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Waterlogging tolerance and wood properties of transgenic Populus alba×glandulosa expressing Vitreoscilla hemoglobin gene(Vgb)

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Journal of Forestry Research 2021年第2期32卷 831-839页

作者： Yiliang Li Weixi zhang Wenxu Zhu bingyu zhang Qinjun Huang Xiaohua Su State Key Laboratory of Tree Genetics and Breeding Research Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China Key Laboratory of Tree Breeding and Cultivation National Forestry and Grassland AdministrationBeijing 100091People’s Republic of China Guangdong Provincial Key Laboratory of Silviculture Protection and UtilizationGuangzhou 510520People’s Republic of China Guangdong Academy of Forestry Guangzhou 510520People’s Republic of China College of Forestry Shenyang Agricultural University120 Dongling StreetShengyang 110866People’s Republic of China

Because overexpression of Vitreoscilla hemoglobin gene(Vgb)gene in plants can enhance tolerance to waterlogging,here Vgb was inserted into Populus alba×glandulosa to investigate its expression and effects on growth and physiological responses to waterlogging stress in the transgenic *** blotting and RT-PCR analysis of Vgb-transgenic ***×glandulosa showed that the Vgb gene was integrated into the genome of the V13-81 and V13-85 transgenic lines and *** greenhouse waterlogging stress tests,mortality of the transgenic poplar was significant lower than that of nontransgenic plants with increasing treatment time from 2 to 22 *** transgenic plants had higher chlorophyll content and less chloroplast damage than in the control ***,starch accumulation increased,and growth was enhanced in the transgenic plants,suggesting that the Vgb-expressing lines had improved energy *** trials of the transgenic poplar suggested that Vgb expression promotes growth and influences wood *** together,our results suggest that the expression of Vgb can increase the accumulation of chlorophyll and starch in the transgenic poplar,improve its ability to endure flooding,and improve growth and wood quality of the transgenic plants.

关键词： Populus alba×glandulosa vgb gene Waterlogging stress Growth Microscopy

NONHOMOGENEOUS BOUNDARY VALUE PROBLEMS FOR THE KORTEWEG-DE VRIES EQUATION ON A BOUNDED DOMAIN

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Journal of Systems Science & Complexity 2010年第3期23卷 499-526页

作者： Eugene F. KRAMER bingyu zhang Department of Mathematics Physics and Computer Science University of Cincinnati Raymond Walters College Cincinnati Ohio 45236 USA Department of Mathematical Sciences University of Cincinnati Cincinnati Ohio 45221 USA

This paper studies an initial-boundary-value problem (IBVP) of the Korteweg-de Vriesequation posed on a finite interval with general nonhomogeneous boundary *** thestrong Kato smoothing property of the associated linear problem,the IBVP is shown to be locallywell-posed in the space H^s(0,1) for any s≥0 via the contraction mapping principle.

关键词： KdV equation Korteweg-de Vries equation well-posed.

A cDNA microarray analysis of the molecular control of poplar wood properties

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Journal of Forestry Research 2017年第1期28卷 71-82页

作者： Qinjun Huang Changjun Ding Weixi zhang bingyu zhang Yanguang Chu Dahai Wang Xiaohua Su State Key Laboratory of Tree Genetics and Breeding Research Institute of ForestryChinese Academy of ForestryBeijing 100091China Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Beijing 100091China

Molecular biological research into wood development and formation has been the focus in recent years, but the pace of discovery of related genes and their functions in the control of wood properties has been slow. The microarray technique--with its advantages of high throughput capacity, sensitivity, and reliability over other tools developed for investigating genes expression patterns-is capable of rapidly assaying thousands of genes. In this study, a cDNA microarray prepared from two cDNA libraries of developing poplar xylem tissues was used to assay gene expression patterns in immature xylem tissues at different heights from the main stem of Populus deltoides （15 years old）, which was confirmed to have distinct wood properties （microfibrillar angle, woody density） by X-ray. Two hundred seventy-four transcripts with differ- ential expression profles between the chips were screened out, and the individual clones were subjected to 5＇ sequencing. Using bioinformatic analysis, we identified candidate genes that may influence poplar wood properties, many of which belong to various regulatory and signal transduction gene families, such as zinc finger protein transcription factor, DNA-binding transcription factor, ethylene response factors, and so on. The results suggest that these genes may regulate enzymes involved in wood formation. Further work will be performed to clone these genes and determine how they influence poplar wood properties.

关键词： Populus deltoides cDNA microarray Expression profile Wood density Microfibfillar angle

Assessing bacterial communities in the rhizosphere of 8-year-old genetically modified poplar(Populus spp.)

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Journal of Forestry Research 2016年第4期27卷 939-947页

作者： Wenxu Zhu Yanguang Chu Changjun Ding Qinjun Huang bingyu zhang Weixi zhang Xiaohua Su State Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry Chinese Academy of ForestryBeijing 100091 China Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Beijing 100091 China

Microbe communities in rhizosphere ecosystems are important for plant health but there is limited knowledge of them in the rhizospheres of genetically modified（GM） plants, especial for tree species. We used the amplitude sequencing method to analyze the V4 regions of the 16 S r RNA gene to identify changes in bacterial diversity and community structure in two GM lines（D520 and D521）, one non-genetically modified（nonGM） line and in uncultivated soil. After chimera filtering,468.133 sequences in the domain Bacteria remained. There were ten dominant taxonomic groups（with [1 % of all sequences） across the samples. 241 of 551 genera（representing a ratio of 97.33 %） were common to all samples.A Venn diagram showed that 1.926 operational taxonomic units（OTUs） were shared by all samples. We found a specific change, a reduction in Chloroflexi, in the microorganisms in the rhizosphere soil planted with poplars. Taken together, the results showed few statistical differences in the bacterial diversity and community structure between the GM line and non-GM line, this suggests that there was no or very limited impact of this genetic modification on the bacterial communities in the rhizosphere.

关键词： rhizosphere communities Bacteria sequencing poplar planted taxonomic representing genera microbial

Fluorescence Detection for H_2PO_4^- based on Carbon Dots/Fe^(3＋) Composite

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Journal of Wuhan University of Technology(Materials Science) 2016年第6期31卷 1226-1229页

作者：范小春 zhang bingyu 丁莉芸 XU Chuang HUANG Jun School of Civil Engineering and Architecture Wuhan University of Technology Wuhan 430070 China National Engineering Laboratory for Fiber Optic Sensing Technology Wuhan University of Technology Wuhan 430070 China

A novel fluorescent probe for H_2PO_4^- was designed and fabricated based on the carbon dots/Fe^（3＋） composite. The carbon dots were synthesized by an established one-pot hydrothermal method and characterized by transmission electron microscope, X-ray diffractometer, UV-Vis absorption spectrometer and fluorescence spectrophotometer. The carbon dots/Fe^（3＋） composite was obtained by aqueous mixing of carbon dots and FeCl_3, and its fluorescence property was characterized by fluorescence spectrophotometer. The fluorescence of carbon dots was quenched by aqueous Fe^（3＋） cations, resulting in the low fluorescence intensity of the carbon dots/Fe^（3＋） composite. On the other hand, H_2PO_4^- reduced the concentration of Fe^（3＋） by chemical reaction and enhanced the fluorescence of the carbon dots/Fe^（3＋） composite. The Stern-Volmer equation was introduced to describe the relation between the relative fluorescence intensity of the carbon dots/Fe^（3＋） composite and the concentration of H_2PO_4^-, and a fine linearity（R2=0.997） was found in the range of H_2PO_4^- concentration of 0.4-12 m M.

关键词： H2PO4-detection fluorescence carbon dots/Fe3+ composite

Application of UAV Multisensor Data and Ensemble Approach for High-Throughput Estimation of Maize Phenotyping Traits

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Plant Phenomics 2022年第1期4卷 110-126页

作者： Meiyan Shu Shuaipeng Fei bingyu zhang Xiaohong Yang Yan Guo Baoguo Li Yuntao Ma College of Land Science and Technology China Agricultural UniversityBeijing 100091China State Key Laboratory of Plant Physiology and Biochemistry National Maize Improvement Center of ChinaChina Agricultural UniversityBeijing 100091China

High-throughput estimation of phenotypic traits from UAV(unmanned aerial vehicle)images is helpful to improve the screening efficiency of breeding *** estimating phenotyping traits of breeding maize at plot scale helps to promote gene mining for specific traits and provides a guarantee for accelerating the breeding of superior *** an efficient and accurate estimation model is the key to the application of UAV-based multiple sensors data.

关键词： breeding estimation traits

The Axis Design and Layout of Automotive Door Hinges

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The Axis Design and Layout of Automotive Door Hinges

Multiple transgenes Populus xeuramericana 'Guariento' plants obtained by biolistic bombardment

2014中国汽车工程学会年会

作者： zhang bingyu Yu Wei Wang Yuanhong Changan Auto R＆D Center

The method for automotive door hinge axis design and layout is *** example of a front door hinge assembly is presented to show the detailed strategy and influence factors in door hinge axis design and layout,and then ... 详细信息

ISBN: (纸本)9783662450420

关键词： door hinge axis design layout

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Chinese Science Bulletin 2007年第2期52卷 224-230页

作者： Wang JianGe Su XiaoHua Ji LiLi zhang bingyu Hu ZanMin Huang RongFeng Tian YingChuan Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 China College of Landscape and art Jiangxi Agricultural University Nanchang 330045 China Institute of Genetics and developmental biology Chinese Academy of Sciences Beijing 100101 China Biotechnology Research Institute Chinese Academy of Agricultural Science Beijing 100081 China Microbiology Institute Chinese Academy of Sciences Beijing 100080 China

A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana 'Guariento' using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P. xeuramericana 'Guariento' and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land.

关键词：森林生态系统全球变暖二氧化碳植被

No consistent daily variation in DNA methylation detected in Populus nigra leaves by methylation-sensitive amplification polymorphism analysis

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Journal of Forestry Research 2017年第4期28卷 653-660页

作者： Shu Diao Yanbo Wang Changjun Ding Yingying Chang Lixiong Liang Yanan Gao bingyu zhang Xiaohua Su State Key Laboratory of Tree Genetics and Breeding Research Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China

DNA methylation, an epigenetic mechanism used by cells to control gene expression, has an important biological role in plant development and environmental fitness. Since plant DNA methylation is closely related to environmental conditions, variation during the day is expected. Here, in genetically identical plants of Populus nigra clone N46, DNA methylation changes in leaves over a 24 h period were detected using the methylation-sensitive amplification polymorphism method. The results showed different DNA methylation patterns in mature poplar leaves: not only in individuals at the same time, but also in samples at each of the six time during the day. In addition, night samples had a higher percentage of methylation than in morning samples. However, no statistically significant differences were found among the samples gathered at different times. Similar results were obtained for three other P. nigra clones with different genetic backgrounds. Real time qPCR showed that the DNA methyltransferase genes Pt-MET1 and Pt-SOM1 involved in CG DNA methylation in poplar were stable over a 24 h period in leaves of P. nigra N46 compared with circadian-controlled genes. That could be part of the reason that methylation of CCGG sites is stable in those leaves. That DNA methylation differed even in genetically identical plants indicates the specificity of DNA methylation changes in their genomes. No statistically significant differences in methylation changes were found between day and night, suggesting that DNA methylation is more stable than expected and is unlikely to be involved in circadian regulation in plants.

关键词： Cytosine methylation Epigenetic Populus nigra Methylation-sensitive amplification polymorphism (MSAP) Variation