Detection and identification for nematode Gnathostoma in imported Monopterus albus

作     者：Li Shuqing~1,Li Wenwen~2,Chen Zhifei~1,Li Jian~2,Chen Shaohong~3,Zhang Yongnian~3 Huang Weiyi~2 Wang Qiaoquan~1 1.Shanghai Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China,Shanghai 200135 2.College of Animal Science and Technology,Guangxi University,Nanning 530005 3.Chinese Center for Disease Control and Prevention,National Institute of Parasitic Diseases, Shanghai 200025 

会议名称：《中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会》

会议日期：2011年

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

摘      要：Objective To inspect the third stage larvae of Gnathostoma in imported Monopterus albus,and identify its species..Method 10 batches of imported Monopterus albus to Shanghai from abroad were detected for nematode Gnathostoma from January 2010 to March *** 52 Monopterus albus,3～10/batch,From Philippines(25),Indonesia(24) and Bangladesh(3),dissect each Monopterus albus,then minced,protease digestion,the suspension was filtered with 10 mesh copper screen,at the last take the precipitation of the *** the complete parasites under the stereoscope,morphological identification,and calculate the infection rate and average *** extracted genomic DNA of Gnathostoma,amplified internal transcribed spacer region 2 and cytochrome c oxidase subunit 1 by PCR,then Gel electrophoresis and *** sequences were aligned with corresponding sequences in *** The third stage larvas of nematode Gnathostoma were detected in Monopterus albus from Indonesia and Philippines,infection rates were 36.0%(9/25) and 50.0% (12/24),average infectiosity were 7.77(70/9) and 2.83(34/12).There wasn’t Gnathostoma in imported Monopterus albus from *** the microscope,the larvas have one cephalic bulb,4 rings hooklets on the cephalic bulb,the body surface with cross striations and small spines,the front of the body spines were obviously and density,the rear spines getting small and *** had 1 cervical papillae,4 cervical *** characteristics was similar to Gnathostoma *** results showed,ITS-2 and cox1 PCR products were 647 bp and 441 bp,with the expected *** alignment analysis showed that,two PCR products had 99%～100%consistency with Gnathostoma spinigerum ITS-2(GenBank accession number AB181155 and Z97175) and coxl (GenBank accession number AY501388,AB180099 and AB551552).Conclusion All of the Gnathostoma detected in imported Monopterus albus from Philippines and Indonesia were Gnathostoma spinigerum.