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Neuroglobin mutation associated with hypoxia adaptation in Tibet chicken

Progress in Natural science:Materials International 2007年第12期17卷 1419-1424页

作者： Zhang Lifan1, Liu Chong2. Lian Linsheng3, Zhao Chunjiang1, Bao Haigang1 and Wu Changxin1(1. college of animal {2. and technology, china agricultural university, beijing 100094, china 2. college of Biological {2., china agricultural university, beijing 100094, china 3. college of animal {2. and technology, Yunnan agricultural university, Kunming 6502.1, china) college of animal {2. and technology China Agricultural University Beijing 100094 China college of Biological science China Agricultural University Beijing 100094 China college of animal {2. and technology Yunnan Agricultural University Kunming 650201 China

Globin protein family plays an important role in storing and transporting oxygen. As a newly reported globin, the revealed function of neuroglobin includes binding and storing oxygen as well as facilitating the utilization of oxygen in neuronal cells. In the present study, mutations in exons of chicken Ngb gene were identified with the method of sequencing and created restriction site PCR in Tibet chicken and other four lowland chicken breeds. The mutations of Lys-2.2.(E4)-Asn and Ser-2.79(E4)-Gly were identified in exon 4 of the gene. The Lys-2.2.(E4)-Asn mutation existed only in Tibet chicken and the mutation frequencies increased with increasing altitude. Analysis of the haplotype and diplotype of the two mutations in Tibet chicken populations of different altitudes showed that the frequencies of TG haplotype and TTGG diplotype also increased with increasing altitude, while the reverse tendency was found on GGAA diplotype. Under the hypoxic simulation incubation, the main haplotype was TG in living embryos and GA in dead embryos. The results showed that the Lys-2.2.(E4)-Asn mutation may be a specific mutation associated with hypoxia adaptation in Tibet chicken.

关键词： Tibet chicken neuroglobin hypoxia adaptation Ngb gene mutation.

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Study on Tibetan Chicken embryonic adaptability to chronic hypoxia by revealing differential gene expression in heart tissue

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science china(Life sciences) 2009年第3期52卷 284-295页

作者： LI Mei & ZHAO ChunJiang college of animal {2. and technology,china agricultural university,beijing 100193,china 1. college of animal {2. and technology China Agricultural University Beijing 100193 China

Oxygen concentration is essential for appropriate *** can exert a significant impact on physiological alteration of the cell and *** Chicken(Gallus gallus) is a Chinese indigenous breed inhabiting in Tibetan areas,which is also a chicken breed living at high altitude for the longest time in the *** has developed an adaptive mechanism to hypoxia,which is demonstrated by that Tibetan Chicken has much higher hatchability than low-land chicken breeds in high-altitude areas of *** the present study,Tibetan Chicken fertilized full sib eggs were incubated up to Hamburger-Hamilton stage 43 under 13% and 2.% oxygen concentration,*** Chicken and Dwarf Recessive White Chicken were used as control *** hearts in all of the 3 chicken breeds under hypoxic and normoxic conditions were isolated and hybridized to Genechip Chicken Genome Array to study molecular mechanisms underlying the adaptation to high altitude of Tibetan *** a result,50 transcripts highly expressed in hypoxia are screened *** up-regulated genes,some are involved in the gene ontology(GO) such as cell growth,cell difference,muscle contraction and signal ***,the expression levels of 2. transcripts are lower in hypoxia than those in *** down-regulated genes take part in cell communication,ion transport,protein amino acid phosphorylation and signal ***,gene enrichment analyses of these differential gene expressions are mainly associated with immune system response and ion channel activity in response to ***,the transcriptional expression profiles analyzed by hierarchical clustering and CPP-SOM software in all of the 3 different chicken breeds revealed that Tibetan Chicken is much closely related to Shouguang Chicken rather than Dwarf Recessive White *** addition,12.transcripts of Tibetan Chicken breed-specific expressed genes were identified,which seem to result in a more

关键词： adaptation chronic hypoxia gene chip chicken genome array Tibetan Chicken(Gallus gallus) differential gene expression

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yellow^0,a marker for low body weight in Drosophila melanogaster

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science china(Life sciences) 2009年第7期52卷 672-682页

作者： Li XinHai & Deng XueMei college of animal {2. and technology,china agricultural university,beijing 100193,china 1. college of animal {2. and technology China Agricultural University Beijing 100193 China

Marker-assisted selection(MAS) is an important modern breeding technique,but it has been found that the effect of the markers for quantitative trait loci(QTL) is inconsistent,leading in some cases to MAS failure and raising doubts about its *** the model organism Drosophila melanogaster was employed to study whether an effective marker could be found and applied to *** crossed the stock carrying the y0 marker(a recessive mutation allele of the yellow gene on the X chromosome) with three other stocks carrying corresponding wild-type markers in an F2.design,and found that the y0 marker was in significant association with low body weight(P2. generations of marker-assisted introgression(MAI),and constructed the introgression stock y0(OR)2. in which body weight decreased by 13% and 7%,in female and male,respectively,compared to the wild stock(P2.,indicating that the QTL for low body weight is located within this less-than-1.5 cM *** characteristics of the y0 marker by PCR amplification and Southern blotting revealed that yellow gene was deficient in the y0 stock,leading to disappearance of melanin from the cuticle and probably influencing the developmental *** above results confirmed the existence of effective QTL markers applicable to MAS breeding schemes,and their potential application in breeding new stocks.

关键词： Drosophila low body weight yellow quantitative trait loci marker-assisted selection introgression

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Theoretical analysis of alloy multiplying factor by the empirical electron theory of solids and molecules

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science china(Technological sciences) 2007年第6期50卷 807-814页

作者： SONG YuePeng1,2. LIU GuoQuan2. LI ZhiLin3, FENG ChengMing1 1 Mechanical and Electronic Engineering college, Shandong agricultural {2., Tai’an 2.1018, china 2.college of Materials science and Engineering, {2. of science and technology, beijing 100083, china 3 college of Material science and Engineering, beijing {2. of Chemical technology, beijing 10002., china Mechanical Electronic Engineering college Shandong Agricultural University Tai’an 271018 China college of Materials science Engineering University of Science technology Beijing 100083 China college of Material science Engineering Beijing University of Chemical Technology Beijing 100029 China

By introducing the distribution probability of structural units in austenite contain- ing alloying elements and considering its effects on phase transformation, this paper establishes a calculation model of distribution probability of structural units. A new valence electron structure (VES) parameter-transformation effect coefficient of alloying elements (HL), is defined and then studied both theoretically and ex- perimentally. The relationship between the parameter HL and the multiplying factor (the quenching capability factor) of alloying elements is studied. The results indi- cate that the two parameters (HL and the quenching capability factor) have the same variation characteristic and substance feature. Therefore, the multiplying factor virtually expresses the relative quantity of structural units in the alloying elements-containing austenite.

关键词： multiplying factor (the quenching capability factor), empirical electron theory of solids and molecules, transformation effect coefficient of alloying elements, structural units in austenite containing alloying elements

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Boundary conditions for the bending of a piezoelectric beam

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science china(Physics,Mechanics & Astronomy) 2008年第7期51卷 847-856页

作者： GAO Yang1, XU SiPeng2.& ZHAO BaoSheng3 1 college of science,{2., beijing 100083, china 2.college of Engineering, Ocean university of china, Qingdao 2.6071, china 3 School of Mechanical Engineering, university of science and technology Liaoning, Anshan 114044, china 1. college of science China Agricultural University Beijing 100083 China 2. college of Engineering Ocean University of China Qingdao 266071 China 3. School of Mechanical Engineering University of Science and Technology Liaoning Anshan 114044 China

For beam bending in transversely isotropic piezoelectric media, the reciprocal theorem and the general solution of piezoelasticity are applied in a novel way to obtain the appropriate stress and mixed boundary conditions accurate to all orders for the beam of general edge geometry and loadings. By generalizing the method developed by Gregory and Wan, a set of necessary conditions on the edge-data for the existence of a rapidly decaying solution is established. The prescribed edge-data of the beam must satisfy these conditions in order that they could generate a decaying state within the beam. When stress and mixed conditions are imposed on the beam edge, these decaying state conditions for the case of bending deformation of piezoelectric beam are derived explicitly. They are then used for the correct formulation of boundary conditions for the beam theory solution (or the interior solution). Besides, an analytical solution of elastic beam is formulated to verify validity of our boundary conditions. For the stress data, our boundary conditions coincide with those obtained in conventional forms of beam theories. More importantly, the appropriate boundary conditions with two sets of mixed edge-data are obtained for the first time.

关键词： boundary conditions beam bending piezoelectric media decaying states regular states

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Mixed boundary conditions for piezoelectric plates

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science china(Physics,Mechanics & Astronomy) 2009年第5期52卷 755-761页

作者： GAO Yang 1 ,XU SiPeng 2.&ZHAO BaoSheng 3 1college of science,{2. agricultural university,beijing 100083,{2. 2.0} of Engineering,Ocean university of china,Qingdao 2.6071,{2. 3 School of Mechanical Engineering,university of science and technology of Liaoning,Anshan 114044,{2. college of science China Agricultural University Beijing 100083 China college of Engineering Ocean University of China Qingdao 266071 China School of Mechanical Engineering University of Science and Technology of Liaoning Anshan 114044 China

For plate bending and stretching problems in piezoelectric materials,the reciprocal theorem and the general solution of piezoelasticity are applied in a novel way to obtain the appropriate mixed boundary conditions accurate to all order.A decay analysis technique is used to establish necessary conditions that the prescribed data on the edge of the plate must satisfy in order that it should generate a decaying state within the *** the case of axisymmetric bending and stretching of a circular plate,these decaying state conditions are obtained explicitly for the first time when the mixed conditions are imposed on the plate *** are then used for the correct formulation of boundary conditions for the interior solution.

关键词： boundary conditions piezoelectric plates mixed data bending problems stretching problems

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Simulation study on the efficiencies of MOET nucleus breeding schemes applying marker assisted selection in dairy cattle

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science china(Life sciences) 2009年第3期52卷 296-306页

作者： LUO WeiZhen,WANG YaChun & ZHANG Yuan Department of animal Genetics & Breeding,college of animal science and technology,china agricultural university,beijing 100094,china 1. Department of animal Genetics & Breeding College of Animal Science and Technology China Agricultural University Beijing 100094 China

Advantages of breeding schemes using genetic marker information and/or multiple ovulation and embryo transfer(MOET) technology over the traditional approach were extensively evaluated through *** yield was the trait of interest and QTL was the genetic marker *** dairy cattle breeding scenarios were considered,i.e.,traditional progeny testing breeding scheme(denoted as STANPT),GASPT scheme including a pre-selection of young bulls entering progeny testing based on their own QTL information,MOETPT scheme using MOET technology to generate young bulls and a selection of young bulls limited within the full-sib family,GAMOPT scheme adopting both QTL pre-selection and MOET technology,COMBPT scheme using a mixed linear model which considered QTL genotype instead of the BLUP model in GAMOPT,and three non-progeny testing schemes,*** MOET,GAMO and COMB schemes,corresponding to MOETPT,GAMOPT and COMBPT with progeny testing being part of the *** were selected based on their breeding value which was estimated under an animal model *** selection over 17 years was performed in the simulations and 30 replicates were designed for each *** influences of using QTL information and MOET technology on favorable QTL allele frequency,true breeding values,polygenetic breeding values and the accumulated genetic superiority were extensively evaluated,for five different populations including active sires,lactating cows,bull dams,bull sires,and young *** results showed that the combined schemes significantly outperformed other approaches wherein accumulated true breeding value *** difference between schemes exclusively using QTL information or MOET technology was not *** STANPT scheme was the least efficient among the 8 *** schemes using MOET technology had a higher polygenetic response than others in the 17th *** increases of frequency of the favorable QTL allele varied more greatly across t

关键词： dairy cattle marker-assisted selection two-stage selection MOET QTL

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A five-fold pig bacterial artificial chromosome library:a resource for positional cloning and physical mapping

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Progress in Natural science:Materials International 2006年第08期16卷 889-892页

作者： LIU Wei, ZHANG Ying, LIU Zhaoliang, GUO Li, WANG Xiaobo, FEI Jing, FENG Jidong, ZHAO Rui, HU Xiaoxiang and LI Ning (The State Key Laboratory for Agro-Biotechnology, college of Biology,{2., beijing 100094, china college of animal science and technology,{2., beijing 100094, china) [a]The State Key Laboratory for Agno-Biotechnology College of Biology China Agricultural University Beijing China [b]college of animal {2. and technology China Agricultural University Beijing China

A pig BAC library was constructed with genomic DNA from a male Erhualian pig. After partial digestion with HindⅢor BamHⅠthe fragments obtained were cloned into the pBeloBAC11 vector. The library consists of 18432. clones which stored in 480 pieces 384-well plates (2. plates per superpool). A two-step 4-dimension PCR screening system was established to screen the positive clones. An average insert size of 12. kb was estimated from 105 randomly isolated clones, which indicates that the library is more than five times of genomic coverage. For the demonstration of the probability to pick out any unique genes or DNA markers from the library, 10 single-copy genes were screened out and the positive clones were yielded between 1 and 8 with an average of 3.6. Positive superpools were obtained for 32.microsatellite markers selected from different regions of pig genome. The number of positive superpools for each marker varies from 1 to 9 with an average of 4.78. This BAC library provides an additional resource for pig physical mapping and gene identifica-

关键词： pig BAC library PCR screening system.

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Phototoxic effect of UVR on wild type, ebony and yellow mutants of Drosophila melanogaster: Life Span, fertility, courtship and biochemical aspects

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science china(Life sciences) 2008年第10期51卷 885-893页

作者： WANG ZhePeng1,2. LIU RuiFang2. WANG AnRu3, DU LiLi1 & DENG XueMei1 1 college of animal {2. and technology, china agricultural university, beijing 100094, china 2.Vocational and Technologic college, Inner Mongolia Agriculture university, Baotou 014109, china 3 college of Veterinary Medicine, china agricultural university, beijing 100094, china 1. college of animal {2. and technology China Agricultural University Beijing 100094 China 2. Vocational and Technologic college Inner Mongolia Agriculture University Baotou 014109 China 3. college of Veterinary Medicine China Agricultural University Beijing 100094 China

Melanin plays an important role in protecting organisms from ultraviolet radiation (UVR). Therefore, it is possible that differently colored strains can show different sensitivities to UVR. In the present work, life span, fertility and courtship behavior of wild type (w), ebony (e) and yellow (y) strains of Drosophila melanogaster were studied to evaluate their sensitivity to ultraviolet (UV). Because a range of photo- toxic effects of UVR are mediated through generation of free radicals, levels of free radicals, lipid per- oxide (malondialdehyde, MDA) and superoxide dismutase (SOD) activity of three strains were examined to indicate their antioxidant defending ability and oxidative status. It was shown that w always had the highest lifespan and fertility not only in the control but also in UV-exposed groups. Moreover, lifespan and fertility of e were significantly higher (P2. effect on the SOD activities. After pooling data from the control and UV-exposed groups, we found that w had a significantly (P0.05). MDA levels were increased in the UV dose-dependent manner (P=0.0495). In con- clusion, our results suggested that UVR can decrease life span and fertility of flies and do harm to courtship, which may be due to oxidative damage to flies tissues (e.g. central nervous system) induced by free radicals. w had the highest tolerance to UVR, which may be ascribed to its advantage of survival under the natural condition and at high level of SOD activity. Then differences of pigment between e and y in absorbing UV, shielding agai

关键词： ultraviolet radiation Drosophila melanogaste free radicals pigment

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Purification of a Novel Antibacterial Short Peptide in Earthworm Eisenia foetida

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 297-302页

作者： Yan-Qin LIU1,2. Zhen-Jun SUN1*, Chong WANG1, Shi-Jie LI2. and Yu-Zhi LIU2.college of Resource and Environment science,{2., beijing 100094, china 2.0} of animal science, Hebei agricultural university, Baoding 071001, china college of Resource and Environment science China Agricultural UniversityBeijing 100094 China college of animal {2. Hebei Agricultural UniversityBaoding 071001 China

A novel antimicrobial short peptide was purified from earthworm (Eisenia foetida) by a five-step protocol including ammonium sulfate precipitation, ultrafiltration, DE-52.ion exchange chromatography, Sephadex G-10 column chromatography, and C-18 reversed-phase HPLC techniques. The purified peptide was applied to the MALDI-TOF MS to determine the molecular mass and was also subjected to TOF MS-MS analysis to determine the amino acid sequence. As a result, a novel antibacterial peptide, named OEP312., was obtained, with the molecular mass of 510.8 Da and the sequence being “ACSAG”.

关键词： antibacterial peptide earthworm purification

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