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Delivery of therapeutics to posterior eye segment:cell-encapsulating systems

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Neural Regeneration Research 2017年第4期12卷 576-577页

作者： francisca s.y.wong ken k.tsang amy c.y.lo department of ophthalmology li ka shing faculty of medicinethe university of hong kongHong Kong Special Administrative RegionChina research center of heart brainhormone and healthy agingli ka shing faculty of medicinethe university of hong kongHong Kong Special Administrative RegionChina

Drug delivery to the posterior segment of the eye, especially the retina, faces the unique challenges of various anatomical and physiological barriers. Invasive intravitreal injection is currently the mainstream clinical approach to posterior eye diseases such as age-related macular degeneration, diabetic retinopathy, uveitis, and cytomegalovirus retinitis. Repeated injections could be complicated by endophthalmitis, intra- ocular pressure elevation, retinal vascular occlusion, and ret- inal detachment, let alone the psychological and economical burdens suffered by patients （Schwartz et al., 2014）. In view of this, several extended release drug delivery systems have been devised and the idea of encapsulated-cell technology （EcT） has gained popularity in recent decades since its first proposal in the 1960s.

关键词： cell-encapsulating systems Delivery of therapeutics to posterior eye segment

Prostanoid synthases and receptors in aorta of spontaneously hypertensive rat

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Prostanoid synthases and receptors in aorta of spontaneously...

Twisted fiber microfluidics:a cutting-edge approach to 3D spiral devices

11th International Vascular Neuroeffector Mechanisms and cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006

作者： Eva H.c.Tang George P.M.Leung amy c.y.lo Sookja K.chung Ricky y.K.Man Paul M.Vanhoutte Dept of Pharmacology University of HK Hong Kong Dept of Pharmacology University of HK Hong Kong Dept of Anatomy University of HK Hong Kong Dept of Anatomy University of HK Hong Kong Dept of Pharmacology University of HK Hong Kong Dept of Pharmacology University of HK Hong Kong

Expression pattern of prostanoid synthases on freshly isolated endpthelial cells and its respective receptors on smooth muscle cells of the thoracic aorta of eight-month old Wistar Kyoto rat (WKy) and spontaneously hypertensive rat (SHR) are assessed. Total RNA was isolated, converted into cDNA and used for quantitative real-time polymerase chain reaction (PcR) using SyBR green fluorescent dye to quantify the accumulation of double-stranded PcR

关键词： Prostanoid synthases and receptors in aorta of spontaneously hypertensive rat

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Microsystems & Nanoengineering 2024年第1期10卷 193-202页

作者： Shunsuke Kato Daniel W.carlson amy Q.Shen yuanyuan Guo Department of Electrical Information and Physics EngineeringSchool of EngineeringTohoku UniversityAoba-kuSendai 980-8579 MiyagiJapan Micro/Bio/Nanofluidics Unit Okinawa Institute of Science and TechnologyOnnaKunigami-gun 904-0495 OkinawaJapan Frontier Research Institute for Interdisciplinary Sciences(FRIS) Tohoku UniversityAoba-kuSendai 980-0845 MiyagiJapan Graduate School of Biomedical Engineering Tohoku UniversityAoba-kuSendai 980-8579 MiyagiJapan Department of Physiology Graduate School of MedicineTohoku UniversityAoba-kuSendai 980-8575 MiyagiJapan

The development of 3D spiral microfluidics has opened new avenues for leveraging inertial focusing to analyze small fluid volumes,thereby advancing research across chemical,physical,and biological *** traditional straight microchannels rely solely on inertial lift forces,the novel spiral geometry generates Dean drag forces,eliminating the necessity for external fields in fluid ***,fabricating 3D spiral microfluidics remains a labor-intensive and costly endeavor,hindering its widespread ***,conventional lithographic methods primarily yield 2D planar devices,thereby limiting the selection of materials and geometrical *** address these challenges,this work introduces a streamlined fabrication method for 3D spiral microfluidic devices,employing rotational force within a miniaturized thermal drawing process,termed as *** innovation allows for rapid prototyping of twisted fiber-based microfluidics featuring versatility in material selection and heightened geometric *** validate the performance of these devices,we combined computational modeling with microtomographic particle image velocimetry(uTPiM)to comprehensively characterize the 3D flow *** results corroborate the presence of a steady secondary flow,underscoring the effectiveness of our *** 3D spiral microfluidics platform paves the way for exploring intricate microflow dynamics,with promising applications in areas such as drug delivery,diagnostics,and lab-on-a-chip systems.

关键词： thereby straight twisted

The HIF-1α/PloD2 axis integrates extracellular matrix organization and cell metabolism leading to aberrant musculoskeletal repair

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Bone Research 2024年第2期12卷 289-301页

作者： Heeseog Kang amy L.Strong yuxiao Sun Lei Guo conan Juan Alec c.Bancroft Ji Hae choi chase A.Pagani Aysel A.Fernandes Michael Woodard Juhoon Lee Sowmya Ramesh Aaron W.James David Hudson Kevin N.Dalby Lin Xu Robert J.Tower Benjamin Levi center for Organogenesis Regeneration and TraumaDepartment of SurgeryUniversity of Texas SouthwesternDallasTX 75390USA Section of Plastic Surgery Department of SurgeryUniversity of MichiganAnn ArborMI 48109USA Quantitative Biomedical Research center Peter O’Donnell Jr.School of Public HealthUniversity of Texas SouthwesternDallasTX 75390USA Department of Orthopedics and Sports Medicine University of WashingtonSeattleWA 98195USA Division of chemical Biology and Medicinal chemistry College of PharmacyUniversity of Texas at AustinAustinTX 78712USA Department of Pathology Johns Hopkins UniversityBaltimoreMD 21218USA

While hypoxic signaling has been shown to play a role in many cellular processes,its role in metabolism-linked extracellular matrix(EcM)organization and downstream processes of cell fate after musculoskeletal injury remains to be ***(HO)is a debilitating condition where abnormal bone formation occurs within extra-skeletal *** andhypoxia-inducible factor 1α(HIF-1α)activation have been shown to promote ***,the underlying molecular mechanisms bywhich the HIF-1αpathway in mesenchymal progenitor cells(MPcs)contributes to pathologic bone formation remain to ***,we used a proven mouse injury-induced HO model to investigate the role of HIF-1αon aberrant cell ***-cell RNA sequencing(scRNA-seq)and spatial transcriptomics analyses of the HO site,we found that collagen EcM organizationis the most highly up-regulated biological process in *** mesenchymal cell-specific deletion of Hif1α(Hoxa11-creER^(T2);Hif1a^(fl/fl))significantly mitigated HO in ***-seq analysis of these Hoxa11-creER^(T2);Hif1a^(fl/fl)mice identified the PloD2/loXpathway for collagen cross-linking as downstream of the HIF-1αregulation of ***,our scRNA-seq data and mechanisticstudies further uncovered that glucose metabolism in MPcs is most highly impacted by HIF-1α*** a translational aspect,a pan-loX inhibitor significantly decreased HO.A newly screened compound revealed that the inhibition of PloD2 activity in MPcssignificantly decreased osteogenic differentiation and glycolytic *** suggests that the HIF-1α/PloD2/loX axis linked tometabolism regulates HO-forming MPc *** results suggest that the HIF-1α/PloD2/loX pathway represents a promisingstrategy to mitigate HO formation.

关键词： metabolism skeletal aberrant

Lycium barbarum polysaccharides and ferroptosis:jumping into the era of novel regulated cell death

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Neural Regeneration Research 2022年第7期17卷 1473-1474页

作者： amy cheuk yin lo Ming yang Department of Ophthalmology Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong Special Administrative RegionChina

Lycium barbarum polysaccharides(LBP)are the key bioactive components of Lycium barbarum(also named Gouqizi or Goji berry or wolfberry),a widely used Traditional chinese herb for more than 2000 *** to balance"yin"and"y... 详细信息

关键词： benefits consumed balance

Smart phone apps every ophthalmologist should know about

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International Journal of Ophthalmology(English edition) 2020年第8期13卷 1329-1333页

作者： Ming yang amy cheuk yin lo Wai ching Lam Department of Ophthalmology Li Ka Shing Faculty of Medicinethe University of Hong KongHong Kong Special Administrative Region 999077China

We are living in a mobile world,where the number of medical apps is surging in recent *** 320000 healthcare apps are now available as shown in the latest *** major function and potential of these apps vary,from assisting the learning and practice of clinicians as well as achieving better outcomes of patients to preventing eye diseases through the education of healthy ***,based on the various features,ophthalmology apps nowadays contain a wide range of subjects and focus on dif ferent stakeholder s in the ophthalmic practice,including but not limited to doctors,patients,and ***,we review special and advanced apps that ophthalmologists will find useful in their clinical practice.

关键词： applications healthcare clinic ophthalmology ophthalmologist

Ferroptosis and glaucoma: implications in retinal ganglion cell damage and optic nerve survival

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Neural Regeneration Research 2023年第3期18卷 545-546页

作者： Ming yang Kwok-Fai So Wai-ching Lam amy cheuk yin lo Department of Ophthalmology School of Clinical MedicineLi Ka Shing Faculty of MedicineThe University of Hong KongHong Kong Special Administrative RegionChina State Key Laboratory of Brain and cognitive Sciences The University of Hong KongHong Kong Special Administrative RegionChina GHM Institute of cNS Regeneration Jinan UniversityGuangzhouGuangdong ProvinceChina

Glaucoma and visual pathway degeneration:Glaucoma is the leading cause of irreversible blindness worldwide,which leads to a progressive loss of *** can be classified into two types:primary open-angle glaucoma and primary closed-angle *** open-angle glaucoma can be caused by the blockage of the trabecular meshwork,and this results in elevation of the intraocular pressure(IOP),leading to retinal ganglion cell(RGc)***,many glaucoma patients have normal IOP;this is known as normal-tension ***,excitotoxic damage and oxidative stress can also lead to RGc damage in normal-tension glaucoma(Almasieh et al.,2012).Glaucomatous genes such as TIGR,OPTN,and cyP1B1 have been suggested to contribute to the pathogenesis of ***,some glaucomatous patients may remain asymptomatic in the early,moderate,and late *** type of glaucoma is primary closed-angle *** this clinical condition,a relative pupillary block is contributed by the iris obstructing aqueous *** patients may suffer from corneal swelling,headache,nausea,and blurred vision during the acute phase.

关键词： glaucoma damage degeneration

Induction of significant intraocular pressure diurnal fluctuation in rats using a modified technique of microbead occlusion

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International Journal of Ophthalmology(English edition) 2018年第7期11卷 1114-1119页

作者： Lin Fu Jimmy Shiu Ming Lai amy cheuk yin lo Kendrick co Shih Affiliated Eye Hospital School of Ophthalmology and Optometry Wenzhou Medical University Department of Ophthalmology Li Ka Shing Faculty of Medicine University of Hong Kong

AIM： To investigate the effect of microbead iridocorneal angle occlusion on intraocular pressure（IOP） diurnal fluctuation in rat eyes. METHODS： Male Dark Agouti（DA） rats, 8-10 week old, were each given a single intracameral injection of microbeads, followed by injection of dispersive viscoelastic solution. The right eye served as the experimental eye, while the left eye served as the control. IOP was measured twice daily postoperatively for 3 wk and compared between groups. At the end of 3 wk, the rats were sacrificed and the eyes were harvested for histological analysis and retinal ganglion cell（RGc） counting. RESULTS： After microbead injection, experimental eyes had significantly higher dark time IOP than controls from the second week to the third week [2 nd week： 22.92±1.631 mm Hg（n=5） vs 17.35±0.751 mm Hg（n=5）; 3 rd week： 23.59±1.494 mm Hg vs 17.73±0.592 mm Hg（n=5）], while light time IOP was comparable between groups. The fluctuation levels of IOP in the experimental eyes were 7.21±0.398 mm Hg（n=5）, 9.50±1.017 mm Hg（n=5） and 10.66±0.894 mm Hg（n=5） from the first week to the third week after injection. comparatively, they were significantly lower in the control eyes, which were 4.69±0.323 mm Hg（n=5）, 2.84±1.122 mm Hg（n=5） and 4.98±0.603 mm Hg（n=5） respectively. However, at the end of 3 wk, the larger fluctuations in IOP in the experimental eyes was not associated with a significant loss of RGcs. cONcLUSION： Microbead occlusion exacerbates diurnal IOP fluctuation in rats. This reported model may serve as a method of investigating the pathological effects of IOP fluctuation. A longer observation period, or repeated injections, may be needed to observe a significant change in RGc density.

关键词： microbead occlusion diurnal intraocular pressure fluctuation glaucoma rat

comparison of electroretinographic responses between two different age groups of adult Dark Agouti rats

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International Journal of Ophthalmology(English edition) 2015年第5期8卷 898-903页

作者： Lin Fu amy cheuk yin lo Jimmy Shiu Ming Lai Kendrick co Shih Department of Ophthalmology LKS Faculty of MedicineUniversity of Hong Kong

AIM: To describe and compare the differences in electroretinographic responses between two different age groups of adult Dark Agouti (DA) rats and to better understand the effect of age on retinal histology and function. METHODS: The electroretinographic responses of two different age groups of adult DA rats were compared. Animals were divided into younger adult DA rats 10-12wk (n =8) and older adult DA rats 17-19wk (n=8). Full field electroretinography (ERG) was recorded simultaneously from both eyes after dark adaption and light adaption and parameters including the positive scotopic threshold response (pSTR), negative scotopic threshold response (nSTR), scotopic a -wave, b -wave, photopic a -wave, b -wave and photopic negative response (PhNR) were compared between groups. RESULTS: The older adult rats displayed lower stimulation thresholds of the STRs (pSTR and nSTR) and higher amplitudes of pSTR, scotopic a -wave and b wave, photopic b -wave and PhNR amplitudes, with shorter implicit times. Photopic a-wave amplitudes were however higher in the younger adult rats. cONcLUSION: In summary, for the rod system, photoreceptor, bipolar cell and RGc activity was enhanced in the older adult rats. For the cone system, RGc and bipolar cell activity was enhanced, while photoreceptor activity was depressed in the older adult rats. Such age -related selective modification of retinal cell function needs to be considered when conducting ophthalmic research in adult rats.

关键词： electroretinographic responses Dark Agouti rats age