Construction of AFLP-based genetic linkage maps for the Chinese shrimp Fenneropaeneus chinensis

作     者：TIAN Yi1,2,3,4, KONG Jie1↑ & WANG WeiJi1 1 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China 2 College of Life Science and Technology, Dalian Fisheries University, Dalian 116023, China 3 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 4 Graduate University of Chinese Academy of Sciences, Beijing 100049, China 

作者机构：Chinese Acad Fisheries Sci Yellow Sea Fisheries Res Inst Qingdao 266071 Peoples R China. op.uky.edu 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2008年第53卷第8期

页      面：1205-1216页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：the National ‘948’ Project (Grant No. 2006-G55(B)) the National High Technology Research and Development Program of China (Grant No. 2006AA10A406) 

主　　题：AFLP 海洋渔业 虾 中国 

摘      要：Fenneropaeneus chinensis is an important species in marine fishery resources and aquaculture in China. A genetic linkage map is essential for improving the efficiency of its breeding by marker-as- sisted selection and identifying commercially important genes. Linkage maps of F. chinensis were constructed with an F2 mapping population (110 progenies) using amplified fragment length polymor- phic (AFLP) marker in this study. Fifty-five AFLP primer combinations produced 532 AFLP markers fitting for map strategy in mapping family. The markers with 3:1 segregating ratios were analyzed using F2 intercross model for the common linkage map, while the markers with 1:1 ratio were analyzed using the pseudo-testcross strategy. The maps of male, female and common were constructed. The female map included 103 markers that formed 28 linkage groups, covering a total length of 1090 cM. All mark- ers were linked with the linkage groups. Segregation distortion was observed for 6 of 103 markers in the female map. The average distance between markers was 14.53 cM and ranged from 4.4 to 24.8 cM. The male map included 144 markers that formed 35 linkage groups. Ten markers remained unlinked in male map. Segregation distortion was observed for 7 of 144 markers in the male map. The total dis- tance of male map covered 1617 cM. The average distance between markers was 16.36 cM. The male map was 32.6% longer than the female map, which may reflect sex-specific recombination rates in Chinese shrimp. The common map was composed of 216 markers, including in 44 linkage groups covering a total distance of 1772.1 cM. Two markers remained unlinked. No distorted markers of 216 markers were shown in the common map. The distance between markers was 10.42 cM. An average estimated genome size for the Chinese shrimp was 2420 cM, which was consistent with the relative size of the Penaeid genome. The distribution of AFLP markers was relatively even in chromosomes of Chi- nese shrimp maps. The linkage analysis