Neural stem cell-based in vitro bioassay for the assessment of neurotoxic potential of water samples

作     者：Muhammad Irfan Masood Natalie Tamara Hauke Muhammad Jawad Nasim Muhammad Sarfraz Mahrukh Naseem Karl Herbert Schäfer Muhammad Irfan Masood;Natalie Tamara Hauke;Muhammad Jawad Nasim;Muhammad Sarfraz;Mahrukh Naseem;Karl Herbert Schäfer

作者机构：Division of Bioorganic ChemistrySchool of PharmacySaarland UniversityD-66123 SaarbrückenGermany Applied Life SciencesUniversity of Applied Sciences Kaiserslautern66482 ZweibrückenGermany ENS GroupUniversity of Applied Sciences Kaiserslautern66482 ZweibrückenGermany Abwasserverband BergstrasseAltau 1069469 WeinheimGermany Institute of Pharmaceutical SciencesUniversity of Veterinary and Animal Sciences54000 LahorePakistan Department of ZoologyUniversity of BalochistanQuetta 87550Pakistan 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2021年第33卷第3期

页      面：72-86页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 0713[理学-生态学] 

基　　金：the Higher Education Commission(HEC),Pakistan(Ref.PD/OS-II/Batch-VI/Germany/2015/77159/17755) the University of Applied Sciences Kaiserslautern,Germany for providing financial support to execute the present project 

主　　题：Neural stem cells Subventricular zone Neurotoxicity Bioassay Treated sewage effluents Pesticides 

摘      要：Intensive agriculture activities,industrialization and growing numbers of wastewater treatment plants along river banks collectively contribute to the elevated levels of neurotoxic pollutants in natural water reservoirs across *** established an in vitro bioassay based upon neural stem cells isolated from the subventricular zone of the postnatal mouse to evaluate the neurotoxic potential of raw wastewater,treated sewage effluent,groundwater and drinking *** toxic potential of water samples was evaluated employing viability,proliferation,differentiation and migration *** found that raw wastewater could reduce the viability and proliferation of neural stem cells,and decreased the neuronal and astrocyte differentiation,neuronal neurite growth,astrocyte growth and cell *** sewage water also showed inhibitory effects on cell proliferation and *** results indicated that relatively high concentrations of nitrogenous substances,pesticides,mercuric compounds,bisphenol-A,and phthalates,along with some other pollutants in raw wastewater and treated sewage water,might be the reason for the neuroinhibitory effects of these water *** model successfully predicted the neurotoxicity of water samples collected from different sources and also revealed that the incomplete removal of contaminants from wastewater can be problematic for the developing nervous *** presented data also provides strong evidence that more effective treatments should be used to minimize the contamination of water before release into major water bodies which may be considered as water reservoirs for human usage in the future.