The protoplasts isolation,culture and shoots regeneration of broccoli(Brassica oleracea ***)
The protoplasts isolation,culture and shoots regeneration of broccoli(Brassica oleracea var. italica)*出 版 物：《华北农学报》 (Acta Agriculturae Boreali-Sinica)
年 卷 期：1993年第8卷第S2期
页 面：28-34页
核心收录：
主 题：protoplasts culture shoots regeneration broccoli( Brassica oleracea var. italica )
摘 要：Eight F1-hybrid cultivars of broccoli were *** obtained cell division,celled colonies and p-calli in 5 cultivars,roots and shoots regeneration in one *** leavesof propagated plantlets in vitro were cut into 1—2mm pieces,isolated with an enzyme solutioncontaining 2% cellulase and 1%macerase on a rotary shaker（50 rpm,21℃,3h,2500 lux light）,and purified with a 0.5M sucrose *** purified protoplasts were placed on a drop of 1%agarose.2—3 ml liquid medium was added around the agarose drops,and all of the cultures wereincubated at 25℃ under light（4000 lux）for 16 hours.3—5 days after isolation the cell divisionwas *** 7 days after incubation 4 multicellular colonies were *** 3—5 wksome p-calli were *** the p-calli were 2—3 mm in diameter it was transferred to asolidified *** they were developed to 1 cm in diameter they were transferred on a re-generation *** 5 months after incubation some roots and shoots grown from the calliwere
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