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High-and Low-Frequency Probe Tone Tympanometry in Chinese Infants

Journal of Otology 2007年第1期2卷 42-51页

作者： SHANG Ying-ying1, NI Dao-feng1, LIU Shi-lin2 1 Department of Otorhinolaryngology, Peking union Medical college Hospital, Chinese Academy of Medical sciences and Peking union Medical college, beijing, china 2 Department of Otorhinolaryngology, beijing Children’s Hospital, Capital Medical university, beijing, china Department of Otorhinolaryngology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China Department of Otorhinolaryngology Beijing Children’s Hospital Capital Medical University Beijing

Objective To assess the utility of low- and high-frequency tympanometry in the diagnosis of middle ear dysfunction in Chinese infants. Methods Tympanograms were obtained with 226 Hz, 678 Hz and 1000 Hz probe tones from infants aged 5-25 weeks with normal auditory brainstem responses(ABRs)(15 infants, 3. ears) and with prolonged wave I latencies(17 infants, 20 ears), suggesting middle ear dysfunction, using a GSI Tympstar middle ear analyzer Version II. Results The single-peaked tympanogram was the most characteristic type in both groups and seen in 25 ears (83.3.) in the normal ABR group and in 18 ears (90%) in the delayed wave I group, respectively. The peak pressure, peak compensated static acoustic admittance and gradient of 226 Hz tympanometry were of no significant differences between the two groups. The 678 Hz tympanograms of admittance, susceptance and conductance demonstrated non-peak, single-, double- and tri-peaked patterns in both groups. The agreement between ABRs and 678 Hz tympanograms of admittance,susceptance and conductance were 70.0%, 58.0% and 64.0%(kappa=0.3.4, 0.23. and 0.118) respectively. For 1000 Hz probe tone, admittance, susceptance and conductance tympanograms showed single peaked patterns in 28(93.3.), 25 (83.3.) and 26(86.7%) of the 3. normal ears. Admittance, susceptance and conductance tympanograms using the 1000 Hz probe tone were flat in 15 (75%), 17(85%) and 13.(65%) of the ears in infants with prolonged wave I latencies. For 1000 Hz admittance, susceptance and conductance Tympanograms, the agreement between tympanometry and ABR results were 90.0%, 92.0% and 86.0% with kappa at 0.783. 0.83. and 0.690, respectively. Conclusion 1000 Hz probe tone tympanometry is a promising middle ear function test for infants of 1-6 months age, while 226 Hz and 678 Hz probe tones are less efficient in detecting middle ear dysfunction in infants.

关键词： ABR High-and Low-Frequency Probe Tone Tympanometry in Chinese Infants

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The Induction of Liver Microsomal Cytochrome P450 by Glycyrrhiza uralensis and Glycyrrhetinic acid in Mice

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Biomedical and Environmental sciences 1999年第1期12卷 10-14页

作者： HU WEN-YU LI YA-WEI HOU YAN-NING HE KAN (Department of Pharmacology, Instiute of Materia Medica, Chinese Academy of Medical Scicnces & beijing union Medical college, beijing 100050, china) CHEN JIE-FU (Yang-Ming Medical university, Taipei) BUT PAUL PU Department of Pharmacology Chinese Academy of Medical Sciences & Beijing Union Medical College China

The effect of Glycyrrhiza uralensis Fisch (GRZ) aqueous extract and one of its active principles Glycyrrhetinic acid (GRT) on hepatic cytochrome P450 in mice were investigated. Oral administration of GRZ at 10g/kg/d or GRT at 50 mg/kg/d for 7 days was foulld to increase the P450 contents up to 4.6 fold compared with the controls. The activities of aryl hydrocarbon hydroxylase (AHH,3. 1 and 3. 3.fold), aminopyrine N-demethylase (ADM, 4. 2 and 3. 2 folds), and 7-ethoxycumarin O-deethylase (ECOD, 2. 8 and 2. 5 fold) were also shown to be increased. Westem blot analysis showed that the subtypes of P450 isoforms induced selectively by GRZ and GRT included CYP1A1 (1. 8 and 1. 5 fold over that of the control, respectively), CYP2B1 (both 1.3.fold), and CYP2C11(3..2 and 3..0 fold). Moreover, significant positive correlation between the P450 content or the isoforms and the corresponding enzyme activities mentioned above was observed.

关键词： Plants Medicinal Animals Blotting Western Cytochrome P-450 Enzyme System Enzyme Induction Glycyrrhetinic Acid Isoenzymes Male Mice Microsomes Liver

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计算机基础课程开展综合实验设计的教学实践

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实验技术与管理 2009年第6期 203-204页

作者：于宁北京联合大学应用文理学院信息科学与技术系

培养应用型人才需要调动学生的积极性,关键在于为学生提供一个良好的教学实践环境。对计算机基础教研室所承担的3.计算机公共基础课程进行了全方位建设,重点注重教学过程中的实践环节,开展综合实验设计,结合今后工作中的需求,充分利用... 详细信息

培养应用型人才需要调动学生的积极性,关键在于为学生提供一个良好的教学实践环境。对计算机基础教研室所承担的3.计算机公共基础课程进行了全方位建设,重点注重教学过程中的实践环节,开展综合实验设计,结合今后工作中的需求,充分利用课堂所学知识,充分发挥学生的创造性,真正体现'应用为本,学以致用'。

关键词：教学实践综合实验设计面向应用

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Assessment of candidate plant DNA barcodes using the Rutaceae family

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Science china(Life sciences) 2010年第6期53卷 701-708页

作者： LUO Kun1,2,CHEN ShiLin1,CHEN KeLi2,SONG JingYuan1,YAO Hui1,MA XinYe1,ZHU YingJie3.PANG XiaoHui1,YU Hua1,LI XiWen1,4 & LIU Zhen2 1Institute of Medicinal Plant Development,Peking union Medical college,Chinese Academy of Medical sciences,{3. 100193.china 2college of Pharmacy,Hubei university of Chinese Medicine,Wuhan 43.061,china 3.chool of Bioscience and Engineering,Southwest Jiaotong university,Chengdu 61003.,china 4Department of Chemistry,Tsinghua university,{3. 100084,china 1. Institute of Medicinal Plant Development Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100193 China2. College of Pharmacy Hubei University of Chinese Medicine Wuhan 430061 China3. School of Bioscience and Engineering Southwest Jiaotong University Chengdu 610031 China4. Department of Chemistry Tsinghua University Beijing 100084 China

DNA barcoding is a rapidly developing frontier technology that is gaining worldwide ***,seven regions (psbA-trnH,matK,ycf5,rpoC1,rbcL,ITS2,and ITS) with potential for use as DNA barcodes were tested for their ability to identify 3.0 samples of 192 species from 72 genera of the family *** evaluate each barcode’s utility for species authentication,PCR amplification efficiency,genetic divergence,and barcoding gaps were *** found that the ITS2 region exhibited the highest inter-specific divergence,and that this was significantly higher than the intra-specific variation in the "DNA barcoding gap" assessment and Wilcoxon two-sample *** ITS2 locus had the highest identification efficiency among all tested *** a previous study,we found that ITS2 was able to discriminate a wide range of plant taxa,and here we confirmed that ITS2 was also able to discriminate a number of closely related ***,we propose that ITS2 is a promising candidate barcode for plant species identification.

关键词： DNA barcoding ITS2 Rutaceae identification

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深化教学改革与文献检索课

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图书情报工作 1997年第11期41卷 50-52页

作者：王骊北京联合大学应用文理学院图书馆

分析深化教学改革中文献检索课停滞不前的主要原因，提出重视文献检索课。

关键词：教学改革人才培养情报检索

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Protein transduction domain of membrane penetrating peptide can efficiently deliver DNA and protein into mouse liver for gene therapy

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Hepatobiliary & Pancreatic Diseases International 2005年第1期4卷 90-93页

作者： Jun Xie, Bao-Feng Yu, Jun Xu, Yue-Hong Zhang, Niu-Liang Cheng, Bo Niu, Xiao-Nian Hu, Qian Xiang and Zheng-Guo Zhang Taiyuan, china Department of Biochemistry and Molecular Biology, Shanxi Medical university, Taiyuan 03.001 , china Peking {3. Medical college, Chi- nese Academy of Medical sciences, beijing 100005, china Department of Biochemistry and Molecular Biology Shanxi Medical University Taiyuan 030001 China. xiejunty@***

BACKGROUND: The development of a harmless and effi- cient nonviral gene delivery system that can facilitate the penetration of nucleic acids through the plasma membrane is a key to successful gene therapy. The aim of this study was to test a nonviral gene transferring vector's function of delivering DNA into liver cells to provide an important clue for gene transfer in liver gene therapy. METHODS: The complex of DNA and DNA delivering protein was injected into mice through their tail veins. Then the mice were killed and their liver tissue was sec- tioned. The gene transferring results were detected using a confocal laser scanning microscope. RESULTS: Fluorescence analysis indicated that both DNA- membrane penetrating peptide (MPP) complex and DNA- hepatocyte specific receptor binding domain ( HSRBD) - MPP complex could go into liver cells. The fluorescence value of liver cells in the DNA-HSRBD-MPP group was higher than that in the DNA-MPP group. CONCLUSIONS; MPP can successfully deliver DNA and protein into cells, and MPP with a HSRBD can specifically deliver DNA into liver cells. These have laid a foundation for further study on the nonviral liver cell gene delivering system.

关键词： membrane penetrating peptide gene therapy gene delivering

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Inhalable particle matter (PM10) can induce and aggravate asthmatic inflammation in rats

Inhalable particle matter (PM10) can induce and aggravate as...

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The 5th Congress of Toxicology in Developing Countries

作者： Xiao-Hong Zhao, Xin-Biao Guo, Shi-Jie Liu Department of Biology, college of arts and sciences of {3. union university Department of Occupational and Environmental Health sciences, School of Public Health, Peking university, beijing 100083. china

Inhalable particle matter (PM) is considered to play an important role in asthmatic airway inflammation. The aim of the present study was to investigate whether PM can induce or aggravate the asthmatic inflammation in sensitized rats. PM was collected on glass-fibre filters using a high-volume sampler, located nearly ur-

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New ancient DNA sequences suggest high genetic diversity for the woolly mammoth (Mammuthus primigenius)

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Progress in Natural Science:Materials International 2006年第4期16卷 379-386页

作者： YANG Shujuan, LAI Xulong, SHI Suhua, CHENG Shenghong, ZHOU Xiugao, YANG Xiujuan and YANG Hong Department of Earth Science, china university of Geosciences, Wuhan 43.074, china Department of Life Science, Sun Yat-Sen university, Guangzhou 510275, china Institute of Zoology, Chinese Academy of sciences, beijing 100080, china Heilongjiang Provincial Museum, Harbin 150001, china Department of sciences and Technology, college of arts and sciences, Bryant university, Smithfield, Rhode Island 02917, USA and Department of Geology and Geophysics, Yale university, New Haven, CT 06520, USA [a]Department of Earth Science China University of Geosciences Wuhan China [b]Institute of Zoology Chinese Academy of Sciences Beijing China [c]Department of Life Sceince Sun Yat-Sen University Guangzhou China [d]Heilongjiang Provincial Museum Harbin China [e]Department of sciences and Technology College of Arts and Sciences Bryant University Smithfield Rhode Island USA [f]Department of Geology and Geophysics Yale University New Haven CT USA

Partial DNA sequences of cytochrome b gene (mtDNA) were successfully retrieved from Late Pleistocene fossil bone of Mammuthus primigenius collected from the Xiguitu County ( Yakeshi), Inner Mongolia Autonomous Region and from Zhaodong, Harbin of Heilongjiang Province in northern china. Two ancient DNA fragments (109 bp and 124 bp) were authenticated by reproducible experiments in two different laboratories and by phylogenetic analysis with other Elephantidae taxa. Phylogenetic analysis using these sequences and published data in either separate or combined datasets indicate unstable relationship among the woolly mammoth and the two living elephants, Elephas and Loxodonta . In addition to the short sequences used to attempt the long independent evolution of Elephantidae terminal taxa, we suggest that a high intra-specific diversity existed in Mammuthus primigenius crossing both spatial and temporal ranges, resulting in a complex and divergent genetic background for DNA sequences so far recovered. The high genetic diversity in the extinct woolly mammoth can explain the apparent instability of Elephantidae taxa on the molecular phylogenetic trees and can reconcile the apparent paradox regarding the unresolved Elephantidae trichotomy.

关键词： Mammuthus primigenius genetic diversity ancient DNA phylogenetic analysis.

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应用型人才实践能力培养模式的探讨

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实验技术与管理 2009年第6期 169-171页

作者：孙建华北京联合大学应用文理学院实践教学基地

北京联合大学适应北京市产业结构特点和经济发展战略的需要,重点面向首都经济发展所需要的支柱产业、第三产业和新兴产业,以培养应用型人才为目标,根据经济和社会发展的需求,进行专业调整与建设,以满足市场对于各种专业人才的需要,形成... 详细信息

北京联合大学适应北京市产业结构特点和经济发展战略的需要,重点面向首都经济发展所需要的支柱产业、第三产业和新兴产业,以培养应用型人才为目标,根据经济和社会发展的需求,进行专业调整与建设,以满足市场对于各种专业人才的需要,形成了一个服务首都经济的全方位的教育体系。如何培养应用型人才是学校教学工作的重点。任何教学和科研活动都以此为目标开展工作。文章将从一个专业教师的视角,阐述应用型人才实践能力的培养模式。

关键词：应用型人才实践能力培养模式

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3.S MAX中体视动画生成模块的实现

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计算机工程 2002年第4期28卷 178-179,257页

作者：黄心渊陈世红北方工业大学CAD研究中心北京100041 北京联合大学应用文理学院北京100083

介绍了体视动画的生成方法,讨论了如何基于流行的三维动画软件3.S MAX开发体视动画的生成模块。最后给出了建筑动态模拟体视动画中的一帧图像。

关键词： 3.S MAX 三维动画软件体视动画生成模块计算机

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