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Expression of p53,Bax and Bcl-2 proteins in hepatocytes in non-alcoholic fatty liver disease

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World Journal of Gastroenterology 2006年第38期12卷 6198-6202页

作者： anatol Panasiuk Janusz Dzieciol Bozena Panasiuk Danuta Prokopowicz Department of Infectious Diseases Medical University of Biaystok Poland Department of Human Anatomy Medical University of Bialystok Poland

AIM: To analyze the protein expression essential for apoptosis in liver steatosis. METHODS: The expression of proapoptotic proteins p53, Bax, and antiapoptotic Bcl-2 in hepatocytes with steatosis (SH) and without steatosis (NSH) was evaluated in 84 patients at various stages of non-alcoholic fatty liver disease (NAFLD). RESULTS: Immunohistochemical staining of liver tissue showed the activation of p53 protein in SH and NSH with increased liver steatosis, diminished Bcl-2 and slightly decreased Bax protein. Positive correlation was found between the stage of liver steatosis with p53 expression in SH (r = 0.54, P < 0.01) and NSH (r = 0.49, P < 0.01). The antiapoptotic protein Bcl-2 was diminished together with the advancement of liver steatosis, especially in non-steatosed hepatocytes (r =0.43, P < 001). CONCLUSION: Apoptosis is one of the most important mechanisms leading to hepatocyte elimination in NAFLD. The intensification of inflammation in NAFLD induces proapoptotic protein p53 with the inhibition of antiapoptotic Bcl-2.

关键词： Apoptosis Non-alcoholic liver disease p53 Bcl-2 Bax Immunohistochemistry

Predictors of new-onset atrial fibrillation in elderly patients with coronary artery disease after coronary artery bypass graft

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Journal of Geriatric Cardiology 2016年第5期13卷 444-449页

作者： Rubanenko O anatol'evna Fatenkov O Veniaminovic Khokhlunov S Mikhaylovich Department of Faculty Therapy Samara State Medical Universtty Samara Russia Samara Regional Cardiology Centre Samara Russia

Objective To identify the factors associated with the development of postoperative atrial fibrillation （POAF） after coronary artery bypass graft （CABG） in elderly patients with coronary artery disease （CAD）. Methods A total of 81 patients with CAD who underwent CABG were enrolled in the study. Patients were divided into two groups： Group 1, without postoperative atrial fibrillation （59 patients, 74.6% men, mean age 65.8 ~ 4.0 years）; Group 2, with early new-onset atrial fibrillation after CABG （22 patients, 90.9% men, mean age 67.7 ＋ 5.4 years）. Interleukin （IL）-6, IL-8, IL-10, C-reactive protein （CRP）, fibrinogen, superoxide dismutase （SOD）, N-terminal pro-B-type natriuretic peptide （NT-proBNP）, and troponin I were studied. Results During the observation period, atrial fibrillation occurred in 27.2% cases, an average of 4.9 ±3.8 days after surgery. In group 2, the left atrium （LA） dimension was larger than in group 1 （43.9 ± 3.4 mm vs. 37.6 ±3.9 rnm, P 〈 0.001）. Patients with POAF had significantly higher IL-6 （72.7 ±60.8 pg/mL vs. 38.0 ± 34.6 pg/mL, P = 0.04）, IL-8 （11.9 ± 6.0 pg/mL vs. 7.7± 5.4 pg/mL, P = 0.01） and SOD （2462.0 ± 2029.3 units/g vs. 1515.0 ± 1292.9 units/g, P = 0.04） compared with group without POAF. The multivariate analysis showed that the odds ratio （OR） for POAF development in patients with left atrium more than 39 mm was 2.1 [95% confidence interval （CI）： 1.2-3.8, P = 0.0004], IL-6 levels more than 65.18 pg/mL-1.4 （95% Ch 1.1-2.7, P = 0.009）, IL-8 levels more than 9.67 pg/mL-1.2 （95% CI： 1.1-3.7, P ： 0.009）, SOD more than 2948 units/g-1.1 （95% Ch 1.01-2.9, P = 0.04）. Conclusions In our study, the independent predictors of postoperative atrial fibrillation after CABG in elderly patients were left atrium dimension and the increased postoperative concentration of IL-6, IL-8 and superoxide dismutase.

关键词： Antioxidant Atrial fibrillation Coronary artery bypass graft Interleukins Troponin

Blood platelet and monocyte activations and relation to stages of liver cirrhosis

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World Journal of Gastroenterology 2005年第18期11卷 2754-2758页

作者： anatol Panasiuk Janusz Zak Edwina Kasprzycka Katarzyna Janicka Danuta Prokopowicz Department of Infectious Diseases Medical University of Bialystok Poland Department of Pediatrics Diagnostics Medical University of Bialystok Poland

AIM: Blood platelets (pIt) and monocytes are the cells that play a crucial role in the pathogenesis of liver damage and liver cirrhosis (LC). In this paper, the analysis of mutual relationship between platelets and monocytes activation in LC was conducted. METHODS: Immunofluorescent flow cytometry was used to measure the percentage of activated platelet populations (CD62P, CD63), the percentage of plt-monocyte aggregates (pma) (CD41/CD45), and activated monocytes (CD11b, CD14, CD16) in the blood of 20 volunteers and 40 patients with LC. Platelet activation markers: sP-selectin, platelet factor 4 (PF4), beta-thromboglobulin (PTG) and monocyte chemotactic peptide-1 (MCP-1) were measured and compared in different stages of LC. RESULTS: Platelet activation with the increase in both βTG serum concentration and elevation of pIt population (CD62P and CD63 as well as MIF CD62P and CD63) is elevated as LC develops and thrombocytopenia rises. There is a positive correlation between medial intensity of fluorescence (MIF) CD62P and MIF CD63 in LC. We did not show any relationship between monocyte activation and pma level. SP-selectin concentration correlates positively with pIt count and pma, and negatively with stage of pIt activation and MIF CD62P and MIF CD63. There was no correlation between MCP-1 concentration and pIt, monocyte activation as well as pma level in LC. CD16 monocytes and MIF CD16 populations are significantly higher in the end stage of LC. A positive correlation occurs between the value of CDllb monocyte population and MIF CD14 and MIF CD16 on monocytes in LC. CONCLUSION: Platelet and monocyte activation plays an important role in LC. Platelet activation stage does not influence monocyte activation and production of pIt aggregates with monocytes in LC. With LC development, thrombocytopenia may be the result of pIt consumption in platelet-monocyte aggregates.

关键词： Blood platelet Monocyte Liver cirrhosis Flow cytometry

Soluble adhesion molecules ICAM-1,VCAM-1,P-selectin in children with Helicobacter pylori infection

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World Journal of Gastroenterology 2005年第43期11卷 6745-6750页

作者： Elzbieta Maciorkowska Maciej Kaczmarski anatol Panasiuk Katarzyna Kondej-Muszynska Andrzej Kemonai Department of Pediatric Nursing 3rd Department of Children's Diseases Department of Infectious Diseases Department of Pathomorphology

AIM: To assess the sICAM-1, sVCAM-1, and sP-selectin levels in children withHelicobacter pylori(H pylori)infection and to evaluate their significance for the morphological changes found in gastric ***: The study included 106 children: 59children (55.7%) with chronic gastritis and positive IgG against H pylori, 29 children (27.3%) after previous H pylori infection without the bacterium colonization but with positive IgG against H pylori, and 18 children (17%) with functional disorders of the gastrointestinal system but with normal IgG against H pylori. Endoscopic and histopathological evaluation of gastric mucosa was performed based on the Sydney System *** evaluation of sP-selectin, sIC AM-1, sVCAM-1 levels in the sera of children was carried out using ELISA ***: The assessment of gastritis activity degrees indicated statistically significant values in the antrum and corpus (P＜0.001) of children examined. Serum sVCAM-1 levels were higher in group with gastritis due to H pylori infection than in group without infection and differed statistically (P＜0.05). Serum sVCAM-1 levels proved to be the highest among other adhesive molecules in infected children and decreased after eradication of H pylori. Serum sICAM-1 levels were similar in all examined groups. Serum sP-selectin levels were similar in children with and without H pylori ***: Assessment of adhesive molecules (sPselectin, sICAM-1, sVCAM-1) in the sera of children with active H pylori infection can show the participation of sVCAM-1 in the pathogenesis of gastric mucosal inflammation, sP-selectin and sICAM-1 concentrations in the sera of children with H pylori infection after eradication cannot reveal any significant differences as compared to healthy children.

关键词： sVCAM-1 sICAM-1 sP-selectin Helicobacter pylori

Expression of β2-integrin on leukocytes in liver cirrhosis

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World Journal of Gastroenterology 2006年第38期12卷 6193-6197页

作者： anatol Panasiuk Janusz ■ak Elzbieta Maciorkowska Bozena Panasiuk Danuta Prokopowicz Department of Infectious Diseases Medical University of Bialystok Poland Department of Pediatrics Diagnostics Medical University of Bialystok Poland Department of Pediatric Nursing Medical University of Bialystok Poland

AIM： To analyze β2-integrin expression on blood leukocytes in liver drrhosis.METHODS： In 40 patients with liver cirrhosis and 20 healthy individuals, the evaluation of expression of CD11a （LFA-1α）, CD11b （Mac-1α）, CD11c （αX） and CD49d （VLA-4α） on peripheral blood leukocytes was performed using flow cytometry. The analysis was carried out in groups of patients divided into B and C according to Child-Pugh＇s classification. RESULTS： An increased CDlla, CD11b, CD11c and CD49d integrin expression was observed on peripheral blood leukocytes in liver cirrhosis. The integrin levels were elevated as the advancement of liver failure progressed. The highest expression of integrins occurred predominantly on monocytes. A slight expression of VLA-4 was found on lymphocytes and granulocytes and it increased together with liver failure. A positive correlation was noted between median intensity of fluorescence （MIF） expression on polymorphonuclear cells of CD11a and CD11c and CD49d （r = 0.42, P 〈 0.01; r = 053, P 〈 0.01, respectively） in liver cirrhosis stage C. However, no correlation was observed between integrin expression on leukocytes. The concentrations of sICAM-1, sVCAM-1, and TNFα, were significantly elevated in liver cirrhosis. CONCLUSION： β2-integrin expression on leukocytes increases in liver cirrhosis decornpensated as the stage of liver failure increases, which is a result of permanent activation of leukooytes circulating through the inflamed liver environment.β2-integrin expression on circulating leukocytes can intensify liver cirrhosis,

关键词： β2-integrin Liver cirrhosis Flow cytometry Leukocytes

Miniature,highly sensitive MOSCAP ring modulators in co-optimized electronic-photonic CMOS

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Photonics Research 2022年第1期10卷 I0001-I0007页

作者： HAYK GEVORGYAN anatol KHILO MARK T.WADE VLADIMIR M.STOJANOVIC MILOS A.POPOVIC Department of Electrical and Computer Engineering Boston UniversityBostonMassachusetts 02215USA Ayar Labs Inc. EmeryvilleCalifornia 94608USA Department of Electrical Engineering and Computer Sciences University of California BerkeleyBerkeleyCalifornia 94709USA

Convergence of high-performance silicon photonics and electronics,monolithically integrated in state-of-the-art CMOS platforms,is the holy grail for enabling the ultimate efficiencies,performance,and scaling of electronicphotonic *** requires the emergence of platforms that combine state-of-the-art RF transistors with optimized silicon photonics,and a generation of photonic device technology with ultralow energies,increased operating spectrum,and the elimination of power-hungry thermal *** this paper,in a co-optimized monolithic electronics-photonics platform(GlobalFoundries 45 CLO),we turn the metal-oxide-semiconductor(MOS) field-effect transistor’s basic structure into a novel,highly efficient MOS capacitor ring *** has the smallest ring cavity(1.5 μm radius),largest corresponding spur-free free spectral range(FSR=8.5 THz),and record 30 GHz/V shift efficiency in the O-band among silicon modulators demonstrated to *** 1 V_(pp) RF drive,we show an open optical eye while electro-optically tuning the modulator to track over 400 pm(69 GHz) change in the laser wavelength(using 2.5 V_(DC) range).A 90 GHz maximum electro-optic resonance shift is demonstrated with under 40 nW of power,providing a strong nonthermal tuning mechanism in a CMOS photonics *** modulator has a separately optimized body layer but shares the gate device layer and the gate oxide with 45 nm transistors,while meeting all CMOS manufacturability design *** type of convergent evolution of electronics and photonics may be the future of platforms for high-performance systemson-chip.

关键词： tuning modulator electronic

Immunological and functional features of decellularized xenogeneic heart valves after transplantation into GGTA1-KO pigs

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Regenerative Biomaterials 2021年第5期8卷 25-36页

作者： Robert Ramm Tobias Goecke Peter Kohler Igor Tudorache Serghei Cebotari anatol Ciubotaru Samir Sarikouch Klaus Hoffler Friederike Bothe Bjorn Petersen Axel Haverich Heiner Niemann Andres Hilfiker Leibniz Research Laboratories for Biotechnology and Artificial Organs(LEBAO) Hannover Medical SchoolCarl-Neuberg Str.1Hannover 30625Germany Department of Cardiac- Thoracic-Transplantation and Vascular SurgeryHannover Medical SchoolCarl-Neuberg Str.1Hannover 30625Germany Institute of Farm Animal Genetics Friedrich-Loeffler-InstitutHoeltystr.1031535 Mariensee/Neustadt am RuebenbergeGermany Medimplant GmbH Bischofsholer Damm 15Hannover 30173Germany

Decellularization of xenogeneic heart valves might lead to excellent regenerative implants,from which many patients could ***,this material carries various xenogeneic epitopes and thus bears a considerable inherent immunological ***,we investigated the regenerative and immunogenic potential of xenogeneic decellularized heart valve implants using pigs deficient for the galactosyltransferase gene(GGTA1-KO)as novel large animal *** aortic and pulmonary heart valves obtained from sheep,wild-type pigs or GGTA1-KO pigs were implanted into GGTA1-KO pigs for 3,or 6 months,*** were analyzed histologically,immunhistologically(CD3,CD21 and CD172a)and anti-aGal antibody serum titers were determined by *** sheep derived implants exhibited a strong immune reaction upon implantation into GGTA1-KO pigs,characterized by massive inflammatory cells infiltrates,presence of foreign body giant cells,a dramatic increase of anti-aGal antibody titers and ultimately destruction of the graft,whereas wild-type porcine grafts induced only a mild reaction in GGTA1-KO *** implants,wild-type/wild-type and GGTA1-KO/GGTA1-KO valves did not induce a measurable immune ***,GGTA1-KO pigs developed a‘human-like’immune response toward decellularized xenogeneic implants showing that immunogenicity of xenogeneic implants is not sufficiently reduced by decellularization,which detracts from their regenerative potential.

关键词： aGal-KO pig decellularization heart valves xenoantibodies large animal model

Hetero-integrated high-peak-optical-power laser source (940 nm) for time-of-flight sensors

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Chinese Optics Letters 2024年第7期22卷 81-85页

作者： Sergey Olegovich Slipchenko Aleksandr Aleksandrovich Podoskin Ilia Vasil'evich Shushkanov Marina Gennad'evna Rastegaeva Artem Eduardovich Rizaev Matvey Igorevich Kondratov Artem Evgen'evich Grishin Nikita Aleksandrovich Pikhtin Timur anatol'evich Bagaev Maxim anatol'evich Ladugin Aleksandr anatol'evich Marmalyuk Vladimir Aleksandrovich Simakov Ioffe Institut Russian Academy of SciencesSt.Petersburg 194021Russia Open Joint-Stock Company M.F.Stel’makh Polyus Research Institute Moscow 117342Russia RUDN University Moscow 117198Russia

The generation of high-power laser pulses using a compact hetero-integrated assembly based on a semiconductor laser with a dual-element composite 2μm×100μm aperture and a compact heterothyristor switch is *** achieved peak optical power was 33 W with a pulse duration of 3 ns at a thyristor operating voltage of 55 *** leading edge of the laser pulse turn-on was 50 ps to a power level of 24.7 W,and the turn-on delay between the elements of the composite aperture was 160 ps.

关键词： hetero-integrated assembly semiconductor lasers thyristor switches LIDAR gain-switching mode laser heterostructure

纳米流体驱提高原油采收率的三维孔隙尺度模拟

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石油勘探与开发 2021年第4期48卷 825-834页

作者： MINAKOV Andrey Viktorovich GUZEI Dmitriy Viktorovich PRYAZHNIKOV Мaxim Ivanovich FILIMONOV Sergey anatol'yevich VORONENKOVA Yulia Olegovna 西伯利亚联邦大学俄罗斯克拉斯诺亚尔斯克660041 库塔捷拉泽热物理研究所俄罗斯科学院西伯利亚分院俄罗斯新西伯利亚630090

基于不同渗透率岩心的三维微观模型,采用流体体积法对纳米流体驱油进行数值模拟;利用实验测得的含SiO_(2)纳米颗粒的悬浮液界面张力、接触角和黏度,针对质量分数为0~1%、具有不同粒径的SiO_(2)纳米颗粒的水基悬浮液,研究了纳米颗粒质量... 详细信息

基于不同渗透率岩心的三维微观模型,采用流体体积法对纳米流体驱油进行数值模拟;利用实验测得的含SiO_(2)纳米颗粒的悬浮液界面张力、接触角和黏度,针对质量分数为0~1%、具有不同粒径的SiO_(2)纳米颗粒的水基悬浮液,研究了纳米颗粒质量分数和粒径、驱替液流速、原油黏度和岩心渗透率对纳米流体驱油效率的影响。研究表明:采收率随着颗粒质量分数的增加而增加,纳米颗粒质量分数增加到0.5%时,与水驱相比采收率可提高约19%;纳米流体驱油采收率随着纳米颗粒粒径的减小而增加;在毛管数接近临界值的注入模式下使用纳米流体驱油对高黏低渗储集层提高采收率最为有效,且提高采收率幅度随着驱替速度的增加而减小;原油黏度越大,岩石渗透率越小,纳米流体驱油提高采收率的效果越明显。图16表2参37。

关键词：纳米流体驱油提高采收率孔隙尺度模拟流体体积法