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A meta-analysis of oxidative stress markers in schizophrenia

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中国科学：生命科学英文版 2010年第1期53卷 112-124页

作者： ZHANG Ming1,2,ZHAO ZhongMing3,HE Lin1,2,4.& WAN ChunLing1,2 1 Bio-X center,Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education),Shanghai Jiao Tong university,Shanghai 200030,china 2 Institutes for Nutritional Sciences,Shanghai Institute of biological Sciences,Chinese Academy of Sciences,Shanghai 200031,china 3 Departments of Biomedical Informatics,Psychiatry,and Cancer Biology,Vanderbilt university Medical center,Nashville,TN 37232,USA 4.Institutes of Biomedical Sciences,Fudan university,Shanghai 200032,china 1. Bio-X center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 China2. Institutes for Nutritional Sciences Shanghai Institute of Biological Sciences Chinese Academy of Sciences Shanghai 200031 China3. Departments of Biomedical Informatics Psychiatry and Cancer Biology Vanderbilt University Medical Center Nashville TN 37232 USA4. Institutes of Biomedical Sciences Fudan University Shanghai 200032 China

Oxidative stress has been identified as a possible element in the neuropathological processes of schizophrenia(SCZ).Alteration of oxidative stress markers has been reported in SCZ studies,but with inconsistent *** evaluate the risk of oxidative stress to schizophrenia,a meta-analysis was conducted,including five markers of oxidative stress [thiobarbituric reactive substances(TBARS),nitric oxide(NO),catalase(CAT),glutathione peroxidase(GP) and superoxide dismutase(SOD)] in SCZ patients versus healthy *** study showed that TBARS and NO significantly increased in SCZ,while SOD activity significantly decreased in the disorganized type of SCZ *** significant effect size was found for the activities of GP and CAT in SCZ patients(P>0.05).Egger’s regression test observed no significant publication bias across the oxidative stress markers,but found high heterogeneities in all the 5 *** subgroup analysis suggested that the ethnicity,sample size of patients and sample sources may contribute to the heterogeneity of the results for TBARS,NO and *** result further demonstrated the involvement of oxidative stress in the pathophysiology of schizophrenia.

关键词： schizophrenia oxidative stress meta-analysis lipid peroxidation nitric oxide antioxidant enzymes

Identifying Qingkailing(清开灵)ingredients-dependent mesenchymal-epithelial transition factor-axiation“π”structuring module with angiogenesis and neurogenesis effects

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Journal of Traditional Chinese Medicine 2024年第1期44卷 35-43页

作者： CHENG kunming YUAN Jianan LIU Jun ZHANG Shengpeng XU Qixiang XIE Yong ZHAO Jingfeng ZHANG Xiaoxu TANG Xudong ZHENG Yongqiu WANG Zhong Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicines in Southern Anhui Teaching and Research Section of Traditional Chinese MedicineSchool of PharmacyWannan Medical CollegeWuhu 241000China Institute of Basic Research in Clinical Medicine China Academy of Chinese Medical SciencesBeijing 100700China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of EducationDepartment of Medicinal Plant DevelopmentChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100193China Department of Gastroenterology Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing 100091China

OBJECTIVE:To explore the functional role of the drugdependent mesenchymal-epithelial transition(Met)-axiation"π"structural module of neurogenesis after processing by three components of Qingkailing injection in neurogenesis and angiogenesis in cerebral ***:We used a Glutathione S-transferase(GST)-pull down assay,isothermal titration calorimetry assay,and other related methods to identify the relationships among Met,inositol polyphosphate phosphatase like 1(Inppl1),and death associated protein kinase 3(Dapk3)in this allosteric *** biological effects of the modules of neurons generation composed of Met,Inppl1,and Dapk3 were measured through Western blot,apoptosis analysis,and double immunofluorescence ***:The GST-pull down assay revealed that proline-serine-threonine rich domain of Met binds to the Src homology domain of Inppl1 to form a protein-protein complex;Dapk3 with a C-terminal domain interacts weakly with the protein kinase C domain of Met in the intracellular ***,we obtained a“π”structuring module considered a neural regeneration *** biological effects of angiogenesis and neurogenesis modules composed of Met,Inppl1,and Dapk3 were also ***:The study suggested that understanding the functional modules that contribute to pharmaceutics might provide novel signatures that can be used as endpoints to define disease processes under stroke or cerebral ischemia conditions.

关键词： brain ischemia stroke neurogenesis angiogenesis modular pharmacology Met-axiation allosteric module

Reconstruction of the most recent common ancestor sequences of SARS-Cov S gene and detection of adaptive evolution in the spike protein

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Chinese Science Bulletin 2004年第12期49卷 1311-1313页

作者： ZHANG Yuan1, ZHENG Nan2,3, HAO Pei4.5 & ZHONG Yang6 1. Laboratory of Systematic and Evolutionary Botany, Institute of Bot- any, Chinese Academy of Sciences, Beijing 100093, china 2. College of Life Sciences, Beijing Normal university, Beijing 100875, china 3. Institute of Viral Disease Control and Prevention, Chinese CDC, Beijing 100052, china 4. Bioinformation {4., Shanghai Institutes for biological Sciences, Chinese Academy of Sciences, Shanghai 200031, china 5. Shanghai {4. for Bioinformation Technology, Shanghai 200235, china 6. School of Life Sciences, Fudan university, Shanghai 2004.3, china 1. Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences 100093 Beijing China 2. College of Life Sciences Beijing Normal University 100875 Beijing China 3. Institute of Viral Disease Control and Prevention Chinese CDC 100052 Beijing China 4. Bioinformation center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 200031 Shanghai China 5. Shanghai center for Bioinformation Technology 200235 Shanghai China 6. School of Life Sciences Fudan University 200433 Shanghai China

The genome oiganization and expression strategy of severe acute respiratory syndrome coronavirus (SARS-CoV) have been described extensively. As a structural glycoprotein on the virion surface, the spike protein is responsible for binding to host cellular receptors and for the fusion between the viral envelope and the cellular membrane. It also induces neutralizing antibodies in the host and mediates cellular immunity. Previous studies suggested that amino acid replacements in the spike protein coulddramatically alter the pathogenesis and virulence of some coronaviruses. It is therefore reasonable to test the hypothesis that radical amino acid replacements in the spike protein, favored by environmental selective pressure during the process of SARS-CoV interspecific transmission, might make this pathogen adapt to a new host, In this study, we investigated a total of 108 complete sequences of the SARS-CoV S gene from Gen-Bank (until March 23, 2004.. After omission of those records containing frame-shift mutations or low quality sequences, e.g. ZJ01, and selection of one sequence for identical records, an alignment of 4. sequences was obtained using the program Clustal-X. Then, we reconstructed the most recent common ancestor (MRCA) sequences of theSARS-CoV S gene and detected the adaptive evolution in the spike protein.

关键词： SARS冠状病毒自适应进化基因表达糖蛋白分子免疫学波峰病毒

General gambogic acids inhibited growth of human hepatoma SMMC-7721 cells in vitro and in nude mice

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Acta Pharmacologica Sinica 2004年第6期25卷 67-72页

作者： Qing-long GUO2, Qi-dong YOU3, Zhao-qiu WU, Sheng-tao YUAN4.Li ZHAO Department of Physiology, Department of Medicinal Chemistry, china Pharmaceutical university, Nanjing 210009 3 4.nstitute of Materia Medica, Shanghai Institutes for biological Sciences, ChineseAcademy ofSciences, Shanghai 200031, china Department of Physiology Department of Medicinal Chemistry China Pharmaceutical University Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

AIM: To study the inhibitory effect of general gambogic acids (GGA) on transplantation tumor SMMC-7721 in experimental animal model and SMMC-7721 cells in vitro. METHODS: Anti-tumor activity of GGA in the experi- mental transplantation tumor SMMC-7721 was evaluated by relative tumor growth ratio. Cell morphology was observed with inverted microscope and electron microscope. Cell proliferation was measured by MTT assay and the telomerase activity was determined by PCR. RESULTS: In vivo study indicated that GGA (2, 4. and 8 mg/kg, iv, 3 times per week for 3 weeks) displayed an inhibitory effect on the growth of transplantation tumor SMMC- 7721 in nude mice compared with the normal saline group (P<0.01). At the concentrations of 0.625-5.0 mg/L, GGA remarkably inhibited the proliferation of SMMC-7721 cells in vitro. GGA 2 mg/L dramatically changed morphology of SMMC-7721 cells and inhibited the telomerase activity in SMMC-7721 cells. CONCLUSION: GGA had inhibitory effect on the growth of SMMC-7721, which might be related to its inhibition of telomerase activity.

关键词： gamboge gambogic acid hepatoma SMMC-7721 cells telomerase nude mice

Changes of microbial community structures and functional genes during biodegradation of phenolic compounds under high salt condition

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Journal of Environmental Sciences 2009年第6期21卷 821-826页

作者： WANG Ping,QU Yuanyuan,ZHOU Jiti School of Environmental and biological Science and Technology,Dalian university of Technology,Dalian 116024.china. [a]School of Environmental and biological Science and Technology Dalian University of Technology Dalian 116024 China

The changes of microbial community structures and functional genes during the biodegradation of single phenol and phenol plus p-cresol under high salt condition were *** was found that the phenol-fed system（PFS） exhibited stronger degrading abilities and more stable biomass than that of the phenol plus p-cresol-fed system（PCFS）.The microbial community structures were revealed by a modern DNA fingerprint technique,ribosomal intergenic spacer analysis（RISA）.The results indicated that the microbial community of PFS changed obviously when gradually increased phenol concentration,while PCFS showed a little change.16S rRNA sequence analysis of the major bands showed that Alcanivorax *** was predominant species during phenolic compounds ***,amplified functional DNA restriction analysis（AFDRA） on phenol hydroxylase genes showed that the fingerprints were substantially different in the two systems,and the fingerprints were not the same during the different operational periods.

关键词： phenolic compounds high salt phenol hydroxylase ribosomal intergenic spacer analysis amplified functional DNArestriction analysis

A non-commutative time-frequency tomography

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Chinese Science Bulletin 2007年第17期52卷 2438-2442页

作者： LIU JianCai, XU JiaKai, NING XinBao & TIAN Run Key Laboratory of modern Acoustics, Department of Electronics Science and Engineering Institute of biological Medical Electronic Engineering, Nanjing university, Nanjing 210093, china Key Laboratory of modern Acoustics Department of Electronics Science and Engineering Institute of biological Medical Electronic Engineering Nanjing University Nanjing 210093 China

The characterization of non-stationary signal requires joint time and frequency information. However, time and frequency are a pair of non-commuting variables that cannot constitute a joint probability density in the time-frequency plane. The time-frequency distributions have difficult interpretation problems arising from negative and complex values or spurious components. In this paper, we get time-frequency information from the marginal distributions in rotated directions in the time-frequency plane. The rigorous probability interpretation of the marginal distributions is without any ambiguities. This time-frequency transformation is similar to the computerized axial tomography (CT or CAT) and is applied to signal analysis and signal detection and reveals a lot of advantages especially in the signal detection of the low signal/noise (S/N).

关键词：非交换时频X线断层摄影术边缘分布医学影像学时频分布

Systemic PCD occurs in TMV-tomato interaction

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Science china(Life Sciences) 2008年第11期51卷 1009-1019页

作者： ZHOU ShuMin, LIU WenNa, KONG LinAn & WANG Mao College of biological Sciences, china Agriculture university, Beijing 100094. china 1. College of biological Sciences China Agriculture University Beijing 100094 China

In hypersensitive response (HR), programmed cell death (PCD) is reported as a powerful defense mechanism in plant immune responses to pathogen. However, little is known about the PCD in sys-temic acquired resistance (SAR). Using tobacco mosaic virus (TMV) to infect the tomato (Lycopersicon esculentum cv. Jiafen 16) we found that localized TMV-infection could induce cell death in the uninoculated parts of the tomatoes, where the enzyme-linked immunosorbent assay (ELISA) showed no spreading virus. The biological and molecular characterization of this cell death was shown as fol-lowing: chromatin condensed and formed peripheral conglomeration in nuclei; cell nucleus were TUNEL positive labeled; genomic DNA was fragmented and showed DNA laddering; mitochondria and chloroplast were disrupted; tonoplast and plasma membrane were shrunk and degradated. These re-sults suggested that with an absence of TMV spread, the local TMV-infection on certain tomato leaves could induce systemic PCD in the root-tips, stem-apices and uninoculated leaves. The systemic PCD has various initiation and synchronization in such tissues and is distinct in inducement and exhibition from HR-PCD and SAR.

关键词： programmed cell death systemic PCD TUNEL assay DNA ladder TMV tomato

Semantic Search among Heterogeneous biological Databases Based on Gene Ontology

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Acta Biochimica et Biophysica Sinica 2004年第5期36卷 365-370页

作者： Shun-Liang CAO, Lei QIN1, Wei-Zhong HE1, Yang ZHONG2, Yang-Yong ZHU, and Yi-Xue LI1,3* Department of Computing and Information Technology, Fudan university, Shanghai 2004.3, china Shanghai center for Bioinformation 1 Technology, Shanghai 200235, china School of Life Sciences, Fudan university, Shanghai 2004.3, china Bioinformation center of Shanghai Institutes for biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, china Department of Computing and Information Technology Fudan University Shanghai China

Semantic search is a key issue in integration of heterogeneous biological databases. In this paper, we present a methodology for implementing semantic search in BioDW, an integrated biological data warehouse. Two tables are presented: the DB2GO table to correlate Gene Ontology (GO) annotated entries from BioDW data sources with GO, and the semantic similarity table to record similarity scores derived from any pair of GO terms. Based on the two tables, multifarious ways for semantic search are provided and the corresponding entries in heterogeneous biological databases in semantic terms can be expediently searched.

关键词： Gene Ontology semantic search BioDW

cDNA cloning and recombinant expression of the general odorant binding protein Ⅱ from Spodoptera litura

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Science china(Life Sciences) 2009年第1期52卷 80-87页

作者： JIN FengLiang,DONG XiaoLin,XU XiaoXia & REN ShunXiang Engineering Research center of biological Control,College of Natural Resources and Environment,South china Agricultural university,Ministry of Education,Guangzhou 51064.,{4. 1. Engineering Research center of biological Control College of Natural Resources and Environment South China Agricultural University Ministry of Education Guangzhou 510642 China

A cDNA encoding the general odorant binding protein Ⅱ(GOBP Ⅱ) was isolated from the antennae of Spodoptera litura(SlGOBP Ⅱ,GenBank Accession ***086371) by homologous cloning and rapid amplification of cDNA ends(RACE).Sequencing and structural analyses revealed that the open reading frame(ORF) of SlGOBP Ⅱ was 4.9 bp,encoding 162 amino acids with a predicted MW of 18.2 kD and pI of *** Ⅱ shared typical structural features of odorant binding proteins with other insects,including the six conservative cysteine *** deduced amino acid sequence of SlGOPB Ⅱ shared significant identity with the GOBP Ⅱ from *** and ***-PCR and Northern blot analyses showed that SlGOBP Ⅱ was specifically expressed in the *** encoding SlGOBP Ⅱ was constructed into the pET-32a vector and the recombinant protein was highly expressed in Escherichia coli BL21(DE3) after induction with *** electrophoresis and Western blot analysis confirmed the molecular weight of the recombinant SIGOBPⅡ i.e,32 kD,which has a 6×His tag at the *** recombinant SlGOBP Ⅱ was purified by single-step Ni-NTA affinity chromatography and used to raise antiserum in *** showed that the titer of antiserum was 1:12800,while Western blot analysis showed that the recombinant SlGOBP Ⅱ was recognized as anti-SlGOBP Ⅱ antiserum.

关键词： Spodoptera litura antennae general odorant binding protein gene cloning prokaryotic expression