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Injection of bone marrow mesenchymal stem cells by intravenous or intraperitoneal routes is a viable alternative to spinal cord injury treatment in mice

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Neural Regeneration Research 2018年第6期13卷 1046-1053页

作者： bruna dos santos Ramalho FernANDa Martins de Almeida Conrado Mendonca Sales Silmara de Lima Ana Maria Blanco Martinez Laboratorio de Neurodegeneracao e Reparo Departamento de Patologia - Faculdade de Medicina HUCFF Universidade Federal do Rio de Janeiro Rio de laneiro Brazil

In spite of advances in surgical care and rehabilitation, the consequences of spinal cord injury （SCI） are still challenging. Several experimental therapeutic strategies have been studied in the SCI field, and recent advances have led to the development of therapies that may act on the inhibitory microenvironment. Assorted lineages of stem cells are considered a good treatment for SCI. This study investigated the effect of systemic transplantation of mesenchymal stem cells （MSCs） in a compressive SCI model. Here we present results of the intraperitoneal route, which has not been used previously for MSC administration after compressive SCI. We used adult female C57BL/6 mice that underwent laminectomy at the T9 level, followed by spinal cord compression for 1 minute with a 30-g vascular clip. The animals were divided into five groups： sham （anesthesia and laminectomy but without compression injury induction）, MSC i.p. （intraperitoneal injection of 8×10^5 MSCs in 500 μL of DMEM at 7 days after SCI）, MSC i.v. （intravenous injection of 8 × 10^5 MSCs in 500μL of DMEM at 7 days after SCI）, DMEM i.p. （intraperitoneal injection of 500μL of DMEM at 7 days after SCI）, DMEM i.v. （intravenous injection of 500 μL of DMEM at 7 days after SCI）. The effects of MSCs transplantation in white matter sparing were analyzed by luxol fast blue staining. The number of preserved fibers was counted in semithin sections stained with toluidine blue and the presence of trophic factors was analyzed by immunohistochemistry. In addition, we analyzed the locomotor performance with Basso Mouse Scale and Global Mobility Test. Our results showed white matter preservation and a larger number of preserved fibers in the MSC groups than in the DMEM groups. Furthermore, the MSC groups had higher levels of trophic factors （brain-derived neurotrophic factor, nerve growth factor, neurotrophin-3 and neurotrophin-4） in the spinal cord and improved locomotor performance. Our results indicate that injection of MSC

关键词： spinal cord injury mesenchymal stem cells intravenous route intraperitoneal route functional recovery systemic transplantation neural regeneration

Optimizing the evolution of strength for lime-stabilized rammed soil

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Journal of Rock Mechanics and Geotechnical Engineering 2019年第4期11卷 882-891页

作者： Jair de jesus Arrieta Baldovino Ronaldo Luis dos santos Izzo Eclesielter Batista Moreira Juliana Lundgren Rose department of Civil Construction Federal University of Technology-Parana

In the present study,unconfined compressive strength(qu)values of two lime-treated soils(soil 1 and 2)with curing times of 28 d,90 d and 360 d were *** influence of void/lime ratio was represented by the porosity/volumetric lime content ratio(η/Liv)as the main parameter.η/Liv represents the volume of void influenced by compaction effort and lime *** evolution of qu was analyzed for each soil using the coefficient of determination as the optimization *** at providing adjustments to the mechanical resistance values,the η/Liv parameter was modified to η/LivC using the adjustment exponent C(to make qu-η/Liv variation rates compatible).The results show that with the decrease of η/*** increases potentially and the optimized values of C were *** mechanical resistance data show similar trends between qu and η/LivC for the studied silty soil-ground lime mixtures,which were cured at ambient temperature(23±2)℃ with different curing times of 28-360 ***,optimized equations were presented using the normalized strengths and the proposed optimization model,which show 6% error and 95% acceptability on average.

关键词： Lime-treated soil Void/lime ratio Ground improvement

Real drive cycles analysis by ordered power methodology applied to fuel consumption,C0_(2),NO_(x) and PM emissions estimation

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Frontiers of Environmental Science & Engineering 2021年第1期15卷 77-90页

作者： Pol Masclans Abello Vicente Medina Iglesias M.Antonia de los santos Lopez jesus Alvarez-Florez Center for Engines and Heat Installation Research(CREMIT-UPC).Universitat Politecnica de Catalunya-BarcelonaTech.Av.Diagonal 547(ETSEIB)08028 Barcelona Spain department of Thermal Machinery of Universitat Politecnica de Catalunya-BarcelonaTech and CREMIT-UPC.-BarcelonaTech.Av.Diagonal 547(ETSEIB)08028 Barcelona Spain 3 department of Mechanical Engineering of Universitat Politecnica de Catalunya-BarcelonaTech and CREMIT-UPC.-BarcelonaTech.Av.Diagonal 547(ETSEIB)08028 Barcelona Spain

In this work three fuel consumption and exhaust emission models,ADVISOR,VT-MICRO and the European Environmental Agency Emission factors,have been used to obtain fuel consumption(FC)and exhaust *** models have been used at micro-scale,using the two signal treatment methods *** manuscript presents:1)a methodology to collect data in real urban driving cycles,2)an estimation of FC and tailpipe emissions using some available models in literature,and 3)a novel analysis of the results based on delivered wheel *** results include Fuel Consumption(FC),CO_(2),NO_(x) and PM_(10) emissions,which are derived from the three *** the first part of the paper we present a new procedure for incomplete drive cycle data treatment,which is necessary for real drive cycle acquisition in high density *** the models are used to obtain second by second FC and exhaust ***,a new methodology named Cycle Analysis by Ordered Power(CAbOP)is presented and used to compare the *** method consists in the re-ordering of time dependant data,considering the wheel mechanical power domain instead of the standard time *** new strategy allows the 5 situations in drive cycles to be clearly visualized:hard breaking zone,slowdowns,idle or stop zone,sustained speed zone and acceleration *** complete methodology is applied in two real drive cycles surveyed in Barcelona(Spain)and the results are compared with a standardized WLTC urban cycle.

关键词： Cycle Analysis by Ordered Power(CAbOP) Micro and macro models Real drive cycle NO_(x)/PM_(10)/CO_(2)emissions Wheel mechanical power domain Worldwide Harmonized Light-Duty Vehicles Test Cycle(WLTC)

Hydrological variability and long-term floristic-structural modifications in different habitats of a tropical semi-deciduous forest

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Journal of Forestry Research 2022年第3期33卷 801-811页

作者： Alisson Borges Miranda santos Vinicius Andrade Maia Cléber Rodrigo de Souza Natália de Aguiar-Campos Aurélio de jesus Rodrigues Pais Wilder Bento da Silva Nathalle Cristine Alencar Fagundes Jean Daniel Morel Rubens Manoel dos santos department of Forestry Sciences Federal University of LavrasCampus UniversitárioC.P.3037LavrasBrazil Faculty of Environmental Engineering and Natural Resources Zambeze UniversityChimoioC.P.2400Mozambique Biological Sciences Universidade do Estado de Minas GeraisUnidade ItuiutabaAv.Antônio Carlos 6627Ituiutaba31270-901Brazil department of Biology Federal University of LavrasUniversity campusC.P.3037Lavras37200-000Brazil

Shifts in hydrological regimes alter river flow rates and flood pulses, decrease environmental heterogeneity and the floristic-structural complexity of associated plant communities. We tested the hypothesis that drought events affect plant community composition and structure at a small-scale within a riparian fragment towards a reduction in floristic-structural complexity. The tree community was sampled in three habitats (wet, transitional and dry) and monitored in seven inventories carried out between 1991 and 2018. Hydrological variations were evaluated through annual rainfalls, river flow rates and water level data. The species richness and the detrended correspondence analysis axes were used to characterise the temporal modifications in floristic composition. Community structure was described in terms of biomass: accumulated, growth of survivors, mortality and recruitment. Generalised linear mixed models were fitted to evaluate the effects of time and environment in community. It was concluded that the climate has become drier in recent years due to declining precipitation that has affected flow rates and water levels. The floristic-structural complexity of the study fragment was maintained during the monitoring period. However, prolonged and extreme drought events displayed the potential to impact floristic-structural patterns.

关键词： Forest dynamics Climate changes Semi-deciduous seasonal forest Temporal change Functioning communities

Blinding Lights: the Creative Queer Geography of Daniel Nolasco’s Dry Wind

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Journal of Literature and Art Studies 2021年第5期11卷 345-350页

作者： Diego santos Vieira de jesus ESPM-Rio Rio de JaneiroBrazil

The aim of this paper is to examine the creative queer geography of Daniel Nolasco’s movie Dry *** main argument indicates that,instead of representing countryside cities exclusively as places of repression and denial,the movie shows part of the countryside gay *** realism of the fertilizer factory and the aridity of the city of Catalão are opposed to the extreme stylization of vibrant colors and blinding neon lights of the main character’s fetishist imagination,which indicates how he increasingly surrenders to his *** the pornographic representation of homoerotic desire,Dry Wind also proposes alternatives for affective expressions of masculinities that do not conform to the prevailing moral standards.

关键词： Dry Wind Daniel Nolasco queer cinema queer geography fetishism homoerotism masculinity

Like a Wrecking Ball: The Creative Queer Spaces of Gustavo Vinagre’s “New Dubai”

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Journal of Literature and Art Studies 2020年第12期10卷 1142-1147页

作者： Diego santos Vieira de jesus ESPM-Rio Rio de JaneiroBrazil

The aim of the article is to analyse the creative queer spaces of Gustavo Vinagre’s movie New Dubai. The mainargument indicates that gay sex creatively brings the possibility of resistance against gentrification and questionsheteronormative rules in the organization of society and space. Sex and the violence of the interventions in thecontext of the real estate speculation point to the violence and voyeurism of filming, especially of filming sex.

关键词： Queer spaces Queer movies Gustavo Vinagre New Dubai Brazilian cinema Brazil gentrification

Three Tales of Capitalism and Creative Economy

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International Relations and Diplomacy 2020年第2期8卷 39-44页

作者： Diego santos Vieira de jesus ESPM-Rio Rio de JaneiroBrazil

The aim is to examine how the creative economy develops in shareholder,state,and stakeholder *** the shareholder capitalism,the creative economy is headed by large companies in cooperation with governments,which replicate pregiven formulas of governance with no consideration of local specificities,and reinforces the exclusion of small and medium-sized creative *** state capitalism has the government authorities as the main drivers of the creative economy’s growth,which is usually connected to their political interest in preserving authority and manipulating the *** the stakeholder capitalism,companies should pay their fair share of taxes,show zero tolerance for corruption,uphold human rights throughout their global supply chains,and advocate for a competitive level playing *** elements create the specific conditions for a more diversified set of actors leading the initiatives dedicated to the development of creative economy.

关键词： creative economy shareholder capitalism state capitalism stakeholder capitalism

Beyond the Playboy and the Hero: The (Re)constructions of Roberto Carlos’s work in “The Songs You Danced for Me”

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Journal of Literature and Art Studies 2020年第2期10卷 146-152页

作者： Diego santos Vieira de jesus ESPM-Rio Rio de JaneiroBrazil

The aim is to examine how the musical work of Roberto Carlos was mobilized in the danceshow“The songs you danced for me”.The contact between the songs and the contemporary dance defined different *** results indicate that such contact reaffirms romantic love and the heroism of the protagonist,but also opens possibilities for relationships that escape from the heteronormative romantic ideal.

关键词： Roberto Carlos The songs you danced for me contemporary dance Brazilian music Brazilian dance Jovem Guarda

The Rebel Madame: Madonna’s Postmodern Revolution

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Journal of Literature and Art Studies 2021年第1期11卷 26-32页

作者： Diego santos Vieira de jesus ESPM-Rio Rio de JaneiroBrazil

The aim is to examine Madonna’s revolution regarding gender,sexuality,politics,and religion with the focus on her songs,videos,and live *** main argument indicates that Madonna has used postmodern strategies of representation to challenge the foundational truths of sex and gender,promote gender deconstruction and sexual multiplicity,create political sites of resistance,question the Catholic dissociation between the physical and the divine,and bring visual and musical influences from multiple cultures and marginalized identities.

关键词： Madonna postmodernism pop culture sex gender sexuality politics religion spirituality