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Effects of chronic exposure to nonylphenol on locomotor activity and social behavior in zebrafish(Danio rerio)

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Journal of Environmental Sciences 2010年第9期22卷 1435-1440页

作者： Jigang Xia,Cuijuan Niu ,Xuejiao pei Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering,College of Life Sciences,Beijing Normal University,100875 Beijing,China. [a] Ministry of Education Key Laboratory of Biodiversity Science Ecological Engineering College of Life Sciences Beijing Normal University 100875 Beijing China

Nonylphenol （NP）, a xenoestrogen ubiquitously found in aquatic ecosystems, is of high environmental concern. The present work assessed the effects of exposure to NP on locomotor activity and social behavior in male and female zebrafish （Danio rerio）, to evaluate the possible hazard of NP to fish behavior and to pursue a potential biomarker of NP contamination. Fish were randomly divided into six groups. Five groups were receiving 0.1, 1, 10, 50 and 100 μg/L nominal concentrations of NP for 60 days, respectively. A sixth control group was given the same treatment as the other five groups, but no NP. Locomotor activity, aggressive behavior, group preference and leaving shoal were examined. NP exposure showed marked influence on locomotor activity of the male zebrafish, whereas that of the female was not significantly affected by NP. Aggressive behavior and group preference were significantly affected by NP exposure in both male and female groups. Locomotor activity and aggressive behavior of the male and group preference of both male and female zebrafish were clearly inhibited at 100 μg/L NP. No significant alteration in leaving shoal was observed under NP exposure. The results suggested that changes in locomotor activity and aggressive behavior of the male and group preference of both male and female may be used as an ecologically relevant integrative biomarker of NP contamination.

关键词： chronic toxicity nonylphenol biomarker zebrafish

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Self-assembly of ZnⅡ-benzendicarboxylate coordination polymers with Phen

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Self-assembly of ZnⅡ-benzendicarboxylate coordination polym...

miR-1269 targeting SOX7 on proliferation and mechanism of esophageal cancer cells

第十六届全国金属有机化学学术讨论会

作者： pei-Zhou Li,Yuyou Chen,Yangang Bi,Yu chong,Zixiang Chi,Xiaoming Lu~* Department of Chemistry,Capital Normal University,Beijing,100037,China.

＜正＞The self-assembly of coordination polymers with non-covalent bonds,such as coordination bonds,hydrogen bonds,π----πpacking interactions,have attracted chemist’s much attention in recent *** is arisen not only for their various intriguing topological structures but also for their unexpected properties for potential practical applications in material chemistry,such as gas sorption,storage and separations,heterogeneous catalysis,molecular recognition,

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miR-1269 targeting SOX7 on proliferation and mechanism of es...

Controversies regarding and perspectives on clinical utility of biomarkers in hepatocellular carcinoma

第十二届全国免疫学学术大会

作者： dong pei Hebei Medical University Fourth Hospital

Object to determine the expression of miR-1269 and SOX7 in human esophageal cancer cells and identify the role of miR-1269 in the invasion of KYES30 cells by regulation the expression of ***:1 Expression level of mir-1269 in esophageal carcinoma tissues and para-cancer tissues were detected by *** analyzed the correlation between mir-1269 expression and clinic pathlological characteristics of patients with esophageal cancer.2 After the transfection of miR-1269 and the application of their inhibitors,the expression of mir-1269 and sox7 were determined using qRT-PCR and Western ***,transwell mirgration and matrigel invasion chamber assays were used to detect the cell proliferetion,migration and invasion abilities of esophageal cancer cells withmir-1269 low-expression or low-expression,respectively.3 After the transfection of miR-1269 and the application of their inhibitors,the expression of DVL3,GSK3 b,TCF7,Bi-Phospho-GSK3 B,cmy-c were determined using WesHJtern *** dual luciferase reporter system,mir-1269 was proved to inhibit SOX7 expression by binging ***:1 RT-qPCR assay demonstrated that the expression level of miR-1269 in esophageal carcinoma tissues was significantly higher than that in normal esophageal tissues.2 Results of qRT-PCR and Western blot showed that the over-expression of mir-1269 down-regulated the expression of *** proliferetion,migration and invasion of cells was promoted by over-expresion of *** dual luciferase reporter system,mir-1269 was proved to inhibit sox7 expression by binging to 3,UTR of SOX7 ***:MiR-1269 may negatively regulte the expression of sox7 binging to 3,UTR of SOX7 mRNA and inhibit the invasion of human esophageal carcinoma cells.

关键词： mir-1269 SOX7 Wnt/β-catenin esophagus cancer

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World Journal of Gastroenterology 2016年第1期22卷 262-274页

作者： pei-pei Song Ju-Feng Xia Yoshinori Inagaki Kiyoshi Hasegawa Yoshihiro Sakamoto Norihiro Kokudo Wei Tang Hepato-Biliary-Pancreatic Surgery Division Department of SurgeryGraduate School of Medicinethe University of Tokyo

The prevalence of hepatocellular carcinoma(HCC)worldwide parallels that of persistent infection with the hepatitis B virus(HBV)and/or hepatitis C virus(HCV).According to recommendations by the World Health Organization guidelines for HBV/HCV,alpha-fetoprotein(AFP)testing and abdominal ultrasound should be performed in routine surveillance of HCC every 6 mo for high-risk *** examinations have also been recommended worldwide by many other HCC guidelines over the past few *** recent years,however,the role of AFP in HCC surveillance and diagnosis has diminished due to advances in imaging *** was excluded from the surveillance and/or diagnostic criteria in the HCC guidelines published by the American Association for the Study of Liver Diseases in 2010,the European Association for the Study of the Liver in 2012,and the National Comprehensive Cancer Network in *** biomarkers,including the Lens culinaris agglutinin-reactive fraction of AFP(AFP-L3),des-γ-carboxyprothrombin,Dickkopf-1,midkine,and micro RNA,are being studied in this ***,increasing attention has focused on the clinical utility of biomarkers as pre-treatment predictors for tumor recurrence and as post-treatment *** and tissue-based biomarkers and genomics may aid in the diagnosis of HCC,determination of patient prognosis,and selection of appropriate ***,further studies are needed to better characterize the accuracy and potential role of these approaches in clinical practice.

关键词： Hepatocellular carcinoma Biomarker Guideline Surveillance Diagnosis Prognosis

基于层控数据驱动的层间多次波预测与压制方法及其应用

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石油物探 2023年第5期62卷 912-924页

作者：裴云龙杨金龙蒋波袁刚中石化石油物探技术研究院有限公司江苏南京211103

复杂的火成岩或膏岩层强反射界面产生的层间多次波对一次波有强烈的干涉效应,精准识别层间多次波并对其进行预测和压制是地震资料处理的技术瓶颈之一。常规的叠前数据驱动的层间多次波预测与压制方法需要预测每个反射界面产生的多次波,... 详细信息

复杂的火成岩或膏岩层强反射界面产生的层间多次波对一次波有强烈的干涉效应,精准识别层间多次波并对其进行预测和压制是地震资料处理的技术瓶颈之一。常规的叠前数据驱动的层间多次波预测与压制方法需要预测每个反射界面产生的多次波,效果不佳且运算量非常大。为此,提出基于层控数据驱动的层间多次波预测与压制方法,从层间多次波正演模拟出发,分析了对目的层有影响的层间多次波的产生来源和形成的地质条件,再利用井标定的井震匹配情况和构造模型多次波正演模拟记录进行对比确定,将产生多次波的主要反射界面作为层控模型层,从而减少对所有多次波进行预测的运算量,再采用逐层预测和压制的思路,保证预测的层间多次波的有效性,最后,采用保护指定有效地层的最小二乘自适应匹配相减技术进行层面多次波的压制。模拟数据和实际资料的处理结果表明,该方法能够有效预测和压制有影响的层间多次波,从而消除假构造,提高了地层构造、断裂和缝洞型储层的成像精度。

关键词：层间多次波识别预测与压制自适应相减叠前偏移成像

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The Study of Virtual Enterprise＇s Leader

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Chinese Business Review 2005年第7期4卷 77-78,81页

作者： Zhonghua Zhao Xueliang pei Shenyang Ligong University

With the fast changing of environment, virtual enterprises become a kind of new manufacture mode day by day. In the course of establishment and operation of virtual enterprises, the leader＇s status is very important. The study of the leader＇s characteristic, function and how to discern will contribute to realize the leader＇s function and guarantee the healthy and fast development of virtual enterprises.

关键词： virtual enterprises leader discern

离子型共价有机框架材料的制备及其对染料的吸附性能研究

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化学通报 2024年第3期87卷 349-354,257页

作者：裴秀陈洪卓李亚明罗刚酒泉职业技术学院、甘肃省太阳能发电系统工程重点实验室酒泉735000 兰州理工大学有色金属先进加工与回收国家重点实验室兰州730000 山东中再生环境检测有限公司济南250000

茜素红(Alizarin Red S)具有毒性高、结构复杂以及化学需氧量(COD)大的特点,是水中难处理的污染物之一。本文采用三醛基间苯三酚和溴化乙锭作为构筑单元,在溶剂热条件下合成一种二维离子型共价有机框架材料TpEB-COF,并通过X射线衍射、... 详细信息

茜素红(Alizarin Red S)具有毒性高、结构复杂以及化学需氧量(COD)大的特点,是水中难处理的污染物之一。本文采用三醛基间苯三酚和溴化乙锭作为构筑单元,在溶剂热条件下合成一种二维离子型共价有机框架材料TpEB-COF,并通过X射线衍射、扫描电镜和能量色散谱等对其进行结构表征。将制备的TpEB-COF作为固体吸附剂应用对水中茜素红的吸附,考察了吸附时间和pH对吸附过程的影响。结果表明,TpEB-COF对于茜素红的吸附符合准二级动力学方程和Langmuir吸附模型,吸附效率为82.8%,最大吸附量为828mg/g。本研究可为COFs材料的设计和合成提供参考,拓展了离子型COFs材料的应用范围。

关键词：离子型共价有机框架茜素红吸附污水处理

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Acetyl-11-keto-β-boswellic acid inhibits proliferation and induces apoptosis of gastric cancer cells through the phosphatase and tensin homolog/Akt/cyclooxygenase-2 signaling pathway

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World Journal of Gastroenterology 2020年第38期26卷 5822-5835页

作者： Meng-Xue Sun Xiao-Pu He pei-Yun Huang Qi Qi Wei-Hao Sun Gao-Shuang Liu Jie Hua Department of Geriatric Gastroenterology The First Affiliated Hospital of Nanjing Medical UniversityNanjing 210000Jiangsu ProvinceChina Nanjing Medical University Nanjing 210000Jiangsu ProvinceChina Department of Gastroenterology The First Affiliated Hospital of Nanjing Medical UniversityNanjing 210000Jiangsu ProvinceChina

BACKGROUND Gastric cancer is one of the most common malignant tumors of the digestive system worldwide,posing a serious danger to human ***(COX)-2 plays an important role in the carcinogenesis and progression of gastric ***-11-keto-β-boswellic acid(AKBA)is a promising drug for cancer therapy,but its effects and mechanism of action on human gastric cancer remain *** To evaluate whether the phosphatase and tensin homolog(PTEN)/Akt/COX-2 signaling pathway is involved in the anti-tumor effect of AKBA in gastric *** Human poorly differentiated BGC823 and moderately differentiated SGC7901 gastric cancer cells were routinely cultured in Roswell Park Memorial Institute 1640 medium supplemented with 10%fetal bovine serum and 1%penicillin/*** cancer cell proliferation was determined by methyl thiazolyl tetrazolium colorimetric *** was measured by flow *** migration was assessed using the wound-healing *** of Bcl-2,Bax,proliferating cell nuclear antigen,PTEN,p-Akt,and COX-2 were detected by Western blot analysis.A xenograft nude mouse model of human gastric cancer was established to evaluate the anti-cancer effect of AKBA RESULTS AKBA significantly inhibited the proliferation of gastric cancer cells in a dose-and time-dependent manner,inhibited migration in a time-dependent manner,and induced apoptosis in a dose-dependent manner in vitro;it also inhibited tumor growth in *** up-regulated the expression of PTEN and Bax,and downregulated the expression of proliferating cell nuclear antigen,Bcl-2,p-Akt,and COX-2 in a dose-dependent *** PTEN inhibitor bpv(Hopic)reversed the high expression of PTEN and low expression of p-Akt and COX-2 that were induced by *** Akt inhibitor MK2206 combined with AKBA downregulated the expression of p-Akt and COX-2,and the combined effect was better than that of AKBA *** AKBA inhibits the proliferation and migration and p

关键词： Acetyl-11-keto-β-boswellic acid Gastric cancer Cell proliferation Apoptosis Cyclooxygenase-2 Tumor xenograft

Purification and characterization of alkaline chitinase from Paenibacillus pasadenensis CS0611

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Chinese Journal of Catalysis 2017年第4期38卷 665-672页

作者： Xiaoxiao Guo pei Xu Minhua Zong Wenyong Lou Laboratory of Applied Biocatalysis School of Food Science and Engineering South China University of Technology Guangzhou 510640 GuangdongChina State Key Laboratory of Pulp and Paper Engineering South China University of Technology Guangzhou 510640 Guangdong China

An extracellular chitinase produced by Paenibacillus pasadenensis CS0611was purified by ammoniumsulfate precipitation,HiTrap DEAE FF and HiLoad26/600Superdex200pg column *** apparent molecular mass determined by sodium dodecyl sulfate polyacrylamide gelelectrophoresis *** optimum pH and optimum temperature of the chitinase were5.0and50°C,*** enzyme showed high stability at alkaline pH values and temperaturesbelow40°***,the metal ions Mn2+,Mg2+,and Co2+inhibited activity of the *** was active on colloidal chitin with an apparent Km of4.41mg/mL and Vmax of1.08mg/*** spectrum analysis indicated that the chitinase reacted preferentially with the glucosidicbond between GlcNAc‐*** enzymatic hydrolysate was analyzed by high‐performance liquidchromatography and thin layer chromatography,and clearly showed that a subunit of(GlcNAc)2was the main hydrolysis product.

关键词： Chitinase Purification Characterization Paenibacillus pasadenensis CS0611

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