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Asian Journal of Andrology 2012年第2期14卷 294-300页

作者： Qiao-Xia Zhang Xiao-Yan Zhang Zhen-Ming Zhang Wei Lu Ling Liu Gang Li Zhi-Ming Cai Yao-Ting Gui chawnshang chang The Guangdong and Shenzhen Key Lab of Male Reproductive Medicine and Genetics Sex Hormone Research CenterPeking University Shenzhen HospitalShenzhen PKU-HKUST Medical CenterShenzhen 518036China Department of Biochemistry and Molecular Biology Peking University Health Science CenterPeking UniversityBeijing 100083China George H.Whipple Lab for Cancer Research Departments of Pathology and UrologyUniversity of Rochester Medical CenterRochester NY 14642USA Sex hormone Research Center China Medical UniversityTaichung 404TaiwanChina

Androgen and androgen receptor （AR） play important roles in male spermatogenesis and fertility, yet detailed androgen/AR signals in Sertoli cells remain unclear. To identify AR target genes in Sertoli cells, we analyzed the gene expression profiles of testis between mice lacking AR in Sertoli cells （S-AR-/y） and their littermate wild-type （WT） mice. Digital gene expression analysis identified 2276 genes downregulated and 2865 genes upregulated in the S-AR-/y mice testis compared to WT ones. To further nail down the difference within Sertoli cells, we first constructed Sertoli cell line TM4 with stably transfected AR （named as TM4/AR） and found androgens failed to transactivate AR in Sertoli TM4 and TM4/AR cells. Interestingly, additional transient transfection of AR-cDNA resulted in significant androgen responsiveness with TM4/AR cells showing 10 times more androgen sensitivity than TM4 cells. In the condition where maximal androgen response was demonstrated, we then analyzed gene expression and found the expression levels of 2313 genes were changed more than twofold by transient transfection of AR-cDNA in the presence of testosterone. Among these genes, 603 androgen-/ AR-regulated genes, including 164 upregulated and 439 downregulated, were found in both S-AR-/y mice testis and TM4/AR cells. Using informatics analysis, the gene ontology was applied to analyze these androgen-/AR-regulated genes to predict the potential roles of androgen/AR in the process of spermatogenesis. Together, using gene analysis in both S-AR-/y mice testis and TM4/AR cells may help us to better understand the androeen/AR signals in Sertoli cells and their influences in spermatogenesis.

关键词： androgen receptor conditional knockout mice Sertoli cells testis TM4 cells

Expression of human AR cDNA driven by its own promoter results in mild promotion, but not suppression, of growth in human prostate cancer PC-3 cells

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Asian Journal of Andrology 2007年第2期9卷 181-188页

作者： Saleh Altuwaijri Cheng-Chia Wu Yuan-Jie Niu Atsushi Mizokami Hong-Chiang chang chawnshang chang Departments of Pathology and Urology and the Cancer Center University of Rochester Medical Center Rochester New York 14642 USA King Faisal Specialist Hospital and Research Center Riyadh 11211 Saudi Arabia Department of Urology Karazawu University Karazawu City Japan Department of Urology National Taiwan University Taipei Taiwan China

Aim： To examine the physiological role of the androgen receptor （AR） in the PC-3 cell line by transfecting full-length functional AR cDNA driven by its natural human AR promoter. Methods： We generated an AR-expressing PC-3（AR）9 stable clone that expresses AR under the control of the natural human AR promoter and compared its proliferation to that of the PC-3（AR）2 （stable clone that expresses AR under the control of the cytomegalovirus （CMV） promoter, established by Heisler et al.） after androgen treatment. Results： We found that dihydrotestosterone （DHT） from 0.001 nmol/L to 10 nmol/L induces cell cycle arrest or inhibits proliferation of PC-3（AR）2 compared with its vector control, PC-3（plRES）. In contrast, PC-3（AR）9 cell growth slightly increased or did not change when treated with physiological concentrations of 1 nmol/L DHT. Conclusion： These data suggest that intracellular control of AR expression levels through the natural AR promoter might be needed for determining AR function in androgen-independent prostate cancer （AIPC） PC-3 cells. Unlike previous publications that showed DHT mediated suppression of PC-3 growth after transfection of viral promoter-driven AR overexpression, we report here that DHT-mediated PC-3 proliferation is slightly induced or does not change compared with its baseline after reintroducing AR expression driven by its own natural promoter, as shown in PC-3（AR）9 prostate cancer cells.

关键词： androgen receptor proliferation apoptosis prostate cancer androgen ablation therapy

Differential regulation of glutathione S-transferase Yb1mRNA levels in rat prostate, liver and brain by androgen

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Cell Research 1995年第2期5卷 235-242页

作者： ZHANG YUAN YOUDUAN ZHANG chawnshang chang YONGLIAN ZHANG(Shanghai Institute of Biochemistry, Academia Sinica, 320Yueyang Road, Shanghai 200031, China.)(Department of Human Oncology, University of Wisconsin, 600 Highland Avenue, Madison, WI 53792, USA.) Shanghai Institute of Biochemistry Academia Sinica Shanghai China Department of Human Oncology University of Wiscon-sin USA

Northern blot analysis of glutathione S-transferase (GST) Yb1 mRNA in different tissues of male and female rats revealed that its tissue-specific transcription patterns were highly sex hormone related. Although the GST Yb1 mRNA could be detected in most of the tissues examined at various levels, the highest abundance was observed in the ventral prostate, uterus and liver, which were the main target tissue for androgen, estrogen and glucocorticoid respectively The effect of androgen on the transcription of GST Yb1 was also tissue-specific. Since androgen withdrawal by castration caused the up-regulation of GST Yb1 mRNA in the ventral prostate but down-regulation in the liver and no effect in the brain, evaluation of this system for studying the regulation mechanisms of gene expression by which androgen exerts its differential effects has been discussed.

关键词： GST Yb1 mRNA androgen regulation rat prostate rat liver rat brain

Loss of Epithelial Androgen Receptor Promotes Prostate Cancer Progression

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Loss of Epithelial Androgen Receptor Promotes Prostate Cance...

The diverse and contrasting effects of using human prostate cancer cell lines to study androgen receptor roles in prostate cancer

第十五届全国泌尿外科学术会议

作者：牛远杰 Shuyuan Yeh chawnshang chang Tianjin Institute of Urological Surgery Tianjin Medical University George Whipple Laboratory for Cancer Research University of Rochester Rochester

Using tissue recombination of human prostatic cancer epithelial cell lines (PC3-v or PC3-AR9) with human prostatic stromal cell lines (WPMY1-v or WPMY1-ARsi), we demonstrated that the AR might function as a suppressor...

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Asian Journal of Andrology 2009年第1期11卷 39-48页

作者： Sheng-Qiang Yu Kuo-Pao Lai Shu-Jie Xia Hong-Chiang chang chawnshang chang Shuyuan Yeh Department of Urology The First People＇s Hospital of Shanghai Jiao Tong University Shanghai 200080 China George Whipple Laboratory for Cancer Research Departments of Pathology and Urology The Cancer Center Universityof Rochester Rochester NY 14642 USA Department of Urology National Taiwan University Hospital Taipei 100 Taiwan China

The androgen receptor （AR） plays an important role in the development and progression of prostate cancer （PCa）. Androgen deprivation therapy is initially effective in blocking tumor growth, but it eventually leads to the hormonerefractory state. The detailed mechanisms of the conversion from androgen dependence to androgen independence remain unclear. Several PCa cell lines were established to study the role of AR in PCa, but the results were often inconsistent or contrasting in different cell lines, or in the same cell line grown under different conditions. The cellular and molecular alteration of epithelial cells and their microenvironments are complicated, and it is difficult to use a single cell line to address this important issue and also to study the pathophysiological effects of AR. In this paper, we summarize the different effects of AR on multiple cell lines and show the disadvantages of using a single human PCa cell line to study AR effects on PCa. We also discuss the advantages of widely used epithelium-stroma co-culture systems, xenograft mouse models, and genetically engineered PCa mouse models. The combination of in vitro cell line studies and in vivo mouse models might lead to more credible results and better strategies for the study of AR roles in PCa.

关键词： androgen receptor cell lines epithelium-stroma co-culture mouse models prostate cancer

Recent advances in the study of testicular nuclear receptor 4

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第3期14卷 171-177页

作者： Xian-fan DING Shi-cheng YU Bi-de CHEN Shin-jen LIN chawnshang chang Gong-hui LI Department of Urology Sir Run Run Shaw HospitalSchool of MedicineZhejiang University George Whipple Lab for Cancer Research Departments of Pathology and UrologyUniversity of Rochester

Testicular nuclear receptor 4(TR4),also known as NR2C2(nuclear receptor subfamily 2,group C,member 2),is a transcriptional factor and a member of the nuclear receptor ***4 was initially cloned from human and rat hypothalamus,prostate,and testes *** almost two decades,its specific tissue distribution,genomic organization,and chromosomal assignment have been well investigated in humans and ***,it has been very difficult to study TR4's physiological functions due to a lack of specific *** knock-out animal techniques provide an alternative approach for defining the biological functions of *** vivo studies of TR4 gene knockout mice(TR4-/-) found that they display severe spinal curvature,subfertility,premature aging,and prostate prostatic intraepithelial neoplasia(PIN) *** modulators,downstream target gene regulation,feedback mechanisms,and differential modulation mediated by the recruitment of other nuclear receptors and coregulators have been identified in studies using the TR4-/-*** the establishment of a tissue-specific TR4-/-mouse model,research on TR4 will be more convenient in the future.

关键词：核受体睾丸前列腺癌组织分布动物技术基因敲除小鼠模型染色体分配

Corrigendum to‘New therapy targeting differential androgen receptor signaling in prostate cancer stem/progenitor ***-stem/progenitor cells’

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Journal of Molecular Cell Biology 2020年第12期12卷 991-992页

作者： Soo Ok Lee Zhifang Ma Chiuan-Ren Yeh Jie Luo Tzu-Hua Lin Kuo-Pao Lai Shinichi Yam ashita Liang Liang Jing Tian Lei Li Qi Jiang Chiung-Kuei Huang Yuanjie Niu Shuyuan Yeh and chawnshang chang George Whipple Lab for Cancer Research Department of PathologyUniversity of Rochester Medical CenterRochesterNY 14642USA George Whipple Lab for Cancer Research Department of UrologyUniversity of Rochester Medical CenterRochesterNY 14642USA George W hipple Lab for Cancer Research Department of Radiation OncologyUniversity of Rochester Medical CenterRochesterNY 14642USA George Whipple Lab for Cancer Research The Wilmot Cancer CenterUniversity of Rochester Medical CenterRochesterNY 14642USA Department of Urology First Hospital of Shanxi Medical UniversityTaiyuan 030001China Sex Hormone Research Center Department of UrologyThe First Affiliated HospitalXi an Jiaotong UniversityXi an 710061China chawnshang chang Sex Hormone Research Center Tianjin Institute of UrologyTianjin Medical UniversityTianjin 300211China Sex Hormone Research Center China Medical University and HospitalTaichung A04

In this article,there were errors in the images presented in Figure 3Ab(bottom left)and Figure 5D(2nd and 4th columns).These were caused by miss-insertion of the images during preparation of the manuscript for *** raw data of the images in Figure 5D were lost,the authors re-performed the whole experiment and provided new similar results(both images and quantitation graphs)for an *** corrected Figure 3Ab left panel and updated Figure 5D are shown as *** details have been corrected only in this corrigendum to preserve the published version of *** authors assure that the original results or conclusions are not altered and they apologize for the mistake.

关键词： Figure corrected details

Androgen Receptor Is a Tumor Suppressor and Proliferator in Prostate Cancer

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中国肺癌杂志 2009年第6期12卷 I0022-I0022页

作者： chawnshang chang Tianjin Institute of Urological Surgery Tianjin Medical University

关键词： ADT 药物抑制剂肺癌化疗疗效

Tissue PSA facilitates refractory prostate tumor progression via enhancing ARA70-induced androgen receptor transactivation

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Tissue PSA facilitates refractory prostate tumor progression...