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MicroRNA-149 Regulates Proliferation,Migration,and Invasion of Pituitary Adenoma Cells by Targeting ADAM12 and MMP14

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Current Medical Science 2022年第6期42卷 1131-1139页

作者： Zhuo ZHANG agnes schäfer Benjamin Voellger Jun-wen WANG Ting LEI Christopher Nimsky Jörg W.Bartsch Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Neurosurgery Philipps University MarburgBaldingerstrasseMarburg35033Germany Sino-German Neuro-Oncology Molecular Laboratory Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Center for Mind Brainand BehaviorHans-Meerwein-Strasse 6Marburg35032Germany

Objective Pituitary adenomas(PAs)can adapt an aggressive phenotype by invading adjacent brain structures with rapid cellular *** studies demonstrated that excessive expression of metalloproteases ADAM12 and MMP-14 is instrumental for the active proliferation and invasiveness of PA cells in vitro and of tumors in ***,the mechanisms regulating ADAM12 and MMP-14 expression in PAs remain *** Target gene prediction and transcriptomic profiling of invasive *** human PA samples were performed to identify miRNA species potentially involved in the regulation of ADAM12 and *** cellular analyses of miRNA functions,two mouse PA cell lines(AtT20 and TtT/GF)were transfected with miR-149-3p and miR-149-5p,*** effects of miR-149(3p and 5p)on expression levels of ADAM12 and MMP14 were determined by Western blotting followed by an analysis of proliferation and colony formation assays,scratch migration assays,and invasion *** A significant downregulation of miRNA-149 was observed in invasive *** PA(0.32 vs.0.09,Pferation and matrigel invasion,whilst the effect on cell migration was less *** strands of miRNA-149(3p and 5p)markedly reduced protein levels of ADAM12 and MMP-14 by at least 40%in both cell *** This study proved that the invasiveness of PA cells is,at least partly,regulated by miRNA-149-dependent expression of ADAM12 and MMP-14.

关键词： pituitary adenoma microRNA miRNA-149 ADAM12 MMP-14 invasion

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NECAB family of neuronal calcium-binding proteins in health and disease

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Neural Regeneration Research 2025年第5期20卷 1236-1243页

作者： Diones Bueno Michael K.E.schafer Sudena Wang Michael J.schmeisser Axel Methner Institute for Molecular Medicine University Medical Center of the Johannes Gutenberg-UniversityMainzGermany Department of Anesthesiology University Medical Center of the Johannes Gutenberg-UniversityMainzGermany Institute of Anatomy University Medical Center of the Johannes Gutenberg-UniversityMainzGermany

The N-terminal EF-hand calcium-binding proteins 1–3(NECAB1–3) constitute a family of predominantly neuronal proteins characterized by the presence of at least one EF-hand calcium-binding domain and a functionally less well characterized C-terminal antibiotic biosynthesis monooxygenase domain. All three family members were initially discovered due to their interactions with other proteins. NECAB1 associates with synaptotagmin-1, a critical neuronal protein involved in membrane trafficking and synaptic vesicle exocytosis. NECAB2 interacts with predominantly striatal G-protein-coupled receptors, while NECAB3 partners with amyloid-β A4 precursor protein-binding family A members 2 and 3, key regulators of amyloid-β production. This demonstrates the capacity of the family for interactions with various classes of proteins. NECAB proteins exhibit distinct subcellular localizations: NECAB1 is found in the nucleus and cytosol, NECAB2 resides in endosomes and the plasma membrane, and NECAB3 is present in the endoplasmic reticulum and Golgi apparatus. The antibiotic biosynthesis monooxygenase domain, an evolutionarily ancient component, is akin to atypical heme oxygenases in prokaryotes but is not wellcharacterized in vertebrates. Prokaryotic antibiotic biosynthesis monooxygenase domains typically form dimers, suggesting that calcium-mediated conformational changes in NECAB proteins may induce antibiotic biosynthesis monooxygenase domain dimerization, potentially activating some enzymatic properties. However, the substrate for this enzymatic activity remains uncertain. Alternatively, calcium-mediated conformational changes might influence protein interactions or the subcellular localization of NECAB proteins by controlling the availability of protein–protein interaction domains situated between the EF hands and the antibiotic biosynthesis monooxygenase domain. This review summarizes what is known about genomic organization, tissue expression, intracellular localization, interac

关键词： calcium EF hand mitochondria nervous system signal transduction

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Remains of the day:Biliary complications related to single-port laparoscopic cholecystectomy

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World Journal of Gastroenterology 2014年第3期20卷 843-851页

作者： Pierre Allemann Nicolas Demartines Markus schfer Department of Visceral Surgery University Hospital CHUV

AIM: To assesse the rate of bile duct injuries (BDI) and overall biliary complications during single-port laparoscopic cholecystectomy (SPLC) compared to conventional laparoscopic cholecystectomy (CLC).

关键词： Bile ducts Cholecystectomy Single port Single incision

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Accurate energy barriers for catalytic reaction pathways: an automatic training protocol for machine learning force fields

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npj Computational Materials 2023年第1期9卷 479-488页

作者： Lars L.schaaf Edvin Fako Sandip De Ansgar schäfer Gábor Csányi Engineering Laboratory University of CambridgeCB21PZ CambridgeUK BASF SE Carl-Bosch-Straße 3867056 LudwigshafenGermany

We introduce a training protocol for developing machine learning force fields(MLFFs),capable of accurately determining energy barriers in catalytic reaction *** protocol is validated on the extensively explored hydrogenation of carbon dioxide to methanol over indium *** the help of active learning,the final force field obtains energy barriers within 0.05 eV of Density Functional *** to the computational speedup,not only do we reduce the cost of routine in-silico catalytic tasks,but also find an alternative path for the previously established rate-limiting step,with a 40%reduction in activation ***,we illustrate the importance of finite temperature effects and compute free energy *** transferability of the protocol is demonstrated on the experimentally relevant,yet unexplored,top-layer reduced indium oxide *** ability of MLFFs to enhance our understanding of extensively studied catalysts underscores the need for fast and accurate alternatives to direct ab-initio simulations.

关键词： surface catalytic fields

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Thermophysical properties of the Ni-based alloy Nimonic 80A up to 2400 K

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Rare Metals 2006年第5期25卷 529-531页

作者： B.Wilthan R.Tanzer W.schützenhfer G.Pottlacher Institute of Experimental Physics Graz University of TechnologyPetersgasse 168010 GrazAustria Bhler Edelstahl GmbH Mariazellerstrasse 258605 KapfenbergAustria

Nimonic 80A is a nickel-chromium alloy which is strengthened by additions of titanium and aluminium. The alloy is used for high temperature, high strength applications. Wire shaped Nimonic 80A samples are resistively volume heated as part of a fast capacitor discharge circuit. Time resolved measurements with sub-μs resolution of current through the specimen are performed with a Pearson probe, voltage drop across the specimen is measured with knife-edge contacts and ohmic voltage dividers and the radiance temperature of the sample with a pyrometer. These measurements allow to determine heat of fusion as well as heat capacity and electrical resistivity at initial geometry of Nimonic 80A as a function of temperature in the solid and in the liquid phase up to 2400 K.

关键词： thermophysical properties Nimonic 80A resistivity enthalpy heat capacity latent heat

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Neural stem cell-based in vitro bioassay for the assessment of neurotoxic potential of water samples

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Journal of Environmental Sciences 2021年第3期33卷 72-86页

作者： Muhammad Irfan Masood Natalie Tamara Hauke Muhammad Jawad Nasim Muhammad Sarfraz Mahrukh Naseem Karl Herbert schäfer Division of Bioorganic Chemistry School of PharmacySaarland UniversityD-66123 SaarbrückenGermany Applied Life Sciences University of Applied Sciences Kaiserslautern66482 ZweibrückenGermany ENS Group University of Applied Sciences Kaiserslautern66482 ZweibrückenGermany Abwasserverband Bergstrasse Altau 1069469 WeinheimGermany Institute of Pharmaceutical Sciences University of Veterinary and Animal Sciences54000 LahorePakistan Department of Zoology University of BalochistanQuetta 87550Pakistan

Intensive agriculture activities,industrialization and growing numbers of wastewater treatment plants along river banks collectively contribute to the elevated levels of neurotoxic pollutants in natural water reservoirs across *** established an in vitro bioassay based upon neural stem cells isolated from the subventricular zone of the postnatal mouse to evaluate the neurotoxic potential of raw wastewater,treated sewage effluent,groundwater and drinking *** toxic potential of water samples was evaluated employing viability,proliferation,differentiation and migration *** found that raw wastewater could reduce the viability and proliferation of neural stem cells,and decreased the neuronal and astrocyte differentiation,neuronal neurite growth,astrocyte growth and cell *** sewage water also showed inhibitory effects on cell proliferation and *** results indicated that relatively high concentrations of nitrogenous substances,pesticides,mercuric compounds,bisphenol-A,and phthalates,along with some other pollutants in raw wastewater and treated sewage water,might be the reason for the neuroinhibitory effects of these water *** model successfully predicted the neurotoxicity of water samples collected from different sources and also revealed that the incomplete removal of contaminants from wastewater can be problematic for the developing nervous *** presented data also provides strong evidence that more effective treatments should be used to minimize the contamination of water before release into major water bodies which may be considered as water reservoirs for human usage in the future.

关键词： Neural stem cells Subventricular zone Neurotoxicity Bioassay Treated sewage effluents Pesticides

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Approximating multi-purpose AC Optimal Power Flow with reinforcement trained Artificial Neural Network

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Energy and AI 2022年第1期7卷 131-141页

作者： Zhenqi Wang Jan-Hendrik Menke Florian schäfer Martin Braun Alexander scheidler Department of Energy Management and Power System Operation University of KasselWilhelmshöher Allee 7334121 KasselGermany Fraunhofer Institute for Energy Economics and Energy System Technology Königstor 5934119 KasselGermany

Solving AC-Optimal Power Flow(OPF)problems is an essential task for grid operators to keep the power system safe for the use cases such as minimization of total generation cost or minimization of infeed curtailment from renewable DERs(Distributed Energy Resource).Mathematical solvers are often able to solve the AC-OPF problem but need significant computation *** neural networks(ANN)have a good application in function approximation with outstanding computational *** this paper,we employ ANN to approximate the solution of AC-OPF for multiple *** novelty of our work is a new training method based on the reinforcement learning concept.A high-performance batched power flow solver is used as the physical environment for training,which evaluates an augmented loss function and the numerical action *** augmented loss function consists of the objective term for each use case and the penalty term for constraints *** training method enables training without a reference OPF and the integration of discrete decision variable such as discrete transformer tap changer position in the constrained *** improve the optimality of the approximation,we further combine the reinforcement training approach with supervised training labeled by reference *** benchmark results show the high approximation quality of our proposed approach while achieving high computational efficiency on multiple use cases.

关键词： OPF Artificial neural network Reinforcement learning Grid planning and operation Grid congestion management

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First report of polymicrobial necrotizing fasciitis caused by Eggerthia catenaformis and Finegoldia magna

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World Journal of Emergency Medicine 2022年第4期13卷 326-329页

作者： Claudius Illg Jonas Kolbenschlag Ruth Christine schäfer Adrien Daigeler Sabrina Krauss Department of Hand Plastic and Reconstructive SurgeryBG Unfallklinik TuebingenEberhard Karls UniversityTuebingen 72076Germany

Dear editor,Necrotizing fasciitis is a relatively rare,rapidly advancing,infectious disease with high lethality.[1]It is characterized by subcutaneous and fascial tissue necrosis and can even extend to involve skin and muscle.[2]The initial spread along fascial planes with little visual evidence impedes the crucial early *** often report minor or major trauma preceding the infection,yet in some cases,the disease develops idiopathically.[3]Risk factors,including older age,diabetes,chronic renal failure,and obesity,predispose to impaired immunity and loss of physiological barriers.[2]

关键词： impaired immunity fas

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The protoplasts isolation,culture and shoots regeneration of broccoli(Brassica oleracea ***)

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华北农学报 1993年第S2期8卷 28-34页

作者：王怀名 A.schfer-Menuhr G.Mix-Wagner

Eight F1-hybrid cultivars of broccoli were *** obtained cell division,celled colonies and p-calli in 5 cultivars,roots and shoots regeneration in one *** leavesof propagated plantlets in vitro were cut into 1—2mm pieces,isolated with an enzyme solutioncontaining 2% cellulase and 1%macerase on a rotary shaker（50 rpm,21℃,3h,2500 lux light）,and purified with a 0.5M sucrose *** purified protoplasts were placed on a drop of 1%agarose.2—3 ml liquid medium was added around the agarose drops,and all of the cultures wereincubated at 25℃ under light（4000 lux）for 16 hours.3—5 days after isolation the cell divisionwas *** 7 days after incubation 4 multicellular colonies were *** 3—5 wksome p-calli were *** the p-calli were 2—3 mm in diameter it was transferred to asolidified *** they were developed to 1 cm in diameter they were transferred on a re-generation *** 5 months after incubation some roots and shoots grown from the calliwere

关键词： protoplasts culture shoots regeneration broccoli( Brassica oleracea var. italica )

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A simultaneous approach for integration of thermal energy storages in industrial processes using multiperiod heat integration

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Energy Storage and Saving 2022年第2期1卷 117-128页

作者： Simon Möhren Christian schäfer Jörg Meyer Hartmut Krause SWK E2 Institute for Energy Technology and Energy Management Hochschule Niederrhein University of Applied ScienceKrefeld47805Germany Institute of Thermal Engineering TU Bergakademie FreibergFreiberg09599Germany

In times of increasing global warming,enormous efforts are required to rapidly reduce greenhouse gas(GHG)*** to the EU’s target of climate neutrality by 2050 and the even more ambitious goal of becoming climate-neutral in Germany by 2045,it is necessary to systematically increase energy efficiency and decarbonize the industrial heat *** methods of heat integration can be used to exploit existing potentials for waste heat utilization and to integrate renewable technologies for heating and *** using a non-stationary,multiperiod approach,additional energy savings can be achieved by integrating a thermal energy storage(TES)that enables heat transportation over *** paper presents a simultaneous approach for thermal energy storage integration into multiperiod heat integration *** approach can be used to minimize energy demand,costs and CO 2 emissions and is demonstrated in two case *** case study 1,it is shown that the presented approach is capable of integrating a TES properly into a simple multiperiod heat integration problem with two *** case study 2,a simplified example from a cosmetics manufactory is *** total utility demand can be reduced by up to 44.3%due to TES integration and the energetic optimal storage size can be determined as 125 m *** savings are strongly dependent on the constellation of heat flows between the periods,on the temperature levels and on the storage *** reductions of energy demand,costs and CO 2 emissions can be achieved with TES being properly integrated into a suitable operating environment.

关键词： Multiperiod heat integration Thermal energy storage Waste heat utilization Heat exchanger network Energy efficiency

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