Expression and Comparative Analysis of Genes Encoding Outer Membrane Proteins LipL21,LipL32 and OmpL1 in Epidemic Leptospires

作     者：Xiang-Yan ZHANG~(1#) Yang YU~(2,3#) Ping HE~1 Yi-Xuan ZHANG~(2,3) Bao-Yu HU~1 Yang YANG~1 Yi-Xin NIE~4 Xiu-Gao JIANG~4 Guo-Ping ZHAO~(2,3) Xiao-Kui GUO~(1*) 1 Department of Medical Microbiology and Parasitology,Shanghai Jiaotong University,Shanghai 200025,China 2 Research Center of Biotechnology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 3 Chinese National Human Genome Center at Shanghai,Zhangjiang High Tech Park,Shanghai 201203,China 4 Institute for Infectious Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China 

作者机构：DepartmentofMedicalMicrobiologyandParasitologyShanghaiJiaotongUniversityShanghai200025China 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2005年第37卷第10期

页      面：7+650-656页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：This work was supported by the grants from the National High Technology Research and Development Program of China (No.2003AA223030) the National Natural Science Foundation of China and Shanghai Leading Academic Discipline Project (No.T0206) 

主　　题：outer membrane protein expression comparative analysis epidemic leptospire 

摘      要：Leptospiral outer membrane proteins (OMPs) are highly conserved in different species,andplay an essential role in the development of new immunoprotection and serodiagnosis *** genesencoding LipL21,LipL32 and OmpL1 were cloned from the complete genome sequence of Leptospirainterrogans serovar lai strain Lai and expressed in *** comparison analysis revealed that the threegenes were highly conserved among distinct epidemic leptospires,including three major epidemic speciesLeptospira interrogans,Leptospira borgpetersenii and Leptospira weilii,in *** analysis wasfurther performed to scrutinize 15 epidemic Leptospira reference strains using the antisera of the *** immunoblot assay and reverse transcription-polymerase chain reaction demonstrated that thesethree OMPs were conservatively expressed in pathogenic *** strains and other pathogenic ***,the use of these recombinant OMPs as antigens in enzyme-linked immunosorbent assay (ELISA)for serodiagnosis of leptospirosis was *** recombinant LipL32 and OmpL1 proteins showed ahigh degree of ELISA reactivity with sera from patients infected with *** strain Lai and otherpathogenic *** results may contribute to the identification of candidates for broad-rangevaccines and immunodiagnostic antigens in further research.