检索结果-南通市图书馆

Chinese Journal of Clinical Oncology 2007年第3期4卷 210-215页

作者： Hui sun Jing Zhang Zhongli Zhan baocun sun Xishan Hao Pathology Department Tianjin Medica University Cancer Hospital Tianjin 300060 China

OBJECTIVE To investigate the relationship between the level of cas-pase-3 and 8 expression and intracellular Ca2+ levels in BCML-TA299 breast cancer cells in the process of apoptosis. METHODS Mice were divided into three IFNа-treated groups and one control group as follows: an intratumoral injection, subcutaneous injection, preventive injection, and a control without injection. The cellular DNA content, changes in the cell cycle and the relationship between the cell Ca2+ concen-trations and the expression of caspase-3 and 8 were examined. RESULTS After injection of IFN-α-2b by different routes, the morphologic transformation of the breast cancer cells in each group was observed. There was a typical apoptotic response in the intratumoral-injection group. The expression of caspase-3 and 8 was diverse among the experimental groups, and correlated with the cellular Ca2+ concentration. Caspase-3 and 8 expres-sion and the cellular Ca2+ level were higher following intratumoral injection compared to the other treatments (P<0.01). Among the experimental groups, the cell cycle displayed definitive changes. CONCLUSION a) Both caspase-3 and 8 and the intracellular Ca2+ are elevated in the process of cell apoptosis in BCML-TA299 breast cancer tis-sues. These changes may play important roles in the occurrence and devel-opment of breast cancer; b) Variation in the route of IFN-α-2b administration can produce different responses in the expression of caspase-3 and 8 and the concentration of Ca2+ in apoptotic tumor cells.

关键词：乳腺癌钙离子细胞凋亡基因表达

Hypoxic microenvironment induced spatial transcriptome changes in pancreatic cancer

在线全文

学校读者我要写书评

暂无评论

Cancer Biology & Medicine 2021年第2期18卷 616-630页

作者： Huizhi sun Danfang Zhang Chongbiao Huang Yuhong Guo Zhao Yang Nan Yao Xueyi Dong Runfen Cheng Nan Zhao Jie Meng baocun sun Jihui Hao Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin’s Clinical Research Center for CancerTianjin 300060China Department of Pathology School of Basic Medical ScienceTianjin Medical UniversityTianjin 300070China

Objective: Hypoxia is a significant feature of solid tumors, including pancreatic ductal adenocarcinoma(PDAC). It is associated with tumor invasion, metastasis, and drug resistance. However, the spatial distribution of hypoxia-related heterogeneity in PDAC remains ***: Spatial transcriptomics(STs), a new technique, was used to investigate the ST features of engrafted human PDAC in the ischemic hind limbs of nude mice. Transcriptomes from ST spots in the hypoxic tumor and the control were clustered using differentially-expressed genes. These data were compared to determine the spatial organization of hypoxia-induced heterogeneity in PDAC. Clinical relevance was validated using the Tumor Cancer Genome Atlas and KM-plotter databases. The CMAP website was used to identify molecules that may serve as therapeutic targets for ***: ST showed that the tumor cell subgroups decreased to 7 subgroups in the hypoxia group, compared to 9 subgroups in the control group. Different subgroups showed positional characteristics and different gene signatures. Subgroup 6 located at the invasive front showed a higher proliferative ability under hypoxia. Subgroup 6 had active functions including cell proliferation, invasion, and response to stress. Expressions of hypoxia-related genes, LDHA, TPI1, and ENO1, induced changes. CMAP analysis indicated that ADZ-6482, a PI3 K inhibitor, was targeted by the invasive subgroup in hypoxic ***: This study is the first to describe hypoxic microenvironment-induced spatial transcriptome changes in PDAC, and to identify potential treatment targets for PDAC. These data will provide the basis for further investigations of the prognoses and treatments of hypoxic tumors.

关键词： Pancreatic cancer hypoxia spatial transcriptomic

人工智能在肿瘤组织病理标志物分析中的应用进展

在线全文

学校读者我要写书评

暂无评论

中国肿瘤临床 2022年第14期49卷 743-747页

作者：张艳辉吴江华孙保存天津医科大学肿瘤医院病理科国家恶性肿瘤临床医学研究中心天津市“肿瘤防治”重点实验室天津市恶性肿瘤临床医学研究中心天津市300060 天津医科大学病理教研室

肿瘤个体化治疗的进展对肿瘤组织病理标记物的精确诊断提出了更高的要求。数字病理(digital pathology,DP)的发展为人工智能(artificial intelligence,AI)辅助诊断在肿瘤组织病理图像分析中的应用提供了基础。基于卷积神经网络的深度学... 详细信息

肿瘤个体化治疗的进展对肿瘤组织病理标记物的精确诊断提出了更高的要求。数字病理(digital pathology,DP)的发展为人工智能(artificial intelligence,AI)辅助诊断在肿瘤组织病理图像分析中的应用提供了基础。基于卷积神经网络的深度学习(deep learning,DL)算法能够将DP图像与计算机分析技术相结合,有望成为定量评价肿瘤组织生物标志物的重要工具。本文概述了AI在组织病理学中的发展,并以目前研究相对广泛且与临床诊疗密切相关的分子病理指标Her-2、Ki-67及PD-L1的图像分析为具体案例,重点阐述了当前AI在肿瘤病理标志物分析中的研究进展。AI辅助的肿瘤病理诊断具有客观性强及可重复性高等优点,能够实现肿瘤组织标志物诊断的定量分析,从而克服病理医生人工判读的挑战,提高病理诊断的精确性。通过计算机工具构建肿瘤组织标志物的AI判读模式,是构建未来肿瘤智能诊疗体系的重要环节。

关键词：人工智能数字病理肿瘤标志物

A nomogram-based immune-serum scoring system predicts overall survival in patients with lung adenocarcinoma

在线全文

学校读者我要写书评

暂无评论

Cancer Biology & Medicine 2021年第2期18卷 517-529页

作者： Qiujuan Huang Tongyuan Qu Lisha Qi Changxu Liu Yuhong Guo Qianru Guo Guangning Li Yalei Wang Wenshuai Zhang Wei Zhao Danyang Ren Leina sun Shengguang Wang Bin Meng baocun sun Bin Zhang Wenjuan Ma Wenfeng Cao Department of Pathology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin’s Clinical Research Center for CancerTianjin 300060China Department of Pathology Tianjin Academy of Traditional Chinese Medicine Affiliated HospitalTianjin 300120China Department of Lung Cancer Tianjin Lung Cancer Center Department of Breast Cancer Department of Breast Imaging Key Laboratory of Breast Cancer Prevention and TherapyTianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin’s Clinical Research Center for CancerTianjin 300060China

Objective:The immunoscore,which is used to quantify immune infiltrates,has greater relative prognostic value than tumor,node,and metastasis(TNM)stage and might serve as a new system for classification of colorectal ***,a comparable immunoscore for predicting lung adenocarcinoma(LUAD)prognosis is currently ***:We analyzed the expression of 18 immune features by immunohistochemistry in 171 *** relationship of immune marker expression and clinicopathologic factors to the overall survival(OS)was analyzed with the Kaplan-Meier method.A nomogram was developed by using the optimal features selected by least absolute shrinkage and selection operator(LASSO)regression in the training cohort(n=111)and evaluated in the validation cohort(n=60).Results:The indicators integrated in the nomogram were TNM stage,neuron-specific enolase,carcino-embryonic antigen,CD8 center of tumor(CT),CD8 invasive margin(IM),Fox P3 CT,and CD45 *** calibration curve showed prominent agreement between the observed 2-and 5-year OS and that predicted by the *** simplify the nomogram,we developed a new immune-serum scoring system(I-SSS)based on the points awarded for each factor in the *** I-SSS was able to stratify same-stage patients into different risk *** combination of I-SSS and TNM stage had better prognostic value than the TNM stage ***:Our new I-SSS can accurately and individually predict LUAD prognosis and may be used to supplement prognostication based on the TNM stage.

关键词： Lung adenocarcinoma immune microenvironment nomogram immunoscore prognosis

在线全文

学校读者我要写书评

暂无评论

肺腺癌神经内分泌分化的临床意义(英文)

The Chinese-German Journal of Clinical Oncology 2012年第2期11卷 76-79页

作者： Yuanhuan Dong Leina sun baocun sun Dongxiang Yu Zhongli Zhan Department of Pathology Ninghe County Hospital Tianjin 301500 China Department of Pathology Tianjin Medical University Cancer Institute and Hospital Tianjin 300060 China

Objective:The aim of our study was to investigate the prevalence and clinical relevance of neuroendocrine(NE) differentiation in lung ***:Eighty-six adenocarcinoma paraffin-embedded specimens and cases which were followed up completely for 3 years,were obtained from 86 patients(35 men and 51 women) who underwent surgical resection for pathologically supported adenocarcinoma in the Cancer Hospital of Tianjin Medical University,from June 2005 to December *** EnVision two-step method was used to detect the expression of neuron-specific enolase(NSE),synaptophysin(SYN) and chromogranin A(CGA).All data were analyzed using SPSS statistics software and Kaplan-Meier survival curves were constructed,meanwhile,we conducted a Log-rank ***:All patients with lung adenocarcinoma,35 cases with NE differentiation(40.7%).The statistical analysis showed that the positive rate of NE differentiation in lung adenocarcinoma was significantly associated with cancer recurrence and histological *** addition,CGA,NSE and SYN positive rates were 27.9%,50.0%,43.0%,respectively.A statistically significant difference was found between positive expression of SYN and other clinicopathological parameters,such as pathological type,histological differentiation,lymph node metastasis,postoperative recurrence and 3-year survival rate(P = 0.001) and so ***:NE differentiation can be used as a metastatic potentially indicator of biological behavior of lung adenocarcinoma,and combined detection of NSE and SYN markers may be recommended to examine NE differentiation of lung *** expression of SYN indicates poor prognosis.

关键词：组织分化神经内分泌临床意义肺腺癌神经元特异性烯醇化酶 SPSS统计软件临床病理联合检测

在线全文

学校读者我要写书评

暂无评论

伴鳞样成分的甲状腺间变性癌临床病理分析

中国肿瘤临床 2011年第13期38卷 773-777页

作者：潘毅孙保存天津医科大学附属肿瘤医院病理科、天津市肿瘤防治重点实验室天津市300060

目的:探讨伴鳞样成分的甲状腺间变性癌(anaplastic thyroid carcinoma with squamoid eell component,ATC-SCC)的诊断、鉴别诊断及临床病理特征方法:回顾性分析85例少见甲状腺肿瘤的临床病理资料,通过HE切片进行形态学观察,对5例ATC-SC... 详细信息

目的:探讨伴鳞样成分的甲状腺间变性癌(anaplastic thyroid carcinoma with squamoid eell component,ATC-SCC)的诊断、鉴别诊断及临床病理特征方法:回顾性分析85例少见甲状腺肿瘤的临床病理资料,通过HE切片进行形态学观察,对5例ATC-SCC,6例甲状腺鳞状细胞癌(squamous cell carcinoma,SCC),8例甲状腺呈胸腺样分化癌(carcinoma showing thymus-like elements,CASTLE)和2例甲状腺降钙素阴性的神经内分泌肿瘤(calcitonin-negative neuroendocrine tumor of the thyroid gland,CNNETT)进行9项免疫组织化学染色,包括Cytokeratin(CK)、Vimentin(VM)、Chromogranin A(CgA)、Synaptophysin(SYN)、CD117、CD5、Calcitonin(CT)、Thyroglobulin(TG)和Thyroid transcription factor-l(TTF-1),并对结果进行分析结果:5例ATC-SCC中,男性2例,女性3例;年龄41～79岁,平均年龄53.2岁;肿物位于左腺叶3例,右腺叶2例。临床表现为无痛性颈部肿物。肿物通常体积较大,质地硬实,与周围组织粘连。镜下可见肿瘤组织由梭形细胞、多形性巨细胞、破骨样细胞和鳞状上皮样细胞以不同比例混合而成,并可见胞浆红染的横纹肌样细胞及大片坏死免疫组织化学染色显示5例ATC-SCC的肿瘤细胞CK和VM均阳性。鳞状上皮样细胞为主要成分时需要与SCC、CASTLE和CNNETT进行鉴别。结论:ATC-SCC多见于老年人,女性较多见,生长迅速,常扩展至甲状腺外累犯颈部软组织,预后非常差。

关键词： ATC-SCC 临床病理特征诊断鉴别诊断

Multidisciplinary team for the diagnosis and treatment of 2cases of primary intestinal yolk sac tumor

在线全文

学校读者我要写书评

暂无评论

Cancer Biology & Medicine 2018年第4期15卷 461-467页

作者： Qiujuan Huang Changxu Liu Ruijun Tang Jie Li Wei Li Lei Zhu Yuhong Guo Lin Zhang Tongyuan Qu Lingyi Yang Lingmei Li Yalei Wang baocun sun Bin Meng Jie Yan Wengui Xu Huilai Zhang Dengfeng Cao Wenfeng Cao Department of Pathology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerTianjin Medical UniversityMinistry of EducationTianjin 300060China Department of Pathology Guilin TCM Hospital of ChinaGuilin 541002China Department of Pediatric Oncology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerTianjin 300060China Department of Lymphoma Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerTianjin 300060China Department of Molecular Image and Nuclear Medicine Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerTianjin 300060China Department of Pathology Tianjin Central Hospital of Gynecology ObstetricsTianjin 300110China The Lauren V Ackerman Laboratory of Surgical Pathology Division of Anatomic and Molecular PathologyDepartment of Pathology and ImmunologyWashington University School of MedicineSaint LouisMO 63110USA

Extragonadal primary yolk sac tumor of the intestinal tract origin is exceedingly rare. Through a multiple disciplinary team, the diagnosis and treatment of primary intestinal yolk sac tumor were further defined. We report 2 such cases with detailed histologic and immunohistochemical analysis. The two patients were a 7-year-old girl and a 29-year-old woman. Both of them preoperatively had an elevated serum alpha fetoprotein(AFP) level(≥ 1,210 ng/mL). The tumors are located in the intestine and imaging examination indicated the rectum as the primary site. Grossly the mass was grey-white and crisp texture. Microscopic examination featured reticular, microcystic, macrocystic, papillary, solid, and some glandular patterns. Immunohistochemically,tumor cells of both cases were positive for SALL4, AFP, pan-cytokeratin(AE1/AE3), and glypican-3. Simultaneously, a stain for EMA, OCT4, CD30, HCG, vimentin and CK20 were negative in all 2 neoplasms. The features of morphology,immunohistochemistry, laboratory examinations and imaging studies consist of the diagnosis of primary yolk sac tumor of the intestine.

关键词： Yolk sac tumor intestine extragonadal immunohistochemistry multidisciplinary team (MDT)

在线全文

学校读者我要写书评

暂无评论

胃癌表观遗传学研究

中国肿瘤临床 2009年第11期36卷 656-658页

作者：张亮孙保存天津市第五中心医院病理科天津市300450 天津医科大学附属肿瘤医院

生物基因组中表观遗传学信息决定着表观基因型,为蛋白质的制造提供何时、何地、以何种方式行使遗传信息的指令。长期的分子生物学研究揭示了一系列表观遗传学的改变与胃癌的发生、发展有关,这些改变涉及抑癌基因、细胞粘附分子、细胞周... 详细信息

生物基因组中表观遗传学信息决定着表观基因型,为蛋白质的制造提供何时、何地、以何种方式行使遗传信息的指令。长期的分子生物学研究揭示了一系列表观遗传学的改变与胃癌的发生、发展有关,这些改变涉及抑癌基因、细胞粘附分子、细胞周期调节因子和遗传不稳定性等,相关表观遗传学分子机制主要包括DNA甲基化、基因组印记异常表达、组蛋白异常乙酰化、microRNA改变等。利用MSP等方法检测胃癌患者血清中相关基因异常甲基化是一种具有较高灵敏度和特异性的方法,可以作为胃癌诊断的辅助手段。DNA甲基化抑制剂和组蛋白去乙酰化酶抑制剂联合使用,能够更有效地抑制肿瘤的生长,有可能成为治疗胃癌的新途径,具有一定临床应用价值。

关键词：胃癌表观遗传学新途径

在线全文

学校读者我要写书评

暂无评论

乳腺干细胞标记物的研究进展

中国肿瘤临床 2007年第15期34卷 874-876页

作者：朱悦孙保存乳腺癌防治教育部重点实验室天津市"肿瘤防治"重点实验室天津医科大学附属肿瘤医院病理科天津市300060

乳腺干细胞不仅对乳腺的发生、分化和功能维持起到重要作用,而且近年来发现乳腺干细胞与乳腺癌的发生也有密切联系,有研究报道乳腺干细胞是乳腺癌发生的始动靶点,迄今为止,还没有明确的乳腺干细胞标记物研究报道,因此大多数学者都依靠... 详细信息

乳腺干细胞不仅对乳腺的发生、分化和功能维持起到重要作用,而且近年来发现乳腺干细胞与乳腺癌的发生也有密切联系,有研究报道乳腺干细胞是乳腺癌发生的始动靶点,迄今为止,还没有明确的乳腺干细胞标记物研究报道,因此大多数学者都依靠在造血、神经、表皮或其它系统中的干细胞标志去研究乳腺干细胞。本文对乳腺干细胞标记物的最新进展及其在乳腺癌发生中的作用作一综述。

关键词：乳腺干细胞标记物乳腺癌

在线全文

学校读者我要写书评

暂无评论

肿瘤干细胞与肿瘤血管异质性

中国肿瘤临床 2014年第2期41卷 134-137页

作者：刘志勇孙保存天津医科大学肿瘤医院肿瘤研究所病理科国家肿瘤临床医学研究中心天津市肿瘤实验重点实验室天津市300060

肿瘤血管新生是肿瘤发生和恶性演进过程中基本的生物学过程。传统的血管生成理论认为肿瘤血管新生主要通过由宿主血管网内皮细胞增殖、迁移长入肿瘤组织或由肿瘤组织募集骨髓中的内皮祖细胞增殖、分化为成熟内皮细胞以构建新生血管,即... 详细信息

肿瘤血管新生是肿瘤发生和恶性演进过程中基本的生物学过程。传统的血管生成理论认为肿瘤血管新生主要通过由宿主血管网内皮细胞增殖、迁移长入肿瘤组织或由肿瘤组织募集骨髓中的内皮祖细胞增殖、分化为成熟内皮细胞以构建新生血管,即内皮依赖性血管是肿瘤血供的唯一方式。然而越来越多的研究发现肿瘤的微循环网络是异质性的且肿瘤干细胞在肿瘤血管化过程中具有重要作用。本文就肿瘤干细胞(cancer stem cells,CSCs)对不同肿瘤血管新生模式的作用及其作为肿瘤抗血管生成疗法的潜在靶点做一概述,并对肿瘤干细胞促血管生成研究中待解决的问题进行了展望。

关键词：血管异质性肿瘤干细胞血管新生转分化血管生成拟态