Multiple transgenes Populus xeuramericana 'Guariento' plants obtained by biolistic bombardment

作     者：Wang JianGe Su XiaoHua Ji LiLi Zhang BingYu Hu ZanMin Huang RongFeng Tian YingChuan 

作者机构：Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 China College of Landscape and art Jiangxi Agricultural University Nanchang 330045 China Institute of Genetics and developmental biology Chinese Academy of Sciences Beijing 100101 China Biotechnology Research Institute Chinese Academy of Agricultural Science Beijing 100081 China Microbiology Institute Chinese Academy of Sciences Beijing 100080 China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2007年第52卷第2期

页      面：224-230页

核心收录：

学科分类：07[理学] 070602[理学-大气物理学与大气环境] 0706[理学-大气科学] 

主　　题：森林 生态系统 全球变暖 二氧化碳 植被 

摘      要：A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana Guariento using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P. xeuramericana Guariento and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land.