Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean

作     者：Lin Xuezheng1,Yang Xiuxia2,Bian Ji1,Huang Xiaohang1 1. First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China 2. Fishery College, Ocean University of China,Qingdao 266003,China 

作者机构：First Institute of Oceanography State Oceanic Administration Qingdao 266061 China Fishery College Ocean University of China Qingdao 266003 China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2003年第22卷第4期

页      面：643-649页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：hisstudywassupportedby 10thfive yearplan (DY10 5 0 4 0 2 0 3)ofChinaOceanMineralResourcesR&DAssociation 

主　　题：Southern Ocean deep sea psychrophilic bacteria low-temperature lipase 

摘      要：A strain of psychrophilic bacterium, 2-5-10-1, which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern Ocean. The highest lipase secretion of this strain is observed at 5 degreesC and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 35 degreesC and 7.5 degreesC respectively. At 0degreesC, the lipase still has 37% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 degreesC for 15 min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu2+ and Zn2+.