Protein transduction domain of membrane penetrating peptide can efficiently deliver DNA and protein into mouse liver for gene therapy

作     者：Jun Xie, Bao-Feng Yu, Jun Xu, Yue-Hong Zhang, Niu-Liang Cheng, Bo Niu, Xiao-Nian Hu, Qian Xiang and Zheng-Guo Zhang Taiyuan, China Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001 , China Peking Union Medical College, Chi- nese Academy of Medical Sciences, Beijing 100005, China 

作者机构：Department of Biochemistry and Molecular Biology Shanxi Medical University Taiyuan 030001 China. xiejunty@*** 

出 版 物：《Hepatobiliary & Pancreatic Diseases International》 (国际肝胆胰疾病杂志（英文版）)

年 卷 期：2005年第4卷第1期

页      面：90-93页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：This study was supported by grants from the National Natural Science Foun-dation of China( No:30472251 )and the Shanxi Youth Science Fund ( No.020011028) 

主　　题：membrane penetrating peptide gene therapy gene delivering 

摘      要：BACKGROUND: The development of a harmless and effi- cient nonviral gene delivery system that can facilitate the penetration of nucleic acids through the plasma membrane is a key to successful gene therapy. The aim of this study was to test a nonviral gene transferring vector s function of delivering DNA into liver cells to provide an important clue for gene transfer in liver gene therapy. METHODS: The complex of DNA and DNA delivering protein was injected into mice through their tail veins. Then the mice were killed and their liver tissue was sec- tioned. The gene transferring results were detected using a confocal laser scanning microscope. RESULTS: Fluorescence analysis indicated that both DNA- membrane penetrating peptide (MPP) complex and DNA- hepatocyte specific receptor binding domain ( HSRBD) - MPP complex could go into liver cells. The fluorescence value of liver cells in the DNA-HSRBD-MPP group was higher than that in the DNA-MPP group. CONCLUSIONS; MPP can successfully deliver DNA and protein into cells, and MPP with a HSRBD can specifically deliver DNA into liver cells. These have laid a foundation for further study on the nonviral liver cell gene delivering system.