Cholinergic neuromuscular junctions in Brachionus calyciflorus and Lecane quadridentata(Rotifera:Monogononta)

作     者：Ignacio Alejandro Pérez-Legaspi Alma Lilián Guerrero-Barrera Iván JoséGalván-Mendoza JoséLuis Quintanar Roberto Rico-Martínez 

作者机构：Graduate Studies and Research DivisionBoca del Río Institute of TechnologyKm.12 Carr.Veracruz–Córdoba94290Boca del RíoVer.México Morphology DepartmentBasic Sciences CenterAguascalientes Autonomous UniversityAve.Universidad 940Aguascalientes20100México Confocal Microscopy UnitExperimental Services DepartmentCINVESTAV-ZacatencoNational Politechnic InstituteAve.Politécnico2508Zacatenco56000MéxicoD.F.México Pharmacology and Physiology DepartmentAguascalientes Autonomous UniversityAve.Universidad 940Aguascalientes20100México Chemistry DepartmentBasic Sciences CenterAguascalientes Autonomous UniversityAve.Universidad 940Aguascalientes20100México 

出 版 物：《Journal of Coastal Life Medicine》 (海岸生命医学杂志（英文版）)

年 卷 期：2014年第5期

页      面：376-381页

学科分类：080901[工学-物理电子学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 080401[工学-精密仪器及机械] 0804[工学-仪器科学与技术] 0803[工学-光学工程] 

基　　金：Supported by Consejo Nacional de Ciencia y Tecnología(Conacyt)through the scholarship(Grant No.136453) Sistema Nacional de Investigadores(Grant No.49351) 

主　　题：Acetylcholine Co-localization Confocal microscopy F-actin Rotifer 

摘      要：Objective:To identify the presence of joint muscular and cholinergic systems in two freshwater rotifer species,Brachionus calyciflorus and Lecane ***:The muscle actin fibers were stained with phalloidin-linked fluorescent dye,and acetylcholine was detected with Amplex Red Acetylcholine/Acetylcholinesterase Assay Kit,and then confocal scanning laser microscopy was ***:The musculature of Brachionus calyciflorus showed a pattern similar to other species of the same genus,while that of Lecane quadridentata was different from other rotifer genera described *** cholinergic system was determined by co-localization of both muscles and acetylcholine labels in the whole rotifer,suggesting the presence of neuromuscular ***:The distribution pattern of muscular and acetylcholine systems showed considerable differences between the two species that might be related to different adaptations to particular ecological *** confirmation of a cholinergic system in rotifers contributes to the development of potential neuro-pharmacological and toxicological studies using rotifers as model organism.