Mice lacking perforin have improved regeneration of the injured femoral nerve

作     者：Igor Jakovčevski Monika von Düring David Lutz Maja Vulović Mohammad Hamad Gebhard Reiss Eckart Förster Melitta Schachner Igor Jakov?evski;Monika von Düring;David Lutz;Maja Vulovi?;Mohammad Hamad;Gebhard Reiss;Eckart F?rster;Melitta Schachner

作者机构：Institut für Anatomie und Klinische MorphologieUniversität Witten/HerdeckeWittenGermany Department of Neuroanatomy and Molecular Brain ResearchRuhr University BochumBochumGermany Department of AnatomyFaculty of Medical SciencesUniversity of KragujevacKragujevacSerbia Keck Center for Collaborative Neuroscience and Department of Cell Biology and NeuroscienceRutgers UniversityPiscatawayNJUSA 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2022年第17卷第8期

页      面：1802-1808页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the Li Kashing Foundation(to MS) the FoRUM grant F957N-2019 of the Ruhr-University Bochum(to DL) the Heinrich und Alma Vogelsang Stiftung(to IJ) 

主　　题：blood-nerve barrier femoral nerve injury locomotor recovery lymphocytes myelination NK-cells perforin reinnervation 

摘      要：The role that the immune system plays after injury of the peripheral nervous system is still not completely ***,a natural killer cell-and T-lymphocyte-derived enzyme that mediates cytotoxicity,plays important roles in autoimmune diseases,infections and central nervous system trauma,such as spinal cord *** dissect the roles of this single component of the immune response to injury,we tested regeneration after femoral nerve injury in perforin-deficient(Pfp^(-/-))and wild-type control *** frame motion analysis showed better motor recovery in Pfp^(-/-)mice compared with control mice at 4 and 8 weeks after *** tracing of the motoneuron axons regrown into the motor nerve branch demonstrated more correctly projecting motoneurons in the spinal cord of Pfp^(-/-)mice compared with *** of regrown axons measured by g-ratio was more extensive in Pfp^(-/-)than in wild-type mice in the motor branch of the femoral ***^(-/-)mice displayed more cholinergic synaptic terminals around cell bodies of spinal motoneurons after injury than the injured *** histologically analyzed lymphocyte infiltration 10 days after surgery and found that in Pfp^(-/-)mice the number of lymphocytes in the regenerating nerves was lower than in wild-types,suggesting a closed blood-nerve barrier in Pfp^(-/-)*** conclude that perforin restricts motor recovery after femoral nerve injury owing to decreased survival of motoneurons and reduced myelination.