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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Li Shuqing~1,Li Wenwen~2,Chen Zhifei~1,Li Jian~2,Chen Shaohong~3,Zhang Yongnian~3 Huang Weiyi~2 Wang Qiaoquan~1 1.shanghai Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of china,shanghai 200135 2.College of Animal Science and Technology,Guangxi University,Nanning 530005 3.chinese center for disease Control and Prevention,national Institute of Parasitic diseases, shanghai 200025

Objective To inspect the third stage larvae of Gnathostoma in imported Monopterus albus,and identify its species..Method 10 batches of imported Monopterus albus to shanghai from abroad were detected for nematode Gnathostoma from January 2010 to March *** 52 Monopterus albus,3～10/batch,From Philippines(25),Indonesia(24) and Bangladesh(3),dissect each Monopterus albus,then minced,protease digestion,the suspension was filtered with 10 mesh copper screen,at the last take the precipitation of the *** the complete parasites under the stereoscope,morphological identification,and calculate the infection rate and average *** extracted genomic DNA of Gnathostoma,amplified internal transcribed spacer region 2 and cytochrome c oxidase subunit 1 by PCR,then Gel electrophoresis and *** sequences were aligned with corresponding sequences in *** The third stage larvas of nematode Gnathostoma were detected in Monopterus albus from Indonesia and Philippines,infection rates were 36.0%(9/25) and 50.0% (12/24),average infectiosity were 7.77(70/9) and 2.83(34/12).There wasn’t Gnathostoma in imported Monopterus albus from *** the microscope,the larvas have one cephalic bulb,4 rings hooklets on the cephalic bulb,the body surface with cross striations and small spines,the front of the body spines were obviously and density,the rear spines getting small and *** had 1 cervical papillae,4 cervical *** characteristics was similar to Gnathostoma *** results showed,ITS-2 and cox1 PCR products were 647 bp and 441 bp,with the expected *** alignment analysis showed that,two PCR products had 99%～100%consistency with Gnathostoma spinigerum ITS-2(GenBank accession number AB181155 and Z97175) and coxl (GenBank accession number AY501388,AB180099 and AB551552).Conclusion All of the Gnathostoma detected in imported Monopterus albus from Philippines and Indonesia were Gnathostoma spinigerum.

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冠状动脉CT血管成像数据标注和质量控制专家共识

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中华放射学杂志 2024年第1期58卷 25-33页

作者：中华医学会放射学分会心胸学组中国医师协会放射医师分会心血管学组中国食品药品检定研究院刘士远金征宇郑敏文吕滨萧毅李小虎卢洁张龙江王浩张佳胤不详海军军医大学第二附属医院放射诊断科上海200003 中国医学科学院北京协和医学院北京协和医院放射科北京100730 空军军医大学西京医院放射诊断科西安710032 中国医学科学院北京协和医学院国家心血管病中心阜外医院放射影像科北京100037 安徽医科大学第一附属医院医学影像科首都医科大学宣武医院放射与核医学科解放军东部战区总医院放射诊断科中国食品药品检定研究院医疗器械检定所上海市第一人民医院放射影像科

冠状动脉CT血管成像(CCTA)是冠状动脉疾病诊断的首选影像检查。使用人工智能技术对CCTA数据进行图像重组、斑块评估和血管分析,是人工智能医疗器械发展的重要方向,为临床机构提供了全新的影像后处理和诊断方式。影像人工智能产品的生产... 详细信息

冠状动脉CT血管成像(CCTA)是冠状动脉疾病诊断的首选影像检查。使用人工智能技术对CCTA数据进行图像重组、斑块评估和血管分析,是人工智能医疗器械发展的重要方向,为临床机构提供了全新的影像后处理和诊断方式。影像人工智能产品的生产研发、验证确认、临床质控等活动均需要依托图像数据的高质量标注结果。为了规范CCTA的数据标注,本共识对CCTA的标注提出统一的共识和指导意见,从数据集适用范围和定义、数据要求、标注流程及标注规则等各个方面阐述专家组就该问题达成的共识,旨在帮助业内形成统一认知,提高CCTA数据的标注质量,推动相关数据集的建设,促进CCTA相关的人工智能研究规范化发展,提升冠状动脉疾病的诊疗水平。

关键词：体层摄影术,X线计算机冠状动脉CT血管成像数据标注质量控制

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A Case Study on the Working Efficacy of Stakeholders at Improving Occupational health Conditions in Small-scale Enterprises

A Case Study on the Working Efficacy of Stakeholders at Impr...

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2010 the 5th International Academic Conference on Environmental and Occupational Medicine

作者： GUO Wei-jun,ZHU Mei-fen,HE Yong-hua 1.center of disease Control and Prevention,Shengzhou County 312400,Zhejiang Province 2.Agency for Public health Supervision,Nanhui District 201300,shanghai 3.School of Public health/WHO Collaborating center for Occupational health,Fudan University,shanghai 200032

＜正＞[Objective]This study investigates the current working efficacy of local governmental health supervisors, employers,employees,and occupational health service agents in improving work conditions of small-scale manufacturing enterprises.[Methods]Six factories of small-scale lead-acid battery manufacturers with about 900 total workers were recruited. The working efficacy of each stakeholder was assessed by comparing the airborne lead level in workplaces and blood-lead concentration among exposed workers in 2004 and 2007.[Results]The engaged stakeholders took measures to protect workers. Nevertheless,there were 50%and 22%worksites with airborne lead concentration above the national standards in 2004 and 2007,*** workers had blood-lead level above the national biological exposure limit.[Conclusion]The data reveal that the working efficacy was not sufficient in most of the *** collaboration,strengthening inspection and measure implementation,and competent multidisciplinary professional teams remain imperative.

关键词： occupational health stakeholders efficiency small-scaled enterprises lead battery manufacturing

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Status of Schistosomiasis in Domestic Animals in the People's Republic of china from 2005 to 2010

Status of Schistosomiasis in Domestic Animals in the People'...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一...

作者： Liu Jinming~(1*),Song Junxia~2,Jin Yamei~1,Ma Shichun~3,Zhu Chunxia~4,He Liang~5,Zhou Yi~6,Liu Yipin~7,Yang Aiguo~8,Dong Guodong~9,Reng Xuefeng~(10),Chi Lijuan~3,Shi Yaojun~1,Li Hao~1,Lin Jiao-jiao~1 (1 national laboratory of Animal Schistosomiasis Control/Key laboratory of Animal Parasitology, Ministry of Agriculture,shanghai Veterinary Research Institute of chinese Academy of Agricultural Science,shanghai,P.R.china 2 china Veterinary Bureau,Ministry of Agriculture,Beijing,P.R.china 3 china Animal disease Control center,Beijing,P.R.china 4Hunan Animal disease Control center, Changsha,P.R.china 5 Jiangxi domestic animal schistosomiasis control station,Nanchang,P.R.china 6 Hubei Animal disease Control center,Wuhan,P.R.china 7 Anhui Animal disease Control center, Hefei,P.R.china 8 Sichuan Animal disease Control center,Chengdu,P.R.china 9 Yunnan Animal disease Control center,Kunming,P.R.china 10 Jiangsu Animal disease Control center,Nanjing 210036,P.R.china)

This paper reports the kinetics of schistosomiasis in domestic animals in china from 2005 to *** these years,the prevalence of Schistosma japonicum(***) infection decreased significantly from 3.60%to 1.08%,and the estimated number of infected domestic animals reduced from 72223 to *** all estimated 208421 infected domestic animals,80.04%(166820) cases were in bovine species(cattle and buffalos) and 76.2%(158812) were situated in lake-marshland-type endemic regions including Hunan,Hubei,Jiangxi and Anhui *** worst affected were Dongting(Hunan province) and Poyang(Jiangxi province) lake regions,where 70.14%of the infected animals detected in 2010 lived in. The results suggest that the prevalence of domestic animal schistosomiasis has been reduced significantly. The study has reinforced the idea that bovine animals are the main infectious source for transmission of schistosomiasis,and that lake-marshland-type endemic regions,especially the Poyang and Dongting lake regions,are key regions in which to implement control of schistosomiasis.

关键词： Schistosomiasis Domestic animal Endemic situation china

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中国大陆不同地区光壳钉螺遗传多样性的RAPD分析

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中国血吸虫病防治杂志 2003年第4期15卷 251-254页

作者：许静郑江中国疾病预防控制中心寄生虫病预防控制所 WHO疟疾血吸虫病和丝虫病合作中心上海200025

目的用随机扩增多态性技术(RAPD)对中国大陆5省不同地区的光壳钉螺进行分析,研究光壳钉螺群间的遗传多样性。方法提取钉螺头足部的基因组DNA,用随机引物进行扩增,扩增产物经8％的聚丙烯酰胺凝胶电泳,0.6％硝酸银染色,判别结果进行分... 详细信息

目的用随机扩增多态性技术(RAPD)对中国大陆5省不同地区的光壳钉螺进行分析,研究光壳钉螺群间的遗传多样性。方法提取钉螺头足部的基因组DNA,用随机引物进行扩增,扩增产物经8％的聚丙烯酰胺凝胶电泳,0.6％硝酸银染色,判别结果进行分析。结果 9个螺群的平均遗传相似性为0.7214,平均百分匹配数为0.6809,聚类结果表明可分为3个分类群,云南大理和四川普格的钉螺为第一分类群,福建福清和江苏东台的钉螺为第二分类群,安徽的4个螺群和江苏宜兴的钉螺为第三分类群。结论在我国各地的光壳钉螺螺群间存在一定的亲缘关系的同时,已发生了较大的遗传变异,聚类结果中螺群的聚类地位和其地理分布基本上一致,但江苏东台光壳螺群的聚类地位有待进一步分析和研究。

关键词：湖北钉螺随机扩增多态性DNA 基因组 DNA

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genome-Wide Transcriptional Analysis of temperature shift of L. interrogans serovar lai Strain 56601

Genome-Wide Transcriptional Analysis of temperature shift of...

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首届全国微生物基因组学学术研讨会

作者： Jin-Hong QIN, Yue-Ying SHENG, Zhi-Ming ZHANG, Ping HE, Bao-Yu HU, Yang YANG, Shi-Gui LIU, Guo-Ping Zhao, Xiao-Kui GUO School of Life Science /School of Chemical Engineering, Sichuan University, Chengdu 610041, PR china Department of Microbiology and Parasitology, shanghai Jiao Tong University School of Medicine, shanghai 200025, PR china national Engineering center for Biochip at shanghai, 151 libing Road, Zhangjiang Hi-Tech Park, Pudong, shanghai 201203, china.

Leptospira interrogans is an important mammalian pathogen. Transmission from an environmental source requires adaptations to a range of new environmental conditions in the organs and tissues of the infected host Since many pathogenic ’ bacteria utilize temperature to discern their environment and regulate the synthesis of appropriate proteins, we investigated the effects of temperature on protein synthesis in L. interrogans. We used the L. interrogans serovar lai Strain 56601 genome to construct the microarray. DNA microarray analysis was used to investigate the influence of temperature on global gene expression in strain 56601 grown to mid-exponential phase at 28癈 and 37癈. A total of 112 genes expression varied significantly in expression at 37癈 relative to 28癈. The differentially expressed genes included ten kinds of genes which included membrane proteins, hemolysins encoded genes, heat shock proteins, lipoproteins, two-component system, chemotaxis and flagellar proteins, transcriptional regulators, transport and binding protein, metabolic related protein and proteins with no known homologue. Real-time reverse transcription-PCR assays confirmed the microarray data. The data delineate the spectrum of temperature-regulated gene expression in an important human pathogen and provide many unforeseen results of pathogenesis investigation.

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Surveillance of Schistosoma japonicum Infection in Domestic Ruminants in the Dongting Lake region,Hunan Province,china

Surveillance of Schistosoma japonicum Infection in Domestic ...

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中国畜牧兽医学会兽医公共卫生学分会第三次学术研讨会

作者： Jinming Liu1,Chunxia Zhu2,Yaojun Shi1,Hao Li1,Lanpin Wang2,Shangtian Qin2,Saie Kang3,Yanpin Huang4,Yamei Jin1 & Jiaojiao Lin 1(1.national laboratory of Animal Schistosomiasis Control/Key laboratory of Animal Parasitology,Ministry of Agriculture,shanghai Veterinary Research Institute,chinese Academy of Agricultural Sciences,shanghai,PR china 2.Hunan Animal disease Control center,Changsha,PR china 3.Yueyang County Animal Husbandry Bureau,Yueyang,PR china 4.Yuanjiang Municipal Animal Husbandry and Fishery Bureau,Yuanjiang,PR china)

Background:Schistosomiasis japonica is prevalent in Asian countries and it remains a major public health problem in *** major endemic foci are the marsh and lake regions of southern china,particularly the Dongting Lake region bordering Hunan and Hubei provinces,and the Poyang Lake region in Jiangxi *** ruminants,especially bovines,have long been considered to play a major role in the transmission of Schistosoma japonicum to *** and Findings:A miracidial hatching technique was used to investigate the prevalence of *** infections in domestic ruminants and field feces collected from two towns located to the south and east of Dongting Lake,Hunan province,between 2005 and *** overall prevalence of infection was not significantly reduced from 4.93% in 2005 to 3.64% in 2008,after which it was maintained at this *** comprised 23.5-58.2% of the total infected ruminants,while goats comprised 41.8-76.5%.Infection rates in cattle and goats were significantly higher than those found in buffalo in most study *** prevalence in buffalo younger than three years was significantly higher than that in those aged over three *** the positive field samples of feces were derived from bovines in *** Matang Town,61.22% of the positive field feces were from bovines,while the rest were from *** positive rates for field feces were approximately the same in April and November/***:The present study found that bovines and goats are major sources of *** infection in the Dongting lake region and there was age-related resistance in *** bovines and goats should be treated equally when controlling *** infections in the Dongting lake *** is essential to conduct an additional mass treatment in late March or early April,in addition to the original treatment scheme.

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Pestalotiopsis fici genome reveals the richness of secondary metabolites of endophytic fungus

Pestalotiopsis fici genome reveals the richness of secondary...

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2012年中国菌物学会学术年会

作者： Xiuna Wang~(1 2) Ling Liu~1 Xinyu Zhang~1 Gang Liu~1 Liangdong Guo~1 Yongsheng Che~1 Chengshu Wang~3 Zhiqiang An~4 Liyun Guo~2 Xingzhong Liu~(1*) (1 State Key laboratory of Mycology Institute of Microbiology chinese Academy of Sciences No 3 1~(st) West Beichen Rd Chaoyang District Beijing 100101 china 2 Department of Plant Pathology china Agricultural University Beijing 100094 china 3 Key laboratory of Insect Developmental and Evolutionary Biology Institute of Plant physiology and Ecology shanghai Institutes for Biological Sciences chinese Academy of Sciences shanghai 200032 china 4 Institute of Molecular Medicine University of Texas health Science center at Houston Houston TX 77030 USA) Department of Plant Pathology China Agricultural University Beijing 100094 China State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of SciencesNo 3 1st West Beichen Rd Chaoyang District Beijing 100101 China Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Institute of Molecular Medicine University of Texas Health Science Center at Houston HoustonTX 77030 USA Department of Plant Pathology China Agricultural University Beijing 100094 China State Key laboratory of Mycology Institute of Microbiology Chinese Academy of SciencesNo 3 1st West Beichen Rd Chaoyang District Beijing 100101 China

＜正＞Endophytic fungi grow within their plant hosts without causing apparent disease symptoms and are an important source of novel bioactive secondary metabolites（Schulz,*** C. Boyle 2006,Strobel,G.,***,et al.2004）.Pestalotiopsis fici,an important woody plant endophyte,produces numerous bioactive secondary metabolites（Liu,L 2011）.To reveal the mystery of so many secondary metabolites,the genome of *** was shotgun sequenced using a Roche 454 GS FLX *** is a 51.91 Mb genome size containing～16,063 predicted protein-encoding *** indicated that the gene clusters for secondary metabolites were significantly expanded in number and diversity comparing with other fungal *** protein families related to secondary metabolism,such as MFS general substrate transporter, aromatic compound dioxygenase,cytochrome P450,FAD-binding domain oxidase,ankyrin repeat, Zn2/Cys6 DNA-binding domain *** more abundant in the genome of *** based on the Pfam and CAFE *** preliminary analysis also found evidence for horizontal transfer of a secondary metabolite gene cluster from host plants to *** research explained that the richness of secondary metabolites in endophytic fungi from gene expansion and horizontal transfer of gene clusters.

关键词： Endophytic fungi secondary metabolite genome

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红丹爽身粉致儿童铅中毒的调查

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实用儿科临床杂志 2010年第6期25卷 416-418页

作者：林国桢颜崇淮李科彭荣飞刘翔翊杜琳广州市疾病预防控制中心慢性非传染性疾病预防控制科广州510080 上海交通大学医学院附属新华医院上海市环境与儿童健康重点实验室上海200092 广州市疾病预防控制中心理化检验科广州510080

目的对一兄妹铅中毒进行调查,查明其中毒的原因。方法在铅中毒患儿肘部静脉处抽取静脉血2mL,分别置于用0.2mol·L-1EDTA抗凝的试管内,用塞曼石墨炉原子吸收分光光谱仪测定血铅水平。铅中毒诊断标准依据卫生部2006年颁布的《儿童高铅血... 详细信息

目的对一兄妹铅中毒进行调查,查明其中毒的原因。方法在铅中毒患儿肘部静脉处抽取静脉血2mL,分别置于用0.2mol·L-1EDTA抗凝的试管内,用塞曼石墨炉原子吸收分光光谱仪测定血铅水平。铅中毒诊断标准依据卫生部2006年颁布的《儿童高铅血症和铅中毒分级和处理原则(试行)》。用自行设计的调查问卷对患儿进行铅暴露调查。用Innov-α4000便携式X线荧光分析仪(XRF)对其家庭中物品进行铅水平检测。抽取相关的爽身粉,每个样品加入浓硝酸,并经微波消化,用火焰原子吸收光谱仪(FAAS)检测爽身粉中的铅水平。结果2例患儿入院血铅水平分别为303g.L-1和385g.L-1,诊断为中度铅中毒。给予依地酸二钠钙静脉滴注驱铅,5d后血铅分别降至118g.L-1和88g.L-1。应用XRF对其家庭用品进行铅水平检测,食品、水、儿童玩具和家具未超标,但一种供2例患儿使用的散装爽身粉铅水平>100000mg·kg-1,经FAAS测得铅水平为214000mg·kg-1,超过国家化妆品卫生标准(40mg·kg-1)5000多倍,超过食品卫生标准(5mg·kg-1)40000多倍。经询问这种爽身粉来自江西省赣州农村,由中药红丹与滑石粉混合而成,抽查赣州市药店出售的红丹粉16份,铅水平平均为817375mg·kg-1。抽查红丹爽身粉13份,铅水平全部超标,平均为206784mg·kg-1,普通盒装爽身粉6种,铅水平均为5mg·kg-1。结论2例患儿铅中毒是由于使用红丹爽身粉引起,红丹爽身粉在我国南方农村地区普遍使用,应引起重视。

关键词：铅中毒中药儿童

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原发纵隔大B细胞淋巴瘤诊断与治疗中国专家共识(2024年版)

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中华血液学杂志 2024年第3期45卷 209-214页

作者：中华医学会血液学分会淋巴细胞疾病学组中华医学会血液学分会淋巴细胞疾病学组中国临床肿瘤学会(CSCO)淋巴瘤专家委员会徐兵赵维莅马军赵海军易红梅查洁不详厦门大学附属第一医院厦门大学血液病学系厦门市血液病质控中心厦门市血液治疗精准诊治重点实验室厦门361003 上海血液学研究所医学基因组学国家重点实验室转化医学国家重大科技基础设施(上海)上海交通大学医学院附属瑞金医院上海200025 哈尔滨血液病肿瘤研究所哈尔滨150010 厦门大学附属第一医院上海交通大学医学院附属瑞金医院

原发纵隔大B细胞淋巴瘤(primary mediastinal large B-cell lymphoma,PMBL)是一种起源于纵隔的成熟侵袭性大B细胞淋巴瘤,具有独特的临床、病理及分子学特征。近年来,对PMBL发病机制的认识及治疗均有不同程度的更新,特别是新药治疗领域... 详细信息

原发纵隔大B细胞淋巴瘤(primary mediastinal large B-cell lymphoma,PMBL)是一种起源于纵隔的成熟侵袭性大B细胞淋巴瘤,具有独特的临床、病理及分子学特征。近年来,对PMBL发病机制的认识及治疗均有不同程度的更新,特别是新药治疗领域取得了较大进展。为提高我国临床医师对PMBL的诊断及治疗水平,中华医学会血液学分会淋巴细胞疾病学组和中国临床肿瘤学会(CSCO)淋巴瘤专家委员会组织相关专家组,制订了本共识。

关键词：淋巴瘤,大B细胞纵隔诊断治疗

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