Cloned pigs derived from somatic cell nuclear transferembryos cultured in vitro atlow oxygen tension

作     者：PAN Dengke ZHANG Yunhai SUN Xiuzhu ZHANG Jian LI Xuyang LI Yan GU Zhiliang DA Yunping WU Changxin LI Ning 

作者机构：State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China College of Animal Science and Technology China Agricultural Uni versity Beijing 100094 China Animal Clinical Hospital China Agricultural University Beijing 100094 China Hebei Minghui Group of Pigs Breeding Sanhe 065200 China 

出 版 物：《Chinese Science Bulletin》 (Chin. Sci. Bull.)

年 卷 期：2006年第51卷第7期

页      面：839-844页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported in part by the Major State Basic Research Development Program of China(Grant No.G20000161) State High-tech Research Development Program(Grant No.2003AA205102) the Natural Science Foundation of Beijing(Grant No.5030001) 

主　　题：卵母细胞 体外发育 无性繁殖 猪 体细胞克隆 迁移晶胚 

摘      要：Pig cloning has great potential to human xenotransplantation. The present study was designed to establish a more efficient system for producing cloned pigs by somatic cell nuclear transfer (SCNT). Our approach was as follows: SCNT embryos were reconstructed by using fetal fibroblasts of Chinese miniature pig as donors and in vitro matured oocytes of prepubertal gilts as recipients. Reconstructed em- bryos were induced by electrical fusion/activation and cultured in BSA-containing North Carolina State University 23 medium (NCSU-23) or Porcine Zygote Medium (PZM-3) at the gas condition of 5% CO2, 7% O2, 88% N2. A total of 230 cloned embryos were transferred to three surrogate sows, producing three piglets. One of them is apparently healthy. The clonal provenance of the piglet was indicated by its coat color and confirmed by DNA microsatellite analysis. These results indicate that the use of in vitro matured oocytes from prepubertal gilts as recipient, combined with cloned embryos cultured at low oxygen tension is an effective way to produce cloned pigs.