Cloning and expression of MXR7 gene in human HCC tissue

作     者：Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC 

作者机构：The International Cooperation Laboratory on Signal TransductionEastern Hepatobiliary Surgery Hospitalthe Second Military Medical UniversityShanghai 200438China 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2000年第6卷第1期

页      面：57-60页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Foundation of China,No.39770379 the National Basic Research Project("973") SUGEN,USA 

主　　题：Subject headings human HCC tissues MXR7 gene gene expression cDNA mRNA 

摘      要：AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal *** The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? *** cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction（PCR）.Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion *** plasmid MXRT/pGEX-5X-1was identified by *** 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues *** Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 *** blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,*** frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC5cm was 90%（9/10）and 83.3%（5/6）,*** MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for *** detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP.