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Proteo-transcriptomic profiles reveal key regulatory pathways and functions of LDHA in the ovulation of domestic chickens(Gallus gallus)

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1449-1465页

作者： Ruixue Nie Wenhui Zhang Haoyu Tian Junying Li Yao Ling Bo Zhang Hao Zhang changxin wu State Key Laboratory of Animal Biotech Breeding Beijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background In poultry, the smooth transition of follicles from the preovulatory-to-postovulatory phase impacts egg production in hens and can benefit the poultry industry. However, the regulatory mechanism underlying follicular ovulation in avians is a complex biological process that remains *** Critical biochemical events involved in ovulation in domestic chickens(Gallus gallus) were evaluated by transcriptomics, proteomics, and in vitro assays. Comparative transcriptome analyses of the largest preovulatory follicle(F1) and postovulatory follicle(POF1) in continuous laying(CL) and intermittent laying(IL) chickens indicated the greatest difference between CL_F1 and IL_F1, with 950 differentially expressed genes(DEGs), and the smallest difference between CL_POF1 and IL_POF1, with 14 DEGs. Additionally, data-independent acquisition proteomics revealed 252 differentially abundant proteins between CL_F1 and IL_F1. Perivitelline membrane synthesis, steroid biosynthesis, lysosomes, and oxidative phosphorylation were identified as pivotal pathways contributing to ovulation regulation. In particular, the regulation of zona pellucida sperm-binding protein 3, plasminogen activator, cathepsin A, and lactate dehydrogenase A(LDHA) was shown to be essential for ovulation. Furthermore, the inhibition of LDHA decreased cell viability and promoted apoptosis of ovarian follicles in *** This study reveals several important biochemical events involved in the process of ovulation, as well as crucial role of LDHA. These findings improve our understanding of ovulation and its regulatory mechanisms in avian species.

关键词： Chicken Data-independent acquisition proteomics LDHA Ovulation Regulatory mechanism Transcriptome

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尖轨端面复杂廓形机器人铣削轨迹规划方法

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中国铁道科学 2024年第3期45卷 12-25页

作者：吴志伟樊文刚李江张峻瑞吴昌昕刘弋北京交通大学机械与电子控制工程学院北京100044 北京交通大学载运工具先进制造与测控技术教育部重点实验室北京100044

铁路道岔尖轨数控成型铣削后的打磨抛光目前普遍由人工完成,存在效率低、质量一致性差和漏打磨等问题,难以满足尖轨高效高质加工需求。而将工业机器人铣削技术应用到打磨抛光工序中虽可提升尖轨制造自动化水平,但尖轨端面廓形的复杂多... 详细信息

铁路道岔尖轨数控成型铣削后的打磨抛光目前普遍由人工完成,存在效率低、质量一致性差和漏打磨等问题,难以满足尖轨高效高质加工需求。而将工业机器人铣削技术应用到打磨抛光工序中虽可提升尖轨制造自动化水平,但尖轨端面廓形的复杂多变以及机器人存在的弱刚性又导致其加工轨迹平滑度难以得到保障。为此,需要对尖轨端面复杂廓形机器人铣削轨迹规划方法进行创新。首先,基于MD-H参数法建立机器人运动学模型并分析其运动特性;然后,采用三次NURBS曲线对尖轨端面廓形进行参数化描述,以铣削作业法向刚度性能指标作为优化目标,建立轨迹点姿态优化模型;同时,考虑机器人关节连续性约束,建立机器人铣削轨迹姿态规划模型;在此基础上,基于S型曲线算法实现机器人铣削速度规划;最后,通过仿真和试验进行验证。结果表明:该方法能够有效控制机器人的运动轨迹,解决了外凸夹角区域的“过切”和“跳刀”问题,加工后尖轨端面廓形更为平滑,能满足尖轨制造要求。

关键词：铁路道岔尖轨工业机器人铣削技术轨迹规划 NURBS曲线

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单孔腹腔镜疝囊高位结扎术治疗小儿腹股沟疝的效果分析

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中国卫生标准管理 2024年第15期15卷 23-26页

作者：陈培君吴长鑫杨甲辰晋江市医院小儿外科福建晋江362200

目的探讨单孔腹腔镜疝囊高位结扎术用于小儿腹股沟疝治疗中的效果及对切口美观度的影响。方法选取2020年9月—2023年7月晋江市医院小儿外科收治的109例小儿腹股沟疝患儿,对其资料行回顾性分析,按照手术方法差异分组,对照组52例采取传统... 详细信息

目的探讨单孔腹腔镜疝囊高位结扎术用于小儿腹股沟疝治疗中的效果及对切口美观度的影响。方法选取2020年9月—2023年7月晋江市医院小儿外科收治的109例小儿腹股沟疝患儿,对其资料行回顾性分析,按照手术方法差异分组,对照组52例采取传统小切口疝囊高位结扎术,观察组57例采取单孔腹腔镜疝囊高位结扎术。比较2组手术效果、切口美观满意度及并发症发生率。结果观察组手术时间、术后肛门排气时间、下床活动时间、住院时间及切口长度短于对照组,观察组术中出血量少于对照组(P<0.05)。观察组术后切口美观满意度为96.49%,明显高于对照组的86.42%(P<0.05)。观察组术后并发症发生率为1.75%,低于对照组的17.31%(P<0.05)。结论单孔腹腔镜疝囊高位结扎术治疗小儿腹股沟疝效果满意,且创伤更小、术后疼痛更轻,术后恢复快,可减轻氧化应激反应及炎症反应,能提高切口美观满意度,也可减少术后并发症发生。

关键词：单孔腹腔镜疝囊高位结扎术小儿腹股沟疝切口美观度并发症

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Expression detection and partial cloning of porcine leptin receptor (OBR) gene

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Chinese Science Bulletin 2001年第5期46卷 396-400页

作者： HU Xiaoxiang DAI Rujuan LI Ming wu changxin College of Animal Science and Technology China Agricultural UniversityBeijing 100094China National Laboratory for Agrobiotechnology China Agricultural UniversityBeijing 100094China

The product of the obesity gene, called leptin, is an important regulator of adiposity, which mainly functions via its regulation of feed intake and energy metabolism. Both obesity gene (ob gene) and leptin receptor gene (OBR gene) are thought to play a major role in controlling of body fat mass as well as body weight. The result of RT-PCR shows that levels of pig OBR mRNA are higher in hypothalamus, lung and liver, and lower expression can be detected in other tissues. Total RNA purified from 11 different organs and tissues have been hybridized with pig OBR cDNA probes. The hybridization signals are shown in 7 organs and tissues. 4.1 and 3.8 kb bands were observed from hypothalamus, whereas 3.8 and 3.5 kb bands were observed in other tissues instead. The nearly complete sequence of the extracellular domain of pig OBR gene was obtained. The homology of sequence is 89.2% between pig and human, 80.3% between pig and mouse. Alignment of the predicted ammo acid sequence of OBR in pig, human and mouse shows

关键词： pig ob (obese) gene OBR (leptin receptor) gene leptin RT-PCR Northern blotting.

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Proteo-transcriptomic profiles reveal key regulatory pathways and functions of LDHA in the ovulation of domestic chickens (Gallus gallus).

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畜牧与生物技术杂志(英文版) 2024年第4期 1449页

作者： Ruixue Nie Wenhui Zhang Haoyu Tian Junying Li Yao Ling Bo Zhang Hao Zhang and changxin wu

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Integrative analysis of miRNA and mRNA profiles reveals that gga-miR-106-5p inhibits adipogenesis by targeting the KLF15 gene in chickens

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1671-1689页

作者： Weihua Tian Xin Hao Ruixue Nie Yao Ling Bo Zhang Hao Zhang changxin wu National Engineering Laboratory for Animal Breeding Beijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Sanya Institute of China Agricultural University Hainan 572025 SanyaChina.

Background:Excessive abdominal fat deposition in commercial broilers presents an obstacle to profitable meat quality,feed utilization,and *** fat deposition depends on the proliferation of preadipocytes and their maturation into adipocytes,which involves a cascade of regulatory *** evidence has shown that microRNAs(miRNAs)serve as post-transcriptional regulators of adipogenic differentiation in ***,the miRNA-mediated molecular mechanisms underlying abdominal fat deposition in chickens are still poorly *** study aimed to investigate the biological functions and regulatory mechanism of miRNAs in chicken abdominal ***:We established a chicken model of abdominal adipocyte differentiation and analyzed miRNA and mRNA expression in abdominal adipocytes at different stages of differentiation(0,12,48,72,and 120 h).A total of 217 differentially expressed miRNAs(DE-miRNAs)and 3520 differentially expressed genes were *** prediction of DE-miRNAs and functional enrichment analysis revealed that the differentially expressed targets were significantly enriched in lipid metabolism-related signaling pathways,including the PPAR signaling and MAPK signaling pathways.A candidate miRNA,gga-miR-106-5p,exhibited decreased expression during the proliferation and differentiation of abdominal preadipocytes and was downregulated in the abdominal adipose tissues of fat chickens compared to that of lean ***-miR-106-5p was found to inhibit the proliferation and adipogenic differentiation of chicken abdominal preadipocytes.A dual-luciferase reporter assay suggested that the KLF15 gene,which encodes a transcriptional factor,is a direct target of ***-miR-106-5p suppressed the posttranscriptional activity of KLF15,which is an activator of abdominal preadipocyte proliferation and differentiation,as determined with gain-and loss-of-function ***:gga-miR-106-5p function

关键词： Abdominal fat Adipogenesis Chickens gga-miR-106-5p KLF15 MiRNA

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CircDOCK7 facilitates the proliferation and adipogenic differentiation of chicken abdominal preadipocytes through the gga‑miR‑301b‑3p/ACSL1 axis

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2290-2315页

作者： Weihua Tian Ye Liu Wenhui Zhang Ruixue Nie Yao Ling Bo Zhang Hao Zhang changxin wu National Engineering Laboratory for Animal Breeding Beijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background Abdominal fat deposition depends on both the proliferation of preadipocytes and their maturation into adipocytes,which is a well-orchestrated multistep process involving many regulatory *** RNAs(circRNAs)have emergingly been implicated in mammalian ***,circRNA-mediated regulation in chicken adipogenesis remains *** previous circRNA sequencing data identified a differentially expressed novel circRNA,8:27,886,180|27,889,657,during the adipogenic differentiation of chicken abdominal *** study aimed to investigate the regulatory role of circDOCK7 in the proliferation and adipogenic differentiation of chicken abdominal preadipocytes,and explore its molecular mechanisms of competing endogenous RNA underlying chicken *** Our results showed that 8:27,886,180|27,889,657 is an exonic circRNA derived from the head-to-tail splicing of exons 19–22 of the dedicator of cytokinesis 7(DOCK7)gene,abbreviated as ***7 is mainly distributed in the cytoplasm of chicken abdominal preadipocytes and is stable because of its RNase R resistance and longer ***7 is significantly upregulated in the abdominal fat tissues of fat chickens compared to lean chickens,and its expression gradually increases during the proliferation and adipogenic differentiation of chicken abdominal ***,the gain-and loss-of-function experiments showed that circDOCK7 promoted proliferation,G0/G1-to S-phase progression,and glucose uptake capacity of chicken abdominal preadipocytes,in parallel with adipogenic differentiation characterized by remarkably increased intracellular lipid droplet accumulation and triglyceride and acetyl coenzyme A content in differentiated chicken abdominal ***,a pull-down assay and a dual-luciferase reporter assay confirmed that circDOCK7 interacted with gga-miR-301b-3p,which was identified as an inhibitor of chicken abdominal adipogene

关键词： Abdominal fat deposition Adipogenesis Chickens CircDOCK7 Competing endogenous RNA MiRNA sponge

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The combined genotypes effect of ESR and FSHβ genes on litter size traits in five different pig breeds

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Chinese Science Bulletin 2001年第2期46卷 140-143页

作者： Kefei Chen Ning Li Lusheng Huang Qin Zhang Jiansheng Zhang Shiquan Sun Ming Luo changxin wu Provincial Key Laboratory for Animal Biotechnology Jiangxi Agricultural University Nanchang China National Key Laboratory for Agrobiotechnology China Agricultural University Beijing China Changshu Livestock Breeding Experimental Farm Changshu China

Estrogen receptor (ESR) and Follicular-stimula-ting hormone beta subunit (FSHβ) genes were chosen as candidates to determine whether they control litter size and some other reproductive traits in swine. 269 sows from five different pig breeds were genotyped by an established PCR-RFLPs protocol at both ESR and FSHβ loci. The effects of both ESR and FSHβ on pig reproductive traits, including total number born (TNB) and number born alive (NBA), are analyzed by SAS software (version 6.12). These computation results demonstrated that both ESR locus and FSHβ locus are the major genes influencing litter size in pigs. The sows of BBBB combined genotype of ESR and FSHβ loci generally produce 1.85-3.01 TNB and 2.0-3.0 NBA more than those of ABAA combined genotype. The notable effect of ESR locus and FSHβ locus on litter size of pigs have made it possible to improve the pig reproduction by Marker-assisted selection (MAS). Moreover, introgression of the beneficial alleles into commercial pig breeding lines,

关键词： ESR FSHβ porcine combined genotypes litter size.

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Semantic segmentation via pixel-to-center similarity calculation

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CAAI Transactions on Intelligence Technology 2024年第1期9卷 87-100页

作者： Dongyue wu Zilin Guo Aoyan Li Changqian Yu Nong Sang changxin Gao National Key Laboratory of Science and Technology on Multispectral Information Processing School of Artificial Intelligence and AutomationHuazhong University of Science and TechnologyWuhanChina Meituan Inc. BeijingChina

Since the fully convolutional network has achieved great success in semantic segmentation,lots of works have been proposed to extract discriminative pixel ***,the authors observe that existing methods still suffer from two typical challenges:(i)The intra-class feature variation between different scenes may be large,leading to the difficulty in maintaining the consistency between same-class pixels from different scenes;(ii)The inter-class feature distinction in the same scene could be small,resulting in the limited performance to distinguish different classes in each *** authors first rethink se-mantic segmentation from a perspective of similarity between pixels and class *** weight vector of the segmentation head represents its corresponding semantic class in the whole dataset,which can be regarded as the embedding of the class ***,the pixel-wise classification amounts to computing similarity in the final feature space between pixels and the class *** this novel view,the authors propose a Class Center Similarity(CCS)layer to address the above-mentioned challenges by generating adaptive class centers conditioned on each scenes and supervising the similarities between class *** CCS layer utilises the Adaptive Class Center Module to generate class centers conditioned on each scene,which adapt the large intra-class variation between different *** designed Class Distance Loss(CD Loss)is introduced to control both inter-class and intra-class distances based on the predicted center-to-center and pixel-to-center ***,the CCS layer outputs the processed pixel-to-center similarity as the segmentation *** experiments demonstrate that our model performs favourably against the state-of-the-art methods.

关键词： computer vision deep neural networks image segmentation scene understanding

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Cloned pigs derived from somatic cell nuclear transferembryos cultured in vitro atlow oxygen tension

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Chinese Science Bulletin 2006年第7期51卷 839-844页

作者： PAN Dengke ZHANG Yunhai SUN Xiuzhu ZHANG Jian LI Xuyang LI Yan GU Zhiliang DA Yunping wu changxin LI Ning State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China College of Animal Science and Technology China Agricultural Uni versity Beijing 100094 China Animal Clinical Hospital China Agricultural University Beijing 100094 China Hebei Minghui Group of Pigs Breeding Sanhe 065200 China

Pig cloning has great potential to human xenotransplantation. The present study was designed to establish a more efficient system for producing cloned pigs by somatic cell nuclear transfer (SCNT). Our approach was as follows: SCNT embryos were reconstructed by using fetal fibroblasts of Chinese miniature pig as donors and in vitro matured oocytes of prepubertal gilts as recipients. Reconstructed em- bryos were induced by electrical fusion/activation and cultured in BSA-containing North Carolina State University 23 medium (NCSU-23) or Porcine Zygote Medium (PZM-3) at the gas condition of 5% CO2, 7% O2, 88% N2. A total of 230 cloned embryos were transferred to three surrogate sows, producing three piglets. One of them is apparently healthy. The clonal provenance of the piglet was indicated by its coat color and confirmed by DNA microsatellite analysis. These results indicate that the use of in vitro matured oocytes from prepubertal gilts as recipient, combined with cloned embryos cultured at low oxygen tension is an effective way to produce cloned pigs.

关键词：卵母细胞体外发育无性繁殖猪体细胞克隆迁移晶胚

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