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Randomized controlled trial of minimally invasive surgery using acellular dermal matrix for complex anorectal fistula

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World Journal of Gastroenterology 2010年第26期16卷 3279-3286页

作者： Ma-Mu-Ti-Jiang A ba-bai-ke-re Er-Ha-Ti Ai Anorectal Surgical Department First Aff iliated Hospital of Xinjiang Medical University

AIM: To compare the efficacy and safety of acellular dermal matrix (ADM) bioprosthetic material and endorectal advancement flap (ERAF) in treatment of complex anorectal fistula. METHODS: Ninety consecutive patients with complex anorectal fistulae admitted to Anorectal Surgical Department of First Affi liated Hospital, Xinjiang Medical university from March 2008 to July 2009, were enrolled in this study. Complex anorectal fistula was diagnosed following its clinical, radiographic, or endoscopic diagnostic criteria. under spinal anesthesia, patients underwent identification and irrigation of the fistula tracts using hydrogen peroxide. ADM was securely sutured at the secondary opening to the primary opening using absorbable suture. Outcomes of ADM and ERAF closure werecompared in terms of success rate, fecal incontinence rate, anorectal deformity rate, postoperative pain time, closure time and life quality score. Success was defined as closure of all external openings, absence of drainage without further intervention, and absence of abscess formation. Follow-up examination was performed 2 d, 2, 4, 6, 12 wk, and 5 mo after surgery, respectively. RESuLTS: No patient was lost to follow-up. The overall success rate was 82.22% (37/45) 5.7 mo after surgery. ADM dislodgement occured in 5 patients (11.11%), abscess formation was found in 1 patient, and fistula recurred in 2 patients. Of the 13 patients with recurrent fistula using ERAF, 5 (11.11%) received surgical drainage because of abscess formation. The success rate, postoperative pain time and closure time of ADM were significantly higher than those of ERAF (p uSION: Closure of fistula tract opening with ADM is an effective procedure for complex anorectal fistula. ADM should be considered a first line treatment for patients with complex anorectal fistula.

关键词： Acellular dermal matrix Surgery Transsphincteric complex fistula

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Cytochrome p450 2E1 RsaI/pstI and DraI polymorphisms Are Risk Factors for Lung Cancer in Mongolian and Han population in Inner Mongolia

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Chinese Journal of Cancer Research 2011年第2期23卷 107-111页

作者： Xiu-lan Su ba Bin Hong-wei Cui Mei-rong Ran Clinical Medical Center Affiliated Hospital Inner Mongolian Medical College Hohhot 010050 China Department of Internal Medicine Medical Emergency Center of Kangbashi New Direct Erdos City Inner Mongolia China

Objective： To explore the relationship between cytochrome p450 2E1 （CYp2E1） RsaI/pstI and DraI polymorphism and lung cancer susceptibility in Mongolian and Han population in Inner Mongolia of China. Methods： CYp2E1 RsaI/pstI and DraI polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism in 64 lung cancer patients, 150 healthy Mongolian and 150 healthy Han individuals. The distribution of genotype and allele frequencies of CYp2E1 RsaI/pstI and DraI polymorphisms were studied. Results： The risk of lung cancer was increased in individuals with CYp2E1 （cl/cl） and CYp2E1 （DD） with OR values of 2.431 （95%CI=1.082-5.460） and 2.778 （95%CI=1.358-5.683） respectively （p0.05）. When CYp2E1 RsaI/pstI and DraI polymorphisms were combined, the risk of lung cancer was reduced in individuals with CYp2E1 （cl/c2＋c2/c2 and DD＋CC） with OR values of 0.233 （95%CI=0.088-0.615, p0.05）. In smokers, the susceptibility to lung cancer was higher in the individuals with CYp2E1 （c1/c1） and CYp2E1 （DD） than in the individuals with c2 and C allele （p0.05, OR=2.643 and 4.308 respectively）. There was no significant difference in distribution of CYp2E1 genotype frequency between healthy Mongolian, Han population and lung cancer patients, healthy controls in Inner Mongolia. Conclusion： CYp2E1 （c1/c1） and CYp2E1 （DD） are predisposing factors of lung cancer in population in Inner Mongolia. CYp2E1 （c2﹢C） co-mutation may decrease the risk of lung cancer. Smoking exerts synergetic effect with CYp2E1 （c1/c1） and CYp2E1 （DD） on the occurrence of lung cancer.

关键词： Cytochrome p450 2E1 Gene polymorphism Lung cancer Susceptivity

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CSF Aβ1-42 level is associated with cognitive decline in early parkinson’s disease with rapid eye movement sleep behavior disorder

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Translational Neurodegeneration 2018年第1期7卷 212-220页

作者： Maowen ba Guoping Yu Min Kong Hui Liang Ling Yu Department of Neurology the Affiliated Yantai Yuhuangding Hospital of Qingdao UniversityYantai CityShandong 264000People’s Republic of China Department of Neurology Yantaishan HospitalYantai CityShandong 264000People’s Republic of China

background:Rapid eye movement sleep behavior disorder(RBD)is associated with cognitive decline in early parkinson’s disease(pD).However,the underlyling basis for this association remains ***:parkinson’s progression Marker’s Initiative(ppMI)subjects underwent baseline RBD testing with RBD sleep questionnaire(RBDSQ).Serial assessments included measures of motor symptoms,non-motor symptoms(NMS),neuropsychological assessment,blood and cerebrospinal fluid(CSF)*** to three years follow-up data were *** stratified early pD subjects into pD with RBD(RBDSQ score>5)and pD without RBD ***,we evaluated baseline biomarkers in each group as a predictor of cognitive decline using Montreal Cognitive Assessment(MoCA)score changes over three years in regression ***:Four hundred twenty-three pD subjects were enrolled at baseline,and a total of 350 pD subjects had completed 3 years of study follow-up with completely serial *** found that at baseline,only CSF β-amyloid 1–42(Aβ1–42)was significantly lower in pD subjects with *** three years follow-up analysis,pD subjects with RBD were more likely to develop incident mild cognitive impairment(MCI)and presented greater cognitive decline in MoCA *** baseline CSF Aβ1–42 predicted cognitive decline over 3 years only in pD subjects with RBD(β=−0.03,p=0.003).A significant interaction between Aβ1–42 and the 2 groups confirmed that this effect was indeed higher in pD with RBD than the other individual(β=−2.85,p=0.014).Conclusion:These findings indicate that CSF Aβ1–42 level is associated with global cognitive decline in early pD with *** addition of CSF Aβ1–42 to RBD testing increase the likelihood of identifying those at high risk for cognitive decline in early pD.

关键词： parkinson’s disease Rapid eye movement sleep behavior disorder Cognitive decline β-Amyloid

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636名海船船员听力调查结果分析

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中华航海医学与高气压医学杂志 2024年第4期31卷 447-449页

作者：易庆鹤丁猛董龙宝张小敏巴剑波海军军医大学海军特色医学中心耳鼻喉科上海200052 海军军医大学海军特色医学中心上海200433

目的调查分析海船船员听力损失情况,为航海职业防治噪声性耳聋提供参考依据。方法从某大型海船随机选取636名船员进行纯音听阈检查,分析听力损失情况并与同类研究结果进行比较。结果海船船员的听力损失检出率为22.33%(142/636),高频听... 详细信息

目的调查分析海船船员听力损失情况,为航海职业防治噪声性耳聋提供参考依据。方法从某大型海船随机选取636名船员进行纯音听阈检查,分析听力损失情况并与同类研究结果进行比较。结果海船船员的听力损失检出率为22.33%(142/636),高频听力损失的检出率高于语频听力损失,不同频率下听力损失程度差异无统计学意义(H=91.056,p>0.05);船员听力损失检出率低于以往海船船员的听力调查结果,差异均有统计学意义(p<0.05)。结论海船船员的听力损失情况得到改善,但噪声对船员听力的损害依然存在,建议有关部门采取多项措施预防船员发生噪声性耳聋。

关键词：船员噪声听力损失

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Cloning and expression of MXR7 gene in human HCC tissue

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World Journal of Gastroenterology 2000年第1期6卷 57-60页

作者： Zhou Xp Wang HY Yang GS Chen ZJ Li ba Wu MC The International Cooperation Laboratory on Signal Transduction Eastern Hepatobiliary Surgery Hospitalthe Second Military Medical UniversityShanghai 200438China

AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal *** The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? *** cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction（pCR）.Recombinant DNAconforming to reading frame was constructed byconnecting purified pCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion *** plasmid MXRT/pGEX-5X-1was identified by *** up>32up>p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues *** Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 *** blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,*** frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFp and in HCCpressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for *** detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFp.

关键词： Subject headings human HCC tissues MXR7 gene gene expression cDNA mRNA

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Effects of Carbonation on Micro Structures of Hardened Cement paste

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Journal of Wuhan university of Technology(Materials Science) 2016年第1期31卷 146-150页

作者：柳俊哲 yao senyuan ba mingfang he zhimin 李玉顺 Faculty of Architectural Civil Engineering and Environment Ningbo University Ningbo 315211 China

In order to investigate the effects of carbonation on the microstructure of cement concrete,the carbonation depth and microstructure of cement paste with 0.3,0.4 and 0.5 water/cement ratio after 7,14,21 and 28 d accelerated carbonation were studied *** results showed that with the increase of waterto-cement ratio and carbonation age,the carbonation depth was deepened with faster early carbonation speed and slower later carbonation *** densified the structure of hardened cement stone with refinement of pore structure and reduced ***,during the carbonation process from the surface to the inside of carbonation area,it was prone to form micro-cracks extending to the interior specimen,resulting in cement paste carbonation depth *** is further illustrated that the color reaction method using phenolphthalein solution combined with X-CT and X-ray diffraction analysis is much more reasonable to evaluate the cement concrete carbonation ***,during carbonation process sulfur element in cement paste migrated to the area un-carbonated and the concentrated shape of sulfur element is consistent with the coloring region in carbonation *** it was identified that carbonation not only reduced the p H value in cement concrete but also made prone to crack in carbonation zone,which increased the probability of reinforcement corrosion.

关键词： hardened cement paste carbonation sulfur microstructure X-CT analysis

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African Swine Fever Virus MGF360-12L Inhibits Type Ⅰ Interferon production by Blocking the Interaction of Importin α and NF-κB Signaling pathway

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Virologica Sinica 2021年第2期36卷 176-186页

作者： Yisha Zhuo Zeheng Guo Tongtong ba Cheng Zhang Lihua He Cuiping Zeng Hanchuan Dai College of Veterinary Medicine Huazhong Agricultural UniversityWuhan 430070China

African swine fever(ASF)is an infectious transboundary disease of domestic pigs and wild boar and spreading throughout *** is no vaccine and treatment *** immune escape strategies of African swine fever virus(ASFV)are crucial factors affecting immune prevention and vaccine ***360 genes have been implicated in the modulation of the IFN-Ⅰ*** molecular mechanisms contributing to innate immunity are poorly *** this study,we demonstrated that ASFV MGF360-12 L(MGF360 families 12 L protein)significantly inhibited the mRNA transcription and promoter activity of IFN-βand NF-κB,accompanied by decreases of IRF3,STING,TBK1,ISG54,ISG56 and Ap-1 m RNA ***,MGF360-12 L might suppress the nuclear localization of p50 and p65 mediated by classical nuclear localization signal(NLS).Additionally,MGF360-12 L could interact with KpNA2,KpNA3,and KpNA4,which interrupted the interaction between p65 and KpNA2,KpNA3,*** further found that MGF360-12 L could interfere with the NF-κB nuclear translocation by competitively inhibiting the interaction between NF-κB and nuclear transport *** findings suggested that MGF360-12 L could inhibit the IFN-Ⅰproduction by blocking the interaction of importinαand NF-κB signaling pathway,which might reveal a novel strategy for ASFV to escape the host innate immune response.

关键词： African swine fever virus(ASFV) MGF360-12L IFN-Ⅰ NF-κB Nuclear transport

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斑马鱼TYRp1b基因SNps筛查及其与体色性状的关联分析

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福建农业学报 2024年第4期39卷 387-397页

作者：宋兴超王云艳王莎莎方月巴家文梅杰梁正其铜仁学院农林工程与规划学院/贵州省梵净山地区生物多样性保护与利用重点实验室贵州铜仁554300

【目的】探究酪氨酸酶相关蛋白1b(tyrosinase-related protein 1b,TYRp1b)基因变异与斑马鱼体色性状的相关性。【方法】以体色表型存在显著差异的红色、黄色和蓝色斑马鱼共计219个样本的肌肉组织DNA为模板,设计3对引物,采用pCR扩增、San... 详细信息

【目的】探究酪氨酸酶相关蛋白1b(tyrosinase-related protein 1b,TYRp1b)基因变异与斑马鱼体色性状的相关性。【方法】以体色表型存在显著差异的红色、黄色和蓝色斑马鱼共计219个样本的肌肉组织DNA为模板,设计3对引物,采用pCR扩增、Sanger测序技术筛查TYRp1b基因单核苷酸多态性(single nucleotide polymorphisms,SNps),并将SNps与斑马鱼体色性状进行关联分析。【结果】获得的斑马鱼TYRp1b基因外显子2、部分内含子3、外显子4、内含子4、外显子5、内含子5、外显子6和外显子7长度分别为134、212、168、135、180、113、150、171 bp。SNps筛查显示,3种体色斑马鱼群体共筛查到18个SNps,部分内含子3存在11个SNps位点:g.2125G>A、g.2150G>A、g.2152G>T、g.2161C>A、g.2175A>T、g.2180G>T、g.2185A>T、g.2192A>C、g.2200A>T、g.2201A>C和g.2213T>C;内含子4检测到4个SNps位点:g.7C>A、g.65A>G、g.84T>A和g.103C>T;内含子5存在1个SNps位点:g.61G>T;外显子4、6分别存在1个SNps位点:g.125G>T和g.80T>A,且均为同义突变;外显子2、5和7均未检测到SNps。关联分析表明,TYRp1b基因内含子3中g.2152G>T、g.2175A>T、g.2180T>G、g.2192A>C、g.2200A>T、g.2201A>C位点、内含子4中g.65A>G位点及外显子6中g.80T>A位点的基因型均与斑马鱼体色性状极显著相关(pp1b基因内含子3、4及外显子6存在8个与体色表型存在关联的SNps,可能影响斑马鱼体色性状或与之紧密连锁。

关键词：斑马鱼酪氨酸酶相关蛋白1b基因单核苷酸多态性体色性状关联分析

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BDNF多态性在氟暴露与儿童ACTH/CORT关联中的作用

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中华地方病学杂志 2024年第9期43卷 689-697页

作者：李娜冯子臣周郭育王安琪丁丽敏余方方巴月郑州卫生健康职业学院膳食中心郑州郑州大学公共卫生学院环境卫生学教研室郑州河南省疾病预防控制中心郑州

目的探讨豫东农村儿童氟暴露与促肾上腺皮质激素(adrenocorticotropic hormone, ACTH)和皮质醇(cortisol, CORT)的关系, 揭示脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)基因多态性在其中的修饰效应。方法采用整群抽... 详细信息

目的探讨豫东农村儿童氟暴露与促肾上腺皮质激素(adrenocorticotropic hormone, ACTH)和皮质醇(cortisol, CORT)的关系, 揭示脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)基因多态性在其中的修饰效应。方法采用整群抽样方法, 于河南省通许县4所小学招募7 ～ 12岁儿童463名(男童245名、女童218名), 对其进行问卷调查、体格检查以及晨尿、空腹静脉血采集。分别应用氟离子选择电极法和苦味酸法测定尿氟及尿肌酐浓度;结合全自动生化分析仪测定血清ACTH及CORT水平;采用定制的48-plex SNpscanTM试剂盒对BDNF基因单核苷酸多态性位点进行基因分型。使用多元线性回归分析尿氟浓度与儿童血清ACTH或CORT水平的关系;同时建立尿氟浓度与BDNF基因多态性的交互项, 使用多元线性回归分析单位点基因多态性与环境对儿童血清ACTH或CORT水平的交互作用。结果尿氟浓度每升高1 mg/L, 女童血清ACTH水平增加1.98 pg/ml[95%置信区间(CI):0.71, 3.24;p = 0.002]、男童血清CORT水平降低37.48 ng/ml(95%CI:- 63.99, - 10.97;p = 0.006)。无论是否分层分析, 携带rs6484320位点TA基因型者尿氟浓度均与血清ACTH水平呈正相关(均β > 0, 均p 0, 均p 0, 均p p交互 p交互 = 0.035)。结论尿氟浓度与女童血清ACTH水平升高及男童血清CORT水平降低有关;BDNF基因多态性可修饰氟暴露与儿童血清ACTH或CORT水平的关联, 且修饰效应存在性别差异。

关键词：氟儿童脑源性神经营养因子单核苷酸多态性促肾上腺皮质激素皮质醇

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复合植物多糖对肉兔生长性能、养分表观消化率和血清指标的影响

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饲料研究 2024年第5期47卷 48-52页

作者：张风荣于枫王赠超王辰姜八一徐海滨山东畜牧兽医职业学院山东潍坊261061

试验旨在研究复合植物多糖对肉兔生长性能、养分表观消化率和血清指标的影响。将120只肉兔随机分为4组,每组15个重复,每个重复2只肉兔。对照组饲喂基础饲粮,复合植物多糖Ⅰ组、复合植物多糖Ⅱ组、复合植物多糖Ⅲ组分别在基础饲粮中添加0... 详细信息

试验旨在研究复合植物多糖对肉兔生长性能、养分表观消化率和血清指标的影响。将120只肉兔随机分为4组,每组15个重复,每个重复2只肉兔。对照组饲喂基础饲粮,复合植物多糖Ⅰ组、复合植物多糖Ⅱ组、复合植物多糖Ⅲ组分别在基础饲粮中添加0.01%、0.05%、0.10%复合植物多糖。试验期35 d。结果显示,3个复合植物多糖添加组的末重、平均日增重均显著高于对照组(ppp)、中性洗涤纤维(NDF)、粗脂肪(EE)、粗纤维(CF)、酸性洗涤纤维(ADF)表观消化率均显著高于对照组(pp)、白蛋白(ALB)、免疫球蛋白G (IgG)、免疫球蛋白A (IgA)、白细胞介素-2 (IL-2)的含量及总抗氧化能力(T-AOC)均显著高于对照组(puN)含量显著低于对照组(pu)、免疫球蛋白M (IgM)、白细胞介素-6 (IL-6)的含量和超氧化物歧化酶(SOD)活性均显著高于对照组(pp<0.05)。研究表明,饲粮中添加复合植物多糖能够改善肉兔的生长性能、养分表观消化率和血清指标。在肉兔养殖中,选择0.05%复合植物多糖效果较好。

关键词：复合植物多糖肉兔生长性能养分表观消化率免疫指标抗氧化指标

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