Expression detection and partial cloning of porcine leptin receptor (OBR) gene

作     者：HU Xiaoxiang DAI Rujuan LI Ming WU Changxin 

作者机构：College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100094China National Laboratory for AgrobiotechnologyChina Agricultural UniversityBeijing 100094China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2001年第46卷第5期

页      面：396-400页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：the National Natural Science Foundation of China (Grant No. 39725022 ) the State Key Basic Research Development Program (Grant No. 2000016100) 

主　　题：pig ob (obese) gene OBR (leptin receptor) gene leptin RT-PCR Northern blotting. 

摘      要：The product of the obesity gene, called leptin, is an important regulator of adiposity, which mainly functions via its regulation of feed intake and energy metabolism. Both obesity gene (ob gene) and leptin receptor gene (OBR gene) are thought to play a major role in controlling of body fat mass as well as body weight. The result of RT-PCR shows that levels of pig OBR mRNA are higher in hypothalamus, lung and liver, and lower expression can be detected in other tissues. Total RNA purified from 11 different organs and tissues have been hybridized with pig OBR cDNA probes. The hybridization signals are shown in 7 organs and tissues. 4.1 and 3.8 kb bands were observed from hypothalamus, whereas 3.8 and 3.5 kb bands were observed in other tissues instead. The nearly complete sequence of the extracellular domain of pig OBR gene was obtained. The homology of sequence is 89.2% between pig and human, 80.3% between pig and mouse. Alignment of the predicted ammo acid sequence of OBR in pig, human and mouse shows