Changes of microbial community structures and functional genes during biodegradation of phenolic compounds under high salt condition
Changes of microbial community structures and functional genes during biodegradation of phenolic compounds under high salt condition作者机构:School of Environmental and Biological Science and Technology Dalian University of Technology Dalian 116024 China
出 版 物:《Journal of Environmental Sciences》 (环境科学学报(英文版))
年 卷 期:2009年第21卷第6期
页 面:821-826页
核心收录:
学科分类:0830[工学-环境科学与工程(可授工学、理学、农学学位)] 07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学]
基 金:supported by the National Natural Science Foundation of China (No. 50608011)
主 题:phenolic compounds high salt phenol hydroxylase ribosomal intergenic spacer analysis amplified functional DNArestriction analysis
摘 要:The changes of microbial community structures and functional genes during the biodegradation of single phenol and phenol plus p-cresol under high salt condition were *** was found that the phenol-fed system(PFS) exhibited stronger degrading abilities and more stable biomass than that of the phenol plus p-cresol-fed system(PCFS).The microbial community structures were revealed by a modern DNA fingerprint technique,ribosomal intergenic spacer analysis(RISA).The results indicated that the microbial community of PFS changed obviously when gradually increased phenol concentration,while PCFS showed a little change.16S rRNA sequence analysis of the major bands showed that Alcanivorax *** was predominant species during phenolic compounds ***,amplified functional DNA restriction analysis(AFDRA) on phenol hydroxylase genes showed that the fingerprints were substantially different in the two systems,and the fingerprints were not the same during the different operational periods.