检索结果-南通市图书馆

物理学报 2005年第9期54卷 4141-4145页

作者：周慧君程木田刘绍鼎王取泉詹明生薛其坤武汉大学物理系武汉430072 中国科学院武汉物理与数学研究所武汉430071 中国科学院物理研究所国际量子结构中心北京100080

研究了线偏振脉冲光场激发下,单个各向异性InGaAs量子点的高偏振光发射.给出其偏振因子与两个正交本征态之间的交叉弛豫之间的关系式.分析表明,交叉弛豫随激发场强度增大,并导致偏振因子随激发场入射脉冲面积减小.

关键词：量子点单光子发射偏振度各向异性光子发射 InGaAs 交叉弛豫激发线偏振关系式

Simple and Rapid Method for Detection of Goose Parvovirus in the Field by Loop-Mediated Isothermal Amplification

在线全文

学校读者我要写书评

暂无评论

Simple and Rapid Method for Detection of Goose Parvovirus in...

Chinese expert consensus on organ protection of transplantation(2022 edition)

第五届中国畜牧科技论坛

作者： yang Jin-long yang Rui cheng An-chun wang ming-shu Fu Li-zhi yang Song-quan Zhang Su-hui yang liu Xu Zhi-yong Avian Diseases Research Center College of Veterinary Medicine of Sichuan Agricultural University Chongqing Academy of Animal Science Key Laboratory of Animal Diseases and Human Health of Sichuan Province College of Animal Sciences Henan Institute of Science and Technology

Background:Goose parvovirus(GPV) is a Dependovirus associated with latent infection and mortality in ***,it severely affects geese production *** objective of this study was to develop a loop-mediated isothermal amplification(LAMP) method for sensitive,rapid and inexpensive detection of GPV in the ***:A set of six specific primers was designed by targeting the GPV VP3 DNA. With Bst DNA polymerase large fragment,the target DNA could be amplified at 65℃as early as 20 min incubation in a simple water bath.A positive reaction was identified by the detection of the LAMP product by color change visible to the naked *** detection limit of the assay was 28 copies/μl of plasmid pVP3,with equally sensitivity and specificity detection by fluorescent quantitative real-time PCR(FQ-PCR).Conclusions: The high sensitivity,specificity,and simplicity,with no complicated equipment and technical training of the GPV LAMP assay,combined with a high throughput,make this method suitable for a broad spectrum both in field condition and in laboratory operation for specific detection of GPV infected.

关键词： GPV LAMP gosling real-time PCR

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2022年第6期21卷 516-526页

作者： Jian-hui Li Xiao Xu yan-Feng wang Hai-yang Xie Jing-Yu Chen Nian-Guo Dong Mitesh Badiwala Li-ming Xin Roberto Vanin Pinto Ribeiro Hao Yin Hao Zhang Jian-Zheng Zhang Feng Huo Jia-Yin yang Hong-Ji yang hui Pan Shao-Guang Li Yin-Biao Qiao Jia Luo Hao-Yu Li Jun-Jun Jia Hao Yu Han Liang Si-Jia yang Hao wang Zhong-yang liu Li-cheng Zhang Xiao-Yi Hu Hao Wu Yi-Qing Hu Pei-Fu Tang Qi-Fa Ye Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery Department of Liver TransplantationShulan(Hangzhou)HospitalZhejiang Shuren University School of MedicineHangzhou 310022China Department of Hepatobiliary and Pancreatic Surgery Hangzhou First People’s HospitalZhejiang University School of MedicineHangzhou 310000China Zhongnan Hospital of Wuhan University Institute of Hepatobiliary Diseases of Wuhan UniversityTransplant Center of Wuhan UniversityHubei Key Laboratory of Medical Technology on TransplantationWuhan 430062China NHC Key Laboratory of Combined Multi-organ Transplantation Hangzhou 310003China Wuxi Lung Transplantation Center Wuxi People’s Hospital Affiliated with Nanjing Medical UniversityWuxi 214023China Department of Cardiovascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Peter Munk Cardiac Centre Toronto General Hospital-University Health NetworkTorontoCanada School of Computer Engineering and Science Shanghai UniversityShanghai 200444China Organ Transplant Center Shanghai Changzheng HospitalShanghai 200003China Department of Orthopedics Chinese PLA General HospitalBeijing 100039China National Clinical Research Center for Orthopedics Sports Medicine&RehabilitationBeijing 100039China Department of Surgery General Hospital of Guangzhou Military Command of PLAGuangzhou 510040China Department of Liver Surgery Liver Transplantation CenterWest China Hospital of Sichuan UniversityChengdu 610041China Organ Transplantation Center Sichuan Provincial People’s Hospital and School of MedicineUniversity of Electronic Science and Technology of ChinaChengdu 610072China Department of Lung Transplantation The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Division of Hepatobiliary Pancreatic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China

Introduction Organ transplantation increases survival and improves qual-ity of life to many patients with end-stage organ ***-gan shortage is a worldwide problem that restricts organ trans-plantation[1].Organ procurement and preservation as well as ischemia-reperfusion injury(IRI)after transplantation are the im-portant factors affecting prognosis of *** the de-velopment of organ transplantation technology in the 20th cen-tury,organ protection technology has been a most promising con-cept in this *** preservation solutions such as the Collins solution,University of Wisconsin(UW)solution,and histidine-tryptophan-ketoglutarate(HTK)solution were developed sequen-tially[2],which developed rapidly in static cold storage(SCS)*** remains the standard preservation technique for organ transplantation[2].

关键词： organ preservation transplantation

人参皂苷Rh_(2)抑制人非小细胞肺癌细胞增殖的机制研究

在线全文

学校读者我要写书评

暂无评论

中草药 2022年第2期53卷 441-448页

作者：孙小单王天鸣李慧赵沛妍刘岩程颖吉林省肿瘤医院博士后科研工作站吉林长春130012 长春中医药大学中医学院吉林长春130117 吉林省肿瘤医院肿瘤转化医学实验室吉林长春130012 吉林省肿瘤医院胸部肿瘤内科吉林长春130012

目的考察人参皂苷Rh_(2)对人非小细胞肺癌A549及H460细胞增殖的影响,并进一步探讨其作用机制。方法人参皂苷Rh_(2)处理A549及H460细胞,采用CCK-8法及克隆形成实验检测药物对细胞增殖的作用;观察药物对细胞形态的影响,利用Annexin V-FITC... 详细信息

目的考察人参皂苷Rh_(2)对人非小细胞肺癌A549及H460细胞增殖的影响,并进一步探讨其作用机制。方法人参皂苷Rh_(2)处理A549及H460细胞,采用CCK-8法及克隆形成实验检测药物对细胞增殖的作用;观察药物对细胞形态的影响,利用Annexin V-FITC/碘化丙啶(PI)双染法检测细胞凋亡情况;利用乳酸检测试剂盒检测药物对细胞糖酵解产物即乳酸生成的影响;采用Western blotting法检测药物对线粒体凋亡蛋白[B淋巴细胞瘤2 (B-cell lymphoma 2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)、半胱氨酸蛋白酶-3 (Caspase-3)],糖酵解调节通路信号传导与转录激活因子3 (signal transducer and activator of transcription 3,STAT3)/c-myc及糖酵解途径关键酶丙酮酸激酶M2 (pyruvate kinase M2,PKM2)、乳酸脱氢酶A (lactate dehydrogenase A,LDHA)表达的影响。上调c-myc表达后,观察人参皂苷Rh;对A549及H460细胞乳酸水平的影响。结果人参皂苷Rh;可明显抑制A549及H460细胞的增殖能力及克隆形成能力(P<0.05、0.01、0.001),呈剂量和时间相关性。人参皂苷Rh;明显改变A549及H460细胞形态并诱导细胞凋亡(P<0.05),显著降低乳酸生成量(P<0.05、0.01)。人参皂苷Rh;可显著下调Bcl-2/Bax、PKM2、LDHA蛋白表达水平(P<0.05、0.01),上调Caspase-3蛋白表达水平(P<0.05),并抑制STAT3/c-myc通路活性(P<0.05、0.01)。上调c-myc表达后,人参皂苷Rh_(2)对A549及H460细胞乳酸水平的抑制作用减弱(P<0.05、0.01)。结论人参皂苷Rh_(2)显著抑制A549及H460细胞的增殖,其机制可能是通过调控线粒体凋亡蛋白、抑制STAT3/c-myc通路及糖酵解关键酶的表达干扰糖酵解,最终导致细胞凋亡。

关键词：人参皂苷Rh_(2) 非小细胞肺癌增殖凋亡糖酵解信号传导与转录激活因子3/c-myc通路

Erratum to: Exceptionally shear-stable and ultra-strong Ir-Ni-Ta high- temperature metallic glasses at micro/nano scales (vol 65, issue 2, page 501,2022)

在线全文

学校读者我要写书评

暂无评论

Science China Materials 2023年第3期66卷 1256-1256页

作者： Yu-Tian wang quan-Feng He Zi-Jian wang ming-Xing Li yan-hui liu Yong yang Bao-An Sun Wei-Hua wang Institute of Physics Chinese Academy of Sciences Beijing 100190 China College of Materials Science and Opto-Electronic Technology University of Chinese Academy of Sciences Beijing 100049 China Department of Mechanical Engineering City University of Hong Kong Kowloon Tong Kowloon Hong Kong China Songshan Lake Materials Laboratory Dongguan 523808 China

In the version of the article originally published in the volume 65, issue 2, 2022 of Sci. China Mater. (page 501-507, https://***/ 10.1007/s40843-021-1760-8), one affiliation of the first author (Yu-Tian wang) was missing, and the affiliations of the authors were incorrectly labeled. The corrected version of the authors’ affiliations is as below: Yu-Tian wang1,2†, quan-Feng He4†, Zi-Jian wang1 , ming-Xing Li1,3, yan-hui liu1,3, Yong yang4* , Bao-An Sun1,3* and Wei-Hua wang1,3 1 Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China 2 College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, China 3 Songshan Lake Materials Laboratory, Dongguan 523808, China 4 Department of Mechanical Engineering, City University of Hong Kong, Kowloon Tong, Kowloon, Hong Kong, China © Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022.

关键词：

基于MASTA和ANSYS的行星传动柔性分析

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

制造业自动化 2011年第1期33卷 100-104页

作者：刘越毛明王明成石彦辉中国北方车辆研究所车辆传动重点实验室北京100072

以某车侧传动行星减速器为研究对象,使用子结构法从ANSYS中提取的行星架刚度矩阵和节点位置信息,在专业设计软件MASTA中建立行星传动机构仿真分析模型,对行星传动机构进行柔性分析,将分析得到的数据作为边界条件导入ANSYS中,即可实现对... 详细信息

以某车侧传动行星减速器为研究对象,使用子结构法从ANSYS中提取的行星架刚度矩阵和节点位置信息,在专业设计软件MASTA中建立行星传动机构仿真分析模型,对行星传动机构进行柔性分析,将分析得到的数据作为边界条件导入ANSYS中,即可实现对行星架的有限元分析。这种方法解决了行星架有限元分析中边界条件不易确定的问题,并且可以得到考虑行星架实际刚度的齿轮安全系数和损伤率,提高了分析结果的准确性,为行星机构的仿真分析提供了新的思路。

关键词：行星传动 MASTA 柔性分析刚度矩阵节点位置信息

基于环境激励的大跨度斜拉桥模态参数和索力识别

在线全文

学校读者我要写书评

暂无评论

振动与冲击 2012年第16期31卷 157-163页

作者：叶锡钧颜全胜李健王卫锋朱添丰刘明慧华南理工大学土木与交通学院广州510641 伊利诺伊大学香槟分校工学院美国香槟61801

模态参数和索力是评估斜拉桥健康状态的关键参数。建立了崖门大桥的有限元模型并对其进行了基于环境激励的模态测试和拉索振动测试;提出了基于ERA的多参考点稳定图算法,设置不同的参考点,利用自然激励技术结合特征系统实现算法(NExT-ERA... 详细信息

模态参数和索力是评估斜拉桥健康状态的关键参数。建立了崖门大桥的有限元模型并对其进行了基于环境激励的模态测试和拉索振动测试;提出了基于ERA的多参考点稳定图算法,设置不同的参考点,利用自然激励技术结合特征系统实现算法(NExT-ERA)识别模态参数,通过阻尼比、基于输出矩阵的一致模态指标(CMI_O)和模态置信度(MAC)作为判别标准,识别出崖门大桥的竖向和横向模态参数,通过和增强频域分解法(EFDD)识别结果的比较,可知该算法具有良好的识别效果;分析了斜拉索与主梁的共振频率范围,通过二次拟合识别较长拉索的低阶频率,根据两种不同方法的索力识别结果可知,该桥的索力分布比较均匀对称。

关键词：环境激励斜拉桥模态参数识别多参考点稳定图索力识别

Effect of Metformin-Induced Stimulation on the Expression of Insulin Receptor Substrate 1 through Negative Regulation of P70S6k

在线全文

学校读者我要写书评

暂无评论

Reproductive and Developmental Medicine 2018年第1期2卷 15-20页

作者： hui-ming Ma Dong-Mei Chen Li Xiang Chao-Qun liu Qiao-Ni Hou yan-Tao He cheng Xin Yong-Fang Zhang Xiu-Ying Pei yan-Rong wang Xian Xu Department of Anatomy and Histology-Embryology Key Laboratory of Fertility Preservation and Maintenance of Ministry of EducationFertility Center for Reproductive Medicine of General HospitalNingxia Medical UniversityYinchuan 750004China Ningxia Human Stem Cell Institute of General Hospital Ningxia Medical UniversityYinchuan 750004China

Objective:The aim is to study the effects of metformin on the expression of 70 kDa ribosomal protein S6 kinase(P70S6k),insulin receptor substrate 1(IRS-1),and IRS-1Ser307 phosphorylation in human luteinized granulosa ***:Granulosa cells in the experimental group were cultured in M199 medium containing 0.1 mmol/L metformin for 24 h and those in control group were cultured in M199 *** expression levels of P70S6k and IRS-1 mRNA were detected by reverse-transcriptiom polymerase chain reaction(RT-PCR)and real-time PCR.P70S6k,IRS-1,p-ser307-IRS-1,and p-thr389-P70S6k protein expression levels were detected by immunofluorescence and western ***:P70S6k mRNA level was higher and IRS-1 was significantly lower in the experimental group than those in the control ***-1 and p-ser307-IRS-1 were expressed in cell plasma,and P70S6k and p-thr389-P70S6k were expressed in cell *** results of Western blot analysis indicated that the expression levels of P70S6k,p-thr389-P70S6k,IRS-1,and p-ser307-IRS-1 proteins had significant difference between the experimental group and the control *** to the control group,the relative intensity illustrated that the expression levels of P70S6K and p-thr389-P70S6k significantly increased in the experimental group;however,those of IRS-1 and p-ser307-IRS-1 proteins significantly ***:Metformin can inhibit the P70S6k mRNA and protein expression levels in the granulosa cells and improve insulin sensitivity by regulating IRS-1 expression through Akt/P70S6k/IRS-1-dependent pathway.

关键词： 70 kDa Ribosomal Protein S6 Kinase Human Luteinized Granulosa Cells Insulin Receptor Substrate 1 Metformin

The Lyman-alpha Solar Telescope(LST) for the ASO-S mission——Ⅱ. design of LST

在线全文

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2019年第11期19卷 33-48页

作者： Bo Chen hui Li Ke-Fei Song quan-Feng Guo Pei-Jie Zhang Ling-Ping He Shuang Dai Xiao-Dong wang Hai-Feng wang Chun-Long liu Hong-Ji Zhang Guang Zhang Yunqi wang Shi-Jie liu Hong-Xin Zhang Lei liu Shi-Lei Mao yang liu Jia-Hao Peng Peng wang Liang Sun yang liu Zhen-Wei Han yan-Long wang Kun Wu Guang-Xing Ding Peng Zhou Xin Zheng ming-Yi Xia Qing-Wen Wu Jin-Jiang Xie Ya Chen Shu-Mei Song Hong wang Bo Zhu Chang-Bo Chu Wen-Gang yang Li Feng Yu Huang Wei-Qun Gan Ying Li Jing-Wei Li Lei Lu Jian-Chao Xue Bei-Li Ying ming-Zhe Sun cheng Zhu Wei-Min Bao Lei Deng Zeng-Shan Yin Changchun Institute of Optics FineMechanics and PhysicsChinese Academy of SciencesChangchun 130033China State Key Laboratory of Applied Optics Changchun 130033China Key Laboratory of Dark Matter and Space Astronomy Purple Mountain ObservatoryChinese Academy of SciencesNanjing 210034China School of Astronomy and Space Science University of Science and Technology of ChinaHefei 230026China Xi’an Institute of Optics and Precision Mechanics Chinese Academy of SciencesXi’an 710119China Shandong University Weihai 264209China Innovation Academy for Microsatellites Chinese Academy of SciencesShanghai 201210China

As one of the three payloads for the Advanced Space-based Solar Observatory(ASO-S)mission,the Lyman-alpha(Lyα)Solar Telescope(LST)is composed of three instruments:a Solar Corona Imager(SCI),a LyαSolar Disk Imager(SDI)and a full-disk White-light Solar Telescope(WST).When working in-orbit,LST will simultaneously perform high-resolution imaging observations of all regions from the solar disk to the inner corona up to 2.5 R⊙(R⊙stands for the mean solar radius)with a spatial resolution of 4.8′′and 1.2′′for coronal and disk observations,respectively,and a temporal resolution of 30–120 s and 1–120 s for coronal and disk observations,*** maximum exposure time can be up to20 s due to precise pointing and image stabilization *** the three telescopes of LST,SCI is a dual-waveband coronagraph simultaneously and independently observing the inner corona in the HI Lyα(121.6±10 nm)line and white light(WL)(700±40 nm)wavebands by using a narrowband Lyαbeam splitter and has a field of view(FOV)from 1.1 to 2.5 R⊙.The stray-light suppression level can attain<10^-6 B⊙(B⊙is the mean brightness of the solar disk)at 1.1 R⊙and≤5×10^-8 B⊙at 2.5 R⊙.SDI and WST are solar disk imagers working in the Lyαline and 360.0 nm wavebands,respectively,which adopt an off-axis two-mirror reflective structure with an FOV up to 1.2 R⊙,covering the inner coronal edge area and relating to coronal *** present the up-to-date design for the LST payload.

关键词： telescopes—instrumentation high angular resolution—Sun flares—Sun coronal mass ejections(CMEs)—Sun UV radiation

An-Luo-Hua-Xian Pill Improves the Regression of Liver Fibrosis in Chronic Hepatitis B Patients Treated with Entecavir

在线全文

学校读者我要写书评

暂无评论

Journal of Clinical and Translational Hepatology 2023年第2期11卷 304-313页

作者： Yi-Qi liu Chi Zhang Jia-Wen Li Li-Hua Cao Zhan-Qing Zhang Wei-Feng Zhao Qing-Hua Shang Da-Zhi Zhang An-Lin Ma Qing Xie Hong-Lian Gui Guo Zhang Ying-Xia liu Jia Shang Shi-Bin Xie Jun Li Xu-Qing Zhang Zhi-Qiang Zou Yu-Ping Chen Zong Zhang ming-Xiang Zhang Jun cheng Fu-Chun Zhang Li-Hua Huang Jia-Bin Li Qing-Hua Meng Hai-Bin Yu Yu-Qiang Mi yan-Zhong Peng Zhi-Jin wang Li-ming Chen Fan-Ping Meng Wan-Hua Ren Lang Bai Yi-Lan Zeng Rong Fan Xian-Zhi Lou Wei-Feng Liang hui liu hui Zhuang Hong Zhao Gui-Qiang wang Department of Infectious Disease Center for Liver DiseasePeking University First HospitalBeijingChina Department of Hepatology The Third Hospital of QinhuangdaoQinhuangdaoHebeiChina Department of Infectious Disease Shanghai Public Health Clinical CenterFudan UniversityShanghaiChina Department of Infectious Disease Xinxiang Medical University Affiliated Third HospitalXinxiangHenanChina No.88 Hospital of Chinese People’s Liberation Army(PLA) JinanShandongChina Department of Infectious Diseases The Second Affiliated Hospital of Chongqing Medical UniversityChongqingChina Department of Infectious Disease China-Japan Friendship HospitalBeijingChina Department of Infectious Diseases Ruijin HospitalSchool of MedicineShanghai Jiaotong UniversityShanghaiChina Department of Gastroenterology The People’s Hospital of Guangxi Zhuang Autonomous RegionNanningGuangxiChina Department of Infectious Diseases The Third People’s Hospital of ShenzhenShenzhenGuangdongChina Department of Infectious Diseases The People’s Hospital of Henan ProvinceZhengzhouHenanChina Department of Infectious Disease The Third Affiliated Hospital of Sun-Yat Sen UniversityGuangzhouGuangdongChina Department of Infectious Diseases Jiangsu Province HospitalThe First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsuChina Department of Infectious Diseases The Southwest Hospital of Army Medical UniversityChongqingChina yantai Infectious Diseases Hospital YantaiShandongChina Department of Hepatology Baoding Infectious Diseases HospitalBaodingHebeiChina Department of Integrated Traditional Chinese Medicine(TCM)and Western Medicine Jinan Infectious Diseases HospitalShandong UniversityJinanShandongChina Department of Integrated TCM and Western Medicine in Hepatology The Sixth People’s Hospital of ShenyangShenyangLiaoningChina Beijing Ditan Hospital Capital Medical UniversityBeijingChina Department of Infectious Diseases Guangzhou Eighth People’s HospitalGuangzhou Medical UniversityGuangzhouGuangdon

Background and Aims:Chronic hepatitis B(CHB)can cause liver fibrosis and lead to cirrhosis and *** the effectiveness of antiviral therapy to reverse liver fibrosis is limited,We aimed to evaluate the effect of An-Luo-Hua-Xian pill(ALHX)on fibrosis regression in CHB patients treated with entecavir(ETV).Methods:Treatment-naïve patients with CHB were randomly treated with ETV alone or combined with ALHX(ETV+ALHX)between October 1,2013 and December 31,***,laboratory,and liver histology data before and after 78 weeks of treatment were *** Ishak fibrosis score(F)was used and fibrosis regression required a decrease in F of≥1 after ***:A total of 780 patients were enrolled,and 394 with a second liver biopsy after treatment were included in the per-protocol population,132 in ETV group and 262 in ETV+ALHX *** 78 weeks of treatment,the fibrosis regression rate in the ETV+ALHX group was significantly higher than that of the ETV group at baseline F≥3 patients:124/211(58.8%)vs.45/98(45.9%),p=*** percentage of patients with a decreased liver stiffness measurement(LSM)was higher in the ETV+ALHX group:156/211(73.9%)vs.62/98(63.%),p=*** regression analysis showed that ETV combined with ALHX was associated with fibrosis regression[odds ratio(OR)=1.94,p=0.018],and a family history of hepatocellular carcinoma was on the contrary.(OR=0.41,p=0.031).Conclusions:ETV combined with ALHX increased liver fibrosis regression in CHB patients.

关键词： Chronic hepatitis B Liver fibrosis Regression Randomized controlled trial