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Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings

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Journal of Forestry Research 2022年第5期33卷 1573-1584页

作者： Leandro Silva de Oliveira Gilvano Ebling Brondani Letícia Vaz Molinari Rafaella Zanetti Dias Gustavo Leal Teixeira antônio natal gonçalves Marcílio de Almeida Institute of Agricultural Science Federal University of Minas GeraisUniversity Campus1000Universitária Ave.Montes ClarosMG 39404-547Brazil Laboratory of In Vitro Culture of Forest Species Department of Forestry SciencesFederal University of LavrasUniversity CampusCentral AvenueLavrasMG 37200-900Brazil Department of Biological Sciences “Luiz de Queiroz”College of AgricultureUniversity of São Paulo(ESALQ/USP)University Campus11Pádua Dias AvenuePiracicabaSP 13418-900Brazil

Shoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana *** enables large-scale production of plants from transformed *** study evaluates the effect ofα-naphthaleneacetic acid(NAA),thidiazuron(TDZ)and benzylaminopurine(BAP)on the organogenesis of *** from hypocotyls and cotyledonary *** the induction stage,hypocotyls and cotyledonary leaves were established in a Murashige and Skoog(MS)culture medium supplemented with NAA or *** tissues were cultivated in a MS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation *** buds were multiplied in vitro and elongated in a WPM culture medium supplemented with 0.89μM BAP and 0.05μM *** leaves exhibited the best in vitro *** induction of adventitious buds occurred only in calluses induced from *** the differentiation stage,4.4μM BAP treatment promoted an increase of adventitious bud ***-cuttings from regenerated shoots were acclimatized and rooted ex vitro in *** results confirm the establishment of an efficient protocol for the in vitro regeneration of *** by indirect organogenesis,providing new insights regarding cloning of this species.

关键词： Micropropagation Recalcitrant species Plant growth regulator Explants source

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In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides

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Journal of Forestry Research 2011年第2期22卷 209-215页

作者： Yurika H.Komatsu Katherine Derlene Batagin-Piotto Gilvano Ebling Brondani antnio natal gonalves Marcílio de Almeida Graduate Program in Forest Resources Department of Forest Sciences"Luiz de Queiroz" College of AgricultureUniversity of So Paulo(ESALQ/USP)Avenida Pádua Dias11PiracicabaSo PauloBrazil Graduate Program in Biochemistry of Plants Department of Biological Sciences"Luiz de Queiroz" College of AgricultureUniversity of So Paulo(ESALQ/USP)Avenida Pádua Dias11PiracicabaSo Paulo

Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram（4-amino-3,5,6-trichloropicolinic acid） at different concentrations of carbohydrate under the same conditions with presence or absence of *** our experiment,secondary explants were kept in MS medium containing 8.0 mg·L-1 of Picloram for the callus *** were transferred in MS medium supplemented with sucrose,fructose and glucose（control,2%,4% and 6%）.Results show that Picloram induced the callogenesis in leaf *** secondary embryogenesis was formed in yellow-globular *** sucrose as carbohydrate source in the absence of light was more efficient to induce *** was more efficiency in the presence of *** induction and further embryogenesis evidenced the competence and determination of leaf sheath cells.

关键词： poaceae tissue culture callus induction cell competence determination

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Low temperature,IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings

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Journal of Forestry Research 2012年第4期23卷 583-592页

作者： Gilvano Ebling Brondani Francisco JoséBenedini Baccarin Heron Wilhelmus de Wit Ondas JoséLuiz Stape antonio natal gonçalves Marcilio de Almeida Department of Forest Engineering Federal University of Mato Grosso(UFMT)Avenida Fernando Corrêa da Costa2367ZC 78060-900CuiabáMato GrossoBrazil. Department of Forest Sciences "Luiz de Queiroz"College of AgricultureUniversity of S o Paulo(ESALQ/USP)Avenida Pádua Dias11POB 9ZC 13418-900PiracicabaSo PauloBrazil. Department of Biological Sciences "Luiz de Queiroz"College of AgricultureUniversity of S o Paulo(ESALQ/USP)Avenida Pádua Dias11POB 9ZC 13418-900PiracicabaSo PauloBrazil. Department of Forest and Environmental Sciences North Carolina State UniversityNC 27695USA.

Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting or mini-cutting. We aimed to assess the adventitious rooting percentage under different storage times in low temperatures and at various IBA （indole-3-butyric acid） concentrations to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse. Shoots collected from mini-stumps cultivated in a semi-hydroponic system were used to obtain the mini-cuttings. For the first experiment, the mini-cuttings were stored at 4℃ for 0 （immediate planting）, 24, 48, 72, 96 and 120 h. The second experiment evaluated the rooting dynamic to determine the optimal time of permanence for minicuttings in a greenhouse. The basal region of the mini-cutting was treated with various IBA solutions： 0 （free of IBA）, 1,000, 2,000, 3,000 and 4,000 mg.L^-1. Every seven days （0 （immediate planting）, 7, 14, 21 and 28 days）, destructive sampling of the mini-cuttings was performed to evaluate the histology of the adventitious rooting. Eucalyptus benthamii minicuttings should be rooted immediately after the collection of the shoots. The 2,000 mg.L^-1 IBA concentration induced a greater speed and percentage of adventitious rooting, and an interval of 35 to 42 days was indicated for permanence of the mini-cuttings in the greenhouse. Expo- sure to low temperature induced adventitious root formation with diffuse vascular connections.

关键词： rhizogenesis plant cloning mini-cutting technique histological analysis indole-3-butyric acid.

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Turning Industrial Waste into a Valuable Bioproduct:Starch from Mango Kernel Derivative to Oil Industry Mango Starch Derivative in Oil Industry

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Journal of Renewable Materials 2019年第2期7卷 139-152页

作者： Nívia do Nascimento Marques Caroline Suzy do Nascimento Garcia Liszt Yeltsin Coutinho Madruga Marcos antônio Villetti Men de SáMoreira de Souza Filho Edson Noriyuki Ito Rosangela de Carvalho Balaban Instituto de Química Universidade Federal do Rio Grande do Norte-UFRNNatal59078970Brazil Departamento de Física Universidade Federal de Santa Maria-UFSMSanta Maria97105900Brazil Embrapa Agroindústria Tropical Fortaleza60511-110Brazil Departamento de Engenharia de Materiais Universidade Federal do Rio Grande do Norte-UFRNNatal59078970Brazil

After industrial mango processing,tons of residues such as peels and kernels are discarded as ***,almost 60% of the mango kernel is due to starch on a dry weight ***,starch from mango(Manguifera Indica L.)kernel was applied to obtain a starch fatty ester with vinyl laurate,in DMSO,under basic ***,1H and 13C NMR confirmed that a starch ester with a degree of modification of 2.6 was *** showed that the modified starch has higher thermal stability than its precursors and higher than a vinyl laurate/starch physical *** data showed that granular shape and smooth surface on mango starch changed after chemical modification to a continuous and shapeless *** industrial reject derivative behaved as an efficient alternative environmentally friendly fluid loss controller in oil drilling fluids,even in conditions of high temperatures and high pressures(HTHP)drilling.

关键词： Mango Starch oil drilling fluid transesterification vinyl laurate

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Analysis of antisym metric cross-ply laminates using high-order shear deformation theories:a meshless approach

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Acta Mechanica Sinica 2020年第5期36卷 1078-1098,I0003页

作者： D.E.S.Rodrigues J.Belinha L.M.J.S.Dinis R.M.natal Jorge Institute of Science and Innovation in Mechanical and Industrial Engineering 4200-465 PortoPortugal Department of Mechanical Engineering School of EngineeringPolytechnic of Porto(ISEP)4200-072 PortoPortugal Department of Mechanical Engineering Faculty of EngineeringUniversity of Porto(FEUP)4200-465 PortoPortugal

For many years finite element method(FEM)was the chosen numerical method for the analysis of composite ***,in the last 20 years,the scientific community has witnessed the birth and development of several meshless methods,which are more flexible and equally accurate numerical *** meshless method used in this work is the natural neighbour radial point interpolation method(NNRPIM).In order to discretize the problem domain,the NNRPIM only requires an unstructured nodal ***,using the Voronoi mathematical concept,it enforces the nodal connectivity and constructs the background integration *** NNRPIM shape functions are constructed using the radial point interpolation *** this work,the displacement field of composite laminated plates is defined by high-order shear deformation *** the end,several antisymmetric cross-ply laminates were analysed and the NNRPIM solutions were compared with the *** obtained results show the efficiency and accuracy of the NNRPIM formulation.

关键词： antisymmetric cross-ply laminates High-order shear deformation theories Meshless methods Naturalneighbour radial point interpolation method(NNRPIM)

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High chromosomal evolutionary dynamics in sleeper gobies(Eleotridae)and notes on disruptive biological factors in Gobiiformes karyotypes(Osteichthyes,Teleostei)

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Marine Life Science & Technology 2021年第3期3卷 293-302页

作者： Simião Alefe Soares da Silva Paulo Augusto de Lima-Filho Clóvis Coutinho da Motta-Neto Gideão Wagner Werneck Félix da Costa Marcelo de Bello Cioffi Luiz antônio Carlos Bertollo Wagner Franco Molina Departamento de Biologia Celular e Genética Centro de BiociênciasUniversidade Federal do Rio Grande do NorteNatalRN59.078-970Brazil Instituto Federal de Ciência e Tecnologia do Rio Grande do Norte Santa CruzRN59200-000Brazil Departamento de Genética e Evolução Universidade Federal de São CarlosRodovia Washington LuísKm 235São CarlosSP13.565-905Brazil

The order Gobiiformes is made up of more than 2200 species,representing one of the most diverse groups among teleost *** biological causes for the tachytelic karyotype evolution of the gobies have not yet been fully *** we expanded cytogenetic data for the Eleotridae family,analyzing the neotropical species Dormitator maculatus,Eleotris pisonis,Erotelis smaragdus,and Guavina *** addition,a meta-analytical approach was followed for elucidating the karyotype diversification versus biological aspects(habitat and egg type)of the *** species *** and *** present 2n=46 acrocentric chromosomes(NF=46),*** 2n=46(36sm+4st+6a;NF=86),and ***,the most divergent karyotype,with 2n=52 acrocentric chromosomes(NF=52).Besides numeric and structural diversification in the karyotypes,the mapping of rDNAs and microsatellites also showed noticeable numerical and positional variation,supporting the high chromosomal evolutionary dynamism of these *** Gobiiformes,karyotype patterns which are more divergent from the basal karyotype(2n=46a)are associated with characteristics less effective to dispersion,such as the benthic *** adaptive characteristics,connected with the organization of the repetitive DNA content in the chromosomes,likely play a synergistic role in the remarkable karyotype diversification of this group.

关键词： Chromosome evolution Dispersive potential Goby Karyotype diversification Microsatellites rDNA

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In vitro adherence of conjunctival bacteria to different oculoplastic materials

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International Journal of Ophthalmology(English edition) 2018年第12期11卷 1895-1901页

作者： Alvaro Toribio Honorina Martinez-Blanco Leandro Rodriguez-Aparicio Miguel A.Ferrero Teresa Marrodán Isabel Fernández-natal Department of Ophthalmology University Hospital of Leon Leon 24071Spain Department of Molecular Biology University of LeonLeon 24071Spain Department of Clinical Microbiology University Hospital of LeonLeon 24071Spain

AIM: To investigate the resistance to bacterial adhesion of materials used in oculoplastic surgery, particularly materials used in the manufacture of orbital ***: Seven organisms of conjunctival flora(two strains of Staphylococcus epidermidis and one strain each of Staphylococcus aureus, Staphylococcus hominis, Corynebacterium amycolatum, Acinetobacter calcoaceticus, and Serratia marcescens) were selected. A lactic acid bacterium(Lactobacillus rhamnosus) was also included as positive control because of its well-known adhesion ability. Eight materials used to make oculoplastic prostheses were selected(glass, steel, polytetrafluoroethylene, polymethylmethacrylate, silicone from orbital implants, commercial silicone, porous polyethylene, and semismooth polyethylene). Materials surfaces and biofilms developed by strains were observed by scanning electron microscopy. Kinetics of growth and adhesion of bacterial strains were determined by spectrophotometry. Each strain was incubated in contact with plates of the different materials. After growth, attached bacteria were re-suspended and colony-forming units(CFUs) were counted. The number of CFUs per square millimetre of material was statistically ***: A mature biofilm was observed in studied strains except Staphylococcus hominis, which simply produced a microcolony. Materials showed a smooth surface on the microbial scale, although steel exhibited 1.0-μm-diameter grooves. Most organisms showed significant differences in adhesion according to the material. There were also significant differences in thetotal number of CFUs per square millimetre from each material(P=0.044). CFU counts were significantly higher in porous polyethylene than in silicone from orbital implants(P=0.038).CONCLUSION: Silicone orbital implants can resist microbial colonization better than porous polyethylene implants.

关键词： conjunctival flora microbial adhesion biofilm orbital implant oculoplastic prosthesis

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Conjunctival flora in anophthalmic patients: microbiological spectrum and antibiotic sensitivity

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International Journal of Ophthalmology(English edition) 2019年第5期12卷 765-773页

作者： Alvaro Toribio Teresa Marrodán Isabel Fernández-natal Honorina Martínez-Blanco Leandro Rodríguez-Aparicio Miguel á.Ferrero Department of Ophthalmology University Hospital of León Department of Clinical Microbiology University Hospital of León Department of Molecular Biology University of León

AIM: To identify the spectrum and susceptibility pattern of isolated microorganisms from conjunctival flora of anophthalmic ***: A cross-sectional clinical study including 60 patients with unilateral anophthalmia. Patients with use of antibiotic drops in their socket during the last month were also included. From each patient, three microbiological samples were taken from the lower conjunctival sac(healthy eye, pre-prosthesis, and retro-prosthesis space of socket). The 180 samples obtained were cultured. Isolates were identified and their antibiotic sensitivities were ***: A total of 251 isolates were recovered(62 isolates from healthy eye, 93 from pre-prosthesis, and 96 from retro-prosthesis space). The most common organism was Staphylococcus epidermidis, in both healthy eyes(64.5%) and sockets(45.5%). Altogether, coagulasepositive Staphylococci, Streptococci, and Gram-negative bacteria accounted for less than 15% of isolates in healthy eyes and more than 35% in sockets. Regarding the antibiotic sensitivities, there were no significant differences between isolates from sockets and healthy eyes. Nine patients recognized the use of self-prescribed antibiotic drops in their socket. In the healthy eyes of these subjects, Gram-positive microorganisms showed significantly greater resistance to aminoglycosides and ***: Sockets of anophthalmic patients show a greater number of pathogens compared to healthy *** use of antibiotic drops in the socket promotes a resistant flora not only in the socket but also in the healthy eye. Quinolones and macrolides may be better therapeutic options than aminoglycosides for treating conjunctivitis of anophthalmic sockets, since these antibiotics are less active against Staphylococcus epidermidis.

关键词： socket ocular prosthesis antibiotic resistance microflora conjunctival dysbiosis

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Traits uncover quasi-neutral community assembly in a coastal heath vegetation

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Journal of Plant Ecology 2019年第4期12卷 703-712页

作者： JoséLuiz alves Silva Alexandre Fadigas Souza Louis Stephen Santiago Programa de Pós-Graduação em Ecologia Departamento de EcologiaUniversidade Federal do Rio Grande do NorteNatal 59078-970Brazil Departamento de Ecologia Universidade Federal do Rio Grande do NorteNatal 59078-970Brazil Department of Botany and Plant Sciences University of California2150 Batchelor HallRiversideCA 92521USA Smithsonian Tropical Research Institute BalboaAncónPanamáRepublic of Panamá

Aims Trait-based approaches are increasingly being used in ecology due to their potential to explain and predict community structure and dynamic regardless of species ***,we asked whether coastal heath vegetation established in stressful habitats could be assembled by quasi-neutral mechanisms based on functional trait *** are organized through quasi-neutral dynamics if abiotic gradients and/or species interactions impose sorting on functional traits and species performances,but not on species phylogenetic relatedness and *** The study was conducted in a coastal area containing tall dunes near the sea,and relatively flat areas punctuated by short palaeodunes further inland,Northeastern South *** evaluated 10 traits of the 21 most abundant species,as well as eight abiotic variables in 70 plots(25 m^(2))established in dune tops,valleys,flat and steep *** effect of environmental filter was tested by linear mixed models(LMM)between each community-weighted mean(CWM)trait and the abiotic variables,and by two indices of environmental filtering based on intra-and interspecific-trait vari-ability relative to null *** evaluate the effect of species inter-actions,we used an index of niche overlap relative to null models,and LMMs between this index and the species richness of *** Findings The environmental filtering index did not deviate from null expect-ations for any *** that included individual variability per-formed similarly to models that used species mean ***,the explicit consideration of environmental gradients by CWM ana-lyses revealed functional patterns that would remain undetected if trait distribution was analyzed *** addition,intraspecific vari-ation in the stem length was an important driver of species diversity as suggested by the relationship between species richness and the niche overlap *** on the occurrence and phylogenetic re-latedness o

关键词： Restinga heath vegetation harsh environment stress-tolerant species response traits

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Impact of the metabolic syndrome on the evolution of neurodegenerative diseases

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Neural Regeneration Research 2021年第4期16卷 688-689页

作者： Ana Paula de A.Boleti Jeeser alves Almeida Ludovico Migliolo S-InovaBiotech Programa de Pós-Graduação em BiotecnologiaUniversidade Católica Dom BoscoCampo GrandeMSBrazil Programa de Pós-Graduação em Ciências do Movimento Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro OesteUniversidade Federal de Mato Grosso do SulCampo GrandeMSBrazil Programa de Pós-graduação em Biologia Celular e Molecular Programa de Pós-graduação em BioquímicaUniversidade Federal do Rio Grande do NorteNatalBrazil

Metabolic syndrome(MetS)might be defined as the simultaneous accumulation of s everal functional changes that frequently occur in adults over 60 years of age(Gomez et al.,2018).The diagnosis of MetS requires the presence of three or more factors such as high body mass,type-2 diabetes mellitus(T2DM),dyslipidemia,and arterial hypertension,which increase the risk of cardiovascular diseases as well as neurological complications,as stroke and dementia(Dyken and Lacoste,2018).Usually,these functional changes coincide and result in insensitivity for example hormones as leptin,adiponectin,and insulin(Dyken and Lacoste,2018).

关键词： diagnosis arterial hypertension

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