Waterlogging tolerance and wood properties of transgenic Populus alba×glandulosa expressing Vitreoscilla hemoglobin gene(Vgb)

作     者：Yiliang Li Weixi Zhang Wenxu Zhu Bingyu Zhang Qinjun Huang Xiaohua Su Yiliang Li;Weixi Zhang;Wenxu Zhu;Bingyu Zhang;Qinjun Huang;Xiaohua Su

作者机构：State Key Laboratory of Tree Genetics and BreedingResearch Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China Key Laboratory of Tree Breeding and CultivationNational Forestry and Grassland AdministrationBeijing 100091People’s Republic of China Guangdong Provincial Key Laboratory of SilvicultureProtection and UtilizationGuangzhou 510520People’s Republic of China Guangdong Academy of ForestryGuangzhou 510520People’s Republic of China College of ForestryShenyang Agricultural University120 Dongling StreetShengyang 110866People’s Republic of China 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2021年第32卷第2期

页      面：831-839页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：supported by the Basic Research Fund of RIF(Grant No.CAFYBB2017ZA001-3) the National Natural Science Foundation of China(Grant No.31700589) the Forestry Genetic Breeding National Key Laboratory(Chinese Academy of Forestry Sciences)Open Project(Grant No.TGB 2013005) 

主　　题：Populus alba×glandulosa vgb gene Waterlogging stress Growth Microscopy 

摘      要：Because overexpression of Vitreoscilla hemoglobin gene(Vgb)gene in plants can enhance tolerance to waterlogging,here Vgb was inserted into Populus alba×glandulosa to investigate its expression and effects on growth and physiological responses to waterlogging stress in the transgenic *** blotting and RT-PCR analysis of Vgb-transgenic ***×glandulosa showed that the Vgb gene was integrated into the genome of the V13-81 and V13-85 transgenic lines and *** greenhouse waterlogging stress tests,mortality of the transgenic poplar was significant lower than that of nontransgenic plants with increasing treatment time from 2 to 22 *** transgenic plants had higher chlorophyll content and less chloroplast damage than in the control ***,starch accumulation increased,and growth was enhanced in the transgenic plants,suggesting that the Vgb-expressing lines had improved energy *** trials of the transgenic poplar suggested that Vgb expression promotes growth and influences wood *** together,our results suggest that the expression of Vgb can increase the accumulation of chlorophyll and starch in the transgenic poplar,improve its ability to endure flooding,and improve growth and wood quality of the transgenic plants.