Acetyl-11-keto-β-boswellic acid inhibits proliferation and induces apoptosis of gastric cancer cells through the phosphatase and tensin homolog/Akt/cyclooxygenase-2 signaling pathway
作者机构:Department of Geriatric GastroenterologyThe First Affiliated Hospital of Nanjing Medical UniversityNanjing 210000Jiangsu ProvinceChina Nanjing Medical UniversityNanjing 210000Jiangsu ProvinceChina Department of GastroenterologyThe First Affiliated Hospital of Nanjing Medical UniversityNanjing 210000Jiangsu ProvinceChina
出 版 物:《World Journal of Gastroenterology》 (世界胃肠病学杂志(英文版))
年 卷 期:2020年第26卷第38期
页 面:5822-5835页
核心收录:
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金:Supported by the Natural Science Foundation of Jiangsu No.BK20171508
主 题:Acetyl-11-keto-β-boswellic acid Gastric cancer Cell proliferation Apoptosis Cyclooxygenase-2 Tumor xenograft
摘 要:BACKGROUND Gastric cancer is one of the most common malignant tumors of the digestive system worldwide,posing a serious danger to human ***(COX)-2 plays an important role in the carcinogenesis and progression of gastric ***-11-keto-β-boswellic acid(AKBA)is a promising drug for cancer therapy,but its effects and mechanism of action on human gastric cancer remain *** To evaluate whether the phosphatase and tensin homolog(PTEN)/Akt/COX-2 signaling pathway is involved in the anti-tumor effect of AKBA in gastric *** Human poorly differentiated BGC823 and moderately differentiated SGC7901 gastric cancer cells were routinely cultured in Roswell Park Memorial Institute 1640 medium supplemented with 10%fetal bovine serum and 1%penicillin/*** cancer cell proliferation was determined by methyl thiazolyl tetrazolium colorimetric *** was measured by flow *** migration was assessed using the wound-healing *** of Bcl-2,Bax,proliferating cell nuclear antigen,PTEN,p-Akt,and COX-2 were detected by Western blot analysis.A xenograft nude mouse model of human gastric cancer was established to evaluate the anti-cancer effect of AKBA RESULTS AKBA significantly inhibited the proliferation of gastric cancer cells in a dose-and time-dependent manner,inhibited migration in a time-dependent manner,and induced apoptosis in a dose-dependent manner in vitro;it also inhibited tumor growth in *** up-regulated the expression of PTEN and Bax,and downregulated the expression of proliferating cell nuclear antigen,Bcl-2,p-Akt,and COX-2 in a dose-dependent *** PTEN inhibitor bpv(Hopic)reversed the high expression of PTEN and low expression of p-Akt and COX-2 that were induced by *** Akt inhibitor MK2206 combined with AKBA downregulated the expression of p-Akt and COX-2,and the combined effect was better than that of AKBA *** AKBA inhibits the proliferation and migration and p