Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes

作     者：YING CHEN, ZHI Xu HE, AILIAN LIU, KAI WANG, WEN WEI MAO, JIAN XIN CHU, YONG LU, ZHENG Fu FANG, YING TANG SHI, QING ZHANG YANG, DA YUAN CHEN, MIN RANG WANG, JIN SONG LI, SHAO LIANG HUANG, XIANG YIN KONG, YAO ZHOU SHI, Zm QIANG WANG, JIA HuI XIA, ZHI GAO LONG, ZHI GANG XUE, WEN XIANG DING, HuI ZHEN SHENG Center for Developmental Biology, Shanghai Second Medical University, Shanghai 200092, China Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 1665 Kong Jiang Road, Shanghai 200092, China Center for Stem Cell Research, the Second Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510120,China State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 19 Zhong-guan Cun Road, Haidian District, Beijing 100080, China Health Science Center, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences and Shanghai Second Medical University, Shanghai 200025, China National Laboratory of Medical Genetics, Central South University, 88 Xiangya Road, Changsha 410078, China Xinhua Hospital, Shanghai Second Medical University, 1665 Kong Jiang Road, Shanghai 200092, China 

作者机构：Center for Developmental Biology Shanghai Second Medical University Shanghai 200092 China Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China Center for Stem Cell Research the Second Affiliated Hospital Sun Yat-Sen University Guangzhou 510120 China State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences 19 Zhong-guan Cun Road Haidian District Beijing 100080 China Health Science Center Shanghai Institutes of Biological Sciences Chinese Academy of Sciences and Shanghai Second Medical University Shanghai 200025 China National Laboratory of Medical Genetics Central South University 88 Xiangya Road Changsha 410078 China Xinhua Hospital Shanghai Second Medical University 1665 Kong Jiang Road Shanghai 200092 China 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2003年第13卷第4期

页      面：251-263页

核心收录：

学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：国家重点基础研究发展计划(973计划)(001CB5099) 国家高技术研究发展计划(863计划)(2001AA216121) 国家自然科学基金(30040003) Projects of Shanghai Science & Technology Development Foundation(99DJ14002,00DJ14033,01DJ14003) 中国科学院资助项目(KSCX-2-3-08) Shanghai Second Medical University 

主　　题：nuclear transfer (NT) somatic cell nuclear transfer (SCNT) embryonic stem cells (ES cell) therapeutic cloning rabbit oocyte. 

摘      要：To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PCR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well