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A prospective randomized study to define the role of low dose continuous prophylactic antibiotics and anti-adherence agents in altering the microbial colonization related to indwelling double‐J stents

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Asian Journal of Urology 2021年第3期8卷 269-274页

作者： kumar Madhavan sanchit Rustagi Rahul Jena Uday Pratap singh M.s.Ansari Aneesh srivastava Rakesh Kapoor sanjoy kumar sureka Department of Urology and Renal Transplantation SGPGIMSLucknowIndia

Objective:Despite conflicting evidence,it is common practice to use continuous antibiotic prophylaxis(CAP)in patients with indwelling double-J(DJ)*** extracts and d-mannose have been shown to prevent colonization of the urinary *** evaluated their role in this ***:We conducted a prospective randomized study to evaluate patients with indwelling DJ stents following urological *** were randomized into three *** A(n=46)received CAP(nitrofurantoin 100 mg once daily[od]).Group B(n=48)received cranberry extract 300 mg and d-mannose 600 mg twice daily(BD).Group C(n=40)received no *** stents were removed between 15 days and 45 days after *** groups were compared in terms of colonization of stent and urine,stent related symptoms and febrile urinary tract infections(UTIs)during the period of indwelling stent and until 1 week after ***:In Group A,9(19.5%)patients had significant bacterial growth on the *** was 8(16.7%)in the Group B and 5(12.5%)in Group C(p-value:0.743).However,the culture positivity rate of urine specimens showed a significant difference(p-value:0.023)with Group B showing least colonization of urine compared to groups A and *** was no statistically significant difference in the frequency of stent related symptoms(p-value:0.242)or febrile UTIs(p-value:0.399)among the ***:Prophylactic agents have no role in altering bacterial growth on temporary indwelling DJ stent,stent related symptoms or febrile *** extract may reduce the colonization of urinary tract,but its clinical significance needs further evaluation.

关键词： Antibiotic Prophylaxis stent Cranberry Infection

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Enhanced Detection of Glaucoma on Ensemble Convolutional Neural Network for Clinical Informatics

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Computers, Materials & Continua 2022年第2期70卷 2563-2579页

作者： D.stalin David s.Arun Mozhi selvi s.sivaprakash P.Vishnu Raja Dilip kumar sharma Pankaj Dadheech sudhakar sengan Department of Computer science and Engineering IFET College of EngineeringVillupuram605108Tamil NaduIndia Department of Computer science Engineering DMI St.John the Baptist UniversityMangochiLilongweMalawi Department of Information Technology CMR Engineering College(Autonomous)Hyderabad501401TelanganaIndia Department of Computer science and Engineering Kongu Engineering CollegePerundurai638060Tamil NaduIndia Department of Mathematics Jaypee University of Engineering and TechnologyGuna473226Madhya PradeshIndia Department of Computer science and Engineering Swami Keshvanand Institute of TechnologyManagement&Gramothan(SKIT)Jaipur302017RajasthanIndia Department of Computer science and Engineering PSN College of Engineering and TechnologyTirunelveli627152Tamil NaduIndia

Irretrievable loss of vision is the predominant result of Glaucoma in the ***,multiple approaches have paid attention to the automatic detection of glaucoma on fundus *** to the interlace of blood vessels and the herculean task involved in glaucoma detection,the exactly affected site of the optic disc of whether small or big size cup,is deemed *** Based Ellipse Fitting Curve Model(sBEFCM)classification is suggested based on the Ensemble for a reliable diagnosis of Glaucomain theOptic Cup(OC)and Optic Disc(od)boundary *** research deploys the Ensemble Convolutional Neural Network(CNN)classification for classifying Glaucoma or Diabetes Retinopathy(DR).The detection of the boundary between the OC and the od is performed by the sBEFCM,which is the latest weighted ellipse fitting *** sBEFCM that enhances and widens the multi-ellipse fitting technique is proposed *** is a preprocessing of input fundus image besides segmentation of blood vessels to avoid interlacing surrounding tissues and blood *** ascertaining of OCandodboundary,which characterizedmany output factors for glaucoma detection,has been developed by EnsembleCNNclassification,which includes detecting sensitivity,specificity,precision,andArea Under the receiver operating characteristic Curve(AUC)values accurately by an innovative *** terms of contrast,the proposed Ensemble CNNsignificantly outperformed the current methods.

关键词： Glaucoma and diabetic retinopathy detection ensemble convolutional neural network spatially based ellipse fitting curve optic disk optic cup

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Dichlorodiphenyltrichloroethane (DDT) and its metabolites in dairy milk and milk products of Agra City, India

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Chinese Journal Of Geochemistry 2006年第B8期25卷 179-179页

作者： anoop kumar PE Joseph Department of Chemistry St. John's College Agra India

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Multilocus phylogeny suggests a distinct species status for the Nepal population of Assam macaques(Macaca assamensis):implications for evolution and conservation

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Zoological Research 2021年第1期42卷 3-13页

作者： Laxman Khanal Mukesh kumar Chalise Peng-Fei Fan Randall CKyes Xue-Long Jiang Central Department of Zoology Institute of Science and TechnologyTribhuvan UniversityKathmandu 44613Nepal state Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650203China Nepal Biodiversity Research society(NEBORs) Lalitpur 23513Nepal school of Life sciences Sun Yat-sen UniversityGuangzhouGuangdong 510275China Department of Psychology Global Healthand AnthropologyCenter for Global Field Studyand Washington National Primate Research CenterUniversity of WashingtonSeattle 98195USA

Phylogenetic relationships within the sinica-group of macaques based on morphological,behavioral,and molecular characteristics have remained *** Nepal population of Assam macaques(Macaca assamensis)(NPAM),the westernmost population of the species,is morphologically distinct but has never been used in phylogenetic ***,the phylogenetic relationship of NPAM with other congeners was tested using multiple mitochondrial and Ychromosomal *** divergence times and evolutionary genetic distances among macaques were also *** revealed two major mitochondrial DNA clades of macaques under the sinica-group:the first clade included ***,***,and eastern subspecies of Assam macaque(*** assamensis);the second clade included *** together with species from the eastern and central Himalaya,namely,***,***,and *** the second-clade species,NPAM was the first to diverge from the other members of the clade around 1.9 million years *** results revealed that NPAM is phylogenetically distinct from the eastern Assam macaques and closer to other species and hence may represent a separate *** of its phylogenetic distinctiveness,isolated distribution,and small population size,the Nepal population of sinica-group macaques warrants detailed taxonomic revision and high conservation priority.

关键词： Himalaya Macaques Paraphyletic sinica-group Taxonomy

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Common polymorphisms of protein tyrosine phosphate non-receptor type 2 gene are not associated with risk of Crohn’s disease in Indian

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World Journal of Gastrointestinal Pathophysiology 2022年第4期13卷 114-123页

作者： Kaushik Chatterjee Amit kumar Dutta Ashish Goel Rekha Aaron Vijayalekshmi Balakrishnan Ajith Thomas anoop John Rajeeb Jaleel Deepu David Reuben Thomas Kurien sD Chowdhury Ebby George simon AJ Joseph Prasanna Premkumar Anna B Pulimood Department of Gastrointestinal sciences Christian Medical College and HospitalVellore 632004Tamil NaduIndia Department of Gastroenterology Christian Medical CollegeVellore 632004Tamil NaduIndia Departments of Biostatistics Christian Medical CollegeVellore 632004Tamil NaduIndia

BACKGROUND Multiple genetic risk factors for Crohn’s disease(CD)have been ***,these observations are not consistent across different *** protein tyrosine phosphate non-receptor type 2(PTPN2)gene plays a role in various aspects of host defense including epithelial barrier function,autophagy,and innate and adaptive immune *** common polymorphisms in the PTPN2 gene(rs2542151 and rs7234029)have been associated with risk of CD in Western *** To evaluate the association of PTPN2 gene polymorphisms with risk of CD in Indian *** We conducted a prospective case-control *** with CD were recruited,and their clinical and investigation details were *** were patients without organic gastrointestinal disease or other comorbid *** common polymorphisms in the PTPN2 gene(rs2542151 and rs7234029)were *** was extracted from peripheral blood samples of cases and controls and target DNA was amplified using specific sets of *** amplified fragments were digested with restriction enzymes and the presence of polymorphism was detected by restriction fragment length *** frequency of alleles was *** frequencies of genotypes and alleles were compared between cases and controls to look for significant *** A total of 108 patients with CD(mean age 37.5±12.7 years,females 42.6%)and 100 controls(mean age 39.9±13.5 years,females 37%)were *** the single nucleotide polymorphism(sNP)rs7234029,the overall frequency of G variant genotype(AG or GG)was noted to be significantly lower in the cases compared to controls(35.2%vs 50%,P=0.05).For the sNP rs2542151,the overall frequency of G variant genotype(GT or GG)was noted to be similar in cases compared to controls(43.6%vs 47%,P=0.73).There were no significant differences in minor allele(G)frequency for both polymorphisms between the cases and *** the sNPs had no significant association with a

关键词： PTPN2 gene Crohn’s disease Genetic polymorphism Case-control study Asia Risk factor

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Dual role of lipids for genome stability and pluripotency facilitates full potency of mouse embryonic stem cells

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蛋白质与细胞:英文版 2023年第8期14卷 591-602页

作者： Liangwen Zhong Miriam Gordillo Xingyi Wang Yiren Qin Yuanyuan Huang Alexey soshnev Ritu kumar Gouri Nanjangud Daylon James C.David Allis Todd Evans Bryce Carey Duancheng Wen Department of Reproductive Medicine Ronald O.Perelman and Claudia Cohen Center for Reproductive MedicineWeill Cornell MedicineNew YorkNY 10065USA Department of surgery Weill Cornell Medical College1300 York AvenueNew YorkNY 10065USA Laboratory of Chromatin Biology and Epigenetics The Rockefeller UniversityNew YorkNY 10065USA Cold spring Harbor Laboratory Cold Spring HarborNY 11724USA Molecular Cytogenetics Core.Memorial sloan Kettering Cancer Center New YorkNY 10065USA Department of Neuroscience Developmental and Regenerative BiologyUniversity of Texas at San AntonioOne UTSA CircleSan AntonioTX 78249USA Gladstone Institutes 1650 Owens StSan FranciscoCA94158USA

While Mek1/2 and Gsk3βinhibition("2i")supports the maintenance of murine embryonic stem cells(EsCs)in a homogenous naive state,prolonged culture in 2i results in aneuploidy and DNA hypomethylation that impairs developmental ***,2i fails to support derivation and culture of fully potent female *** we find that mouse EsCs cultured in 2i/LIF supplemented with lipid-rich albumin(AlbuMAx)undergo pluripotency transition yet maintain genomic stability and full potency over long-term ***,lipids in AlbuMAx impact intracellular metabolism including nucleotide biosynthesis,lipid biogenesis,and TCA cycle intermediates,with enhanced expression of DNMT3s that prevent DNA *** induce a formative-like pluripotent state through direct stimulation of Erk2 phosphorylation,which also alleviates X chromosome loss in female ***,both male and female"all-Esc"mice can be generated from de novo derived EsCs using AlbuMAXbased *** findings underscore the importance of lipids to pluripotency and link nutrient cues to genome integrity in early development.

关键词： mouse pluripotent stem cells lipids pluripotency transition genomic stability developmental potency nucleotide pool depletion 2i medium X chromosome loss femaleall-EsC mice

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Interleukin-6 mediates neutrophil mobilization from bone marrow in pulmonary hypertension

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Cellular & Molecular Immunology 2021年第2期18卷 374-384页

作者： Jonathan Florentin Jingsi Zhao Yi-Yin Tai sathish Babu Vasamsetti scott P.O’Neil Rahul kumar Anagha Arunkumar Annie Watson John sembrat Grant C.Bullock Linda sanders Biruk Kassa Mauricio Rojas Brian B.Graham stephen Y.Chan Partha Dutta Center for Pulmonary Vascular Biology and Medicine Pittsburgh HeartLungBloodand Vascular Medicine InstituteDivision of CardiologyDepartment of MedicineUniversity of Pittsburgh School of MedicineUniversity of Pittsburgh Medical CenterPittsburghPA15213USA Division of Pulmonary and Critical Care Medicine Zuckerberg San Francisco General Hospital and Trauma CenterUniversity of California San FranciscoBuilding 1002nd floor1001 Potrero AveSan FranciscoCAUSA Division of Pulmonary Allergy and Critical Care MedicineUniversity of PittsburghPittsburghPA15261USA Division of Hematopathology Pittsburgh HeartLungBloodand Vascular Medicine Institute University of Pittsburgh School of MedicineUniversity of Pittsburgh Medical CenterPittsburghPA15213USA Department of Medicine Anschutz Medical CampusBuilding RC29th floor12700 E 19th AveAuroraCO80045USA Department of Immunology University of Pittsburgh School of MedicinePittsburghPA15213USA

Myeloid cells,such as neutrophils,are produced in the bone marrow in high quantities and are important in the pathogenesis of vascular diseases such as pulmonary hypertension(PH).Although neutrophil recruitment into sites of inflammation has been well studied,the mechanisms of neutrophil egress from the bone marrow are not well *** computational flow cytometry,we observed increased neutrophils in the lungs of patients and mice with ***,we found elevated levels of IL-6 in the blood and lungs of patients and mice with *** observed that transgenic mice overexpressing Il-6 in the lungs displayed elevated neutrophil egress from the bone marrow and exaggerated neutrophil recruitment to the lungs,resulting in exacerbated pulmonary vascular remodeling,and dysfunctional ***,we found that IL-6-induced neutrophil egress from the bone marrow was dependent on interferon regulatory factor 4(IRF-4)-mediated CX3CR1 expression in ***,Cx3cr1 genetic deficiency in hematopoietic cells in Il-6-transgenic mice significantly reduced neutrophil egress from bone marrow and decreased neutrophil counts in the lungs,thus ameliorating pulmonary remodeling and *** summary,these findings define a novel mechanism of IL-6-induced neutrophil egress from the bone marrow and reveal a new therapeutic target to curtail neutrophil-mediated inflammation in pulmonary vascular disease.

关键词： neutrophil IL-6 pulmonary hypertension CX3CR1 inflammation

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Enantioseparation of Palonosetron Hydrochloride and Its Related Enantiomeric Impurities by Computer simulation and Validation

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American Journal of Analytical Chemistry 2011年第4期2卷 437-446页

作者： M.Vishnu Murthy C.Krishnaiah Kodithyala Jyothirmayi Katkam srinivas K.Mukkanti Ramesh kumar Gautam samanta Analytical Research and Development Integrated Product Development OperationsDr.Reddy’s Laboratories Ltd.BachupallyQutubullapurAndhra PradeshIndia Department of Chemistry Jawaharlal Nehru Technological UniversityHyderabadIndia

A rapid, simple and single stereo selective high-performance liquid chromatographic (HPLC) method was developed and validated for enantiomers of palonosetron hydrochloride (PALO) and its process related chiral impurities. A computer simulating software was used for the development of chiral method. The developed method was able to separate not only the enantiomers of palonosetron hydrochloride but also its process related chiral impurities within 12 min. The chromatographic separation was carried out by normal phase chromatography using a 3 μm column of cellulose based chiral stationary phase (Chiralcel-od 250mm × 4.6mm) with a mobile phase comprised of n-hexane: ethanol: methanol: heptafluoro butyric acid: diethyl amine (70:15:15:0.05:0.1, v/v) at a flow rate of 1.0 mL/min. The effects of additive concentration as well as nature of polar organic modifier, flow rate, and temperature on enantioselectivity were investigated. The limit of detection (Lod) and limit of quantification (LOQ) of the palonosetron isomers and its related chiral impurities were found to be in the range 0.06-0.10 μg/mL and 0.14 - 0.24 μg/mL respectively. The method showed excellent linearity (R2 > 0.998) over a range of 0.14 to 1.125 μg/mL. The percentage recovery of the isomers in bulk drug samples ranged from 87.0 to 116.0.

关键词： High Performance Liquid Chromatography Palonosetron Hydrochloride Chiral Impurities Drylab^(█) software Method Development Method Validation

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A Prospective, Multicentric, Post Marketing surveillance to Evaluate Efficacy & safety of Ranitidine HCl (150 & 300 mg IR/CR) in Indian Patients with Gastroesophageal Reflux Disease (PROGRADE)

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Open Journal of Gastroenterology 2023年第7期13卷 237-249页

作者： Akash shukla Anil kumar Awasthi Ramesh Rao Dawesh Prakash Yadav Nilesh Nolkha Rajesh Pendlimari sanjiv Dua shrish Bhatnagar Ravindra Mote Ashish Birla Jay savai Kapil Mehta shashank salunke Department of Gastroenterology Gastro Universe Mumbai India Department of Medicine Ajanta Research Centre Ajanta Hospital & IVF Centre Lucknow India Departmentof Nephrology Sanjeevani Kidney Care Mumbai India Department of Gastroenterology Institute of Medical Sciences Banaras Hindu University Varanasi India Department of Rheumatology Wockhardt Hospitals Mumbai India Department of Gastroenterology Rajalakshmi Hospital and Research Center Bangalore India Department of General surgery SaiKripa Hospital Mumbai India Department of Gastroenterology Sparsh Child Care and Gastro Centre Lucknow India Department of Clinical Research Mediclin Clinical Research Mumbai India Department of Medical Affairs JB Chemicals & Pharmaceuticals Ltd. Mumbai India

Purpose: Ranitidine hydrochloride (HCl) remains an important medication for treating acid-peptic ailments such as Gastroesophageal reflux disease (GERD). The main objective of this Post Marketing surveillance (PMs) clinical study was to test the efficacy and safety of Ranitidine HCl in Indian patients suffering from GERD. Patients and Methods: Data of 2446 patients (1307 males;1121 females) from 21 centers across India were analyzed. Patients received either of the three treatments: Ranitidine HCl 150 mg twice a day (BID) (ARM-A), Ranitidine HCl 300 mg once daily (od) or BID (ARM-B), and Ranitidine HCl 300 mg od (ARM-C). Gastroesophageal Reflux Disease symptom Assessment scale (GsAs) score and Heartburn severity score were used to assess the drug’s efficacy. The adverse events reported by patients or investigators were analyzed to assess the safety profile of Ranitidine. Results: Of the 2446 subjects screened, 2428 were enrolled. There was a significant reduction in GsAs scores from baseline to the end of the study visit in all three ARMs. The GsAs scores reduced from 2.02 to 0.23 in ARM-A, 2.01 to 0.24 in ARM-B, and 2.07 to 0.26 in ARM-C patients. In ARM A, 72.82% had 24 hours heartburn-free days, and 66.89% had 7 consecutive heartburn-free days, which was more significant than the other two ARMs. 128 (5.27%) patients reported ADRs due to Ranitidine HCl at different doses. The most frequently reported ADR was constipation (17.18%), followed by oliguria (14.06%), cold (13.28%), and dysuria (12.5%). Of 128 ADRs, 113 (88.28%) were mild, and only 11 (8.59%) ADRs were related to the study drug. No severe ADRs were reported during the study. Conclusion: Ranitidine HCl 150/300 mg tablet was found to be an effective and safe H2-receptor antagonist for treating GERD in Indian Patients.

关键词： Ranitidine Hydrochloride GERD Heartburn H2-Receptor Antagonists

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Nature:科学家鉴别出调节机体睡眠的两个关键基因

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现代生物医学进展 2017年第6期17卷 I0002-I0002页

作者： Hiromasa Funato, Chika Miyoshi, Tomoyuki Fujiyama, Takeshi Kanda, Makito sato, Zhiqiang Wang, Jing Ma, Aya Ikkyu, Miyo Kakizaki, Noriko Hotta-Hirashima, satomi Kanno, Haruna Komiya, Fuyuki Asano, Takato Honda, staci J. Kim, Kanako Harano, Hiroki Muramoto, Toshiya Yonezawa, shinichi Miyazaki, Linzi Connor, Yu Hayashi, Qinghua Liu, Joseph s. Takahashi Masashi Yanagisawa Hiromasa Funato Makito sato Masashi Yanagisawa shin Nakane, Jun Tomita Kazuhiko Kume seiya Mizuno, Fumihiro sugiyama satoru Takahashi Vivek kumar Joseph s. Takahashi Vivek kumar Ikuo Miura, Tomohiro suzuki shigeharu Wakana Atsushi Watanabe Manabu Abe Kenji sakimura Yu Hayashi Qinghua Liu Joseph s. Takahashi Masashi Yanagisawa Masashi Yanagisawa International Institute for Integrative sleep Medicine (WPI-IIIs) University of Tsukuba Tsukuba Ibaraki 305-8575 Japan Department of Anatomy Faculty of Medicine Toho University Ota-ku Tokyo 143-8540 Japan Department of Molecular Genetics University of Texas Southwestern Medical Center Dallas Texas 75390 USA Department of Neuropharmacology Graduate School of Pharmaceutical Sciences Nagoya City University Nagoya Aichi 467-8603 Japan Laboratory Animal Resource Center University of Tsukuba Tsukuba Ibaraki 305-8575 Japan Department of Neuroscience University of Texas Southwestern Medical Center Dallas Texas 75390 USA The Jackson Laboratory Bar Harbor Maine 04609 USA Technology and Development Team for Mouse Phenotype Analysis RIKEN Bioresource Center Tsukuba Ibaraki 305-0074 Japan Laboratory of Research Advancement National Center for Geriatrics and Gerontology Obu Aichi 474-8511 Japan Department of Cellular Neurobiology Brain Research Institute Niigata University Niigata 951-8585 Japan PREsTO Japan Science and Technology Agency Kawaguchi Saitama 332-0012 Japan Department of Biochemistry University of Texas Southwestern Medical Center Dallas Texas 75390 USA Howard Hughes Medical Institute University of Texas Southwestern Medical Center Dallas Texas 75390 USA Life science Center Tsukuba Advanced Research Alliance University of Tsukuba Tsukuba Ibaraki 305-8575 Japan

近日，刊登在国际杂志Nature上的一项研究报告中，来自西南医学中心等机构的研究人员通过研究鉴别出了两个关键的核心基因，这两个基因能够帮助调节机体深度睡眠和做梦的水平，相关研究或为阐明相关的基因控制睡眠的网络提供新的线索。... 详细信息

近日，刊登在国际杂志Nature上的一项研究报告中，来自西南医学中心等机构的研究人员通过研究鉴别出了两个关键的核心基因，这两个基因能够帮助调节机体深度睡眠和做梦的水平，相关研究或为阐明相关的基因控制睡眠的网络提供新的线索。此前研究者通过对小鼠进行研究发现了能够控制机体快速眼动睡眠的基因，同时该基因还能够诱导深度睡眠。而第二个基因能够控制机体对快速眼动睡眠的需求量，相关研究为科学家们提供了一个关键的切入点来帮助解释睡眠工作的机制，同时研究者还鉴别出了治疗睡眠障碍的潜在靶点。

关键词：睡眠障碍核心基因科学家机体鉴别基因控制研究人员需求量

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