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Species richness and density evaluation for plants with aggregated distributions:fixed *** area methods

Journal of Plant Ecology 2017年第5期10卷 765-770页

作者： Rita de Cássia Quitete Portela alexandra dos santos pires Maria Isabel Guedes Braz Eduardo Arcoverde de Mattos Departamento de Ecologia Instituto de BiologiaUniversidade Federal do Rio de JaneiroCaixa Postal 68020Ilha do FundãoRio de JaneiroRJ 21941-590Brasil Departamento de Ciências Ambientais Instituto de FlorestasUniversidade Federal Rural do Rio de JaneiroRod.BR 465Km 07Cidade UniversitáriaSeropédicaRio de JaneiroRJ 23890-000Brasil

Aims Knowledge of species richness is of prime importance to both basic and applied aspects of ecological ***,quantifying plant species richness in the tropics is potentially time-consuming because of high species *** species richness estimates are also frequently biased,because many rare species are not *** address these problems,the use of a variable area method has been proposed as an alternative to fixed area methods,but its appli-cability to plants with aggregated distributions has been questioned based on simulation *** use empirical data to compare the efficiency and accuracy of a variable area method and a fixed area method for estimating species richness,density and basal area for plants with aggregated distributions,using palms as a model *** Adult palms were sampled in twenty 10×30 m transects in an Atlantic Forest in Rio de Janeiro state,*** individuals were considered in the fixed area method,while in the variable area method only the first six adults in each transect were sampled;in this case,transect length was defined according to the distance of the sixth adult from the beginning of the *** fewer than six individuals were observed in a given transect,transect length was extended up to 50 m to search for additional *** efficiency of both methods was compared based on species rarefaction curves,using the chao 1 statistic(for abundance data).For each species we calculated mean density per transect and basal area,according to each sampling *** effort in terms of the number of individual plants and the area necessary to characterize maximum species richness in each sampling method,as well as mean time taken to sample a single transect,were compared as measures of *** findings An accurate estimate of species richness was achieved using both methods,but in the variable area method,a quarter of the number of individuals and half the area was sufficient

关键词： basal area biodiversity inventory palms taxon sampling

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Elevation transition of aquatic insects closely matches a thermal feature in the Yungas of Northwestern Argentina

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Journal of Mountain Science 2024年第2期21卷 433-448页

作者： alexandra BUITRAGO-GUACANAME Carlos MOLINERI Andrés LIRA-NORIEGA Daniel Andrés dos santos Neotropical Biodiversity Institute(CONICET-UNT) UNT-DomesHorco Molle S/N4107 TucumánArgentina CONACYT Research Fellow Network of Advanced Molecular StudiesInstitute of Ecology A.COld Road to Coatepec 351El HayaCP 91073XalapaVeracruzMexico Biostatistics Chair Faculty of Natural Sciences and IMLNational University of TucumánSan Miguel de TucumánMiguel Lillo 2054000 TucumánArgentina

Temperature is a key factor that shapes the distribution of *** knowledge about how species respond to temperature is relevant to devise strategies for addressing the impacts of climate *** insects are particularly vulnerable to climate change,yet there is still much to learn about their ecology and *** the Yungas ecoregion of Northwestern Argentina,cold-and warm-adapted species of the orders Ephemeroptera,Plecoptera,and Trichoptera(EPT)are segregated by *** modeled the ecological niche of South American EPT species in this region using available data and projected their potential distribution in geographic *** were grouped based on their ecogeographic similarity,and we analyzed their replacement pattern along elevation gradients,focusing on the ecotone where opposing thermal preferences *** this interface,we identified critical points where the combined incidence of cold and warm assemblages maximizes,indicating a significant transition *** found that the Montane Cloud Forest holds the interface,with a particularly greater suitability at its lower *** main axis of the interface runs in a N-S direction and falls between 14°C-16°C mean annual *** probability of a particular location within a basin being classified as part of the interface increases as Kira’s warmth index approaches a score around *** the interface is critical for defining the thermal limits of species distribution and designing biomonitoring *** in the location of thermal constants related to mountainous ecotones may cause vertical displacement of aquatic insects and vegetation *** have recognized significant temperature thresholds that serve as indicators of suitability for the *** global warming is anticipated to shift these indicators,we suggest using them to monitor the imprints of climate change on mountain ecosystems.

关键词： Austral Yungas Ecological Niche Model Ephemeroptera Kira’s warmth index Plecoptera Trichoptera

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Bioactive extracts of red seaweeds Pterocladiella capillacea and Osmundaria obtusiloba(Floridophyceae:Rhodophyta) with antioxidant and bacterial agglutination potential

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 363-370页

作者： Daniel Barroso de Alencar Fátima Cristiane Teles de Carvalho Rosa Helena Rebouas Daniel Rodrigues dos santos Kelma Maria dos santos pires-Cavalcante Rebeca Larangeira de Lima Bárbara Mendes Baracho Rayssa Mendes Bezerra Francisco Arnaldo Viana Regine Helena Silva dos Fernandes Vieira Alexandre Holanda Sampaio Oscarina Viana de Sousa Silvana Saker-Sampaio Laboratrio de Produtos Naturais Marinhos.Departamento de Engenharia de Pesca Universidade Federal do Ceará.Campus do Pici.Ar.Mister Hull.s/nCaixa Postal 6043 Laboratrio de Microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará.Av.da Abolicao 3207 Laboratrio de Microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará Laboratrio de Produtos Naturais Marinhos.Departamento de Engenharia de Pesca Universidade Federal do Ceará Laboratrio de Cromatografia Departamento de QuimicaUniversidade do Estado do Rio Grande do NorteCampus Universitário Central

Objective:To evaluate the antioxidant,antibacterial and bacterial cell agglutination activities of the hexane(Hex) and 70%ethanol(70%EtOH) extracts of two species of red seaweeds Pterocladiella capillacea(***) and Osmundaria ***:In vitro antioxidant activity was determined by DPPH radical scavenging assay,ferric-reducing antioxidant power assay,ferrous ion chelating assay,β-carotene bleaching assay and total phenolic content *** activity was tested using the method of disc diffusion on Mueller-Hinton *** ability of algal extracts to agglutinate bacterial cells was also ***:The 70%EtOH extract of the two algae showed the highest values of total phenolic content compared to the Hex *** results of DPPH for both extracts(Hex,70%EtOH) of Osmundaria obtusiloba(43.46%and 99.47%) were higher than those of ***(33.04%and 40.81%) at a concentration of 1 000 μg/*** for the ferrous ion chelating,there was an opposite behavior,extracts of *** had a higher *** extracts showed a low ferric-reducing antioxidant power,with optical density ranging from0.054 to *** activities of all extracts evaluated for β-carotene bleaching were above 40%.There was no antibacterial activity against bacterial strains ***,the extracts of both species were able to agglutinate bacterial Gram positive cells of Staphylococcus aureus and Gram negative cells of Escherichia coli,multidrug-resistant Salmonella and Vibrio *** This is the first report of the interaction between these algal extracts,rich in natural compounds with antioxidant potential,and Gram positive and Gram negative bacterial cells.

关键词： Bioactive compounds Antioxidant Bacterial agglutination Rhodophyta

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Candida albicans isolated from urine:Phenotypic and molecular identification,virulence factors and antifungal susceptibility

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Asian Pacific Journal of Tropical Biomedicine 2017年第7期7卷 624-628页

作者： Laura Wiebusch Adriana Araújo de Almeida-Apolonio Luana Mireli Carbonera Rodrigues Bruna de Paula Bicudo Danielly Beraldo dos santos Silva Danielle Ferreira Lonchiati Renata pires de Araujo Alexéia Barufatti Grisolia Kelly Mari pires de Oliveira Faculdade de Ciencias Biologicas e Ambientais Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Medicina Universidade Federal de Mato Grosso do SulCampo GrandeMSBrazil Faculdade de Ciencias da Saude Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Ciencias Agrarias e Veterinarias Universidade Estadual Paulista“Julio de Mesquita Filho”JaboticabalSPBrazil

Objective: To isolate Candida albicans(C. albicans) from the urine of hospitalized patients and assess the virulence factors and antifungal susceptibility profiles of the ***: Yeasts were identified using the chromogenic medium CHROMagar?, the VITEK~?2 system, hypertonic Sabouraud broth, tobacco agar, polymerase chain reaction,and DNA sequencing. The evaluated virulence factors were proteinase production,phospholipase production, and biofilm production on polystyrene. The broth microdilution technique was used to determine the minimum inhibitory ***: All yeasts isolated from urine were identified as C. albicans using both classical and molecular methods. Although 91.3% of the isolates showed no phospholipase activity, 56.5% showed strong proteinase activity and 91.7% produced biofilm. All microorganisms were sensitive to fluconazole, voriconazole and amphotericin B, but 56.5% of the yeasts showed resistance to ***: C. albicans isolates from urine have a high capacity for virulence and can be associated with infectious processes. Furthermore, the high percentage of isolates resistant to itraconazole is important because this antifungal agent is commonly used to treat fungal infections in the hospital environment.

关键词： Candidiasis Urinary tract Infection Virulence Antifungal susceptibility

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Controlled Reduction of Iron Content in Zirconiun Ore:A Case Study in Polyphase Mineralization,Pocs de Caldas Alkaline Plateau

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Journal of Geological Resource and Engineering 2018年第6期6卷 229-238页

作者： Ana Olivia Barufi Franco-Magalhas Daniel Francso de Godoy Leticia dos santos pires Instituto de Ciecia e Tecnologia Universidade Federal de AlfenasCampus Pocs de CaldasPocs de Caldas 37715-400Brazil Departamento de Petrologia e Metalogenia Instituto de Geociências e Ciências ExatasUniversidade Estadual PaulistaCampus Rio ClaroRio Claro 13506-900Brazil

In PCAC(Po?os de Caldas Alkaline Complex),in southeastern Brazil,it is observed a polyphase mineralization related to Zr-,U-,Th-,Mo-,and REE-enrichment due to hydromethermal processes which affected alkaline primary *** Zr-minerals were leached and concentrated as“caldasite”,a rock composed mainly by zircon and baddeleyite in different *** techniques of mineralogical characterization were applied and results indicated zircon,baddeleyite,magnetite and iron-oxyhydroxides,*** separation by WHIMS(Wet High-Intensity Magnetic Separation)was performed in order to indicate the efficiency for Fe-concentration removal for potential application in refractory industry.

关键词： Zircon baddeleyite caldasite Pocos de Caldas alkaline massif Brazil

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A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson’s disease potential biomarkers

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Translational Neurodegeneration 2020年第2期9卷 118-131页

作者： Sandra I.Anjo Patrícia Valério dos santos Luiza Rosado Graça Baltazar Inês Baldeiras Diana pires Andreia Gomes Cristina Januário Miguel Castelo-Branco Mário Grãos Bruno Manadas CNC-Center for Neuroscience and Cell Biology University of CoimbraCoimbraPortugal Faculty of Medicine University of CoimbraCoimbraPortugal Centro de Investigação em Ciências da Saúde(CICS-UBI) Universidade da Beira InteriorCovilhãPortugal Centro Hospitalar Cova da Beira E.P.ECovilhãPortugal Neurology Department Centro Hospitalar e Universitário de CoimbraCoimbraPortugal Coimbra Institute for Biomedical Imaging and Translational Research(CIBIT) CoimbraPortugal Biocant Biotechnology Transfer AssociationCantanhedePortugal Institute for Interdisciplinary Research University of Coimbra(IIIUC)CoimbraPortugal

Background The identification of circulating biomarkers that closely correlate with Parkinson’s Disease(PD)has failed several times in the ***,in this pilot study,a translational approach was conducted,allowing the evaluation of the plasma levels of two mitochondrial-related proteins,whose combination leads to a robust model with potential diagnostic value to discriminate the PD patients from matched *** The proposed translational approach was initiated by the analysis of secretomes from cells cultured under control or well-defined oxidative stress conditions,followed by the identification of proteins related to PD pathologic mechanisms that were altered between the two *** pipeline was further translated into the analysis of undepleted plasma samples from 28 control and 31 PD *** From the secretome analysis,several mitochondria-related proteins were found to be differentially released between control and stress conditions and to be able to distinguish the two ***,two mitochondrial-related proteins were found to be significantly changed in a PD cohort compared to matched ***,a linear discriminant model with potential diagnostic value to discriminate PD patients was obtained using the combination of these two *** proteins are associated with apoptotic mitochondrial changes,which may correspond to potential indicators of cell ***,one of these proteins,the VPS35 protein,was reported in plasma for the first time,and its quantification was only possible due to its previous identification in the secretome *** In this work,an adaptation of a translational pipeline for biomarker selection was presented and transposed to neurological diseases,in the present case Parkinson’s *** novelty and success of this pilot study may arise from the combination of:i)a translational research pipeline,where plasma samples are interrogated using knowledge previously ob

关键词： Mitochondrial-related proteins SWATH-MS Parkinson's disease Biomarker discovery Blood-biomarker Secretomes Oxidative stress

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Therapeutic Approaches to Post-Mastectomy Pain Syndrome

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Journal of Biosciences and Medicines 2023年第12期11卷 304-317页

作者： Vanessa Ferreira Dias Duarte Da Costa Giovanna Schwarz Mazzucca Carlos Augusto pires Zerbini Oscar César pires Camila dos santos Leite Laboratory of Pharmacology and Physiology Department of Medicine Taubaté University Taubaté Brazil

Background: Breast cancer is increasingly common in society and post-mastectomy pain is a striking condition associated with surgery, both intra and postoperatively. The post-mastectomy syndrome is characterized by the persistence of pain for more than 3 months after the procedure. It is mostly of neuropathic origin and is highly refractory to treatments, which vary from clinical to surgical measures and alternative techniques. In this context, it is essential to understand the possible approaches to these patients. Objective: Understand alternatives for pain management in patients undergoing mastectomy, especially in those in whom pain persists for more than 3 months. Methods: Systematic literature review, conducted in the Virtual Health Library databases includes: Lilacs, SciELO, Medline, PubMed and Cochrane between 2018 and 2023, restricted to articles in English with the descriptors: Mastectomy, chronic Pain, Nerve Blocks and Breast Cancer. 317 articles were found involving the descriptors presented, all in English. After reading the abstracts, 28 articles were selected. Results: This review analyzed 28 scientific studies that rigorously met the previously established characteristics in the sample selection. The synthesis included the following aspects: author/year of publication, article title, objective, type of study and database. Conclusion: It was concluded that, according to the analyzed studies, post-mastectomy pain is a problem that has increased its incidence and that needs the attention of health professionals. The use of antineuropathic medications, nerve blocks, fat grafting, lymph node transplantation, therapy, physical activity and acupuncture are some examples of therapeutic approaches for these women. Therefore, the team must assist the patients, seeking to provide a better prognosis, quality of life and comfort.

关键词： Mastectomy Chronic Pain Nerve Blocks Breast Cancer

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Tracheal Intubation Difficulties: Systematic Literature Review

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Journal of Biosciences and Medicines 2022年第5期10卷 95-104页

作者： Vanessa Ferreira Dias Duarte da Costa Mariana Ronchesel Barauna Camila dos santos Leite Oscar César pires Laboratory of Pharmacology and Physiology Medicine Department Taubaté University Taubaté Brazil Laboratory of Immunopharmacology and Molecular Biology S&atilde o Francisco University Medical School Bragan&ccedil a Paulista Brazil

Background: Tracheal intubation is a common procedure in medical practice, being performed mainly in surgical centers and emergencies in trauma patients. Objective: This systematic literature review aims to understand the main difficulties and complications related to tracheal intubation. Methods: Systematic literature review, conducted in the Virtual Health Library databases between 2016 and 2021, restricted to articles in English and Portuguese with the descriptors: Airway, Difficult intubation, and Complications. After reading the abstracts, 34 articles were found, and, after reading them, 9 articles were selected. Results: This review analyzed 9 scientific studies that rigorously met the previously established characteristics in the sample selection. The synthesis included the following aspects (author/year of publication, article title, objective, type of study, and database. Discussion: Several factors that influence the success of intubation were identified, such as technical, professional, and patient issues. In addition, measures to minimize the risks involving the procedure were highlighted. Conclusion: Tracheal intubation is a common procedure in surgical centers and emergencies. However, its risks, while low, should not be ignored. Unsuccessful procedures can occur and have consequences for the patient, such as trauma to the upper airway.

关键词： Airway Difficult Intubation Complications

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Effects of Dexamethasone, Clonidine, Tramadol and Nalbuphine on Fentanyl-Induced Hyperalgesia in Rats

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Journal of Biosciences and Medicines 2021年第12期9卷 87-97页

作者： Camila dos santos Leite Naira Correia Cusma Pelógia Eliane Stevanato Marília Hidalgo Uchôas Marta Helena Rovani pires Oscar César pires Laboratory of Immunopharmacology and Molecular Biology S&atilde o Francisco University Medical School Bragan&ccedil a Paulista Brazil Laboratory of Cell and Molecular Tumor Biology and Bioactive Compounds S&atilde o Francisco University Medical School Bragan&ccedil a Paulista Brazil Laboratory of Pharmacology and Physiology Medicine Department Taubaté University Taubaté Brazil Vera Cruz Hospital Campinas Brazil

Opioids are drugs used to alleviate pain. However, studies have demonstrated that these drugs can cause an increase in pain sensitivity, which is called opioid-induced hyperalgesia. The objective of this study was to describe the effects of dexamethasone, clonidine, tramadol and nalbuphine on fentanyl-induced hyperalgesia in rats. After obtaining approval from the Committee for the Ethical Use of Animals (CEUA), 36 male Wistar rats were divided into 6 groups: Group 1 (GCSSL) wherein the rats received 1 ml 0.9% saline solution in two injections;Group 2 (GFTSL), received fentanyl at a dose of 100 ug·kg-1 followed by 1 ml 0.9% saline solution via intraperitoneal;the remaining groups (3, 4, 5, 6) received fentanyl at a dose of 100 ug·kg-1 following doses via intraperitoneal: Group 3 (GFTDX), dexamethasone at a dose of 1.0 mg·kg-1;Group 4 (GFTCL), clonidine at a dose of 20 mg·kg-1;Group 5 (GFTTR), tramadol at a dose of 50 mg·kg-1, and Group 6 (GFTNB), nalbuphine at a dose of 5 mg·kg-1. Under general anestesia using isoflurane, the animals were submitted to a surgical incision. Hyperalgesia was evaluated by applying Von Frey filaments at 2 hours after the incision and on the 1st, 3rd and 5th days afterward. At 2 hours after the surgical procedure, there was lower intensity of pain in the fentanyl group (GFTSL) compared to the other groups, and on the fifth day there were no significant differences for pain intensity between groups. The results suggest the presence of fentanyl-induced hyperalgesia and efficacy in its reduction by dexamethasone, clonidine, tramadol and nalbuphine.

关键词： Hyperalgesia Fentanyl Dexamethasone Clonidine Tramadol Nalbuphine Rats

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Medical School Students’ Knowledge of Pain and Palliative Care: An Observational Study

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Journal of Biosciences and Medicines 2023年第4期11卷 39-49页

作者： Carolina Koutras Jacob Marina Perini Carlos Augusto P. Zerbini Marta H. R. pires Camila dos santos Leite Oscar César pires Laboratory of Pharmacology and Physiology Medicine Department Taubaté University Taubaté Brazil Vera Cruz Hospital Campinas Brazil Laboratory of Immunopharmacology and Molecular Biology S&atildeo Francisco University Medical School Bragan&ccedila Paulista Brazil Laboratory of Cell and Molecular Tumor Biology and Bioactive Compounds S&atildeo Francisco University Medical School Bragan&ccedila Paulista Brazil

Background: Pain and palliative care are a reality in daily routines of medical treatment. However, the theoretical-practical curricula of traditional medical school course still unsatisfactorily contemplate pain management, as well as the palliative care approach. Objective: To assess the knowledge of medical students about pain and palliative care, as well as to identify their perception of teaching these topics during hospitalization. Methods: A cross-sectional observational study, with a descriptive and exploratory approach, data collection for which was carried out between August and November 2020. The target population was medical students, who responded to an online survey of a quantitative, anonymous and follow-up nature. The survey study variables concerned knowledge about pain management and palliative care. Results: An expressive majority of academics showed difficulty in understanding the pathophysiology of pain related to prescribing drugs for pain management purposes, and all of them believe that it is necessary to acquire more knowledge about pain treatment. In parallel, only 9.3% report having received sufficient information regarding palliative care during medical school. Conclusion: The results suggest a certain lack of knowledge and insecurity among medical school students with respect to pain management and care for patients receiving palliative care. The didactical approach to this theme is still deficient in the medical curriculum and requires immediate improvement and new proposals that address the training of these professionals in a more specific and effective way.

关键词： Pain Palliative Care Medical Students Academic Institutions

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