity of genetic characteristics of a circulating measles virus.

关键词： Complete nucleotide sequence Genotype H1 Measles virus (MV)

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海面随机起伏对噪声场空间特性的影响规律

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物理学报 2017年第1期66卷 121-131页

作者：周建波朴胜春刘亚琴祝捍皓哈尔滨工程大学水声技术重点实验室哈尔滨150001 哈尔滨工程大学水声工程学院哈尔滨150001 浙江海洋大学海洋科学与技术学院舟山316022

对于1 k Hz以上声波,海面起伏会对浅海声传播产生显著影响,现有的噪声预报模型在建模过程中基本没有考虑海面起伏的影响.针对这一问题,本文基于传输理论建立了随机起伏界面下噪声场垂直相关性和指向性模型,仿真分析了海面起伏对噪声强... 详细信息

对于1 k Hz以上声波,海面起伏会对浅海声传播产生显著影响,现有的噪声预报模型在建模过程中基本没有考虑海面起伏的影响.针对这一问题,本文基于传输理论建立了随机起伏界面下噪声场垂直相关性和指向性模型,仿真分析了海面起伏对噪声强度、垂直相关性与指向性的影响.结果表明,对于表面噪声,海面随机起伏使声波能量从中间阶简正波向低阶和高阶简正波转移,而对噪声强度起主要贡献的一般是中间阶简正波,所以海面起伏使得噪声强度减弱;简正波之间能量的耦合导致垂直平面上不同掠射角方向上到达的声波响应发生变化,经由海面反射大掠射角到达的声波响应以及中小角度到达的声波响应变弱,而经由海底反射大掠射角到达的声波响应变强;海面随机起伏还会扰动各阶简正波相位,使不同阶简正波互相关性变弱,致使噪声场的空间相关性也变弱.

关键词：传输理论随机起伏环境噪声空间特性

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Transforming growth factor-beta 1 enhances discharge activity of cortical neurons

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Neural Regeneration Research 2025年第2期20卷 548-556页

作者： Zhihui Ren Tian Li Xueer liu Zelin Zhang Xiaoxuan Chen Weiqiang Chen Kangsheng Li Jiangtao sheng Department of Microbiology and Immunology Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular ImmunopathologyShantou University Medical CollegeShantouGuangdong ProvinceChina Department of Neurosurgery First Affiliated Hospital of Shantou University Medical CollegeShantouGuangdong ProvinceChina

Transforming growth factor-beta 1(TGF-β1)has been extensively studied for its pleiotropic effects on central nervous system *** neuroprotective or neurotoxic effects of TGF-β1 in specific brain areas may depend on the pathological process and cell types ***-gated sodium channels(VGSCs)are essential ion channels for the generation of action potentials in neurons,and are involved in various neuroexcitation-related ***,the effects of TGF-β1 on the functional properties of VGSCs and firing properties in cortical neurons remain *** this study,we investigated the effects of TGF-β1 on VGSC function and firing properties in primary cortical neurons from *** found that TGF-β1 increased VGSC current density in a dose-and time-dependent manner,which was attributable to the upregulation of Nav1.3 *** VGSC current density and Nav1.3 expression were significantly abolished by preincubation with inhibitors of mitogen-activated protein kinase kinase(PD98059),p38 mitogen-activated protein kinase(SB203580),and Jun NH2-terminal kinase 1/2 inhibitor(SP600125).Interestingly,TGF-β1 significantly increased the firing threshold of action potentials but did not change their firing rate in cortical *** findings suggest that TGF-β1 can increase Nav1.3 expression through activation of the ERK1/2-JNK-MAPK pathway,which leads to a decrease in the firing threshold of action potentials in cortical neurons under pathological ***,this contributes to the occurrence and progression of neuroexcitatory-related diseases of the central nervous system.

关键词： central nervous system cortical neurons ERK firing properties JNK Nav1.3 p38 transforming growth factor-beta 1 traumatic brain injury voltage-gated sodium currents

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