检索结果-南通市图书馆

Influences of Quinclorac on Culturable Microorganisms and Soil Respiration in Flooded Paddy Soil

Biomedical and Environmental sciences 2003年第4期16卷 314-322页

作者： ZHEN-MEI LU, HANG MIN , AND YANG-FANG YE Institute of Microbiology, college of life science, Zhejiang university, Hangzhou 310029, Zhejiang, china Institute of Microbiology College of Life Science Zhejiang University Hangzhou 310029 Zhejiang China

Objective To investigate the potential effects of herbicide quinclorac (3,7-dichloro-8-quinoline-carboxylic) on the culturable microorganisms in flooded paddy soil. Methods Total soil aerobic bacteria, actinomycetes and fungi were counted by a 10-fold serial dilution plate technique. Numbers of anaerobic fermentative bacteria (AFB), denitrifying bacteria (DNB) and hydrogen-producing acetogenic bacteria (HPAB) were numerated by three-tube anaerobic most-probable-number (MPN) methods with anaerobic liquid enrichment media. The number of methanogenic bacteria (MB) and nitrogen-fixing bacteria (NFB) was determined by the rolling tube method in triplicate. Soil respiration was monitored by a 102G-type gas chromatography with a stainless steel column filled with GDX-104 and a thermal conductivity detector. Results Quinclorac concentration was an important factor affecting the populations of various culturable microorganisms. There were some significant differences in the aerobic heterotrophic bacteria. AFB and DNB between soils were supplemented with quinclorac and non-quinclorac at the early stage of incubation, but none of them was persistent. The number of fungi and DNB was increased in soil samples treated by lower than 1.33μg·g-1 dried soil, while the CFU of fungi and HPAB was inhibited in soil samples treated by higher than 1.33μg·g-1 dried soil. The population of actinomycete declined in negative proportion to the concentrations of quinclorac applied after 4 days. However, application of quinclorac greatly stimulated the growth of AFB and NFB. MB was more sensitive to quinclorac than the others, and the three soil samples with concentrations higher than 1 μg·g-1 dried soil declined significantly to less than 40% of that in the control, but the number of samples with lower concentrations of quinclorac was nearly equal to that in the control at the end of experiments. Conclusion Quinclorac is safe to the soil microorganisms when applied at normal concentrations (0.67

关键词： Quinclorac Soil culturable microorganisms Soil respiration Flooded paddy soil

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Peplomycin induces G1-phase specific apoptosis in liver carcinoma cell line Bel-7402 involving G2-phase arrest

引用

Acta Pharmacologica Sinica 2004年第12期25卷 132-138页

作者： Hong-ying CHEN2, Cong-yi ZHENG2, Guo-lin ZOU2, Da-xing XIE3, Jian-ping GONG3 2college of life sciences, Wuhan university, Wuhan 430072 3Cancer Research Institute, Tongji Hospital, TongjiMedical college, Huazhong university of science and Technology, Wuhan 430072, china college of life sciences Wuhan University Wuhan 430072 China

AIM: To investigate the mechanism of peplomycin (PEP)-induced apoptosis in liver carcinoma cell line (Bel-7402). METHODS: Growth inhibition by PEP was analyzed using 3- 4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptotic cells were detected using Hoechest 33258 staining, and confirmed by flow cytometric analysis and DNA fragmentation analysis. The expression of cyclin A and B1 were determined by flow cytometry and Western blot. Annexin V assay was measured by flow cytometric analysis. RESULTS: PEP induced apoptosis and then inhibited cell proliferation in liver carcinoma cell line Bel-7402. Cells treated with PEP 50 μmol/Lfor 15 h were arrested in G2-phase with dramatical expression of cyclin A and a little change in cyclin B1. Almost all the apoptosis occurred in cells undergoing the G1-phase after treatment for 24 h. CONCLUSION: Peplomycin induced G1-phase specific apoptosis in Bel-7402 involving G2-phase arrest.

关键词： peplomycin Bel-7402 cells apoptosis cyclins cell cycle flow cytometry

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Mine laneway 3D reconstruction based on photogrammetry

引用

中国有色金属学会会刊：英文版 2011年第S3期21卷 686-691页

作者： ZHANG Chun-sen college of Geomatics, Xi’an university of science and Technology, Xi’an 710054, china college of Geomatics Xi'an University of Science and Technology Xi'an 710054 China

Aiming at the problem of 3D reconstruction for mine laneway, the technique route and step were given based on digital close range photogrammetry and traverse surveying. 3D coordinates of laneway traverse point, photogrammetric station, control strip points were obtained by electronic total station based on mine coordinate system. The feature points were extracted from the scene images. The camera was calibrated by direct linear transformation (DLT). 3D relative coordinates of the arbitrary feature points in mine laneway were calculated after feature points being matched, in which the origin of coordinate system is photogrammetric station. The 3D absolute coordinates of the feature points in mine laneway can be obtained by the combined adjustment of photogrammetry and non-photogrammetric observations and coordinate conversion. The measurable 3D model of the underground laneway terrain surface was constructed with the 3D coordinates of feature points on the image pairs obtained. The measurable 3D model of a corridor was reconstructed by the mentioned method and the experiment precision with an average standard deviation of 0.013 6 m between the 3D model and practical measure was obtained by electronic total station. The experimental resulted show the validity of the proposed approach.

关键词： digital mine mine laneway photogrammetry 3D reconstruction direct linear transformation (DLT)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

New procedure for the differential display of mitochondria RNA

引用

Chinese science Bulletin 1999年第16期44卷 1502-1505页

作者： LING Xingyuan, ZHOU Peijiang and ZHU Yingguo1. Institute of Genetics , college of life science , Wuhan university , Wuhan 430072, china.2. The Center of Biotechnology , Anhui agriculture university , Hefei 230036, china.Corresponding author Institute of Genetics College of Life Science Wuhan University Wuhan China The Center of Biotechnology Anhui Agriculture University Hefei China

A novel approach for the differential display of mitochondria RNA has been described . The total mitochondria RNAs are ligated to a single-strand linker with T4 RNA ligase and then reversely transcripted into the first strands of cDNA. The first strands of cDNA that are ligated to another single-strand linker also with T4 RNA ligase can be directly used as the tem-

关键词： rice mitochondria RNA. mRNA differential display. amplification fragment length polymorphism (AFLP ) .

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phototoxic effect of UVR on wild type, ebony and yellow mutants of Drosophila melanogaster: life Span, fertility, courtship and biochemical aspects

引用

science china.life sciences) 2008年第10期51卷 885-893页

作者： WANG ZhePeng1,2, LIU RuiFang2, WANG AnRu3, DU LiLi1 & DENG XueMei1 1 college of Animal science and Technology, china.Agricultural university, Beijing 100094, china.2 Vocational and Technologic college, Inner Mongolia agriculture university, Baotou 014109, china.3 college of Veterinary Medicine, china.Agricultural university, Beijing 100094, china 1. college of Animal science and Technology China Agricultural University Beijing 100094 China 2. Vocational and Technologic college Inner Mongolia Agriculture University Baotou 014109 China 3. college of Veterinary Medicine China Agricultural University Beijing 100094 China

Melanin plays an important role in protecting organisms from ultraviolet radiation (UVR). Therefore, it is possible that differently colored strains can show different sensitivities to UVR. In the present work, life span, fertility and courtship behavior of wild type (w), ebony (e) and yellow (y) strains of Drosophila melanogaster were studied to evaluate their sensitivity to ultraviolet (UV). Because a range of photo- toxic effects of UVR are mediated through generation of free radicals, levels of free radicals, lipid per- oxide (malondialdehyde, MDA) and superoxide dismutase (SOD) activity of three strains were examined to indicate their antioxidant defending ability and oxidative status. It was shown that w always had the highest lifespan and fertility not only in the control but also in UV-exposed groups. Moreover, lifespan and fertility of e were significantly higher (P0.05). MDA levels were increased in the UV dose-dependent manner (P=0.0495). In con- clusion, our results suggested that UVR can decrease life span and fertility of flies and do harm to courtship, which may be due to oxidative damage to flies tissues (e.g. central nervous system) induced by free radicals. w had the highest tolerance to UVR, which may be ascribed to its advantage of survival under the natural condition and at high level of SOD activity. Then differences of pigment between e and y in absorbing UV, shielding agai

关键词： ultraviolet radiation Drosophila melanogaste free radicals pigment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

FRUIT NUTRITIVE FACTOR NETWORK

引用

Journal of Systems science & Complexity 2009年第1期22卷 150-158页

作者： Daren HE college of Physics science and Technology Yangzhou University

As an research example of the widely existing cooperation-competition systems,the authorspresent an empirical investigation on a fruit nutritive factor *** is described by a node-weightedbipartite *** fruit nutritive factors are defined as the nodes,and two nodes are connected by anedge if at least one fruit contains these two nutritive *** fruits are defined as the *** node-weight W_(nt),which signifies the 'importance degree' of each actor node,is defined as thecont ent of a nutritive factor in a *** empirical investigation results show some unique *** node-weight distributions take so-called 'shifted power law' function forms,but the act-weightdistribution takes a normal *** degree and act-degree distributions show impulsive-spectrumlike *** observations may be helpful for the study of *** network description methodproposed in this article may be universal for a kind of cooperation-competition systems.

关键词： Complex network cooperation-competition nntritive factor statistical properties.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Immunohistochemical localization of Ca^(2+)/calmodulin-dependent kinase in tobacco

引用

Chinese science Bulletin 2001年第3期46卷 230-233页

作者： YANG Wannian, LIANG Shuping & LU YingtangCenter of Developmental Biology, college of life science, Wuhan university, Wuhan 430072, china Center of Developmental Biology College of Life Science Wuhan University Wuhan China

The existence of Ca2+/calmodulin-dependent kinase (CaM kinase, CaMK) in tobacco is verified immuno-logically and its distribution in different tissues of tobacco is studied. It has been demonstrated that CaMK is mainly distributed in early developing anthers, developing ovules and embryos, lateral root primordium, apical meristem and leaf primordium of buds and mesophyll cells and developing vascular bundles of leaves. There is enormous CaM kinase distributed in leaf epidermis fair cells and guard cells of stomas too. Little kinase is found in mature stem or root cells. The distribution properties of CaM kinase in tobacco are consistent with those of CaM, suggesting that there exists the Ca2+ signal transduction pathway mediated by CaM kinase in tobacco and it plays an important role in the plant growth and development.

关键词： CaM kinase immunohistochemical localization tobacco anthers.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Preliminary study on seasonal succession and development pathway of phytoplankton community in the Bohai Sea

引用

Acta Oceanologica Sinica 2001年第2期20卷 251-260页

作者： Sun Jun, Liu Dongyan, Qian Shuben (1. Marine Ecosystem Dynamic Lab, Marine life science college, Ocean university of Qingdao, Qingdao 266003, china. ) Marine Ecosystem Dynamic Lab Marine Life Science College Ocean University of Qingdao Qingdao 266003 China

Phytoplankton species composition and species succession were determined in 1998-1999 based on 2 nestle investigation cruises in the Bohai Sea and two monthly monitoring stations at Penglai and Changdao for 15 months. The seasonal succession and pathway of phytoplankton community in the Bohai Sea were discussed complementarily with history data. The main process of Phytoplankton community development in the Bohai Sea was controlled by temperature and nutrient replenishes. There were two cell abundance peaks in an annual variation, the main peak in April and the secondary peak in September. In winter, the cell abundance was low due to the low temperature, the phytoplankton community was mainly made up of small-celled diataoms. In spring, the phytoplankton community was developed very quickly by small-celled diatom in suitable conditions of temperature and nutrients. In summer, the cell abundance decreased and big-celled diatoms became predominated. In autumn, because of the replenish of nutrient, big-celled diatoms and dinoflagellates formed another cell abundance peak. during the annual variation of phytoplankton community in the Bohai Sea, species succession was the main process of community development, the species sequence just occur at special areas and special periods. The evolution of phytoplankton community in the Bohai Sea accords with the hypothesis of Margalef's phytoplankton community of four stages. But the size feature is contrary to the hypothesis, which may be caused by nutrient replenish in autumn in Bohai Sea and the top to down control.

关键词： Phytoplankton community seasonal changes species succession species sequence the Bohai Sea

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

b-DAZL:A novel gene in bovine spermatogenesis

引用

Progress in Natural science:Materials International 2008年第10期18卷 1209-1218页

作者： Qingbo Zhang~a Qifa Li~(a,*) Jiahuang Li~b Xinfu Li~a Zhenshan Liu~a Dawei Song~a Zhuang Xie~a ~a Laboratory of Animal Reproductive Genetics and Molecular Evolution,college of Animal science and Technology,Nanjing agriculture university,Nanjing 210095,china.~b Pharmaceutic Biotechnology Key Laboratory,college of life science,Nanjing university,Nanjing 210093,china Laboratory of Animal Reproductive Genetics and Molecular Evolution College of Animal Science and Technology Nanjing Agriculture University Nanjing 210095 China Pharmaceutic Biotechnology Key Laboratory College of Life Science Nanjing University Nanjing 210093 China

The DAZL gene plays an important role in *** and mutation of this gene has been frequently associated with the sterility in both vertebrates and ***,a cDNA containing the complete open reading frame of DAZL gene,which encodes putative RRM RNA-binding protein,was isolated from bovine tissues by *** isolated gene was referred to as ***-based location analysis showed that b-DAZL is located near the end of chromosome *** b-DAZL gene and its vertebrate homologue shared a high nucleotide similarity (90-94%) in the coding region and in the genomic organization,each consisting of 10 exons and 9 *** protein products contained a highly conserved RNA-binding motif,a unique DAZ repeat, and PUM2 and PABP interacting ***-PCR assay revealed that b-DAZL was expressed in the testis and ***,no signals were detected in the testes of sterile male *** microstructure of the testes of sterile males showed very few spermat- ocytes but mostly somatic *** observations are consistent with the typical phenotype of testes with defective DAZL expression. Subsequent gene detection studies indicated that transcription arrest might lead to a b-DAZL transcript defect in testes of cattle-yaks. Our results suggest that b-DAZL may be involved in spermatogenesis,and that transcription arrest of the gene is associated with male sterility in cattle-yaks.

关键词： Cattle-yak b-DAZL Male sterility Sequence analysis Expression profile

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

SOME NEW PROPERTIES ON Lp（Ω,H~n）

引用

Applied Mathematics:A Journal of Chinese Universities 2007年第2期 174-180页

作者： Jia Gao college of science, university of Shanghai for science and Technology, Shanghai 200093, china.

Let Lp(Ω,Hn) indicate the Lp-space of the maps for Heisenberg group target. In this paper some new properties are obtained for the space Lp(Ω, Hn).

关键词： Heisenberg group Lp-space quasi-center of mass.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：