Multiple virus sorting based on aptamer-modified microspheres in a TSAW device
作者机构：State Key Laboratory for Manufacturing Systems EngineeringXi’an Jiaotong UniversityXi’an 710049China Department of Microbiologythe Fourth Military Medical UniversityXi’an 710032China
出 版 物：《微系统与纳米工程(英文)》 (Microsystems & Nanoengineering)
年 卷 期：2023年第9卷第3期
页 面：309-318页
核心收录：
学科分类：07[理学] 070205[理学-凝聚态物理] 08[工学] 0710[理学-生物学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0804[工学-仪器科学与技术] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 100401[医学-流行病与卫生统计学] 0702[理学-物理学] 10[医学]
基 金：This project is financially supported by the National Key R&D Program of China(2020YFB2009002) the Program for Innovation Team of Shaanxi Province(2021TD-23)
主 题：diagnosis epidemiology sorting
摘 要：Due to the overlapping epidemiology and clinical manifestations of flaviviruses,differential diagnosis of these viral diseases is complicated,and the results are *** is perpetual demand for a simplified,sensitive,rapid and inexpensive assay with less *** ability to sort distinct virus particles from a mixture of biological samples is crucial for improving the sensitivity of ***,we developed a sorting system for the subsequent differential diagnosis of dengue and tick-borne encephalitis in the early *** employed aptamer-modified polystyrene(PS)microspheres with different diameters to specifically capture dengue virus(DENV)and tick-borne encephalitis virus(TBEV),and utilized a traveling surface acoustic wave(TSAW)device to accomplish microsphere sorting according to particle *** captured viruses were then characterized by laser scanning confocal microscopy(LSCM),field emission scanning electron microscopy(FE-SEM)and reverse transcription-polymerase chain reaction(RT‒PCR).The characterization results indicated that the acoustic sorting process was effective and damage-free for subsequent ***,the strategy can be utilized for sample pretreatment in the differential diagnosis of viral diseases.
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