Comparative Transcriptomic Profiling of Vitis vinifera Under High Light Using a Custom-Made Array and the Affymetrix GeneChip

作     者：Luisa C. Carvalho Belmiro J. Vilela Phil M. Mullineaux Sara Amancio 

作者机构：CBAA Instituto Superior de Agronomia Universidade Tecnica de Lisboa Tapada da Ajuda 1349-017 Lisboa Portugal DDSB John Innes Centre Colney Norwich NR4 7UH UK Department of Biological Sciences University of Essex Colchester CO4 3SQ UK 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2011年第4卷第6期

页      面：1038-1051页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Fundação para a Ciência e Tecnologia European Regional Development Fund, FEDER, (SFRH/BPD/5707/2001, PTDC/AGR-GPL/099624/2008, POCTI/AGG/37968/2001) , (SFRH/BPD/5707/2001, POCTI/AGG/37968/2001) 

主　　题：Quantitative real-time PCR Vitis vinifera GeneChip light stress Heat Shock Proteins oxidative and photooxidative stress. 

摘      要：Understanding abiotic stress responses is one of the most important issues in plant research nowadays. Abiotic stress, including excess light, can promote the onset of oxidative stress through the accumulation of reactive oxygen species. Oxidative stress also arises when in vitro propagated plants are exposed to high light upon transfer to ex vitro. To determine whether the underlying pathways activated at the transfer of in vitro grapevine to ex vitro conditions reflect the processes occurring upon light stress, we used Vitis vinifera Affymetrix GeneChip （VvGA） and a custom array of genes responsive to light stress （LSCA） detected by real-time reverse transcriptase PCR （qRT-PCR）. When gene-expression profiles were compared, ＇protein metabolism and modification＇, ＇signaling＇, and ＇anti-oxidative＂ genes were more represented in LSCA, while, in VvGA, ＇cell wall metabolism＇ and ＇secondary metabolism＇ were the categories in which gene expression varied more significantly. The above functional categories confirm previous studies involving other types of abiotic stresses, enhancing the common attributes of abiotic stress defense pathways. The LSCA analysis of our experimental system detected strong response of heat shock genes, particularly the protein rescuing mechanism involving the cooperation of two ATP-dependent chaperone systems, Hsp100 and Hsp70, which showed an unusually late response during the recovery period, of extreme relevance to remove non-functional, potentially harmful polypeptides arising from misfolding, denaturation, or aggregation brought about by stress. The success of LSCA also proves the feasibility of a custommade qRT-PCR approach, particularly for species for which no GeneChip is available and for researchers dealing with a specific and focused problem.