Efficient Simultaneous Detection of Metabolites Based on Electroenzymatic Assembly Strategy
作者机构：CAS Key Lab of Bio-Medical DiagnosticsSuzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesSuzhou 215163China School of Biomedical Engineering(Suzhou)Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230026China Suzhou HospitalAffiliated Hospital of Medical SchoolNanjing UniversitySuzhou 215153Jiangsu ProvinceChina Suzhou CASENS Co.Ltd.Suzhou 215163China Ji Hua LaboratoryFoshan 528000China Department of Clinical LaboratoryThe Second Hospital of Shandong UniversityJinan 250033ShandongChina
出 版 物：《Biomedical Engineering Frontiers》 (生物医学工程前沿（英文）)
年 卷 期：2023年第4卷第1期
页 面：31-41页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 081101[工学-控制理论与控制工程] 0811[工学-控制科学与工程] 081102[工学-检测技术与自动化装置]
基 金：This work was supported by the National Key R&D Program of China(2021YFB3201202) the National Natural Science Foundation of China(nos.22005331,52275581,and 82372142) the Key Research and Development Program of Jiang su Province(nos.BE2022739,BE2020768,and SBE2022780090) the Youth Innovation Promotion Association of CAS(nos.2019322 and Y2022088) the Instrument Developing Project of the Chinese Academy of Sciences(nos.YJKYYQ20200046 and ZDKYYQ20210004) the Science and Technology Development Program of Suzhou(no.SJC2021019)
摘 要：Objective and Impact Statement:We describe an electroenzymatic mediator(EM)sensor based on an electroenzymatic assembly peak separation strategy,which can efficiently realize the simultaneous detection of 3 typical cardiovascular disease(CVD)metabolites in 5μl of plasma under one *** work has substantial implications toward improving the efficiency of chronic CVD ***:Monitoring CVD of metabolites is strongly associated with disease *** and time-consuming detection in hospitals is unfavorable for chronic CVD ***:The EM was flexibly designed by the cross-linking of electron mediators and enzymes,and 3 EM layers with different characteristics were assembled on one *** were transferred under tunable potential;3 metabolites were quantitatively detected by 3 peak currents that correlated with metabolite ***:In this study,the EM sensor showed high sensitivity for the simultaneous detection of 3 metabolites with a lower limit of 0.01 *** linear correlation between the sensor and clinical was greater than 0.980 for 242 patients,and the consistency of risk assessment was 94.6%.Conclusion:Metabolites could be expanded by the EM,and the sensor could be a promising candidate as a home healthcare tool for CVD risk assessment.
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