检索结果-南通市图书馆

Comparative Transcriptomic Profiling of Vitis vinifera Under High Light Using a Custom-Made Array and the Affymetrix GeneChip

引用

Molecular Plant 2011年第6期4卷 1038-1051页

作者： Luisa C. Carvalho Belmiro J. Vilela Phil M. Mullineaux Sara Amancio CBAA Instituto Superior de Agronomia Universidade Tecnica de Lisboa Tapada da Ajuda 1349-017 Lisboa Portugal ddsb John Innes Centre Colney Norwich NR4 7UH UK Department of Biological Sciences University of Essex Colchester CO4 3SQ UK

Understanding abiotic stress responses is one of the most important issues in plant research nowadays. Abiotic stress, including excess light, can promote the onset of oxidative stress through the accumulation of reactive oxygen species. Oxidative stress also arises when in vitro propagated plants are exposed to high light upon transfer to ex vitro. To determine whether the underlying pathways activated at the transfer of in vitro grapevine to ex vitro conditions reflect the processes occurring upon light stress, we used Vitis vinifera Affymetrix GeneChip （VvGA） and a custom array of genes responsive to light stress （LSCA） detected by real-time reverse transcriptase PCR （qRT-PCR）. When gene-expression profiles were compared, ＇protein metabolism and modification＇, ＇signaling＇, and ＇anti-oxidative＂ genes were more represented in LSCA, while, in VvGA, ＇cell wall metabolism＇ and ＇secondary metabolism＇ were the categories in which gene expression varied more significantly. The above functional categories confirm previous studies involving other types of abiotic stresses, enhancing the common attributes of abiotic stress defense pathways. The LSCA analysis of our experimental system detected strong response of heat shock genes, particularly the protein rescuing mechanism involving the cooperation of two ATP-dependent chaperone systems, Hsp100 and Hsp70, which showed an unusually late response during the recovery period, of extreme relevance to remove non-functional, potentially harmful polypeptides arising from misfolding, denaturation, or aggregation brought about by stress. The success of LSCA also proves the feasibility of a custommade qRT-PCR approach, particularly for species for which no GeneChip is available and for researchers dealing with a specific and focused problem.

关键词： Quantitative real-time PCR Vitis vinifera GeneChip light stress Heat Shock Proteins oxidative and photooxidative stress.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：