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Expression,Characterization and Immunogenicity of a Major Outer Membrane Protein from Vibrio alginolyticus

Acta Biochimica et Biophysica Sinica 2007年第3期39卷 194-200页

作者： Ronghua QIAN~(1,2) Wuying CHU~3 Zhijuan MAO~(1,4) Chongwen ZHANG~(1,2) Yongwei WEI~2 Lian YU~2* 1 Department of Biomedical Engineering,Zhejiang University,Hangzhou 310027,china 2 institute of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 310029,china 3 institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,china 4 ningbo oceanography and fishery institute,ningbo 315012,china DepartmentofBiomedicalEngineering ZhejiangUniversityHangzhou310027China instituteofPreventiveVeterinaryMedicine ZhejiangUniversityHangzhou310029China instituteofSubtropicalAgriculture ChineseAcademyofSciencesChangsha

Vibrio alginolyticus is one of the Vibrio pathogens common to humans and marine *** infection and induction of the host immune response,outer membrane proteins of bacteria play animportant *** this study,an outer membrane protein gene(ompW)was cloned from *** andexpressed in Escherichia *** 645 bp open reading frame(ORF)encodes a protein of 214 amino acidresidues with a predicted molecular weight of 23.3 *** amino acid sequence showed a high identitywith that of Photobacterium damselae(96.2%)and Vibrio parahaemolyticus(94.4%).The alignment analy-sis indicated that OmpW was highly *** dodecyl sulfate-polyacrylamide gel electrophoresisshowed that the gene was over-expressed in *** BL21(DE3).Western blot analysis revealed that theexpressed protein had *** recombinant protein was purified by affinity chromatographyon Ni-NTA Superflow *** yellow croaker vaccinated with the purified OmpW showed significantlyincreased antibody to OmpW,which could resist the infection by ***.A specific antibody wasdetected by enzyme-linked immunosorbent *** study suggested that the conserved OmpW could bean effective vaccine candidate against infection by ***.

关键词： Vibrio alginolyticus ompW expression characterization immunogenicity vaccine candidate

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An operational satellite remote sensing system for ocean fishery

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Acta Oceanologica Sinica 2004年第3期23卷 427-436页

作者： MAOZhihua ZHUQiankun PANDelu Secondinstituteofoceanography StateOceanicAdministrationHangzhou310012China KeyLaboratoryofOceanDynamicProcessesandSatelliteoceanographyofStateOceanicAdministration Hangzhou310012China

Ocean environmental information is very important to supporting the fishermen in fishing and satellite remote sensing technology can provide it in large scale and in near real-time. Ocean fishery locations are always far away beyond the coverage of the satellite data received by a land-based satellite receiving station. A nice idea is to install the satellite ground station on a fishing boat. When the boat moves to a fishery location, the station can receive the satellite data to cover the fishery areas. One satellite remote sensing system was once installed in a fishing boat and served fishing in the North Pacific fishery areas when the boat stayed there. The system can provide some oceanic environmental charts such as sea surface temperature (SST) and relevant derived products which are in most popular use in fishery industry. The accuracy of SST is the most important and affects the performance of the operational system, which is found to be dissatisfactory. Many factors affect the accuracy of SST and it is difficult to increase the accuracy by SST retrieval algorithms and clouds detection technology. A new technology of temperature error control is developed to detect the abnormity of satellite-measured SST. The performance of the technology is evaluated to change the temperature bias from -3.04 to 0.05 ℃ and the root mean square (RMS) from 5.71 to 1.75℃. It is suitable for employing in an operational satellite-measured SST system and improves the performance of the system in fishery applications. The system has been running for 3 a and proved to be very useful in fishing. It can help to locate the candidates of the fishery areas and monitor the typhoon which is very dangerous to the safety of fishing boats.

关键词： fishery oceanography charts satellite remote sensing sea surface temperature temperature error control technology

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Study on fish community structure in the Zhujiang Estuaryand its adjacent waters

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Acta Oceanologica Sinica 1999年第4期18卷 581-589页

作者： Zhan Haigang( South china As Fisheries institute, Chinese Academy of fishery Sciences, Guangzhou 510300, china) South china Sea Fisheries institute Chinese Academy of Fishery Sciences Guangzhou 510300 China

Based on data of demersal trawl Samples from 1987 to 1988 in the Zhujiang Estuary and its adjacent waters, relationships between fish community and environmental factors are studied by using clssification , MDS ordina-tion and several community parameters. The results shaw that the distribution of fish shoes is zoned by salinty anddivided into three types. The freshwater community which is restricted in the Lianhua Hill - Humen waterway is sim-ple and stable, the coastal community which lives in the shallow waters is complex, and the estuary community whichis idenhfied in the Lingdingyang area and affected by Physical and ecological pinemore greatly greatly has obvious seasonalvariations.

关键词： Zhujiang Estuary fish community structure ordination

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Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean

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Acta Oceanologica Sinica 2003年第4期22卷 643-649页

作者： Lin Xuezheng1,Yang Xiuxia2,Bian Ji1,Huang Xiaohang1 1. First institute of oceanography,State Oceanic Administration,Qingdao 266061,china 2. fishery College, Ocean University of china,Qingdao 266003,china State Ocean Adm Inst Oceanog 1 Qingdao 266061 Peoples R China Ocean Univ china Fishery Coll Qingdao 266003 Peoples R China

A strain of psychrophilic bacterium, 2-5-10-1, which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern Ocean. The highest lipase secretion of this strain is observed at 5 degreesC and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 35 degreesC and 7.5 degreesC respectively. At 0degreesC, the lipase still has 37% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 degreesC for 15 min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu2+ and Zn2+.

关键词： Southern Ocean deep sea psychrophilic bacteria low-temperature lipase

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Research on isozymes in various organs of Perineresis aibuhitensis （Polychaeta:Nereididae）

Research on isozymes in various organs of Perineresis aibuhi...

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2005水产科技论坛

作者： JIANG Xia-min, LIU Min-hai (Ocean and fishery Department, ningbo University, ningbo 315211, china Fisheries institute of Zhoushan Zhejiang, Zhoushan 316000, china)

＜正＞The patterns of allozyme polymorphisms in various organs of Perinere-sis aibuhitensis were investigated. Population was collected from the East Sea coast of Wenling of Zhejiang Province, china. Fourteen isozymes in six tissues or organs;head, pharynx, esophagus, intestine, muscle and parapodium of Perineresis ai6u-hitensis were studied by using the vertical polyacrylamide gel electrophoresis, the phe-notypes and the expressions of the isozymes were analyzed. The results showed that CAT isozymes did not express in the head and D-GCDH isozymes did not express in the pharynx. The SOD isozyme existed in all these tissues. ADH, ATP, EST, FDH, GAD, GDH, LDH, MDH, ME, POD, SCD and SDH isozymes exhibited an apparent tissues-specificity.

关键词： Perineresis aibuhitensis isozymes electrophoresis tissues-specificity

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Protochordate amphioxus is an emerging model organism for comparative immunology

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Progress in Natural Science:Materials International 2009年第8期19卷 923-929页

作者： Shicui Zhang a, Yujun Liang a, Guangdong Ji a, Zhimeng Zhuang b a Department of Marine Biology, Ocean University of china, Qingdao 266003, china b Yellow Sea fishery Research institute, Chinese Academy of fishery Science, Qingdao 266071, china [a]Department of Marine Biology Ocean University of China Qingdao 266003 China [b]Yellow Sea Fishery Research Institute Chinese Academy of Fishery Science Qingdao 266071 China

Protochordate amphioxus is an extant invertebrate regarded quite recently as a basal chordate. It has a vertebrate-like body plan including a circulation system with an organization similar to that of vertebrates. However, amphioxus is less complex than vertebrates for having a genome uncomplicated by extensive genomic duplication, and lacking lymphoid organs and free circulating blood cells. Recent studies on immunity have demonstrated the presence in amphioxus of both the constituent elements of key molecules involved in adaptive immunity such as proto-major histocompatibility complex (proto-MHC), V region-containing chitin-binding protein (VCBP) and V and C domain-bearing protein (VCP), and the complement system operating via the alternative and lectin pathways resembling those seen in vertebrates. In addition, the acute phase response profile in amphioxus has been shown to be similar to that observed in vertebrates. These findings together with the relative structural and genomic simplicity make amphioxus an ideal organism for gaining insights into the origin and evolution of the vertebrate immune system, especially adaptive immunity, and the composition and mech- anisms of the vertebrate innate immunity.

关键词： Amphioxus Branchiostoma Immunity Evolution

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Effects of artificial regulations of rotifers n-3 HUFA content on the growth and survival of larval black sea bream ( Sparus macrocephalus)

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Chinese Science Bulletin 1997年第13期42卷 1130-1132页

作者： LIU Jingke and LEI Jilin1. institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, china 2 Yellow Sea fishery Research institute Qingdao 266003, china institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China

STUDIES on the nutrition requirement of larval fish have made some progress abroad, but therehave been no reports on such studies in china. It was determined recently that n-3 HUFA (es-pecially 22: 6(n-3) (DHA) and 20:5 (n-3) (EPA)) is essential for larval seafish growth. Thehigh content of DHA in seafish eggs and the low content of DHA in rotifers show that rotifersas live food cannot satisfy the n-3 HUFA requirement of larval seafish. Some scientists ob-

关键词： rotifer (Brachionus phicatilis) highly unsaturated fatty acid (HUFA) larval black sea bream.

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微卫星标记在坛紫菜丝状体品系DNA指纹构建中的应用

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水产学报 2005年第3期29卷 323-326页

作者：刘必谦曾庆国骆其君周湘池宁波大学海洋生物工程重点实验室宁波海洋与渔业研究院宁波大学海洋生物工程重点实验室浙江宁波315211 浙江宁波315012

用从条斑紫菜EST数据库中筛选合成的微卫星引物对8个坛紫菜丝状体品系进行微卫星DNA指纹扩增。5个微卫星引物共扩增出32条带,其中3对引物所扩增出的5个条带(AU192094-127、AU187410-335、AU187410-190、AU194267-203和AU194267-328)被... 详细信息

用从条斑紫菜EST数据库中筛选合成的微卫星引物对8个坛紫菜丝状体品系进行微卫星DNA指纹扩增。5个微卫星引物共扩增出32条带,其中3对引物所扩增出的5个条带(AU192094-127、AU187410-335、AU187410-190、AU194267-203和AU194267-328)被用来构建8个坛紫菜丝状体品系的DNA指纹。在这个图谱中,每个丝状体品系都有独一的指纹模式,彼此很容易被区分开。所获得的DNA指纹图谱,可用来进行孟德尔分离研究,及为坛紫菜纯系鉴定提供分子基础。

关键词： DNA指纹微卫星标记坛紫菜丝状体

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Long-term changes of phytoplankton community in Xiagu waters of Xiamen,china

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Acta Oceanologica Sinica 2010年第6期29卷 104-114页

作者： CHEN Baohong XU Zhuhua ZHOU Qiulin CHEN Changping GAO Yahui YANG Shengyun JI Weidong School of Life Sciences Xiamen University Xiamen 361005 China Third institute of oceanography State Oceanic Administration Xiamen 361005 China Marine Environment and fishery Resources Monitoring Center of Fujian Province Fuzhou 350003 China Department of oceanography Xiamen University Xiamen 361005 China

Long-term changes of phytoplankton community by water sampling method in Xiagu Sea waters of Xiamen,china,were investigated in this *** composition of the phytoplankton community in these waters changed greatly since the *** numbers of Dinophyta species increased significantly,although Bacillariophyta species are generally *** succession of dominant species in phytoplankton community is obvious： large-size dominant species such as Biddulphia sinensis of the 1950s were gradually replaced by small-size ones such as Cyclotella striata and Nitzschia closterium,and species that still maintain dominant such as Skeletonema costatum are also small ones,leading the whole phytoplankton community of smaller *** density of phytoplankton community increased greatly,among which cell density of the most dominant species Skeletonema costatum have been increasing in exponent *** index of phytoplankton community decreased,indicating decline of biodiversity of the community,and dominant character of Skeletonema costatum ***,the structure of the entire phytoplankton community is becoming more and more singular and unstable,which makes the occurrence of red tides more *** succession in the phytoplankton community is related to the long-term changes in marine environment,influenced by human activities and global climate changes,especially the increases of nutrient content.

关键词： phytoplankton community structure Xiamen water sampling long-term

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Construction of AFLP-based genetic linkage maps for the Chinese shrimp Fenneropaeneus chinensis

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Chinese Science Bulletin 2008年第8期53卷 1205-1216页

作者： TIAN Yi1,2,3,4, KONG Jie1↑ & WANG WeiJi1 1 Yellow Sea Fisheries Research institute, Chinese Academy of fishery Science, Qingdao 266071, china 2 College of Life Science and Technology, Dalian Fisheries University, Dalian 116023, china 3 institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, china 4 Graduate University of Chinese Academy of Sciences, Beijing 100049, china Chinese Acad Fisheries Sci Yellow Sea Fisheries Res Inst Qingdao 266071 Peoples R China.

Fenneropaeneus chinensis is an important species in marine fishery resources and aquaculture in china. A genetic linkage map is essential for improving the efficiency of its breeding by marker-as- sisted selection and identifying commercially important genes. Linkage maps of F. chinensis were constructed with an F2 mapping population (110 progenies) using amplified fragment length polymor- phic (AFLP) marker in this study. Fifty-five AFLP primer combinations produced 532 AFLP markers fitting for map strategy in mapping family. The markers with 3:1 segregating ratios were analyzed using F2 intercross model for the common linkage map, while the markers with 1:1 ratio were analyzed using the pseudo-testcross strategy. The maps of male, female and common were constructed. The female map included 103 markers that formed 28 linkage groups, covering a total length of 1090 cM. All mark- ers were linked with the linkage groups. Segregation distortion was observed for 6 of 103 markers in the female map. The average distance between markers was 14.53 cM and ranged from 4.4 to 24.8 cM. The male map included 144 markers that formed 35 linkage groups. Ten markers remained unlinked in male map. Segregation distortion was observed for 7 of 144 markers in the male map. The total dis- tance of male map covered 1617 cM. The average distance between markers was 16.36 cM. The male map was 32.6% longer than the female map, which may reflect sex-specific recombination rates in Chinese shrimp. The common map was composed of 216 markers, including in 44 linkage groups covering a total distance of 1772.1 cM. Two markers remained unlinked. No distorted markers of 216 markers were shown in the common map. The distance between markers was 10.42 cM. An average estimated genome size for the Chinese shrimp was 2420 cM, which was consistent with the relative size of the Penaeid genome. The distribution of AFLP markers was relatively even in chromosomes of Chi- nese shrimp maps. The linkage analysis

关键词： AFLP 海洋渔业虾中国

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