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化学不育剂—环丙醇类衍生物控制鼠害:III.灭鼠现场试验

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四川动物 2003年第4期22卷 215-217页

作者：王酉之马林陈东平杨世枣四川省疾病预防控制中心成都610031 成都市疾病预防控制中心四川新洁灵医疗卫生保健品有限公司

20 0 0年 4月至 2 0 0 1年 6月进行了为期 15个月使用化学不育剂———环丙醇类衍生物控制鼠害的现场试验。鼠密度于试验 5个月后由初始的 6 3.8%下降为 4 2 % ,11个月后为 4 % ,控制率高达 93.7%。检测幼体足迹 ,由开始的 2 5 .4 % 11... 详细信息

20 0 0年 4月至 2 0 0 1年 6月进行了为期 15个月使用化学不育剂———环丙醇类衍生物控制鼠害的现场试验。鼠密度于试验 5个月后由初始的 6 3.8%下降为 4 2 % ,11个月后为 4 % ,控制率高达 93.7%。检测幼体足迹 ,由开始的 2 5 .4 % 11个月后下降为 0。半年后检查雄性精子浓度、活精子数和精子死亡率等 3项指标 ,试验组与对照组差异显著 ,表明雄性生殖系统均受损伤。试验表明 ,该不育剂使鼠类密度较长期控制在不危害水平 ,次年繁殖期密度反而下降。

关键词：化学不育剂环丙醇类衍生物控制鼠害

Expression and Comparative Analysis of Genes Encoding Outer Membrane proteins LipL21,LipL32 and OmpL1 in Epidemic Leptospires

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Acta Biochimica et Biophysica Sinica 2005年第10期37卷 7+650-656页

作者： Xiang-Yan ZHANG~(1#) Yang YU~(2,3#) ping HE~1 Yi-Xuan ZHANG~(2,3) Bao-Yu HU~1 Yang YANG~1 Yi-Xin NIE~4 Xiu-Gao JIANG~4 Guo-ping ZHAO~(2,3) Xiao-Kui GUO~(1*) 1 Department of Medical Microbiology and parasitology,Shanghai Jiaotong University,Shanghai 200025,china 2 research center of Biotechnology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,china 3 Chinese National Human Genome center at Shanghai,Zhangjiang High Tech park,Shanghai 201203,china 4 Institute for Infectious disease control and prevention,Chinese center for disease control and prevention,Beijing 102206,china DepartmentofMedicalMicrobiologyandparasitology ShanghaiJiaotongUniversityShanghai200025China

Leptospiral outer membrane proteins (OMps) are highly conserved in different species,andplay an essential role in the development of new immunoprotection and serodiagnosis *** genesencoding LipL21,LipL32 and OmpL1 were cloned from the complete genome sequence of Leptospirainterrogans serovar lai strain Lai and expressed in *** comparison analysis revealed that the threegenes were highly conserved among distinct epidemic leptospires,including three major epidemic speciesLeptospira interrogans,Leptospira borgpetersenii and Leptospira weilii,in *** analysis wasfurther performed to scrutinize 15 epidemic Leptospira reference strains using the antisera of the *** immunoblot assay and reverse transcription-polymerase chain reaction demonstrated that thesethree OMps were conservatively expressed in pathogenic *** strains and other pathogenic ***,the use of these recombinant OMps as antigens in enzyme-linked immunosorbent assay (ELISA)for serodiagnosis of leptospirosis was *** recombinant LipL32 and OmpL1 proteins showed ahigh degree of ELISA reactivity with sera from patients infected with *** strain Lai and otherpathogenic *** results may contribute to the identification of candidates for broad-rangevaccines and immunodiagnostic antigens in further research.

关键词： outer membrane protein expression comparative analysis epidemic leptospire

Meta-analysis of the relationship between Epstein-Barr virus infection and clinicopathological features of patients with gastric carcinoma

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Science china(Life Sciences) 2010年第4期53卷 524-530页

作者： LI ShuYing1,2, DU HaiJun3, WANG Zhan3, ZHOU Ling3, ZHAO XiaoYu1 & ZENG Yi3 1 College of Life Sciences, Hebei University, Baoding 071002, china 2 Department of pathogenic Biology, Faculty of Biological Science, North china Coal Medical College, Tangshan 063000, china 3 National Institute for Viral disease control and prevention, Chinese center for disease control and prevention (Chinese CDC), State Key Laboratory for Infectious disease prevention and control (SKLID), Beijing 100052, china 1. College of Life Sciences Hebei University Baoding 071002 China2. Department of Pathogenic Biology Faculty of Biological Science North China Coal Medical College Tangshan 063000 China3. National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention (Chinese CDC) State Key Laboratory for Infectious Disease Prevention and Control (SKLID) Beijing 100052 China

Epstein-Barr virus (EBV) infection has been causally associated with occurrence of many malignant neoplasms. EBV-encoded small rNAs (EBErs) have been detected from about 10% of gastric carcinoma tissue cells, suggesting that EBV infection is associated with the development of gastric carcinoma. The present study pooled the data from the papers concerning EBV-related gastric cancers and performed a meta-analysis of 22 research papers. Among these papers, a total of 5475 cases with gastric cancer were enrolled, of whom 411 cases were found EBV-positive, with the EBV-positive rate being 7.5%. Among the EBV-positive gastric cancer cases, the detection rate was 11.1% in males and 3.0% in females. Compared with EBV-negative gastric cancer, EBV-positive gastric cancer had less lymph node metastasis. Based on the histological typing, of the EBV-positive gastric cancers, the diffuse type was 8.1%, and intestinal type was 8.0%. The examined specimen types included stored paraffin blocks and fresh surgically removed specimens, their EBV positive rates were 7.9% and 6.5% respectively. In terms of geographical distribution, the detection rate of EBV-positive gastric cancer was 9.4% in America, 6.1% in Asia and 9.1% in Europe. Meta-analysis showed that EBV infection occurred only in gastric cancer tissue cells and was significantly associated with the patients’ gender, lymph node metastases, and the location where tumor tissue generated and geographical distribution (ppatients’ histological types of tumor and the types of specimens (p>0.05). These results suggested that EBV-positive gastric cancer has distinct clinicopathological features.

关键词： Epstein-Barr virus gastric carcinoma meta-analysis in situ hybridization

Determination of 23 β_2-agonists and 5 β-blockers in animal muscle by high performance liquid chromatography-linear ion trap mass spectrometry

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Science china Chemistry 2010年第4期53卷 832-840页

作者： WU YongNing1,2, MIAO Hong1, FAN Sai1,2 & ZHAO YunFeng1,2 1 Institute of Nutrition and Food Safety, Chinese Centre for disease control and prevention, Beijing 100021, china 2 College of Veterinary Medicine, china Agricultural University, Beijing 100193, china 1. Institute of Nutrition and Food Safety Chinese Centre for Disease Control and Prevention Beijing 100021 China2. College of Veterinary Medicine China Agricultural University Beijing 100193 China

A high performance liquid chromatography-linear ion trap mass spectrometry method using isotope dilution technique has been developed for the simultaneous determination of 23 β2-agonists and 5 β-blockers in animal muscle tissues. pork and chicken muscle samples were acid hydrolyzed and extracted with 5% trichloracetic acid in water, and then cleaned up using MCX solid phase extraction (SpE) cartridge. Methanol and 0.1% formic acid were used as mobile phases for gradient elution. A Waters AtlantisT3 column was used for separation. ESI positive ion scan mode was used with selective reaction monitoring. 9 β2-Agonists labeled by the deuterium isotope were used as internal standards for quantification. The linear ranges of 23 β2-agonists and 5 β-blockers were 5-200 μg/L, the coefficient of correlation was not less than 0.995, and the limit of detection for each compound in the muscle tissue was below 0.2 μg/kg. The recoveries of each compound in the spiked samples at three levels 5, 10, 20 μg/kg were in the range of 47.3%-123.7%, and the relative standard deviations were in the range of 3.2%-25.7%. The developed method is sensitive and specific for the determination of β2-agonists and β-blockers in pork and chicken muscle samples.

关键词： β2-agonists β-blockers muscle determination HpLC-ITMS

Treat to target in Crohn’s disease:A practical guide for clinicians

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World Journal of Gastroenterology 2024年第1期30卷 50-69页

作者： Ashish r Srinivasan Department of Gastroenterology Austin HealthVictoriaMelbourne 3083Australia Department of Gastroenterology Eastern HealthVictoriaMelbourne 3128Australia Department of Medicine University of MelbourneVictoriaMelbourne 3052Australia

A treat-to-target(T2T)approach applies the principles of early intervention and tight disease control to optimise long-term outcomes in Crohn's *** Selecting Therapeutic Targets in Inflammatory Bowel disease(STrIDE)-II guidelines specify short,intermediate,and long-term treatment goals,documenting specific treatment targets to be achieved at each of these *** appraisal of Crohn’s disease activity against pre-defined treatment targets at these timepoints remains central to determining whether current therapy should be continued or *** treatment targets in Crohn’s disease comprise combination clinical and patient-reported outcome remission,in conjunction with biomarker normalisation and endoscopic *** the STrIDE-II guidelines endorse the pursuit of endoscopic healing,clinicians must consider that this may not always be appropriate,acceptable,or achievable in all *** underscores the need to engage patients at the outset in an effort to personalise care and individualise treatment *** use of non-invasive biomarkers such as faecal calprotectin in conjunction with cross-sectional imaging techniques,particularly intestinal ultrasound,holds great promise;as do emerging treatment targets such as transmural *** randomised clinical trials,namely,CALM and STArDUST,have evaluated the efficacy of a T2T approach in achieving endoscopic endpoints in patients with Crohn’s *** from these studies reflect that patient subgroups and Crohn’s disease characteristics likely to benefit most from a T2T approach,remain to be ***,outside of clinical trials,data pertaining to the real-world effectiveness of a T2T approach remains scare,highlighting the need for pragmatic real-world *** the obvious promise of a T2T approach,a lack of guidance to support its integration into real-world clinical practice has the potential to limit its *** highlights the need to describe st

关键词： Treat to target Inflammatory bowel disease Crohn’s disease Treatment targets Endoscopic remission Transmural healing Time to response Intestinal ultrasound

Quantitative analysis of virulent duck enteritis virus loads in experimentally infected duckings

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Quantitative analysis of virulent duck enteritis virus loads...

Apoptosis Induced by Duck Virual Swollen Head Haemorrhagic disease Virus in Duck Embryo Fibroblasts Cell

第四届第九次全国学术研讨会暨饲料和动物源食品安全战略论坛

作者： Yang Xiaoyan Cheng Anchun Wang Mingshu Qi Xuefeng Guo Yufei Jia renyong (research center of Avian disease,College of animal Science and Veterinary Medicine of sichuan Agricultural University,Ya’an,sichuan,625014,china.Key Laboratory of animal disease and Human Health of sichuan province,Ya’an,sichuan,625014,china.College of Life Science and Chemistr of Dali University,Da’li,Yunnan,671000,china)

To understand better the pathogenesis of duck virus enteritis (DVE),the levels of viral DNA in various tissues of duckling during acute stage of virulent duck enteritis virus (DEV) infection were investigated by using quantitative real-time *** results show that the viral levels of DEV in systemic organs have a close correlation with the progression of *** rapid dissemination of virulent DEV to systemic organs and active replication in lymphoid tissues at the early phase of acute infection accelerate the progression of *** levels of viral DNA increased sharply soon after develop clinical signs of disease,and the extent of increase and the final magnitude of DEV DNA load in various tissues of ducklings immediately following the exhibition of clinical signs may be a critical determinant of the outcome of DEV *** relatively high level of DEV infection and replication in bursa and intestine tissues of dead ducklings most likely reflects the abundance of target epithelial and lymphoid cells in these tissues,and therefore play a key role in the pathogenesis of acute DVE manifested as severe lesions on the lymphoid tissues and small intestine.

关键词： Duck enteritis virus Quantitation pathogenesis

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Apoptosis Induced by Duck Virual Swollen Head Haemorrhagic D...

Study of Cytoskeletal Changes Induced by Okadaic Acid in HL-7702 Liver Cells and Development of a Fluorimetric Microplate Assay for Detecting Diarrhetic Shellfish poisoning

第四届第九次全国学术研讨会暨饲料和动物源食品安全战略论坛

作者： ZHANG Na~1 CHENG An-chun~(1,2) WANG Ming-shu~(1,2) LI Chuan-feng~1 CHEN Xiao-yue~1 (1.Avian disease research center,College of Veterinary Medicine,sichuan Agricultural University,Ya’an 625014, sichuan,china 2.Key Laboratory of animal disease and Human Health of sichuan,Ya’an 625014,sichuan,china)

After the replication of duck viral swollen head haemorrhagic disease virus(DSHDV)in duck embryo fibroblasts(DEFs)was successfully *** apoptosic changes were detected after DSHDV infection at different time by light microscope,transmission electron microscopy(TEM),DNA Ladder and Annexin V-FITC/pI stained fluorescence-activated cell sorter(FACS).The results show that DSHDV can induce DEFs to undergo the apoptosis change *** morphological apoptotic features including cell shrinkage and condensation,margination of nuclear chromation were observed under light microscope and the formation of apoptotic bodies by *** ladder was shown by DNA fragment analysis at 24-144h post *** percentage of apoptotic cells were analyzed quantitatively by using FACS.

关键词： duck viral swollen head haemorrhagic disease virus(DSHDV) duck embryo fibroblasts(DEFs) apoptosis

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Study of Cytoskeletal Changes Induced by Okadaic Acid in HL-...

Implication of reported Viral Hepatitis Incidence rate Change in Hubei province, china, between 2004-2010

广东省环境诱变剂学会、广东省预防医学会卫生毒理专业委员会2010年学术会议

作者： Haiyan Huang,Aijun Huang,Zhixiong Zhuang,Wei Huang,Yingbin Fu,Chaoqiong peng,Jianjun Liu~* (Toxicology Laboratory,Shenzhen center for disease control and prevention,Shenzhen,Guangdong,p.r.china)

Diarrhetic shellfish poisoning（DSp） is a gastrointestinal illness with symptoms such as diarrhea,nausea, vomiting,headache,chills and moderate to severe abdominal *** has been recognized as a worldwide public health problem,causing great concern to the shellfish *** of DSp in shellfish is an unpredictable phenomenon that necessitates the implementation of a widespread collection and thorough monitoring program for mollusk ***,development of accurate analytical protocols for the rapid determination of toxicity levels would be *** the current study we investigated eytoskeletal changes induced by okadaic acid in HL-7702 Liver Cells and developed a new cytotoxicity assay for detection and quantitation of *** assay is based on fluorometric of F-actin depolymerization induced by okadaic acid（OA） compounds in HL-7702 liver cell *** measurable range of OA was 2.5～40 nmol/*** detection limit of the F-actin assay for OA was 2.01μg/100g muscles in shellfish *** performance of this assay has been evaluated by comparative analysis of shellfish samples by the fluorescent assay,mouse bioassay,and ELISA assay. Comparison of the results by all three methods revealed excellent consistency,the results of fluorescent assay were in significant correlation with ELISA assay（r=0.830）.Examination of F-actin assay is very convenient, rapid,and sensitive,which can be used to quantify the amount of OA in shellfish samples.

关键词： Diarrhetic shellfish poisoning(DSp) okadaic acid(OA) F-actin ELISA assay Mouse bioassay

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2012年第3期32卷 428-433页

作者：胡樱宇传华陈邦华王雷 Department of Epidemiology and Biostatistics School of Public Health of Wuhan University Wuhan University Global Health Institute Institute of Infectious disease control and prevention Wuhan Center for Disease Control and Prevention Institute of Infectious disease control and prevention Hubei Center for Disease Control and Prevention

This study examined the change of reported incidence rate for viral hepatitis in Hubei province, china, between 2004 to 2010 to provide scientific evidence for viral hepatitis control. reported viral hepatitis infection cases were queried from Centre for disease control of Hubei province, china. The incidence of viral hepatitis A decreased steadily across the study period. Viral hepatitis B composed 85% of the viral hepatitis cases. When reported incidence rates for chronic hepatitis B increased, the rates of acute and unclassified cases dropped from 2005 to 2010. The reported viral hepatitis B incidence rate for males was around 1.5-2 times higher than for females. The average annual percentage change of reported viral hepatitis B incidence rates was 4%. The same index for viral hepatitis C was 28%. The reported viral hepatitis B incidence rate of people under 20 years old declined over the period. This decrease was mainly attributed to the recent implementation of vaccination plan. reported incidence rate of viral hepatitis E also rose in those years. Having a better understanding on reported incidence rates of the present surveillance system is important for developing strategies for further prevention of viral hepatitis. In addition, the data showed that a surveillance system that differentiates new and former infected cases will be more effective in providing evidence for disease control.

关键词： viral hepatitis reported incidence rate vaccination prevention