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Exploring the anti-aging effects of chlorogenic acid and the underlying mechanisms based on a Caenorhabditis elegans model

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Journal of Traditional Chinese Medical Sciences 2023年第2期10卷 208-217页

作者： Yuqian Yang Xu Chen Danyang Ye Chuanyu Wei Xinxin Pang Chuchu Kong Yongsheng Fang Hongliu Yang Yuanyuan Zhang Yonggang Liu School of Chinese Materia Madica Beijing University of Chinese MedicineBeijing102488China Krannert Institute of Cardiology Indiana UniversityBurmingtonIndiana46517USA

Objective:To explore the anti-aging effects of chlorogenic acid(CGA)and the underlying mechanisms based on a Caenorhabditis elegans(C.elegans)model.Methods:The anti-agingactivityofCGAwasstudied basedon thebodylength,exercisebehavior,lipofuscin content,antioxidative stress ability,swallowing frequency,body-bending frequency,and head-swinging ability of C.elegans.Through DAF-16 nuclear translocation and SOD-3-GFP fluorescence experiments,the effects of CGA on ROS levels,antioxidant enzyme activities,MDA content,mutant-strain lifespan,and anti-aging molecular signaling pathways were explored,as well as the underlying mechanisms.Results:CGA improved multiple indices of the nematode:body length was increased(all P<0.001),head-swing frequency and body-bending frequency were increased(all P<0.05),nematode longevity was prolonged(P=0.0021),lipofuscin deposition in nematodes was slowed down(all P<0.001),the chemotaxis index was improved(P=0.0012),ROS levels were reduced(all P<.001),and SOD activity and MDA content were reduced(SOD:P=0.0017 between the low-concentration group and the control group,P<.001 between the high-concentration and medium-concentration groups and the control group;MDA:P=0.0135 between the low-concentration group and the control group,and P<0.001 between the high-concentration and medium-concentration groups and the control group).In addition,CGA also activated the DAF-16 transcription factor,promoted DAF-16 nuclear translocation under oxidative stress conditions(both P<0.001 between the high-concentration and medium-concentration groups and the control group),and increased SOD-3 gene expression in nematodes(all P<0.001).Conclusion:CGA plays an anti-aging role in C.elegans.The underlying mechanisms include activation of the insulin/IGF-1 signaling pathway and enhancement of DAF-16 activity.This study lays a foundation for further research into the anti-aging effects of CGA.

关键词： Chlorogenic acid Caenorhabditis elegans Antioxidant activity Anti-aging DAF-16

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基于跨语种声学分析的帕金森病检测方法

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电子与信息学报 2024年第2期46卷 546-554页

作者：季薇王传瑜吴迪李云郑慧芬南京邮电大学通信与信息工程学院南京210003 南京邮电大学计算机学院南京210023 南京医科大学附属老年医院南京210024

基于语音的帕金森病检测具有非介入式、成本较低和无创等优点。当前公开的帕金森病语音数据集大多来源于单一语种,存在数据容量不够大、受试者母语发音特点差异小等特点。单一语种数据集上训练的帕金森病检测模型在面对跨语种语音数据时... 详细信息

基于语音的帕金森病检测具有非介入式、成本较低和无创等优点。当前公开的帕金森病语音数据集大多来源于单一语种,存在数据容量不够大、受试者母语发音特点差异小等特点。单一语种数据集上训练的帕金森病检测模型在面对跨语种语音数据时,将出现性能下降。为避免语种差异带来的影响,提升模型在跨语种场景下的检测性能,该文引入对抗迁移学习和特征解耦的思想,提出一种帕金森病跨语种声学分析模型(CLSAM)。首先,将基于多头自注意力机制的Transformer编码块和多层神经网络级联,组成特征提取器模块,用于将从源域和目标域语音中提取的原始Fbank语音特征初步解耦为两个向量,即域不变病理信息表征向量和域信息表征向量;设计了目标任务不一致的双重对抗训练模块,显式地分离域不变病理信息和域信息;最终,提取跨语种语音数据中的域不变病理信息用于帕金森病检测。该文在公开的MaxLittle帕金森病语音数据集以及自采的帕金森病语音数据集上,采用十折交叉验证的方法验证了所提方法的有效性。实验结果表明:与传统机器学习方法以及现有的迁移学习算法相比,所提模型在跨语种场景中的检测准确率、敏感度和F1分数等性能均有明显提升。

关键词：跨语种声学分析帕金森病对抗迁移学习特征解耦

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A novel blue-light excitable Pr^(3+)doped(Sr,Ba)LaMgTaO6 phosphor for plant growth lighting

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Journal of Rare Earths 2023年第5期41卷 666-672,I0002页

作者： Xiaojiao Kang wei Lü Zhennan Zhu chuanyu Jia School of Electrical Engineering and Intelligentization Dongguan University of TechnologyDongguan 523808China

In this work,a series of Pr^(3+)ions doped(Sr,Ba)LaMgTaO_(6)phosphors were prepared and applied for plant growth lighting.Under 450 nm excitation,(Sr,Ba)LaMgTaO_(6):Pr^(3+)exhibits intense reddish emission at around 650 nm which is assigned to the3p0→3F2transition of Pr^(3+)ions.The luminescence intensity reaches to the maximum at 2.5 mol%Pr^(3+)doping content both in SrLaMgTaO_(6):Pr^(3+)and BaLaMgTaO_(6):Pr^(3+)systems.Meanwhile,the internal quantum efficiency(IQE)test shows that the IQE of the SrLaMgTaO_(6):0.025Pr^(3+)phosphor is 33.34%upon blue light excitation.The decay curves and temperature-dependent luminescence measurements of the(Sr,Ba)LaMgTaO_(6):Pr^(3+)phosphors were performed and investigated in detail.The red light emitting diodes(LEDs)were packaged using the SrLaMgTaO_(6):Pr^(3+)and BaLaMgTaO_(6):Pr^(3+)phosphors combined with a blue LED chip,which are welloverlapped with the absorption band of photosynthesis.Results indicate that the(Sr,Ba)LaMgTaO_(6):Pr^(3+)can serve as a component of the red light in plant-growth LEDs.

关键词： Phosphor Luminescence properties Plant-growth LEDs Rareearths

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Low-noise fluorescent detection of cardiac troponin I in human serum based on surface acoustic wave separation

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Microsystems & Nanoengineering 2023年第6期9卷 261-272页

作者： Xuan Chen chuanyu Zhang Xianglian Liu Yangchao Dong Hao Meng Xianming Qin Zhuangde Jiang Xueyong wei State Key Laboratory for Manufacturing Systems Engineering Xi'an Jiaotong UniversityXian 710049China Department of Microbiology School of Preclinical MedicineFourth Military Medical UniversityXi'an 710032China The Second Affliated Hospital of Xi'an Jiaotong University Xi'an 710004China School of Mechano-Electronic Engineering Xidian UniversityXi'an 710071China

Acute myocardial infarction(AMI)is a life-threatening disease when sudden blockage of coronary artery occurs.As the most specific biomarker,cardiac troponin I(cTnI)is usually checked separately to diagnose or eliminate AMI,and achieving the accurate detection of cTnI is of great significance to patients'life and health.Compared with other methods,fluorescent detection has the advantages of simple operation,high sensitivity and wide applicability.However,due to the strong fluorescence interference of biological molecules in body fluids,it is often difficult to obtain high sensitivity.In order to solve this problem,in this study,surface acoustic wave separation is designed to purify the target to achieve more sensitive detection performance of fluorescent detection.Specifically,the interference of background noise is almost completely removed on a microfluidic chip by isolating microbeads through acoustic radiation force,on which the biomarkers are captured by the immobilized detection probe.And then,the concentration of cTnI in human serum is detected by the fluorescence intensity change of the isolated functionalized beads.By this way,the detection limit of our biosensor calculated by 3σ/K method is 44 pg/mL and 0.34 ng/mL in PBS buffer and human serum respectively.Finally,the reliability of this method has been validated by comparison with clinical tests from the nephelometric analyzer in hospital.

关键词： cardiac separation noise

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基于改进浮动单元的城市职住平衡时间尺度估计——以广州市为例

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城市交通 2024年第2期22卷 74-85页

作者：孙泽彬陈子睿熊思敏陈传禹吴蔚广州市城市规划勘测设计研究院广东广州510060 广东省城市感知与监测预警企业重点实验室广东广州510060

已有研究对城市职住平衡的测度往往存在可塑性面积单元问题,且忽略了交通网络在职住联系中的媒介作用。提出一种改进的浮动单元法,构建基于道路和城市轨道交通网络的数据集,以时间为职住分析尺度生成浮动单元,运用手机信令数据对职住平... 详细信息

已有研究对城市职住平衡的测度往往存在可塑性面积单元问题,且忽略了交通网络在职住联系中的媒介作用。提出一种改进的浮动单元法,构建基于道路和城市轨道交通网络的数据集,以时间为职住分析尺度生成浮动单元,运用手机信令数据对职住平衡进行测度。以广州市为例,对就业中心和居住区的职住平衡时间尺度进行估计。研究发现:广州市中心城区职住平衡时间尺度分布呈圈层式空间特征,职住平衡时间尺度从一级就业中心向外围先减小再增大;城市轨道交通缩小了就业中心的职住平衡时间尺度;一级、二级(不处于一级就业中心的辐射范围内)和三级就业中心的职住平衡时间尺度分别为32~40 min、14~28 min和10~20 min,而居住组团的职住平衡时间尺度主要依附于就业中心且受到城市轨道交通的影响。最后,提出在合适的时间尺度下对职住空间结构进行评估和优化住房和就业等资源配置,并重视城市轨道交通对职住空间结构的影响。

关键词：交通规划职住平衡时间尺度浮动单元交通网络可达性城市轨道交通手机信令数据广州市

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水稻抗细菌性条斑病基因挖掘与初定位

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植物学报 2024年第1期59卷 1-9页

作者：方妍力田传玉苏如意刘亚培王春连陈析丰郭威纪志远浙江师范大学生命科学学院金华321004 中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程北京100081

由稻黄单胞菌稻生致病变种(Xanthomonas oryzae pv. oryzicola,Xoc)引起的细菌性条斑病(BLS)是水稻(Oryza sativa)生产上的重要病害,近年来其发病呈快速上升趋势,特别是在我国南方(包括江苏、浙江、福建和广东等地)稻区危害严重。种植... 详细信息

由稻黄单胞菌稻生致病变种(Xanthomonas oryzae pv. oryzicola,Xoc)引起的细菌性条斑病(BLS)是水稻(Oryza sativa)生产上的重要病害,近年来其发病呈快速上升趋势,特别是在我国南方(包括江苏、浙江、福建和广东等地)稻区危害严重。种植抗病品种是防治BLS最理想的措施,但目前生产上严重缺乏可用于育种的优异抗病基因资源。通过人工接种鉴定筛选水稻种质资源,挖掘到2份高抗BLS材料(M1和D1)。多菌株系接种结果表明,M1具有非小种特异性广谱抗病(RNS BSR)特征。经遗传群体分析表明,栽培稻M1携带单个显性抗BLS新基因Xo-3。通过混池测序和关联分析,将Xo-3基因初步定位在2号染色体上的一段候选区域内。抗BLS种质资源的挖掘及其抗性遗传基础的解析,将有助于理解水稻-Xoc互作机理,从而培育抗BLS水稻新品种和制定科学的防治BLS策略。

关键词：细菌性条斑病种质资源抗病基因水稻稻黄单胞菌

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Efficient Simultaneous Detection of Metabolites Based on Electroenzymatic Assembly Strategy

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Biomedical Engineering Frontiers 2023年第1期4卷 31-41页

作者： Anran Zheng Chao Li Shengkai Xu Zhen Guo chuanyu Li Changsong Zhang Jia Yao Zhiqi Zhang Jinze Li Lutao Du Shasha Zhao Chuanxin Wang wei Zhang Lianqun Zhou CAS Key Lab of Bio-Medical Diagnostics Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesSuzhou 215163China School of Biomedical Engineering(Suzhou) Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230026China Suzhou Hospital Affiliated Hospital of Medical SchoolNanjing UniversitySuzhou 215153Jiangsu ProvinceChina Suzhou CASENS Co.Ltd. Suzhou 215163China Ji Hua Laboratory Foshan 528000China Department of Clinical Laboratory The Second Hospital of Shandong UniversityJinan 250033ShandongChina

Objective and Impact Statement:We describe an electroenzymatic mediator(EM)sensor based on an electroenzymatic assembly peak separation strategy,which can efficiently realize the simultaneous detection of 3 typical cardiovascular disease(CVD)metabolites in 5μl of plasma under one test.This work has substantial implications toward improving the efficiency of chronic CVD assessment.Introduction:Monitoring CVD of metabolites is strongly associated with disease risk.Independent and time-consuming detection in hospitals is unfavorable for chronic CVD management.Methods:The EM was flexibly designed by the cross-linking of electron mediators and enzymes,and 3 EM layers with different characteristics were assembled on one electrode.Electrons were transferred under tunable potential;3 metabolites were quantitatively detected by 3 peak currents that correlated with metabolite concentrations.Results:In this study,the EM sensor showed high sensitivity for the simultaneous detection of 3 metabolites with a lower limit of 0.01 mM.The linear correlation between the sensor and clinical was greater than 0.980 for 242 patients,and the consistency of risk assessment was 94.6%.Conclusion:Metabolites could be expanded by the EM,and the sensor could be a promising candidate as a home healthcare tool for CVD risk assessment.

关键词： flex linking consuming

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Multiple virus sorting based on aptamer-modified microspheres in a TSAW device

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微系统与纳米工程(英文) 2023年第3期9卷 309-318页

作者： Xianglian Liu Xuan Chen Yangchao Dong chuanyu Zhang Xiaoli Qu Yingfeng Lei Zhuangde Jiang Xueyong wei State Key Laboratory for Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’an 710049China Department of Microbiology the Fourth Military Medical UniversityXi’an 710032China

Due to the overlapping epidemiology and clinical manifestations of flaviviruses,differential diagnosis of these viral diseases is complicated,and the results are unreliable.There is perpetual demand for a simplified,sensitive,rapid and inexpensive assay with less cross-reactivity.The ability to sort distinct virus particles from a mixture of biological samples is crucial for improving the sensitivity of diagnoses.Therefore,we developed a sorting system for the subsequent differential diagnosis of dengue and tick-borne encephalitis in the early stage.We employed aptamer-modified polystyrene(PS)microspheres with different diameters to specifically capture dengue virus(DENV)and tick-borne encephalitis virus(TBEV),and utilized a traveling surface acoustic wave(TSAW)device to accomplish microsphere sorting according to particle size.The captured viruses were then characterized by laser scanning confocal microscopy(LSCM),field emission scanning electron microscopy(FE-SEM)and reverse transcription-polymerase chain reaction(RT‒PCR).The characterization results indicated that the acoustic sorting process was effective and damage-free for subsequent analysis.Furthermore,the strategy can be utilized for sample pretreatment in the differential diagnosis of viral diseases.

关键词： diagnosis epidemiology sorting

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Integrated microdevice with a windmill-like hole array for the clog-free,efficient,and self-mixing enrichment of circulating tumor cells

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Microsystems & Nanoengineering 2022年第1期8卷 291-304页

作者： Hao Li Jinze Li Zhiqi Zhang Zhen Guo Changsong Zhang Zixu Wang Qiuquan Guo Chao Li chuanyu Li Jia Yao Anran Zheng Jingyi Xu Qingxue Gao wei Zhang Lianqun Zhou School of Biomedical Engineering(Suzhou) Division of Life Sciences and MedicineUniversity of Science and Technology of China260026 HefeiChina CAS Key Lab of Bio-Medical Diagnostics Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of Sciences215163 SuzhouChina Ji Hua Laboratory 528000 FoshanChina Department of Laboratory Medicine The Affiliated Suzhou Science and Technology Town HospitalNanjing Medical University215153 SuzhouChina Shenzhen Institute for Advanced Study University of Electronic Science and Technology of China518000 ShenzhenChina Suzhou CASENS Co. Ltd215163 SuzhouChina Jinan Guoke Medical Technology Development Co. Ltd250001 JinanChina

Circulating tumor cells(CTCs)have tremendous potential to indicate disease progression and monitor therapeutic response using minimally invasive approaches.Considering the limitations of affinity strategies based on their cost,effectiveness,and simplicity,size-based enrichment methods that involve low-cost,label-free,and relatively simple protocols have been further promoted.Nevertheless,the key challenges of these methods are clogging issues and cell aggregation,which reduce the recovery rates and purity.Inspired by the natural phenomenon that the airflow around a windmill is disturbed,in this study,a windmill-like hole array on the SU-8 membrane was designed to perturb the fluid such that cells in a fluid would be able to self-mix and that the pressure acting on cells or the membrane would be dispersed to allow a greater velocity.In addition,based on the advantages of fluid coatings,a lipid coating was used to modify the membrane surface to prevent cell aggregation and clogging of the holes.Under the optimal conditions,recovery rates of 93%and 90%were found for A549 and HeLa cells in a clinical simulation test of our platform with a CTC concentration of 20-100 cells per milliliter of blood.The white blood cell(WBC)depletion rate was 98.7%(n=15),and the CTC detection limit was less than 10 cells per milliliter of blood(n=6).Moreover,compared with conventional membrane filtration,the advantages of the proposed device for the rapid(2 mL/min)and efficient enrichment of CTCs without clogging were shown both experimentally and theoretically.Due to its advantages in the efficient,rapid,uniform,and clog-free enrichment of CTCs,our platform offers great potential for metastatic detection and therapy analyses.

关键词： logging free aggregation

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Study on Integrated Design of Building Facilities in Scenic Areas

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Journal of Landscape Research 2013年第9期5卷 5-6,10页

作者： NIE wei KANG chuanyu DONG Liang School of Architecture Southwest Jiaotong University

The authors begin with traditional architectural design methodology,review basic theories and development trends of architectural designs from the ancient times to the present.On the basis of combining traditional oriental integral view,modern architectural design and landscape design theories,current construction of building facilities in scenic areas are analyzed,integrated design of the building facilities is proposed,and"integrated design system"further analyzed to offer effective references and theoretical support for the design and construction of building facilities in scenic areas.

关键词： Scenic area Method of design Building facilities Integrated design

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