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Mice lacking perforin have improved regeneration of the injured femoral nerve

Neural Regeneration Research 2022年第8期17卷 1802-1808页

作者： Igor Jakovčevski Monika von düring david Lutz Maja Vulović Mohammad Hamad Gebhard Reiss Eckart Förster Melitta Schachner Institut für Anatomie und Klinische Morphologie Universität Witten/HerdeckeWittenGermany department of Neuroanatomy and Molecular Brain Research Ruhr University BochumBochumGermany department of Anatomy Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia Keck Center for Collaborative Neuroscience and department of Cell Biology and Neuroscience Rutgers UniversityPiscatawayNJUSA

The role that the immune system plays after injury of the peripheral nervous system is still not completely ***,a natural killer cell-and T-lymphocyte-derived enzyme that mediates cytotoxicity,plays important roles in autoimmune diseases,infections and central nervous system trauma,such as spinal cord *** dissect the roles of this single component of the immune response to injury,we tested regeneration after femoral nerve injury in perforin-deficient(Pfp^(-/-))and wild-type control *** frame motion analysis showed better motor recovery in Pfp^(-/-)mice compared with control mice at 4 and 8 weeks after *** tracing of the motoneuron axons regrown into the motor nerve branch demonstrated more correctly projecting motoneurons in the spinal cord of Pfp^(-/-)mice compared with *** of regrown axons measured by g-ratio was more extensive in Pfp^(-/-)than in wild-type mice in the motor branch of the femoral ***^(-/-)mice displayed more cholinergic synaptic terminals around cell bodies of spinal motoneurons after injury than the injured *** histologically analyzed lymphocyte infiltration 10 days after surgery and found that in Pfp^(-/-)mice the number of lymphocytes in the regenerating nerves was lower than in wild-types,suggesting a closed blood-nerve barrier in Pfp^(-/-)*** conclude that perforin restricts motor recovery after femoral nerve injury owing to decreased survival of motoneurons and reduced myelination.

关键词： blood-nerve barrier femoral nerve injury locomotor recovery lymphocytes myelination NK-cells perforin reinnervation

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Characterization of Breast Cancer Radiofrequency Ablation Assisted with Magnetic Nanoparticles: In Silico and in Vitro Study

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Journal of Electromagnetic Analysis and Applications 2016年第1期8卷 1-7页

作者： Silverio Soto Alvarez Lidia Flor E. Huerta alma Verónica Vargas Jaime López Jesus Gabriel Silva César A. González Universidad del Ejército y FAM/EMGS Laboratorio Multidisciplinario de Investigación Mexico D.F. Mexico Instituto Politécnico Nacional Escuela Superior de Medicina SEPI Mexico D.F. Mexico

The effectiveness of breast cancer ablation by radiofrequency (RF) has been associated to the capacity of concentrating the electromagnetic energy in the tumor region, our group has proposed that this condition could be modulated by appropriate RF exposure cycle times as well as modification of tissue electrical conductivity. The aim of this work was to evaluate analytical and experimental optimal exposure cycle times to induce tissue ablation by RF assisted with magnetic nanoparticles. The study was conducted both analytically by multiphysics simulation of the induced currents in cancer tissue given a magnetron source and experimentally by the observation of hyperthermic effects induced in agar phantoms by a magnetron device by the use of RF assisted with magnetic nanoparticles. The temperature showed significant increases in short periods of time, and was clearly higher under the condition with nanoparticles. Appropriate RF exposure cycle times as well as modification of tissue electrical conductivity by magnetic nanoparticles seems suitable factors to modulate the electromagnetic energy in tumoral regions.

关键词： Breast Cancer Radiofrequency Ablation Multiphysics Simulation Magnetic Nanoparticles

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Cholinergic neuromuscular junctions in Brachionus calyciflorus and Lecane quadridentata(Rotifera:Monogononta)

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Journal of Coastal Life Medicine 2014年第5期 376-381页

作者： Ignacio Alejandro Pérez-Legaspi alma Lilián Guerrero-Barrera Iván JoséGalván-Mendoza JoséLuis Quintanar Roberto Rico-Martínez Graduate Studies and Research division Boca del Río Institute of TechnologyKm.12 Carr.Veracruz–Córdoba94290Boca del RíoVer.México Morphology department Basic Sciences CenterAguascalientes Autonomous UniversityAve.Universidad 940Aguascalientes20100México Confocal Microscopy Unit Experimental Services DepartmentCINVESTAV-ZacatencoNational Politechnic InstituteAve.Politécnico2508Zacatenco56000MéxicoD.F.México Pharmacology and Physiology department Aguascalientes Autonomous UniversityAve.Universidad 940Aguascalientes20100México Chemistry department Basic Sciences CenterAguascalientes Autonomous UniversityAve.Universidad 940Aguascalientes20100México

Objective:To identify the presence of joint muscular and cholinergic systems in two freshwater rotifer species,Brachionus calyciflorus and Lecane ***:The muscle actin fibers were stained with phalloidin-linked fluorescent dye,and acetylcholine was detected with Amplex Red Acetylcholine/Acetylcholinesterase Assay Kit,and then confocal scanning laser microscopy was ***:The musculature of Brachionus calyciflorus showed a pattern similar to other species of the same genus,while that of Lecane quadridentata was different from other rotifer genera described *** cholinergic system was determined by co-localization of both muscles and acetylcholine labels in the whole rotifer,suggesting the presence of neuromuscular ***:The distribution pattern of muscular and acetylcholine systems showed considerable differences between the two species that might be related to different adaptations to particular ecological *** confirmation of a cholinergic system in rotifers contributes to the development of potential neuro-pharmacological and toxicological studies using rotifers as model organism.

关键词： Acetylcholine Co-localization Confocal microscopy F-actin Rotifer

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Study of the second harmonic emission of glycine-sodium nitrate crystals at different pH

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Natural Science 2011年第4期3卷 319-322页

作者： R. A. Silva-Molina M. E. Alvarez-Ramos E. OrrantiA Borunda J. parra-Berumen E. Gallegos-Loya E. Torres-Moye d. Lardizabal A. duarte-Moller 不详

This work shows an optical and structural study of glycine sodium nitrate crystals. This study was supported with the respective X ray diffracttion and Second-Harmonic Generation signal detection by using a little variant to the Kurtz- Perry method. The goal of this work is to obtain the right pH that modifies the charge of glycine sodium nitrate system in order to obtain the best second harmonic emission. Furthermore with the change on the charge on the aminoacid, it is observed how it modifies the optical properties in the glycine sodium nitrate complex.

关键词： RAMAN GSN NLO SHG pKa

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divergence times and the reconstruction of a standardized taxonomic system based on multigene sequences:case from the genus Agaricus

Divergence times and the reconstruction of a standardized ta...

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中国菌物学会2015年学术年会

作者： Rui-Lin Zhao Jun-Liang Zhou Jie Chen Simona Margaritescu Santiago Sánchez-Ramírez Kevin d Hyde Philippe Callac Luis A.parra Guo-Jie Li Jean-Marc Moncalvo State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of Sciences Institute of Microbiology and Beijing Key Laboratory for Forest Pest Control Beijing Forestry University Institute of Excellence in Fungal Research Mae Fah Luang University School of Science Mae Fah Luang University department of Natural History Royal Ontario Museum department of Ecology and Evolutionary Biology University of Toronto Key Laboratory of Economic Plants and Biotechnology Kunming Institute of BotanyChinese Academy of Sciences INRA-UR1264 Mycologie et Sécurité des AlimentsCS 2003233882Villenave d'Ornon cedexFrance

the recognition of taxonomic ranks in current systematic and taxonomic research is lacking universally standardized criteria. Now molecular clock methods allow us to estimate divergence times based on multilocus sequences from extant and fossil agaric specimens. Agaricus（Agaricaceae, Agaricales） is a genus that contains many species of high commerial value. Recent research using ITS sequences discovered eleven new phylogenetic lineages. Proper taxonomic recognition and ranking of new lineages is essential to complete the natural systematics in this important group. In this study, we firstly conducted a preliminary ITS-only phylogenetic reconstruction using 745 ITS sequences（nearly 600 ITS sequences are new taxonomic contributions） including 59 from type specimens. Based on the preliminary ITS tree, 114 samples represented species from all twelve previously recognized Agaricus sections and seven of the eleven recently discovered clades were selected for producing LSU, tef-1α, and rpb2 sequences. Then the divergence time of the genus Agaricus and main clades within Agaricus were estamated using BEAST v1.8. Based on a phylogenetic consensus using a combination of multigene sequences genes（ITS, LSU, tef-1α and rpb2） and morphology, we propose a revised taxonomic system of Agaricus that considers the divergence time as a criterion for standardizing taxonomic rank, which can reflect evolutionary time while concomitantly redefining taxonomic groups based on phylogenetic relationships. The genus Agaricus was divided into five subgenera and 20 sections. Subgenera Minores and Spissicaules were created, subgenus Pseudochitonia was substantially emended, and circumscription of the subgenera Agaricus and Flavoagaricus was restricted to the members of sections Agaricus and Arvenses, respectively. Four new sections were created（Rubricosi, Crassispori, Flocculenti, and Amoeni）. Subsections Bohusia and Hondenses were raised to the sectional rank and placed into subgenus Pseudochiton

关键词： Agaricaceae divergence times multigene phylogenetics systematics taxonomy

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用熔解曲线法分析插入/缺失多态性和Y染色体SNPs多态性

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中国法医学杂志 2001年第4期16卷 214-217页

作者：叶健郑秀芬季安全 Esteban J parra donna M Sosnoski Mark d Shriver 宾夕法尼亚州立大学人类学系美国16801385 公安部物证鉴定中心北京100038

随着单核苷酸多态性SNPs (SingleNucleotidePolymorphisms)及插入 /缺失多态性Indels (Insertion/dele tion)的分型技术研究的深入 ,SNPs和Indels在法医学上的应用将受到深刻的影响。本文研究和探讨Indels的分型方法 ,通过测定扩增dNA... 详细信息

随着单核苷酸多态性SNPs (SingleNucleotidePolymorphisms)及插入 /缺失多态性Indels (Insertion/dele tion)的分型技术研究的深入 ,SNPs和Indels在法医学上的应用将受到深刻的影响。本文研究和探讨Indels的分型方法 ,通过测定扩增dNA片段在溶液中的溶解曲线图确定每个样品的基因型 ,称为溶解曲线Indels基因分型方法(McI/d)。溶解曲线图由被测样品dNA片段的特殊溶解温度组成 ,扩增结果直接由仪器分析不需要繁杂的PCR后期操作。

关键词：法医 dNA分型插入/缺失多态性 Y-SNPs多态性溶解曲线基因分型染色体

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塞内昔布和奈普生对肝硬化伴腹水的非氮质血症患者肾功能的影响

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世界核心医学期刊文摘（胃肠病学分册） 2005年第7期1卷 37-38页

作者： Clària J. Kent J.d. López- parra M. V. Arroyo 杨瑗 Liver Unit Institut de Malalties Digestives Hospital Clinic Villarroel 170 Barcelona 08036 Spain

Nonselective inhibition of cyclooxygenase (COX) by nonsteroidal anti- inflam matory drugs frequently induces renal failure in decompensated cirrhosis. Studie s in experimental cirrhosis suggest that selective inhibitors of the inducible i soform COX- 2 do not adversely affect renal function. However, very limited inf ormation is available on the effects of these compounds on renal function in hum an cirrhosis. This investigation consists of a double- blind, randomized, place bo- controlled trial aimed at comparing the effects of the selective COX- 2 in hibitor celecoxib (200 mg every 12 hours for a total of 5 doses) on platelet and renal function and the renal response to furosemide (40 mg intravenously) with those of naproxen (500 mg every 12 hours for a total of 5 doses) and placebo in 28 patients with cirrhosis and ascites. A significant reduction (P d urinary prostaglandin E2 excretion (3430 ± 430 to 2068 ± 549 pg/min) and suppression of the diuretic (urine volume: 5 61 ± 128 to 414 ± 107 mL/h) and natriuretic (urine sodium: 53 ± 13 to 34 ± 10 mEq/h) responses to furosemide were observed in the group of patients tre ated with naproxen but not in the other two groups. Naproxen, but not celecoxib or placebo, significantly inhibited platelet aggregation (72% ± 8% to 47% ± 8% , P d thromboxane B2 production (41 ± 12 to 14 ± 5 pg/mL, Pdicate that short- term administration of celecoxib does not impair platelet and renal function and the response to diu retics in decompensated cirrhosis.

关键词：塞内昔布氮质血症奈普生肾功能萘普生腹水患者安慰剂对照肾脏功能血小板功能尿钠增多

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Studying Some Brain and Ovarian Morphological Traits in diaglena spatulata Female Frogs Thriving in Conserved and Perturbed Habitats:A Preliminary Report and Its Implications for Amphibian Survival and Continuity

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Advances in Bioscience and Biotechnology 2014年第9期5卷 740-747页

作者： Esperanza Melendez-Herrera Bryan Victor Phillips-Farfan Fany Edith Bucio-Pina Noemi Ramirez-Arres Gabriel Gutierrez-Ospina Ireri Suazo-Ortuno Javier Alvarado-diaz alma Lilia Fuentes-Farias Laboratorio de Ecofisiologia Animal Departamento de ZoologiaInstituto de Investigaciones sobre los Recursos NaturalesUniversidad Michoacana de San Nicolas de HidalgoMoreliaMéxico Laboratorio de Nutricion Experimental Instituto Nacional de PediatriaSecretaria de SaludMexico Distrito FederalMexico Laboratorio de Biologia de Sistemas Departamento de Biologia Celular y FisiologiaInstituto de Investigaciones BiomedicasCoordinacion de Psicobiologia y NeurocienciasFacultad de PsicologiaUniversidad Nacional Autonoma de MexicoMexico Distrito FederalMexico Laboratorio de Ecologia Departamento de ZoologiaInstituto de Investigaciones sobre los Recursos NaturalesUniversidad Michoacana de San Nicolas de HidalgoMoreliaMexico Laboratorio de Herpetologia Departamento de ZoologiaInstituto de Investigaciones sobre los Recursos NaturalesUniversidad Michoacana de San Nicolas de HidalgoMoreliaMexico

Amphibians are thought to be highly susceptible to perturbed environments. However, recent studies show that many of them are successful inhabitants of disturbed, fragmented habitats. The source of this resilience is yet unclear, but it may be the byproduct of having a robust phenotype and/or the result of phenotypic plasticity. We then assessed the contribution of each by evaluating cytological features of two brain nuclei that modulate reproductive behavior and of the ovary, using female specimens of the frog diaglena spatulata prospering in conserved and disturbed areas of a tropical dry forest. Our results in the brain show that the medial amygdala, but not the preoptic area, had a reduced size in frogs collected in disturbed forests compared to specimens collected in conserved forests. Both brain nuclei displayed, however, neurons with a reduced size in frogs captured in disturbed forest patches. In contrast, ovarian cytological features were similar between groups. Our preliminary results lead us to propose that diaglena spatulata female specimens might combine robust ovary and plastic brain’s phenotypic traits to confront disturbed environments. This, however, is still a working hypothetical framework that needs to be experimentally confirmed.

关键词： Amphibians Reproductive Traits Amygdala Medial Preoptic Area Ovary

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Sensory neurons in the spinal cord of nominal female embryos in the marine turtle Lepidochelys olivacea respond to shifts in incubation temperature:implications for temperature dependent sex determination

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Advances in Bioscience and Biotechnology 2011年第1期2卷 1-7页

作者： Francisco Jimenez-Trejo Leonora Olivos-Cisneros Julieta Mendoza-Torreblanca Sofia diaz-Cintra Esperanza-Melendez-Herrera Armida Baez-Saldana Patricia Padilla Cortes Gabriel Gutierrez-Ospina alma Lilia Fuentes-Farias departamento de Biologia Facultad de QuimicaUniversidad Nacional Autonoma de MexicoMexico D.F.Mexico 04510 departamento de Biologia Celular y Fisiologia Instituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de MexicoMexico D.F.Mexico 04510 departamento de Neuroquimica Instituto Nacional de PediatriaMexicoDF.Mexico 04530 departamento de Neurobiologia del desarrollo y Neurofisiologia Instituto de NeurobiologiaUniversidad Nacional Autonoma de MexicoCampus UNAM-JuriquillaJuriquillaQueretaro Mexico AP.1-1141 Laboratorio de Invertebrados y Ecofisiologia Animal Facultad de BiologiaUniversidad Michoacana de San Nicolas de HidalgoMoreliaMichoacanMexico 58040 Unidad de Cromatografia Liquida de Alta Resolucion Instituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de MexicoMexico D.F.Mexico 04510

Gonadal determination in marine turtles depends on incubation temperature. The mechanisms that spark off this process remain unclear. Previously, we proposed that sensory nerves reaching the gonadal primordium in nominal female embryos of Lepidochelys (L) olivacea may sense and signal incubation temperature. These nerves could later trigger ovarian determination by releasing neurotransmitters in a code constructed based on the thermal information (Gutierrez-Ospina et al., Acetylcholinesterase-positive innervation is present at the undifferentiated stages of the sea turtle Lepidochelys olivacea embryo gonads: implications for temperature-dependent sex determination, J. Comp. Neurol. 410 (1999) 90-98). The hypothesis briefly described, however, has been recently refuted under weak theoretical grounds and experimental misinterpretations (see introduction). Here, we present preliminary results that show that nominal female embryos have sensory neurons located in the dorsal horn laminae I and II of the lumbar spinal cord that display increased c-Fos-like immuno-staining after being incubated either at 15°C or 50°C. Because these spinal neurons are the primary central target of dorsal root ganglion neurons that innervate the urogential crest, these observations keep open the possibility that gonadal sensory nerves indeed signal thermal information that could later be used to trigger or instruct ovarian specification in marine turtles.

关键词： Reptiles Ovarian determination c-Fos Incubation Temperature Sensory Neurons

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Quality of life, work productivity impairment and healthcare resources in inflammatory bowel diseases in Brazil

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World Journal of Gastroenterology 2019年第38期25卷 5862-5882页

作者： Rogerio Serafim parra Julio MF Chebli Heda MBS Amarante Cristina Flores Jose ML Parente Odery Ramos Milene Fernandes Jose JR Rocha Marley R Feitosa Omar Feres Antonio S Scotton Rodrigo B Nones Murilo M Lima Cyrla Zaltman Carolina d Goncalves Isabella M Guimaraes Genoile O Santana Ligia Y Sassaki Rogerio S Hossne Mauro Bafutto Roberto LK Junior Mikaell AG Faria Sender J Miszputen Tarcia NF Gomes Wilson R Catapani Anderson A Faria Stella CS Souza Rosana F Caratin Juliana T Senra Maria LA Ferrari department of Surgery and Anatomy Ribeirao Preto Medical SchoolUniversity of Sao PauloRibeirao PretoSP 14049-900Brazil Hospital Universitario da Universidade Federal do Piaui TeresinaPI 64049-550Brazil Carolina d Gonclves Isabella M GuimaraesUniversidade Federal do Rio de JaneiroRio de JaneiroRJ 21941-913Brazil Universidade do Estado da Bahia -SalvadorBA 41150-000Brazil department of Internal Medicine Botucatu Medical School at Sao Paulo State University(UNESP)BotucatuSP 18618-687Brazil Instituto Goiano de Gastroenterologia e Endoscopia digestiva Ltda GoianiaGO 74535-170Brazil Kaiser Clinica Sao Jose do Rio PretoSP 15015-110Brazil Escola Paulista de Medicina Sao PauloSP 04023-900Brazil UNIFESP Disciplina de GastroenterologiaSao PauloSP 04040-002Brazil Faculdade de Medicina do ABC Santo AndreSP 09060-870Brazil Faculdade de Medicina UFMG Belo HorizonteMG30130-100Brazil Inflammatory Bowel disease Center Federal University of Juiz de ForaJuiz de ForaMG 36036-247Brazil Takeda Pharmaceuticals Brazil Sao PauloSP 04709-011Brazil 不详 Hospital de Clinicas da Universidade Federal do Parana CuritibaPR 80060-900Brazil Hospital de Clinicas de Porto Alegre Porto Alegre-RS 90035-007Brazil Universidade Federal do Piaui TeresinaPI 64073-500Brazil Hospital de Clínicas da Universidade Federal do Parana CuritibaPR 80060-900Brazil CTI Clinical Trial&Consulting Services Lisbon 1070-274Portugal CMIP Centro Mineiro de Pesquisa Juiz de ForaMG 36010-570Brazil Hospital Nossa Senhora das Gracas CuritibaPR 80810-040Brazil

BACKGROUNd Inflammatory bowel diseases(IBd)have been associated with a low quality of life(QoL)and a negative impact on work productivity compared to the general *** about disease control,patient-reported outcomes(PROs),treatment patterns and use of healthcare resources is relevant to optimizing IBd *** To describe QoL and work productivity and activity impairment(WPAI),treatment patterns and use of healthcare resources among IBd patients in *** A multicenter cross-sectional study included adult outpatients who were previously diagnosed with moderate to severe Crohn’s disease(Cd)or ulcerative colitis(UC).At enrolment,active Cd and UC were defined as having a Harvey Bradshaw Index≥8 or a Cd Activity Index≥220 or calprotectin>200μg/g or previous colonoscopy results suggestive of inadequate control(per investigator criteria)and a 9-point partial Mayo score≥5,*** PRO assessment included the QoL questionnaires SF-36 and EQ-5d-5L,the Inflammatory Bowel disease Questionnaire(IBdQ),and the WPAI *** about healthcare resources and treatment during the previous 3 years was collected from medical ***-square,Fisher’s exact and Student’s t-/Mann-Whitney U tests were used to compare PROs,treatment patterns and the use of healthcare resources by disease activity(α=0.05).RESULTS Of the 407 patients in this study(Cd/UC:64.9%/35.1%,mean age 42.9/45.9 years,54.2%/56.6%female,38.3%/37.1%employed),44.7%/25.2%presented moderate-to-severe Cd/UC activity,respectively,at *** in median values for Cd/UC,respectively,the SF-36 physical component was 46.6/44.7 and the mental component was 45.2/44.2,the EQ-visual analog scale score was 80.0/70.0,and the IBdQ overall score was 164.0/*** to severe activity,female gender,being unemployed,a lower educational level and lower income were associated with lower QoL(Pdian work productivity impairment was 20%and 5%for Cd and UC patients,re

关键词： Inflammatory bowel disease Crohn’s disease Ulcerative colitis Quality of life Healthcare resources

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