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Comparative Transcriptomic Profiling of Vitis vinifera Under High Light Using a Custom-Made Array and the Affymetrix GeneChip

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Molecular Plant 2011年第6期4卷 1038-1051页

作者： Luisa C. Carvalho belmiro j. vilela Phil M. Mullineaux Sara Amancio CBAA Instituto Superior de Agronomia Universidade Tecnica de Lisboa Tapada da Ajuda 1349-017 Lisboa Portugal DDSB John Innes Centre Colney Norwich NR4 7UH UK Department of Biological Sciences University of Essex Colchester CO4 3SQ UK

Understanding abiotic stress responses is one of the most important issues in plant research nowadays. Abiotic stress, including excess light, can promote the onset of oxidative stress through the accumulation of reactive oxygen species. Oxidative stress also arises when in vitro propagated plants are exposed to high light upon transfer to ex vitro. To determine whether the underlying pathways activated at the transfer of in vitro grapevine to ex vitro conditions reflect the processes occurring upon light stress, we used Vitis vinifera Affymetrix GeneChip （VvGA） and a custom array of genes responsive to light stress （LSCA） detected by real-time reverse transcriptase PCR （qRT-PCR）. When gene-expression profiles were compared, ＇protein metabolism and modification＇, ＇signaling＇, and ＇anti-oxidative＂ genes were more represented in LSCA, while, in VvGA, ＇cell wall metabolism＇ and ＇secondary metabolism＇ were the categories in which gene expression varied more significantly. The above functional categories confirm previous studies involving other types of abiotic stresses, enhancing the common attributes of abiotic stress defense pathways. The LSCA analysis of our experimental system detected strong response of heat shock genes, particularly the protein rescuing mechanism involving the cooperation of two ATP-dependent chaperone systems, Hsp100 and Hsp70, which showed an unusually late response during the recovery period, of extreme relevance to remove non-functional, potentially harmful polypeptides arising from misfolding, denaturation, or aggregation brought about by stress. The success of LSCA also proves the feasibility of a custommade qRT-PCR approach, particularly for species for which no GeneChip is available and for researchers dealing with a specific and focused problem.

关键词： Quantitative real-time PCR Vitis vinifera GeneChip light stress Heat Shock Proteins oxidative and photooxidative stress.

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Casein Kinase 2 Negatively Regulates Abscisic Acid-Activated SnRK2s in the Core Abscisic Acid-Signaling Module

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Molecular Plant 2015年第5期8卷 709-721页

作者： belmiro vilela Elena Naj.r Victoria Lumbreras j.ffrey Leung Montserrat Pages Centre for Research in Agricultural Genomics Parc de Recerca UAB Edifici CRAG Campus UAB Bellaterra (Cerdanyola del Vallea) 08193 Barcelona Spain ISV - Institut de Sciences du Vegetal CNRS bat 23 avenue de la Terrasse 91198 Gif-sur-Yvette Cedex France

SnRK2 kinases, PP2C phosphatases and the PYR/PYL/RCAR receptors constitute the core abscisic acid （ABA） signaling module that is thought to contain all of the intrinsic properties to self-regulate the hormone signal output. Here we identify Casein Kinase （CK）2 as a novel negative regulator of SnRK2. CK2 phosphor- ylates a cluster of conserved serines at the ABA box of SnRK2, increasing its binding to PP2C and triggering protein degradation. Consequently, CK2 action has implications on SnRK2 protein levels, as well as kinase activity and its response to abiotic stimuli.

关键词： abiotic stress maize protein phosphorylation

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