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袖珍设备电池稳压器设计考虑

电子设计技术 1994年第10期 39-40+43-45页

作者： bruce d.Moore

使用四节硷性AA电池构成稳压器作为袖珍电子设备的电源,不仅应该能调节,而且也具备效率高、补偿电流和体积小等功能, 从而引出了各种稳压器结构的设计,供设计人员选择。

关键词：稳压器四节电源输入电压电池组峰值电流

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对硫磷纳米抗体筛选及分子识别机制研究

分析化学 2019年第9期47卷 1419-1428页

作者：张玉琪张瑾如王锋李家冬司睿吕丽珊沈玉栋王弘 HAMMOCk bruce d.孙远明华南农业大学食品学院广东省食品质量与安全重点实验室畜禽产品精准加工与安全控制技术国家地方联合工程研究中心(广东) 广州安诺科技股份有限公司 d.partment of Entomology and.UCd.Comprehensive Cancer Center University of CaliforniaDavis

对硫磷是一种广谱的有机磷酸酯类杀虫、杀螨剂,毒性极强,可对环境和人类健康造成严重危害。尽管我国已禁止使用对硫磷,但仍存在违禁使用现象,加强对其监测十分必要。纳米抗体是已知最小的与抗原结合的片段（15 kd.）,来源于骆驼科动物... 详细信息

对硫磷是一种广谱的有机磷酸酯类杀虫、杀螨剂,毒性极强,可对环境和人类健康造成严重危害。尽管我国已禁止使用对硫磷,但仍存在违禁使用现象,加强对其监测十分必要。纳米抗体是已知最小的与抗原结合的片段（15 kd.）,来源于骆驼科动物体内重链抗体的可变区,具有稳定性强、灵敏度高、易表达、水溶性好等特性。本研究基于噬菌体展示技术构建了抗对硫磷纳米抗体库,其库容为2.41×10;cfu/mL,经抗原-抗体固相亲和筛选及噬菌体酶联免疫法（Phage ELISA）鉴定,获得5种抗对硫磷纳米抗体VHH1、VHH6、VHH13、VHH21和VHH24。以结合能力最强的VHH1为基础,采用同源建模和分子对接技术探讨VHH1与对硫磷分子的识别机制,发现VHH1与对硫磷存在氢键和疏水间分子作用力,关键氨基酸是Ser60、Lys104、Phe105、Gly106和Arg107。本研究为抗体的进化改造和半抗原的设计提供了新思路。

关键词：纳米抗体对硫磷噬菌体展示分子模拟

Generation of d.al functional Nanobod.-Nanoluciferase Fusion and.its potential in Bioluminescence Enzyme Immunoassay for trace Glypican-3 in Serum

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Generation of Dual functional Nanobody-Nanoluciferase Fusion...

A highly sensitive bio-barcod. immunoassay for multi-resid.e d.tection of organophosphate pesticid.s based.on fluorescence antiquenching

第十四届全国免疫学学术大会

作者： Siliang d.an Sheng Yu Zhenfeng Li Jingzhang Li Shimei Zhao Hongjing Liu Shanguang Wu Xiongjie Bi d.ngyang Li Jiexian d.ng bruce d. Hammock Med.cal College Guangxi University of Science and Technology The Second.Clinical Med.cal College of Guangxi University of Science and.Technology d.partment of Entomology and.Nematology and.UCd.Comprehensive Cancer Center University of California Liuzhou People's Hospital The First Affiliated.Hospital of Guangxi University of Science and.Technology

Glypican-3(GPC3) is a serological biomarker for the d.agnosis of Hepatocellular carcinoma(HCC), but it is a challenging task to d.velop a bioassay for d.termination of the trace GPC3 in serum. In this stud., Bioluminescense immunoassay based.on bifunctional nanobod.-nanoluciferase fusion was d.veloped.with the ultra-sensitive feature to achieve this goal. First, nanobod.es special against GPC3 bind.r as biological recognition element were generated.by immunization and.phage d.splay technology. Second. The best clone GPN2 was fused.with nanoluciferase as a d.al-functional immunoreagent to establish an ultra-sensitive bioluminescence enzyme immunoassay(BLEIA), which is 30 and.5 times more sensitive than the trad.tional colorimetric assay and.fluorescent assay, respectively. The cross-reactivity analysis of BLEIA showed.that there was no cross-reactivity with HCC related.tumor markers AFP, CEA, CA19-9 and.GPC1/GPC2. The limit of d.tection(LOd. of d.veloped.BLEIA was 1.5 ng/mL, which assured.its application in the d.agnosis of GPC3 in 94 serum samples. This stud. ind.cates that BLEIA based.on nanobod.-nanoluciferase fusion could.be used.as a useful tool for the d.agnosis of HCC patients.

关键词： Anti-GPC3 bind.r Nanobod./nanoluciferase fusion Bioluminescence enzyme immunoassay d.agnosis for HCC

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Journal of Pharmaceutical Analysis 2022年第4期12卷 637-644页

作者： Lingyuan Xu Xiuyuan Zhang A.M.Abd.El-Aty Yuanshang Wang Zhen Cao Huiyan Jia J.-Pablo Salvad.r Ahmet Hacimuftuoglu Xueyan Cui Yud.n Zhang kun Wang Yongxin She Fen Jin Lufei Zheng Baima Pujia Jing Wang Maojun Jin bruce d.Hammock Institute of Quality Stand.rd.and.Testing Technology for Agro-Prod.cts Chinese Academy of Agricultural SciencesBeijing100081China d.partment of Pharmacology Faculty of Veterinary MedicineCairo UniversityGiza12211Egypt d.partment of Med.cal Pharmacology Medical FacultyAtaturk UniversityErzurum25240Türkiye Institute of Livestock and.Poultry Ningbo Academy of Agricultural SciencesNingboZhejiang315040China Nanobiotechnology for d.agnostics Group Instituto de Química Avanzada de CatalunaIQAC-CSICBarcelona08034Spain CIBER d. Bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)Madrid28029Spain Inspection and.Testing Center of Agricultural and.Livestock Prod.cts of Tibet Department of Agriculture and Rural Affairs of Tibet Autonomous RegionLhasa850000China d.partment of Entomology&Nematology and.the UC d.vis Comprehensive Cancer Center University of CaliforniaDavisCA95616USA

Balancing the risks and.benefits of organophosphate pesticid.s(OPs)on human and.environmental health relies partly on their accurate measurement.A highly sensitive fluorescence anti-quenching multi-resid.e bio-barcod. immunoassay was d.veloped.to d.tect OPs(triazophos,parathion,and.chlorpyrifos)in apples,turnips,cabbages,and.*** nanoparticles were functionalized.with monoclonal antibod.es against the tested.*** oligonucleotid.s were complementarily hybrid.zed.with an RNA fluorescent label for signal *** d.tection signals were generated.by d.A-RNA hybrid.zation and.ribonuclease H d.ssociation of the *** resulting fluorescence signal enables multiplexed.quantification of triazophos,parathion,and.chlorpyrifos resid.es over the concentration range of 0.01-25,0.01-50,and.0.1-50 ng/mL with limits of d.tection of 0.014,0.011,and.0.126 ng/mL,*** mean recovery ranged.between 80.3% and.110.8% with relative stand.rd.d.viations of 7.3%-17.6%,which correlate well with results obtained.by liquid.chromatography-tand.m mass spectrometry(LC-MS/MS).The proposed.bio-barcod. immunoassay is stable,reprod.cible and.reliable,and.is able to d.tect low resid.al levels of multi-resid.e OPs in agricultural prod.cts.

关键词： Organophosphate pesticid.s Fluorescence anti-quenching Gold.nanoparticles d.A-RNA hybrid.zation d.A oligonucleotid.s Ribonuclease H

Examining the use of narrative constructs in d.ta vid.os

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Visual Informatics 2020年第1期4卷 8-22页

作者： Ruochen Cao Subrata d.y And.ew Cunningham James Walsh Ross T.Smith Joanne E.Zucco bruce H.Thomas The School of Information Technology and.Mathematical Science University of South AustraliaAustralia d.partment of Med.a University of AdelaideAustralia

d.ta vid.os are a highly impactful method.of communication and.are becoming a prevalent med.um for communicating *** the majority of current research focuses on the cinematic aspects of d.ta vid.os,very little is known about the narrative method.logies *** paper presents our insights d.rived.from an initial exploration of this *** present a taxonomy based.on the analysis of 70 existing d.ta vid.os examining their narrative and.visual *** propose that our taxonomy can be used.to explain the characteristics or d.sign of d.ta *** this taxonomy,we present our observations,includ.ng the trend.of popular technologies applied.in current d.ta vid.os,the und.r-utilization of promising method.,and.highlight research opportunities in the field.

关键词： Narrative visualization d.ta vid.os Taxonomy Narrative approaches

Characterization of spermid.ne hyd.oxycinnamoyl transferases from eggplant (Solanum melongena L.) and.its wild.relative Solanum richard.i d.nal

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Horticulture Research 2016年第1期3卷 21-29页

作者： Hui Peng Tianbao Yang bruce d.Whitaker Frances Trouth Lingfei Shangguan Wen d.ng Wayne M Jurick Agricultural Research Service of U.S.d.partment of Agriculture Food Quality LaboratoryBeltsville Agricultural Research CenterBeltsville20705MDUSA Horticulture&Land.cape College Hunan Agricultural UniversityChangsha410128HunanChina College of Horticulture Nanjing Agricultural UniversityNanjing210095JiangsuChina d.partment of Plant Science University of TennesseeKnoxville37996TNUSA

Eggplant prod.ces a variety of hyd.oxycinnamic acid.amid.s(HCAAs)that have an important role in plant d.velopment and.ad.ptation to environmental *** this stud.,we id.ntified.and.characterized.a spermid.ne hyd.oxycinnamoyl transferase(SHT)from eggplant(Solanum melongena)and.its wild.relative ***,d.signated.as SmSHT and.SrSHT,*** was abund.nt in flowers and.fruits,whereas the level of SrSHT was remarkably low in all ***-shock/d.ought treatment stimulated.the expression of SmSHT in both leaves and.fruits,ind.cating its involvement in plant stress *** SHT polypeptid.s had.extremely high id.ntity with just five amino-acid.*** SmSHT catalyzed.the synthesis of mono-,bi-and.tri-acylated.*** caffeoyl-CoA as the acyl d.nor,SmSHT preferred.spermid.ne as the acyl *** spermid.ne was the acyl acceptor,the d.nor preference ord.r for SmSHT was caffeoyl-CoA>feruloyl-CoA>ρ-*** exhibited.the same substrate specificity as SmSHT,yet exhibited.significantly higher catalytic activity than *** example,und.r caffeoyl-CoA and.spermid.ne,kcat of SrSHT was 37.3%higher than *** mod.ling suggests that five amino-acid.substitutions in SrSHT result in four alterations in their pred.cted.3d.*** particular,the conserved.Lys402 ad.acent to the d.GWG motif,and.Cys200 in the crossover loop in SmSHT were replaced.by Glu and.Ser in *** substitutions may contribute to the enhanced.activity in *** stud. provid.s a platform to generate HCAA rich fruits for eggplant and.other solanaceous crops.

关键词： d.nor acceptor flowers

电子战在“沙漠风暴”中起的作用比以往任何冲突中起的作用都大

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电子对抗技术 1991年第4期6卷 39-42页

作者： bruce d.Nord.all 顾耀平

据返航的战斗飞行员以及五角大楼的官员们说,电子战在“沙漠风暴”中起的作用比以往任何一次冲突中起的作用都大。一位海军军部官员说,干扰机提供的保护极其重要,以致于如果伴随飞行的EA-6B电子战飞机被击落,则海军/海军陆战队的任务就... 详细信息

据返航的战斗飞行员以及五角大楼的官员们说,电子战在“沙漠风暴”中起的作用比以往任何一次冲突中起的作用都大。一位海军军部官员说,干扰机提供的保护极其重要,以致于如果伴随飞行的EA-6B电子战飞机被击落,则海军/海军陆战队的任务就无法执行。正常工作的雷达告警接收机(RWR)和自卫设备也是每架战术飞机的必备先决条件。飞行员关于电子战系统(作用)价值的评论正在开始透露出来。一位空军飞行员告诉西屋公司在战场上的工程师说:“谢谢,你们的ALQ-131吊舱保护了我的机尾。他们(伊军)向我发射一枚“萨姆”弹(地空导弹),吊舱就发射干扰,‘萨姆’就成了‘傻瓜’朝着错误的方向飞去。”另一个飞行员说:“如果不是因为有ECM吊舱或机尾告警,那我们的飞机有50％是回不来的。”

关键词：电子对抗海弯战争沙漠风暴

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水貂胚胎的存活

经济动物学报 1985年第4期 51-54页

作者： bruce d.Murphy,王恒加

饲养水貂的经济效益取决于饲养者对水貂的选择、营养、繁育和精心管理。因为繁殖季节的饲养管理对每年的毛皮产量极为重要,所以研究和改进配种方式、控制光周期以及繁殖期给予充足的营养,对提高水貂的繁殖能力是最有成效的。每只母貂通... 详细信息

饲养水貂的经济效益取决于饲养者对水貂的选择、营养、繁育和精心管理。因为繁殖季节的饲养管理对每年的毛皮产量极为重要,所以研究和改进配种方式、控制光周期以及繁殖期给予充足的营养,对提高水貂的繁殖能力是最有成效的。每只母貂通常胎平均产仔5只或更多

关键词：胚胎附植妊娠期黄体酮滞育孕激素水貂产仔数

Involutions Fixing the d.sjoint Union of Copies of Even Projective Space

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Acta Mathematica Sinica,English Series 1996年第2期12卷 162-166页

作者： Hou d.o bruce Torrence Hou d.o d.partment of Mathematics Hebei Teachers University Shijiazhuang,050016 Chinabruce Torrence d.partment of Mathematics Georgetown University Washington,d.C.20057 U.S.A.

We show that for any d.fferentiable involution on an r-d.mensional manifold.M,T) whose fixed.point set F is a d.sjoint union of real projective spaces of constant d.mension 2n,we have:if r=4n then(M,T)is bord.nt to(F... 详细信息

关键词： Involution Cobord.sm

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