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Competitive binding of postsynaptic density 95 and Ca2+-calmodulin dependent protein kinase II to N-methyl-D-aspartate receptor subunit 2B in rat brain

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Acta Pharmacologica Sinica 2004年第2期25卷 50-54页

作者： Fan-jie MENG , Jun GUO, Bo SONG, Xue-bo YAN, Guang-yi ZHANG2 Research center of Biochemistry and Molecular biology, Xuzhou Medical College, Xuzhou 221002, China Research center of Biochemistry and Molecular biology Xuzhou Medical College Xuzhou 221002 China

AIM: To investigate the interactions among postsynaptic density 95 (PSD-95), Ca2+-calmodulin dependent protein kinase IIα (CaMKIIα), and N-methyl-D-aspartate receptor subunit 2B (NR2B) during ischemia and reperfusion in hippocampus of rats. METHODS: Brain ischemia was induced by four-vessel occlusion procedure in rats. Immu- noprecipitation and immunoblotting were performed to study the interactions and phosphorylation of proteins. The association-dissociation of PSD-95 and CaMKIIα to and from N-methyl-D-aspartate (NMDA) receptor induced by ischemia and reperfusion and the effects of 1-[N,O-bis-(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenyl-pip- erazine (KN-62, a selective inhibitor of CaMKII) on these protein interactions were investigated. Coimmuno- precipitation and immunoblotting were performed for the studies of interactions among proteins. RESULTS: The alternations of the binding level of PSD-95 and CaMKIIα to NR2B during ischemia and reperfusion demonstrated the negative correlation to each other. Pre-administration of KN62 through both cerebral ventricles inhibited the 10 min ischemia-induced increase of the binding of PSD-95 to NR2B and, on the contrary, promoted the binding of CaMKIIα to NR2B. CONCLUSION: PSD-95 competes with CaMKII to bind to NR2B during ischemia and reperfusion in rat hippocampus.

关键词： N-methyl-D-aspartate receptors Ca2+-calmodulin dependent protein kinase brain ischemia

激光诱变遗传的共振激发及非线性作用机理分析

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中国激光 1998年第1期25卷 91-95页

作者：张玲琪陈有为周凌云魏蓉城云南大学生物系云南大学省微生物研究所昆明理工大学非线性科学研究中心

用量子力学及非线性科学理论，研究了激光与脱氧核糖核酸（ＤＮＡ）分子相互作用的共振吸收效应及混沌性质，进而对激光的生物诱变效应予以解释。

关键词：激光诱变 DNA 共振吸收混沌诱变

Bolt-like Conformation of HIV-1 Fusion Peptide

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Bolt-like Conformation of HIV-1 Fusion Peptide

Molecular regulation of cardiovascular cell growth and apoptosis in atherosclerotic coronary artery disease

中国遗传学会七届一次青年研讨会暨上海高校模式生物E——研究院第一届模式生物学术研讨会

作者： Zhang Wen,Cao Huai, Liu Ciquan (Department of biology, Kunming Medical College, Kunming 650031 Modern biology Research center, Yunnan University, Kunming 650091)

During the invasion of HTV-1, the fusion pepdde mediates the fusion between virus envelope and host cell membrane. In this study, we analyzed the mutation of HIV-1 fusion peptides in sequenced HIV-1 genomes. Based on the prediction of the secondary structure of consensus HTV-1 fusion peptide, we modeled the 3D conformation of the fusion peptide with molecular mechanics force fields and lead to a bolt-like conformation and functional mechanism.

关键词： fusion peptide HTV-1 molecular mechanics

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Molecular regulation of cardiovascular cell growth and apopt...

Species composition and distribution of marine nematode community in the North Taiwan Strait

中国病理生理学会第九届全国代表大会及学术会议

作者： Yong-Jian Geng (The center for Cardiovascular biology and Atherosclerosis Research,University of Texas Health Science center at Houston, and Texas Heart Institute,Houston,USA.)

＜正＞A balance between growth and apoptosis plays a critical role in maintaining the homeostasis of cardiovascular *** the development of atherosclerosis and atherosclerosis-associated coronary heart disease,many environmental factors exert regulatory effects on cardiovascular cells and their *** have recently investigated several proteins known to protect cells against ische-

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Acta Oceanologica Sinica 2001年第2期20卷 221-230页

作者： Cai Lizhe, Hong Huasheng, Zou Chaozhong, Fang Shaohua, Lu Xiaomei, Zhang Yueping (1. Key Laboratory of SEDC of Marine Environmental Science/Environmental Science Research center, Xiamen Universi- ty, Xiamen 361005, China 2. Department of biology, Xiamen Key Lab. of SEDC of Marine Envrn. Sc Xiamen University Xiamen 361005 China

Marine free-living nematodes were investigated at 13 sampling stations divided into three transects in the northern Taiwan Strait in February 1998. One hundred species of marine nematodes belonging to 91 Genera 28 Families 3 Orders were identified and were first recorded in the northern Taiwan Strait. The dominant species were Vasostoma sp., Sabatieria sp. 1, Linhystera sp. 1, Spilophorella sp., Daptonema sp., Halalaimus sp. and Dorylaimopsis variabilis. Their main densities were all over 4 950 ind./m2. According to mean density at transects, marine nematode density decreased from coastal Weitou to off Minjiang Estuary, which was similar to polychaete distribution in northern Taiwan Strait. The selective deposit feeder (1A) was the dominant food type of marine nematodes in the northern Taiwan Strait, but non-selective deposit feeders (1B) and epigrowth feeders (2A) occupied high proportion, indicating diverse feeding types of marine nematodes in the northern Taiwan Strait. Some environmental factors such as currents are discussed.

关键词： marine nematode benthonic ecology the Taiwan Strait

Blood Compatibility of Nano-Hydroxyapatite Dispersed Using Various Agents

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Wuhan University Journal of Natural Sciences 2010年第4期15卷 350-354页

作者： YANG Lijian1, JIN Huafang2, YUAN Lin2 1. Hospital, Wuhan University of Technology, Wuhan 430070, Hubei, China 2. biology center,Wuhan University of Technology, Wuhan 430070, Hubei, China Hospital Wuhan University of Technology Wuhan Hubei China biology center Wuhan University of Technology Wuhan Hubei China

Nano-hydroxyapatite (nHAP),dispersed with three kinds of dispersants (heparin sodium,polyacrylic sodium and wa-ter),reacted with red blood cell,Bel-7402 tumor cell,to compare their dispersing efficiency against nHAP from one another. The blood compatibility of nHAP is also determined by blood solubil-ity experiment so that the capability of different dispersant dis-persing against nHAP of different concentration and the relation between nHAP and blood compatibility have been determined. The inhibiting function of Bel-7402 against tumor cells is deter-mined with the MTT staining method. The study result shows that heparin sodium has the best dispersing efficiency for nHAP with-out the phenomenon of hemolysis.

关键词： nano-hydroxyapatite heparin sodium polyacrylic sodium blood compatibility

Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes

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Cell Research 2003年第4期13卷 251-263页

作者： YING CHEN, ZHI Xu HE, AILIAN LIU, KAI WANG, WEN WEI MAO, JIAN XIN CHU, YONG LU, ZHENG Fu FANG, YING TANG SHI, QING ZHANG YANG, DA YUAN CHEN, MIN RANG WANG, JIN SONG LI, SHAO LIANG HUANG, XIANG YIN KONG, YAO ZHOU SHI, Zm QIANG WANG, JIA HuI XIA, ZHI GAO LONG, ZHI GANG XUE, WEN XIANG DING, HuI ZHEN SHENG center for Developmental biology, Shanghai Second Medical University, Shanghai 200092, China Laboratory of Stem Cell biology, Institute of Biochemistry and Cell biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 1665 Kong Jiang Road, Shanghai 200092, China center for Stem Cell Research, the Second Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510120,China State Key Laboratory of Reproductive biology, Institute of Zoology, Chinese Academy of Sciences, 19 Zhong-guan Cun Road, Haidian District, Beijing 100080, China Health Science center, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences and Shanghai Second Medical University, Shanghai 200025, China National Laboratory of Medical Genetics, Central South University, 88 Xiangya Road, Changsha 410078, China Xinhua Hospital, Shanghai Second Medical University, 1665 Kong Jiang Road, Shanghai 200092, China 2Laboratory of Stem Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 3center for Stem Cell Research the Second Affiliated Hospital Sun Yat-Sen University Guangzhou 510120 China. 2Laboratory of Stem Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 4State Key Laboratory of Reproductive biology Institute of Zoology Chinese Academy of Sciences 19 Zhong-guan Cun Road Haidian District Beijing 10008

To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PCR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well

关键词： nuclear transfer (NT) somatic cell nuclear transfer (SCNT) embryonic stem cells (ES cell) therapeutic cloning rabbit oocyte.

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延缓衰老相关的小分子物质研究进展

生物化学与生物物理进展 2010年第9期37卷 932-938页

作者：高凌云李国栋童坦君北京大学医学部生物化学与分子生物学系北京大学衰老研究中心北京100191

筛选、研究具有延缓衰老作用的小分子物质,对于发现新的治疗衰老相关性疾病及肿瘤等的有效靶点、开发新型药物、促进人类健康具有重大的现实意义.同时更重要的是,可以这些小分子物质为切入点,对衰老、肿瘤等生命现象的具体分子机制进行... 详细信息

筛选、研究具有延缓衰老作用的小分子物质,对于发现新的治疗衰老相关性疾病及肿瘤等的有效靶点、开发新型药物、促进人类健康具有重大的现实意义.同时更重要的是,可以这些小分子物质为切入点,对衰老、肿瘤等生命现象的具体分子机制进行深入研究,这对于分子生物学等相关生命科学研究的发展具有重要的推动作用.总结了近一二十年来发现的一些具有代表性的可延缓衰老的小分子物质,并重点论述了其作用的分子机制.

关键词：衰老 SIRT1 晚期糖基化终末产物 TOR 热量限制药物肿瘤

Expression of a Modified Cry1Ie Gene in E. coli and in Transgenic Tobacco Confers Resistance to Corn Borer

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 309-313页

作者： Yun-Jun LIU, Fu-Ping SONG1, Kang-Lai HE1, Yuan YUAN, Xiao-Xia ZHANG, Peng GAO, Jian-Hua WANG, and Guo-Ying WANG * State Key Laboratory for Agrobiotechnology, National center for Maize Improvement, China Agricultural University, Beijing 100094, China 1State Key Laboratory for biology of Plant Diseases and Insect Pests, Institute of Plant Protection, CAAS, Beijing 100094, China State Key Laboratory for Agrobiotechnology National Center for Maize Improvement China Agricultural UniversityBeijing 100094 China State Key Laboratory for biology of Plant Diseases and Insect Pests Institute of Plant ProtectionCAAS Beijing 100094 China

The wild-type Cry1Ie gene from Bacillus thuringiensis was modified for its efficient expression in transgenic plants. Modified Cry1Ie gene (designated as Cry1Iem) was cloned into prokaryotic expression vector pET28b and its expression in E. coli was confirmed by SDS-PAGE analysis. Bioassays using crude expression products in E. coli revealed that Cry1Iem protein had a similar toxicity to corn borer as wild-type Cry1Ie. Cry1Iem gene was then inserted downstream of the maize ubiquitin-1 promoter in plant expression vector p3301. Transgenic tobacco plants carrying Cry1Iem showed insecticidal activity against corn borer.

关键词： Bacillus thuringiensis Cry1Iem transgenic tobacco insect-resistance