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A20 overexpression under control of mouse osteocalcin promoter in MC3T3-E1 cells inhibited tumor necrosis factor-alpha-induced apoptosis

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Acta Pharmacologica Sinica 2006年第9期27卷 1231-1237页

作者： Yue-juan QIN~2 Zhen-lin ZHANG~(2,4.Lu-yang YU~3 Jin-wei HE~2 Ya-nan HOU~3 Tian-jin LIU~3 Jia-cai WU~3 Song-hua WU~2 Li-he GUO~3 2 center for Preventing and Treating Osteoporosis,Osteoporosis Research Unit,shanghai Sixth People's Hospital,shanghai Jiao Tong University,shanghai 200233,{4. 3 Institute of Biochemistry and Cell Biology,shanghai institutes for biological Science,chinese academy of sciences,shanghai 2.0031,{4. centerforPreventingandTreatingOsteoporosis OsteoporosisResearchUnitShanghaiSixthPeople'sHospitalShanghaiJiaoTongUniversityShanghai200233China InstituteofBiochemistryandCellBiology ShanghaiInstitutesforBiological

Aim:To construct an A20 expression vector under the control of mouse osteocalcinpromoter(OC-A20),and investigate osteoblastic MC3T3-EI cell line,whichstably overexpresses A20 protein prevented tumor necrosis factor(TNF)-alpha-induced ***:OC-A20 vector was constructed by fusing a frag-ment of the mouse osteocalcin gene-2 promoter with human A20 *** the mouse MC3T3-E1 cell line,stably transfected by A20,*** expression of A20 mRNA and A20 protein in the cells weredetected by reverse transcription-polymerase chain reaction(RT-PCR)and West-ern blot analysis,*** determine the specificity of A20 expression inosteoblast,the mouse osteoblastic MC3T3-E1 cell line and mouse embryo fibro-blast NIH3T3 cell line were transiently transfected with *** anti-apoptoticrole of A20 in MC3T3-E1 cells was determined by Flow cytometric analysis(FACS),terminal dUTP nick endo-labeling(TUNEL)and DNA gel electrophoresis analysis(DNA Ladder),***:Weak A20 expression was found in MC3T3-E1 cells with the primers of mouse A20.A20 mRNA and A20 protein expressionwere identified in MC3T3-E1 cells transfected with OC-A20 using RT-PCR andWestern blot *** A20 mRNA expression was found in MC3T3-E1 cellafter MC3T3-E1 cells and NII-/3T3 cells were transient transfected with *** obviously occurred in the rate of apoptosis in the OC-A20 group com-pared with the empty vector(pcDNA3)group by FACS(P<0.001).A significantincrease in TUNEL positive staining was found in the pcDNA group comparedwith OC-A20 group(P<0.001).Simultaneously,similar effects were demonstratedin DNA gel electrophoresis ***:We constructed an osteoblast-specific expression vector that expressed A20 protein in MC3T3-E1 cells andconfirmed that A20 protects osteoblast against TNF-alpha-induced apoptosis.

关键词： A20 osteoblast apoptosis tumor necrosis factor-alpha

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Cytoplasmic auxin signaling

Cytoplasmic auxin signaling

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从植物科学到农业发展——2012全国植物生物学大会

作者： Zhenbiao Yang Department of Botany and Plant sciences and center for Plant Cell Biology,University of California at Riverside,CA 92521,USA shanghai Institute of Plant Physiology and Ecology,shanghai {4. of biological sciences,chinese academy of sciences,shanghai,china.

Auxin regulates the formation of various developmental and morphogenetic patterns in plants,but the molecular and cellular mechanisms for the auxin actions are not well *** investigate these mechanisms using Arabidopsis leaf epidermal pavement cells(PCs) as a model system,which form the jigsaw puzzle cell pattern with interdigitated lobes and *** have shown that pavement cell interdigitation is controlled by localized ROP GTPase signaling,which impinges on the organization of cortical actin microfilaments and microtubules to generate interdigitating lobes and *** recent studies indicate that auxin is a signal that activates the generation of multi-polarity to form lobes in pavement cells and coordinates lobe formation with indentation formation by activating ROP GTPase signaling through an Auxin-Binding Protein 1(ABP1) dependent auxin perception *** cytoplasmic auxin signaling system,which is distinct from the well-established TIR1-dependent nuclear auxin signaling system that regulates gene transcription,modulates local coordination of lobe and indentation formation within a PC and between adjacent PCs through its modulation of PIN protein *** ROP-based cytoplasmic auxin signaling also regulates the subcellular distribution of PINs in root cells,supporting a widespread role for this new auxin signaling system in plants 4. Recent findings of additional components in this new auxin signaling system will be discussed.

关键词： AUXIN SIGNALING ABP1 PIN1 PIN2

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Effect of GNTI,a kappa opioid receptor antagonist,on MK-801-induced hyperlocomotion and stereotypy in mice

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Acta Pharmacologica Sinica 2006年第11期27卷 1401-1408页

作者： Chun-ting QI~2 Hong ZOU~2 Chen-hao ZHANG~2 Qing-lian XIE~2 Mei-lei JIN~(2,4.Lei YU~(3,4.2 Research center for Biotechnology,shanghai institutes for biological {4.,chinese academy of {4.,shanghai 200233,china 3 Department of Genetics and center of Alcohol Studies,Rutgers,the State University of New Jersey 08854.8001,USA ResearchcenterforBiotechnology ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200233China DepartmentofGeneticsandcenterofAlcoholStudies RutgerstheStateUniversityofNewJersey08854-8001USA

Aim:To examine the effect of GNTI [5′-guanidinyl-17-(cyclopropylmethyl)-6,7-dehydro-4.5α-epoxy-3,14.dihydroxy-6,7-2′,3′-indolomorphinan],a selective antago-nist for the kappa opioid receptor,in the MK-801(dizocilpine maleate)-inducedbehavioral model of psychosis in schizophrenia as a way to explore the involve-ment of the kappa opioid receptor in modulating psychotic symptoms ***:Two doses of MK-801(0.3 mg/kg and 0.6 mg/kg)wereadministered by systemic injection in mice to induce psychosis-like behavior as arodent schizophrenia model,preceded by an injection of different doses of *** locomotion and stereotypy were measured as the behavioral endpoints forquantitative ***:GNTI inhibited MK-801-induced hyperlocomotionand *** particular,GNTI showed differential modulation of stereotypyinduced by 0.3 mg/kg vs 0.6 mg/kg ***:Antagonism of kappaopioid receptors attenuates MK-801-induced behavior,suggesting a potentialinvolvement of the kappa opioid receptor in psychosis-like symptoms *** appears to be a useful pharmacological tool to explore thekappa opioid receptor function in vivo.

关键词： GNTI kappa opioid receptor MK-801 schizophrenia model

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Construction of a small peptide library related to inhibitor OM99-2 and its structure-activity relationship to β-secretase

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Acta Pharmacologica Sinica 2006年第12期27卷 1586-1593页

作者： Bin HU Bing XIONG Bei-ying QIU Xin LI Hai-ping YU Kun XIAO Xin WANG Jia LI Jing-kang SHEN~2 State Key Laboratory of Drug Research and National center for Drug Screening,shanghai Institute of Materia Medica,shanghai institutes for {4. sciences,chinese academy of sciences,shanghai 201203,china State Key Laboratory of Drug Research and National center for Drug Screening Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China

Aim:To develop probes for detecting the binding specificity between β-secretaseand substrate,and provide reliable biological activity data for further researchingencircling substrate-based ***:To prepare the inhibitors,thehydroxyethylene(HE)segment including P1and P1′ was synthesized after multi-step reactions;the combination of all segments was then completed through solidphase *** human β-secretase ectodomain(amino acid resi-dues 1-4.0)was expressed as a secreted protein with a C-terminal His tag in insectcells using baculovirus infection,and all compounds were evaluated in thisβ-secretase enzyme *** order to understand the interaction in detail,the theoretical methods,namely molecular dynamics(MD)simulation and mo-lecular mechanics-generalized-born surface area(MM-GBSA)analysis,were per-formed on the complex of β-secretase and OM99-2 to obtain the geometrical andenergetical ***:We designed and constructed a positional scan-ning combinatorial library including 16 compounds;all members of the librarywere synthesized based on HE dipeptide ***-activity relationshipstudies at the P4.P1and P1′-P4. positions led to the discoveries of P and P’sidesbinding specificity and potent inhibitors 14.18,and *** binding free energyon the whole system and every residue were compared to the biological ***:The removal of P4. yielded inhibitor 22(A3*B2)with highpotency;further truncation of P3′ gave inhibitor 18(A3*B1)with equal activity,implying that the right side of the inhibitors play a less important role and could beeasily simplified,while change on the P side may cause substantial results.

关键词： β-secretase hydroxyethylene OM99-2 Alzheimer’s disease structure-activity relationship

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Characterization of Structural Genes Involved in Anthocyanin Biosynthesis in Sweet Potato(Ipomoea batatas Lam.)

Characterization of Structural Genes Involved in Anthocyanin...

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2011全国植物生物学研讨会

作者： Hongxia Wang~a,Peng Zhang~(a,b) a Institute of Plant Physiology & Ecology,shanghai institutes for biological sciences,{4. academy of sciences,300 Fenglin Road,shanghai 200032,china b Chenshan Plant Science Research center,{4. academy of sciences,Chenshan Botanical Garden,3888 Chenhua Road,shanghai 201602,china

Purple sweet potato accumulates a high amount of anthocyanins,which are mainly composed of non-,mono-,and diacylated gluocosides of cyanidin and *** the function of anthocyanins from purple sweet potato has been well studied in food and pharmaceutical products,the biosynthesis of anthocyanins in the crop is still largely *** this study,four structural genes involved in anthocyanin biosynthesis,flavanone 3-hydroxyrase(F3H), dihydroflavonol 4.reductase(DFR),anthocyanidin synthase(ANS),and glucosyltransferase(GT),were cloned from the purple cultivar Ayamurasaki and intensively ***-expression of the full-length cDNAs of DFR,F3H,ANS

关键词： sweet potato anthocyanin biosynthesis expression regulation flavonoids

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Molecular dynamics simulations of interaction between protein-tyrosine phosphatase 1B and a bidentate inhibitor

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Acta Pharmacologica Sinica 2006年第1期27卷 100-110页

作者： Gui-xia LIU~(2,3,4. Jin-zhi TAN~2 Chun-ying NIU~2 Jian-hua SHEN~(2,3) Xiao-min LUO~2 Xu SHEN~(2,3) Kai-xian CHEN~2 Hua-liang JIANG~(2,3,4.2 center for Drug Discovery and Design,State Key Laboratory of Drug Research,shanghai Institute of Materia Medica,shanghai institutes for {4. sciences,chinese academy of sciences,shanghai 201203,china 3 School of Pharmacy,East china University of Science and Technology shanghai 200237,china centerforDrugDiscoveryandDesign StateKeyLaboratoryofDrugResearchShanghaiInstituteofMateriaMedicaShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai201203China

Aim:To investigate the dynamic properties of protein-tyrosine phosphatase (PTP)1B and reveal the structural factors responsible for the high inhibitory potencyand selectivity of the inhibitor SNA for ***:We performed moleculardynamics (MD) simulations using a long time-scale for both PTP1B and PTP1Bcomplexed with the inhibitor SNA,the most potent and selective PTP1B inhibitorreported to *** trajectories were analyzed by using principal ***:Trajectory analyses showed that upon binding the ligand,theflexibility of the entire PTP1B molecule *** most notable change is themovement of the *** simulation results also indicated that electro-static interactions contribute more to PTP 1B-SNA complex conformation than thevan der Waals interactions,and that Lys4.,Arg4.,and Asp4. play important rolesin determining the conformation of the inhibitor SNA and in the potency andselectivity of the *** these,Arg4. contributed *** results werein agreement with previous experimental ***:The informationpresented here suggests that potent and selective PTP1B inhibitors can be de-signed by targeting the surface residues,for example the region containing Lys4.,Arg4.,and Asp4.,instead of the second phosphate binding site (besides theactive phosphate binding site).

关键词： molecular dynamics simulation principal component analysis protein-tyrosine phosphatase 1B type 2 diabetes

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Refined Phylogenetic Profiles Method for Predicting Proteinprotein Interaction

Refined Phylogenetic Profiles Method for Predicting Proteinp...

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中国蛋白质组学第二届学术大会

作者： Jingchun Sun, Zhen Liu, Qi Liu, Jinlin Xu, Tieliu Shi, Yixue Li (School of Life Science & Technology, shanghai Jiaotong University, shanghai, 20024., china Departments of Biology, Hunan Normal University, Changsha, 4.0081, china {4. center, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 2.0031, china)

Many aspects of cellular biology involve protein-protein interactions and comprehensively identifying them is important to systematically understand the biological mechanisms such as molecular mechanisms of metabolic or signal pathway and the construction of complexes. The availability of increasing completely sequenced genomes makes the in silico or experiment based reverse proteomics methods to detect protein-protein interaction popular, such as widely used yeast two-hybrid test, gene neighbor method, gene fusion method and the phyloge netic profiles method and so on.

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Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis

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Cell Research 2007年第5期17卷 422-434页

作者： Jin-Ying Gou1,2,3 Ling-Jian Wang1,2 Shuang-Ping Chen4.Wen-Li Hu1,2 Xiao-Ya Chen1,2 1 National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, shanghai institutes for biological sciences,{4., 300 Fenglin Road, shanghai 200032, china 2 CAS center of shanghai Chenshan Botanical Garden, 300 Fenglin Road, shanghai 200032, china 3 Graduate School of{4., shanghai 200032, china 4.Department of Computer Science and Technology, University of Science and Technology of china, Hefei 230026, china NationalKeyLaboratoryofPlantMolecularGenetics InstituteofPlantPhysiologyandEcologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciences300FenglinRoadShanghai200032China

Cotton fibers elongate rapidly after initiation of elongation, eventually leading to the deposit of a large amount ofcellulose. To reveal features of cotton fiber cells at the fast elongation and the secondary cell wall synthesis stages, wecompared the respective transcriptomes and metabolite profiles. Comparative analysis of transcriptomes by cDNA arrayidentified 633 genes that were differentially regulated during fiber development. Principal component analysis (PCA)using expressed genes as variables divided fiber samples into four groups, which are diagnostic of developmental *** grouping results are also found if we use non-polar or polar metabolites as variables for PCA of developingfibers. Auxin signaling, wall-loosening and lipid metabolism are highly active during fiber elongation, whereas cellulosebiosynthesis is predominant and many other metabolic pathways are downregulated at the secondary cell wall synthesisstage. Transcript and metabolite profiles and enzyme activities are consistent in demonstrating a specialization process ofcotton fiber development toward cellulose synthesis. These data demonstrate that cotton fiber cell at a certain stage has itsown unique feature, and developmental stages of cotton fiber cells can be distinguished by their transcript and metaboliteprofiles. During the secondary cell wall synthesis stage, metabolic pathways are streamed into cellulose synthesis.

关键词： cotton fiber transcriptome metabolite profile auxin cell elongation cellulose synthesis

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QTL Mapping for 1,000- Grain Weight in RILs of Rice

QTL Mapping for 1,000- Grain Weight in RILs of Rice

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第十二届全国植物基因组学大会

作者： Junping Shi,Lu Wang,Bin Han~*,Ahong Wang National center For Gene Research and Institute of Plant Physiology and Ecology,shanghai institutes of biological {4., chinese academy of sciences,shanghai 200233,china

Rice(Orvza sativa L.) is one of human’s major food staples,and 1,000-grain weight(TGW) which is determined by grain length,width,thickness and filling rate is a vital component of grain *** identify the genes conditioning this trait and reveal the molecular mechanism of them,we carried out a quantitative trait locus(QTL) mapping with 150 rice recombinant inbred lines(RILs) which were developed from a cross between Nipponbare(japonica,low TGW) and 93-11(indica,high TGW).4.QTLs were detected;two localized on chromosome 5,the other two localized on chromosome 8 and 10,*** we focused on a ma-

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香樟树种子中两种Ⅱ型核糖体失活蛋白凝集素活性的研究

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生物化学与生物物理进展 2004年第7期31卷 650-654页

作者：杨海燕李荣秀刘望夷中国科学院上海生命科学研究院生物化学与细胞生物学研究所中国科学院上海生命科学研究院生物工程研究中心

从香樟树成熟的种子分离到两种新的Ⅱ型核糖体失活蛋白 :新丰毒蛋白和辛纳毒蛋白 .两者A链的分子质量虽然相差近一倍 ,但B链的分子质量相同 .Ⅱ型核糖体失活蛋白的A链是RNAN 糖苷酶 ,B链是凝集素 ,A链进入细胞表现毒性在很大程度上依赖... 详细信息

从香樟树成熟的种子分离到两种新的Ⅱ型核糖体失活蛋白 :新丰毒蛋白和辛纳毒蛋白 .两者A链的分子质量虽然相差近一倍 ,但B链的分子质量相同 .Ⅱ型核糖体失活蛋白的A链是RNAN 糖苷酶 ,B链是凝集素 ,A链进入细胞表现毒性在很大程度上依赖于B链的糖结合活性和特异性 .对辛纳毒蛋白和新丰毒蛋白B链的凝集素活性进行了测定和比较 .红细胞凝集实验显示辛纳毒蛋白和新丰毒蛋白具有相同的细胞凝集活性 ,半抗原抑制实验发现它们都属于半乳糖型核糖体失活蛋白 ,荧光光谱法显示它们的糖结合常数也相同 .

关键词：新丰毒蛋白辛纳毒蛋白凝集素荧光光谱法

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